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UniProtKB - Q9HJH3 (GGGPS_THEAC)
Protein
Geranylgeranylglyceryl phosphate synthase
Gene
Ta0995
Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Functioni
Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids. Cannot use sn-glycerol-3-phosphate (G3P) as substrate.
2 PublicationsCatalytic activityi
- (2E,6E,10E)-geranylgeranyl diphosphate + sn-glycerol 1-phosphate = diphosphate + sn-3-O-(geranylgeranyl)glycerol 1-phosphateUniRule annotation2 PublicationsEC:2.5.1.41UniRule annotation2 Publications
Cofactori
Mg2+UniRule annotation1 PublicationNote: Cannot use Mn2+ or Zn2+.1 Publication
Activity regulationi
Inhibited by high concentrations of magnesium (>10 mM) and by EDTA in vitro.1 Publication
Kineticsi
- KM=21.2 µM for G-1,3-P (at 55 degrees Celsius and pH 7)1 Publication
- KM=75 nM for GGPP (at 55 degrees Celsius and pH 7)1 Publication
- Vmax=13.5 µmol/min/mg enzyme (at 55 degrees Celsius and pH 7)1 Publication
pH dependencei
Optimum pH is about 7.0. Active from pH 6 to 9.1 Publication
Temperature dependencei
Optimum temperature is 55 degrees Celsius.1 Publication
: glycerophospholipid metabolism Pathwayi
This protein is involved in the pathway glycerophospholipid metabolism, which is part of Membrane lipid metabolism.UniRule annotationView all proteins of this organism that are known to be involved in the pathway glycerophospholipid metabolism and in Membrane lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 23 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 52 | MagnesiumUniRule annotation | 1 |
GO - Molecular functioni
- magnesium ion binding Source: UniProtKB-UniRule
- phosphoglycerol geranylgeranyltransferase activity Source: UniProtKB-UniRule
GO - Biological processi
- glycerophospholipid biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
SABIO-RKi | Q9HJH3 |
UniPathwayi | UPA00940 |
Names & Taxonomyi
Protein namesi | Recommended name: Geranylgeranylglyceryl phosphate synthaseUniRule annotation (EC:2.5.1.41UniRule annotation2 Publications)Short name: GGGP synthaseUniRule annotation Short name: GGGPSUniRule annotation Alternative name(s): (S)-3-O-geranylgeranylglyceryl phosphate synthaseUniRule annotation Phosphoglycerol geranylgeranyltransferaseUniRule annotation |
Gene namesi | Ordered Locus Names:Ta0995 |
Organismi | Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) |
Taxonomic identifieri | 273075 [NCBI] |
Taxonomic lineagei | Archaea › Candidatus Thermoplasmatota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000138746 | 1 – 253 | Geranylgeranylglyceryl phosphate synthaseAdd BLAST | 253 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsProtein-protein interaction databases
STRINGi | 273075.Ta0995 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q9HJH3 |
SMRi | Q9HJH3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 171 – 177 | Glycerol-1-phosphate bindingUniRule annotation | 7 | |
Regioni | 202 – 203 | Glycerol-1-phosphate bindingUniRule annotation | 2 | |
Regioni | 224 – 225 | Glycerol-1-phosphate bindingUniRule annotation | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | arCOG01085, Archaea |
HOGENOMi | CLU_068610_0_0_2 |
OMAi | ADAIFFM |
Family and domain databases
CDDi | cd02812, PcrB_like, 1 hit |
Gene3Di | 3.20.20.390, 1 hit |
HAMAPi | MF_00112, GGGP_HepGP_synthase, 1 hit |
InterProi | View protein in InterPro IPR038597, GGGP/HepGP_synthase_sf IPR008205, GGGP_HepGP_synthase IPR010946, GGGP_synth |
Pfami | View protein in Pfam PF01884, PcrB, 1 hit |
TIGRFAMsi | TIGR01769, GGGP, 1 hit TIGR01768, GGGP-family, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9HJH3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMTVLEDMLR KTRNGKVHMT LIDPGAKPPQ ECARIAEEAE MAGTDFIMVG
60 70 80 90 100
GSTDIDSRAM DEAISAIKAK TDLKVIIFPG SSLMISPKAD AIFFMSLLNS
110 120 130 140 150
GSLEYVVGHQ VKAAIPLSAM KIEKIPMAYL VFDPGMTVGR VGKAHLIPRD
160 170 180 190 200
DEKTALSYAL AAQYFGFRLV YFEAGSGSPY HVGENVVRRV KQELDIPVIV
210 220 230 240 250
GGGIRTPEAA KALAQAGADM IVTGTIAERS VNVYEALHPI VESIKEVGIS
KIQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL445066 Genomic DNA Translation: CAC12124.1 |
Genome annotation databases
EnsemblBacteriai | CAC12124; CAC12124; CAC12124 |
KEGGi | tac:Ta0995 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL445066 Genomic DNA Translation: CAC12124.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6JO3 | X-ray | 2.35 | A/B | 1-253 | [»] | |
AlphaFoldDBi | Q9HJH3 | |||||
SMRi | Q9HJH3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 273075.Ta0995 |
Genome annotation databases
EnsemblBacteriai | CAC12124; CAC12124; CAC12124 |
KEGGi | tac:Ta0995 |
Phylogenomic databases
eggNOGi | arCOG01085, Archaea |
HOGENOMi | CLU_068610_0_0_2 |
OMAi | ADAIFFM |
Enzyme and pathway databases
UniPathwayi | UPA00940 |
SABIO-RKi | Q9HJH3 |
Family and domain databases
CDDi | cd02812, PcrB_like, 1 hit |
Gene3Di | 3.20.20.390, 1 hit |
HAMAPi | MF_00112, GGGP_HepGP_synthase, 1 hit |
InterProi | View protein in InterPro IPR038597, GGGP/HepGP_synthase_sf IPR008205, GGGP_HepGP_synthase IPR010946, GGGP_synth |
Pfami | View protein in Pfam PF01884, PcrB, 1 hit |
TIGRFAMsi | TIGR01769, GGGP, 1 hit TIGR01768, GGGP-family, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GGGPS_THEAC | |
Accessioni | Q9HJH3Primary (citable) accession number: Q9HJH3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | March 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families