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Protein

DNA ligase

Gene

lig

Organism
Thermococcus fumicolans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. Shows high activity with either ATP or NAD+.1 Publication

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.UniRule annotation1 Publication
NAD+ + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + beta-nicotinamide D-nucleotide.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation1 Publication

Kineticsi

  1. KM=1160 µM for ATP1 Publication
  2. KM=690 µM for NAD+1 Publication

    pH dependencei

    Optimum pH is 7.0.1 Publication

    Temperature dependencei

    Optimum temperature is 65 degrees Celsius. Loses half of its activity in 15 min at 90 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei247ATPUniRule annotation1
    Active sitei249N6-AMP-lysine intermediateUniRule annotation1
    Binding sitei254ATPUniRule annotation1
    Binding sitei269ATPUniRule annotation1
    Binding sitei299ATPUniRule annotation1
    Binding sitei339ATPUniRule annotation1
    Binding sitei414ATPUniRule annotation1
    Binding sitei420ATPUniRule annotation1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionLigase
    Biological processCell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication
    LigandATP-binding, Magnesium, Metal-binding, NAD, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi6.5.1.6 6299

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA ligase1 PublicationUniRule annotation (EC:6.5.1.6UniRule annotation1 Publication)
    Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP/NAD(+)]UniRule annotationCurated
    Gene namesi
    Name:ligUniRule annotation
    OrganismiThermococcus fumicolans
    Taxonomic identifieri46540 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000596211 – 559DNA ligaseAdd BLAST559

    Proteomic databases

    PRIDEiQ9HH07

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HH07
    SMRiQ9HH07
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATP-dependent DNA ligase family.UniRule annotationCurated

    Phylogenomic databases

    KOiK10747

    Family and domain databases

    Gene3Di1.10.3260.10, 1 hit
    HAMAPiMF_00407 DNA_ligase, 1 hit
    InterProiView protein in InterPro
    IPR022865 DNA_ligae_ATP-dep_bac/arc
    IPR000977 DNA_ligase_ATP-dep
    IPR012309 DNA_ligase_ATP-dep_C
    IPR012310 DNA_ligase_ATP-dep_cent
    IPR016059 DNA_ligase_ATP-dep_CS
    IPR012308 DNA_ligase_ATP-dep_N
    IPR036599 DNA_ligase_N_sf
    IPR012340 NA-bd_OB-fold
    PfamiView protein in Pfam
    PF04679 DNA_ligase_A_C, 1 hit
    PF01068 DNA_ligase_A_M, 1 hit
    PF04675 DNA_ligase_A_N, 1 hit
    SUPFAMiSSF117018 SSF117018, 1 hit
    SSF50249 SSF50249, 1 hit
    TIGRFAMsiTIGR00574 dnl1, 1 hit
    PROSITEiView protein in PROSITE
    PS00697 DNA_LIGASE_A1, 1 hit
    PS00333 DNA_LIGASE_A2, 1 hit
    PS50160 DNA_LIGASE_A3, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9HH07-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKYSELAGLY RRLEKTTLKT LKTRFVADFL KNVPDELLEI VPYLILGKVF
    60 70 80 90 100
    PDWDERELGV GEKLLIKAVS IATGVPEGEI ENSIKDTGDL GESIALAVKK
    110 120 130 140 150
    KKQKSFFSQP LTIKRVYDTF VKVAESQGEG SQDRKMKYLA NLFMDAQPEE
    160 170 180 190 200
    AKYIARTVLG TMRTGVAEGI LRDAIAEAFK VKAELVERAY MLTSDFGYVT
    210 220 230 240 250
    KVAKLEGNEG LSKVRIQVGK PVRPMLAQNA ASVKDALLEM GGEAAFEIKY
    260 270 280 290 300
    DGARVQVHKD GDRVVIYSRR LENVTRSIPE IVEAVRSQLR PEKAIVEGEL
    310 320 330 340 350
    VAVGDGGKPR PFQYVLRRFR RKYNIEEMIE RIPLELNLFD VLYVDGESLV
    360 370 380 390 400
    DTPFMERRKR LEEAVEESER IKLAQQLVTK KAEEAEEFYR RALELGHEGL
    410 420 430 440 450
    MAKRLDSVYE PGNRGKKWLK IKPTMEDLDL VIIGAEWGEG RRAHLLGSFL
    460 470 480 490 500
    VAAYDQHRGE FVPVGKVGSG FTDEDLAEFT KMLKPLIVRE EGKYVEIEPR
    510 520 530 540 550
    VVIQVTYQEI QKSPKYESGF ALRFPRYVAL REDKSPEEAD TIERISELYG

    LQERFKAKR
    Length:559
    Mass (Da):63,598
    Last modified:March 1, 2001 - v1
    Checksum:i173DCFF6FC0110BB
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ133713 Genomic DNA Translation: CAC21199.1

    Genome annotation databases

    KEGGiag:CAC21199

    Similar proteinsi

    Entry informationi

    Entry nameiDNLI_THEFM
    AccessioniPrimary (citable) accession number: Q9HH07
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: March 1, 2001
    Last modified: May 23, 2018
    This is version 81 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

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