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Protein

Uracil phosphoribosyltransferase 2

Gene

SPAC1399.04c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.By similarity

Catalytic activityi

UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. The magnesium is bound as Mg-PRPP.By similarity

Activity regulationi

Allosterically activated by GTP.By similarity

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from uracil.
Proteins known to be involved in this subpathway in this organism are:
  1. Uracil phosphoribosyltransferase 2 (SPAC1399.04c), Uracil phosphoribosyltransferase 1 (SPAC1B3.01c), Putative uracil phosphoribosyltransferase urg2 (urg2)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from uracil, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei875-phospho-alpha-D-ribose 1-diphosphateBy similarity1
Binding sitei1135-phospho-alpha-D-ribose 1-diphosphateBy similarity1
Binding sitei134GTPBy similarity1
Binding sitei1405-phospho-alpha-D-ribose 1-diphosphateBy similarity1
Binding sitei204Ribose-5-phosphateBy similarity1
Binding sitei205Uracil; via amide nitrogenBy similarity1
Binding sitei2115-phospho-alpha-D-ribose 1-diphosphateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 80GTPBy similarity4

GO - Molecular functioni

  • GTP binding Source: UniProtKB-KW
  • uracil phosphoribosyltransferase activity Source: PomBase
  • uridine kinase activity Source: GO_Central

GO - Biological processi

  • pyrimidine-containing compound salvage Source: PomBase
  • UMP salvage Source: UniProtKB-UniPathway

Keywordsi

Molecular functionAllosteric enzyme, Glycosyltransferase, Transferase
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00574;UER00636

Names & Taxonomyi

Protein namesi
Recommended name:
Uracil phosphoribosyltransferase 2 (EC:2.4.2.9)
Short name:
UPRTase 2
Alternative name(s):
UMP pyrophosphorylase 2
Gene namesi
ORF Names:SPAC1399.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1399.04c
PomBaseiSPAC1399.04c

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001207871 – 220Uracil phosphoribosyltransferase 2Add BLAST220

Proteomic databases

MaxQBiQ9HE15
PaxDbiQ9HE15
PRIDEiQ9HE15

Interactioni

Protein-protein interaction databases

BioGridi279068, 29 interactors
STRINGi4896.SPAC1399.04c.1

Structurei

3D structure databases

ProteinModelPortaliQ9HE15
SMRiQ9HE15
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni140 – 1485-phospho-alpha-D-ribose 1-diphosphate bindingBy similarity9
Regioni210 – 212Uracil bindingBy similarity3

Sequence similaritiesi

Belongs to the UPRTase family.Curated

Phylogenomic databases

HOGENOMiHOG000262755
InParanoidiQ9HE15
KOiK00761
OMAiVVYAKFP
OrthoDBiEOG092C4GPE
PhylomeDBiQ9HE15

Family and domain databases

CDDicd06223 PRTases_typeI, 1 hit
InterProiView protein in InterPro
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
SUPFAMiSSF53271 SSF53271, 1 hit

Sequencei

Sequence statusi: Complete.

Q9HE15-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSIPLEQPEN VVVLRQTMYL LSLMTILRDQ QTGHSEFVRT ANLIINMLMQ
60 70 80 90 100
EALSALPYKK CLIKTSSGGT YTGVQPARDI CGVSILRAGE SMEYGLAAAC
110 120 130 140 150
NYSVPVGKLL VQRDETTFEA KLMFCKLPKD AQDRLVLLLD PLLATGNSVI
160 170 180 190 200
LAIQTLINKG IPEENIVFVN LIACNEGITN VFAKFPKLRM VTASIDPELN
210 220
ANKYVVPGCG DFGDRYFGTC
Length:220
Mass (Da):24,155
Last modified:March 1, 2001 - v1
Checksum:iAE1756C4DD11C37C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAC19743.1
RefSeqiNP_593510.1, NM_001018944.1

Genome annotation databases

EnsemblFungiiSPAC1399.04c.1; SPAC1399.04c.1:pep; SPAC1399.04c
GeneIDi2542614
KEGGispo:SPAC1399.04c

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAC19743.1
RefSeqiNP_593510.1, NM_001018944.1

3D structure databases

ProteinModelPortaliQ9HE15
SMRiQ9HE15
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279068, 29 interactors
STRINGi4896.SPAC1399.04c.1

Proteomic databases

MaxQBiQ9HE15
PaxDbiQ9HE15
PRIDEiQ9HE15

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC1399.04c.1; SPAC1399.04c.1:pep; SPAC1399.04c
GeneIDi2542614
KEGGispo:SPAC1399.04c

Organism-specific databases

EuPathDBiFungiDB:SPAC1399.04c
PomBaseiSPAC1399.04c

Phylogenomic databases

HOGENOMiHOG000262755
InParanoidiQ9HE15
KOiK00761
OMAiVVYAKFP
OrthoDBiEOG092C4GPE
PhylomeDBiQ9HE15

Enzyme and pathway databases

UniPathwayi
UPA00574;UER00636

Miscellaneous databases

PROiPR:Q9HE15

Family and domain databases

CDDicd06223 PRTases_typeI, 1 hit
InterProiView protein in InterPro
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
SUPFAMiSSF53271 SSF53271, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUPP2_SCHPO
AccessioniPrimary (citable) accession number: Q9HE15
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: March 1, 2001
Last modified: November 7, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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