UniProtKB - Q9HDY0 (YPT71_SCHPO)
Protein
Ypt/Rab-type GTPase ypt71
Gene
ypt71
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Functioni
Ypt/Rab-type GTPases are key regulators of membrane trafficking and intracellular vesicular transport. They act as molecular switches that convert between GTP-bound and GDP-bound states, and regulate virtually all steps of membrane traffic from the formation of the transport vesicle at the donor membrane to its fusion at the target membrane. In the GDP-bound state, Ypt proteins are predominantly cytosolic, solubilized through the interaction with a GDP dissociation inhibitor (GDI). In the GTP-bound state, the proteins are membrane bound and interact with specific effector proteins that select cargo, promote vesicle movement, or verify the correct site of fusion (By similarity). Act antagonistically to ypt7 in regulating vacuolar morphology, promoting vacuolar fission (PubMed:19453973).By similarity1 Publication
Activity regulationi
Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 66 | GTP; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 17 – 23 | GTPBy similarity | 7 | |
Nucleotide bindingi | 33 – 40 | GTPBy similarity | 8 | |
Nucleotide bindingi | 124 – 127 | GTPBy similarity | 4 | |
Nucleotide bindingi | 158 – 160 | GTPBy similarity | 3 |
GO - Molecular functioni
- GTPase activity Source: PomBase
- GTP binding Source: UniProtKB-KW
GO - Biological processi
- endocytosis Source: PomBase
- Golgi to vacuole transport Source: PomBase
- intracellular protein transport Source: PomBase
- negative regulation of vacuole fusion, non-autophagic Source: PomBase
- regulation of vacuole fusion, non-autophagic Source: GO_Central
- small GTPase mediated signal transduction Source: PomBase
Keywordsi
Biological process | Protein transport, Transport |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SPO-6798695, Neutrophil degranulation R-SPO-8854214, TBC/RABGAPs R-SPO-8873719, RAB geranylgeranylation |
Names & Taxonomyi
Protein namesi | Recommended name: Ypt/Rab-type GTPase ypt71 |
Gene namesi | Name:ypt71 ORF Names:SPAPB1A10.10c |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:SPAPB1A10.10c |
PomBasei | SPAPB1A10.10c, ypt71 |
Subcellular locationi
Vacuole
- Vacuole membrane 1 Publication
Cytosol
- cytosol Source: PomBase
Endosome
- late endosome Source: GO_Central
Lysosome
- lysosome Source: GO_Central
Nucleus
- nucleus Source: PomBase
Vacuole
- fungal-type vacuole membrane Source: PomBase
- lysosome Source: GO_Central
Other locations
- phagocytic vesicle Source: GO_Central
Keywords - Cellular componenti
Membrane, VacuolePathology & Biotechi
Disruption phenotypei
Contains large vacuoles.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000121314 | 1 – 208 | Ypt/Rab-type GTPase ypt71Add BLAST | 208 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 206 | S-geranylgeranyl cysteineBy similarity | 1 | |
Modified residuei | 208 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 208 | S-geranylgeranyl cysteineBy similarity | 1 |
Keywords - PTMi
Lipoprotein, Methylation, PrenylationProteomic databases
MaxQBi | Q9HDY0 |
PaxDbi | Q9HDY0 |
PRIDEi | Q9HDY0 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 279833, 7 interactors |
STRINGi | 4896.SPAPB1A10.10c.1 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 37 – 45 | Effector regionBy similarity | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0394, Eukaryota |
HOGENOMi | CLU_041217_10_6_1 |
InParanoidi | Q9HDY0 |
OMAi | CENNGNI |
PhylomeDBi | Q9HDY0 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9HDY0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAQKRVFLK VVILGDSGVG KTCLMNQFVN QKFSREYKAT IGADFLTKDV
60 70 80 90 100
VVDDKLVTLQ LWDTAGQERF QSLGMAFYRG ADCCVIVYNV NNSKSFDSVE
110 120 130 140 150
NWRQEFLYQT SQDECAFPFI IVGNQIDKDA SKRAVSLHRA LDYCKSKHGS
160 170 180 190 200
NMIHFEASAK ENTNVTDLFE TVSRLALENE SSRDDFVNDF SEPLLLSKPL
NNTSSCNC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329670 Genomic DNA Translation: CAC21483.1 |
RefSeqi | NP_593524.1, NM_001018958.2 |
Genome annotation databases
EnsemblFungii | SPAPB1A10.10c.1; SPAPB1A10.10c.1:pep; SPAPB1A10.10c |
GeneIDi | 2543411 |
KEGGi | spo:SPAPB1A10.10c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329670 Genomic DNA Translation: CAC21483.1 |
RefSeqi | NP_593524.1, NM_001018958.2 |
3D structure databases
SMRi | Q9HDY0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 279833, 7 interactors |
STRINGi | 4896.SPAPB1A10.10c.1 |
Proteomic databases
MaxQBi | Q9HDY0 |
PaxDbi | Q9HDY0 |
PRIDEi | Q9HDY0 |
Genome annotation databases
EnsemblFungii | SPAPB1A10.10c.1; SPAPB1A10.10c.1:pep; SPAPB1A10.10c |
GeneIDi | 2543411 |
KEGGi | spo:SPAPB1A10.10c |
Organism-specific databases
EuPathDBi | FungiDB:SPAPB1A10.10c |
PomBasei | SPAPB1A10.10c, ypt71 |
Phylogenomic databases
eggNOGi | KOG0394, Eukaryota |
HOGENOMi | CLU_041217_10_6_1 |
InParanoidi | Q9HDY0 |
OMAi | CENNGNI |
PhylomeDBi | Q9HDY0 |
Enzyme and pathway databases
Reactomei | R-SPO-6798695, Neutrophil degranulation R-SPO-8854214, TBC/RABGAPs R-SPO-8873719, RAB geranylgeranylation |
Miscellaneous databases
PROi | PR:Q9HDY0 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YPT71_SCHPO | |
Accessioni | Q9HDY0Primary (citable) accession number: Q9HDY0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 24, 2006 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - SIMILARITY comments
Index of protein domains and families