UniProtKB - Q9HCU5 (PREB_HUMAN)
Prolactin regulatory element-binding protein
PREB
Functioni
Guanine nucleotide exchange factor that specifically activates the small GTPase SAR1B. Mediates the recruitment of SAR1B and other COPII coat components to endoplasmic reticulum membranes and is therefore required for the formation of COPII transport vesicles from the ER.
By similarityWas first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription.
By similarityGO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- GTPase activator activity Source: GO_Central
- GTPase binding Source: Ensembl
- guanyl-nucleotide exchange factor activity Source: UniProtKB
GO - Biological processi
- COPII vesicle coating Source: UniProtKB
- endoplasmic reticulum to Golgi vesicle-mediated transport Source: UniProtKB
- protein exit from endoplasmic reticulum Source: UniProtKB
- protein secretion Source: GO_Central
- regulation of COPII vesicle coating Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | ER-Golgi transport, Protein transport, Transcription, Transcription regulation, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q9HCU5 |
Reactomei | R-HSA-204005, COPII-mediated vesicle transport R-HSA-381038, XBP1(S) activates chaperone genes R-HSA-5694530, Cargo concentration in the ER |
SignaLinki | Q9HCU5 |
Protein family/group databases
TCDBi | 8.A.92.1.10, the g-protein AlphaBetaGama complex (gpc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Prolactin regulatory element-binding proteinAlternative name(s): Mammalian guanine nucleotide exchange factor mSec12 |
Gene namesi | Name:PREB Synonyms:SEC12 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9356, PREB |
MIMi | 606395, gene |
neXtProti | NX_Q9HCU5 |
VEuPathDBi | HostDB:ENSG00000138073 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass membrane protein By similarity
Note: Concentrates at endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER).2 Publications
Endoplasmic reticulum
- endoplasmic reticulum exit site Source: UniProtKB
- endoplasmic reticulum membrane Source: Reactome
- integral component of endoplasmic reticulum membrane Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB
Other locations
- membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 388 | CytoplasmicSequence analysisAdd BLAST | 388 | |
Transmembranei | 389 – 409 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 410 – 417 | LumenalSequence analysis | 8 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 10113 |
OpenTargetsi | ENSG00000138073 |
PharmGKBi | PA33727 |
Miscellaneous databases
Pharosi | Q9HCU5, Tbio |
Genetic variation databases
BioMutai | PREB |
DMDMi | 55977881 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051154 | 1 – 417 | Prolactin regulatory element-binding proteinAdd BLAST | 417 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 10 | 3'-nitrotyrosineBy similarity | 1 |
Keywords - PTMi
NitrationProteomic databases
EPDi | Q9HCU5 |
jPOSTi | Q9HCU5 |
MassIVEi | Q9HCU5 |
MaxQBi | Q9HCU5 |
PaxDbi | Q9HCU5 |
PeptideAtlasi | Q9HCU5 |
PRIDEi | Q9HCU5 |
ProteomicsDBi | 81801 |
PTM databases
iPTMneti | Q9HCU5 |
PhosphoSitePlusi | Q9HCU5 |
SwissPalmi | Q9HCU5 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000138073, Expressed in right lobe of liver and 231 other tissues |
ExpressionAtlasi | Q9HCU5, baseline and differential |
Genevisiblei | Q9HCU5, HS |
Organism-specific databases
HPAi | ENSG00000138073, Low tissue specificity |
Interactioni
Subunit structurei
GO - Molecular functioni
- GTPase binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 115419, 133 interactors |
CORUMi | Q9HCU5 |
IntActi | Q9HCU5, 31 interactors |
MINTi | Q9HCU5 |
STRINGi | 9606.ENSP00000260643 |
Miscellaneous databases
RNActi | Q9HCU5, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9HCU5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 4 – 50 | WD 1Add BLAST | 47 | |
Repeati | 58 – 97 | WD 2Add BLAST | 40 | |
Repeati | 127 – 183 | WD 3Add BLAST | 57 | |
Repeati | 187 – 224 | WD 4Add BLAST | 38 | |
Repeati | 230 – 287 | WD 5Add BLAST | 58 | |
Repeati | 291 – 329 | WD 6Add BLAST | 39 | |
Repeati | 334 – 385 | WD 7Add BLAST | 52 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 101 – 130 | DisorderedSequence analysisAdd BLAST | 30 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 109 – 130 | Basic and acidic residuesSequence analysisAdd BLAST | 22 |
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helix, WD repeatPhylogenomic databases
eggNOGi | KOG0771, Eukaryota |
GeneTreei | ENSGT00390000018031 |
HOGENOMi | CLU_054579_1_0_1 |
InParanoidi | Q9HCU5 |
OMAi | AHCQMYY |
OrthoDBi | 828247at2759 |
PhylomeDBi | Q9HCU5 |
TreeFami | TF314383 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR011047, Quinoprotein_ADH-like_supfam IPR045260, Sec12-like IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat |
PANTHERi | PTHR23284, PTHR23284, 1 hit |
Pfami | View protein in Pfam PF00400, WD40, 1 hit |
SMARTi | View protein in SMART SM00320, WD40, 4 hits |
SUPFAMi | SSF50998, SSF50998, 1 hit |
PROSITEi | View protein in PROSITE PS50082, WD_REPEATS_2, 1 hit PS50294, WD_REPEATS_REGION, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MGRRRAPELY RAPFPLYALQ VDPSTGLLIA AGGGGAAKTG IKNGVHFLQL
60 70 80 90 100
ELINGRLSAS LLHSHDTETR ATMNLALAGD ILAAGQDAHC QLLRFQAHQQ
110 120 130 140 150
QGNKAEKAGS KEQGPRQRKG AAPAEKKCGA ETQHEGLELR VENLQAVQTD
160 170 180 190 200
FSSDPLQKVV CFNHDNTLLA TGGTDGYVRV WKVPSLEKVL EFKAHEGEIE
210 220 230 240 250
DLALGPDGKL VTVGRDLKAS VWQKDQLVTQ LHWQENGPTF SSTPYRYQAC
260 270 280 290 300
RFGQVPDQPA GLRLFTVQIP HKRLRQPPPC YLTAWDGSNF LPLRTKSCGH
310 320 330 340 350
EVVSCLDVSE SGTFLGLGTV TGSVAIYIAF SLQCLYYVRE AHGIVVTDVA
360 370 380 390 400
FLPEKGRGPE LLGSHETALF SVAVDSRCQL HLLPSRRSVP VWLLLLLCVG
410
LIIVTILLLQ SAFPGFL
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB5MC98 | B5MC98_HUMAN | Prolactin regulatory element-bindin... | PREB | 359 | Annotation score: | ||
H7C2N7 | H7C2N7_HUMAN | Prolactin regulatory element-bindin... | PREB | 106 | Annotation score: | ||
H7C0W3 | H7C0W3_HUMAN | Prolactin regulatory element-bindin... | PREB | 176 | Annotation score: | ||
F8WCS5 | F8WCS5_HUMAN | Prolactin regulatory element-bindin... | PREB | 50 | Annotation score: | ||
H7BZX0 | H7BZX0_HUMAN | Prolactin regulatory element-bindin... | PREB | 22 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 19 | L → F in AAG01692 (PubMed:10920239).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF226684 mRNA Translation: AAG01692.1 AF203687 mRNA Translation: AAF19192.1 AF227166 Genomic DNA Translation: AAF74572.1 AK023064 mRNA Translation: BAB14385.1 AC013403 Genomic DNA Translation: AAX93170.1 CH471053 Genomic DNA Translation: EAX00627.1 BC002765 mRNA Translation: AAH02765.1 BC012890 mRNA Translation: AAH12890.1 BC016472 mRNA Translation: AAH16472.1 BC041032 mRNA Translation: AAH41032.1 |
CCDSi | CCDS1738.1 |
RefSeqi | NP_001317414.1, NM_001330485.1 NP_001317415.1, NM_001330486.1 NP_037520.1, NM_013388.5 |
Genome annotation databases
Ensembli | ENST00000260643; ENSP00000260643; ENSG00000138073 |
GeneIDi | 10113 |
KEGGi | hsa:10113 |
MANE-Selecti | ENST00000260643.7; ENSP00000260643.2; NM_013388.6; NP_037520.1 |
UCSCi | uc002rix.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF226684 mRNA Translation: AAG01692.1 AF203687 mRNA Translation: AAF19192.1 AF227166 Genomic DNA Translation: AAF74572.1 AK023064 mRNA Translation: BAB14385.1 AC013403 Genomic DNA Translation: AAX93170.1 CH471053 Genomic DNA Translation: EAX00627.1 BC002765 mRNA Translation: AAH02765.1 BC012890 mRNA Translation: AAH12890.1 BC016472 mRNA Translation: AAH16472.1 BC041032 mRNA Translation: AAH41032.1 |
CCDSi | CCDS1738.1 |
RefSeqi | NP_001317414.1, NM_001330485.1 NP_001317415.1, NM_001330486.1 NP_037520.1, NM_013388.5 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5TF2 | X-ray | 2.80 | A | 1-389 | [»] | |
SMRi | Q9HCU5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115419, 133 interactors |
CORUMi | Q9HCU5 |
IntActi | Q9HCU5, 31 interactors |
MINTi | Q9HCU5 |
STRINGi | 9606.ENSP00000260643 |
Protein family/group databases
TCDBi | 8.A.92.1.10, the g-protein AlphaBetaGama complex (gpc) family |
PTM databases
iPTMneti | Q9HCU5 |
PhosphoSitePlusi | Q9HCU5 |
SwissPalmi | Q9HCU5 |
Genetic variation databases
BioMutai | PREB |
DMDMi | 55977881 |
Proteomic databases
EPDi | Q9HCU5 |
jPOSTi | Q9HCU5 |
MassIVEi | Q9HCU5 |
MaxQBi | Q9HCU5 |
PaxDbi | Q9HCU5 |
PeptideAtlasi | Q9HCU5 |
PRIDEi | Q9HCU5 |
ProteomicsDBi | 81801 |
Protocols and materials databases
Antibodypediai | 3012, 115 antibodies from 27 providers |
DNASUi | 10113 |
Genome annotation databases
Ensembli | ENST00000260643; ENSP00000260643; ENSG00000138073 |
GeneIDi | 10113 |
KEGGi | hsa:10113 |
MANE-Selecti | ENST00000260643.7; ENSP00000260643.2; NM_013388.6; NP_037520.1 |
UCSCi | uc002rix.3, human |
Organism-specific databases
CTDi | 10113 |
DisGeNETi | 10113 |
GeneCardsi | PREB |
HGNCi | HGNC:9356, PREB |
HPAi | ENSG00000138073, Low tissue specificity |
MIMi | 606395, gene |
neXtProti | NX_Q9HCU5 |
OpenTargetsi | ENSG00000138073 |
PharmGKBi | PA33727 |
VEuPathDBi | HostDB:ENSG00000138073 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0771, Eukaryota |
GeneTreei | ENSGT00390000018031 |
HOGENOMi | CLU_054579_1_0_1 |
InParanoidi | Q9HCU5 |
OMAi | AHCQMYY |
OrthoDBi | 828247at2759 |
PhylomeDBi | Q9HCU5 |
TreeFami | TF314383 |
Enzyme and pathway databases
PathwayCommonsi | Q9HCU5 |
Reactomei | R-HSA-204005, COPII-mediated vesicle transport R-HSA-381038, XBP1(S) activates chaperone genes R-HSA-5694530, Cargo concentration in the ER |
SignaLinki | Q9HCU5 |
Miscellaneous databases
BioGRID-ORCSi | 10113, 718 hits in 1048 CRISPR screens |
ChiTaRSi | PREB, human |
GeneWikii | PREB |
GenomeRNAii | 10113 |
Pharosi | Q9HCU5, Tbio |
PROi | PR:Q9HCU5 |
RNActi | Q9HCU5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000138073, Expressed in right lobe of liver and 231 other tissues |
ExpressionAtlasi | Q9HCU5, baseline and differential |
Genevisiblei | Q9HCU5, HS |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR011047, Quinoprotein_ADH-like_supfam IPR045260, Sec12-like IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat |
PANTHERi | PTHR23284, PTHR23284, 1 hit |
Pfami | View protein in Pfam PF00400, WD40, 1 hit |
SMARTi | View protein in SMART SM00320, WD40, 4 hits |
SUPFAMi | SSF50998, SSF50998, 1 hit |
PROSITEi | View protein in PROSITE PS50082, WD_REPEATS_2, 1 hit PS50294, WD_REPEATS_REGION, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PREB_HUMAN | |
Accessioni | Q9HCU5Primary (citable) accession number: Q9HCU5 Secondary accession number(s): Q53SZ8, Q9UH94 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 24, 2001 |
Last sequence update: | November 23, 2004 | |
Last modified: | February 23, 2022 | |
This is version 171 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references