UniProtKB - Q9HCQ5 (GALT9_HUMAN)
Polypeptide N-acetylgalactosaminyltransferase 9
GALNT9
Functioni
Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Does not glycosylate apomucin or SDC3.
2 PublicationsCatalytic activityi
- L-seryl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-seryl-[protein] + H+ + UDP1 PublicationEC:2.4.1.411 Publication
- L-threonyl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-threonyl-[protein] + H+ + UDP1 PublicationEC:2.4.1.411 Publication
Cofactori
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 191 | SubstrateBy similarity | 1 | |
Binding sitei | 222 | SubstrateBy similarity | 1 | |
Metal bindingi | 245 | ManganeseBy similarity | 1 | |
Metal bindingi | 247 | ManganeseBy similarity | 1 | |
Metal bindingi | 377 | ManganeseBy similarity | 1 | |
Binding sitei | 380 | SubstrateBy similarity | 1 | |
Binding sitei | 385 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- carbohydrate binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- polypeptide N-acetylgalactosaminyltransferase activity Source: Reactome
GO - Biological processi
- O-glycan processing Source: Reactome
- protein O-linked glycosylation Source: UniProtKB
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Ligand | Lectin, Manganese, Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.4.1.41, 2681 |
PathwayCommonsi | Q9HCQ5 |
Reactomei | R-HSA-913709, O-linked glycosylation of mucins |
SignaLinki | Q9HCQ5 |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
Names & Taxonomyi
Protein namesi | Recommended name: Polypeptide N-acetylgalactosaminyltransferase 9 (EC:2.4.1.411 Publication)Alternative name(s): Polypeptide GalNAc transferase 9 Short name: GalNAc-T9 Short name: pp-GaNTase 9 Protein-UDP acetylgalactosaminyltransferase 9 UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 9 |
Gene namesi | Name:GALNT9 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:4131, GALNT9 |
MIMi | 606251, gene |
neXtProti | NX_Q9HCQ5 |
VEuPathDBi | HostDB:ENSG00000182870 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity
Golgi apparatus
- Golgi apparatus Source: GO_Central
- Golgi membrane Source: Reactome
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 6 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 7 – 29 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 23 | |
Topological domaini | 30 – 603 | LumenalSequence analysisAdd BLAST | 574 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 50614 |
OpenTargetsi | ENSG00000182870 |
PharmGKBi | PA28544 |
Miscellaneous databases
Pharosi | Q9HCQ5, Tbio |
Genetic variation databases
BioMutai | GALNT9 |
DMDMi | 143811393 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000059120 | 1 – 603 | Polypeptide N-acetylgalactosaminyltransferase 9Add BLAST | 603 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 141 ↔ 372 | PROSITE-ProRule annotation | ||
Disulfide bondi | 363 ↔ 442 | PROSITE-ProRule annotation | ||
Glycosylationi | 460 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 477 ↔ 493 | PROSITE-ProRule annotation | ||
Disulfide bondi | 525 ↔ 540 | PROSITE-ProRule annotation | ||
Disulfide bondi | 567 ↔ 587 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MassIVEi | Q9HCQ5 |
PaxDbi | Q9HCQ5 |
PeptideAtlasi | Q9HCQ5 |
PRIDEi | Q9HCQ5 |
ProteomicsDBi | 81785 [Q9HCQ5-1] 81786 [Q9HCQ5-2] |
PTM databases
GlyGeni | Q9HCQ5, 1 site |
iPTMneti | Q9HCQ5 |
PhosphoSitePlusi | Q9HCQ5 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000182870, Expressed in right hemisphere of cerebellum and 132 other tissues |
ExpressionAtlasi | Q9HCQ5, baseline and differential |
Genevisiblei | Q9HCQ5, HS |
Organism-specific databases
HPAi | ENSG00000182870, Tissue enhanced (brain, thyroid gland) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 119094, 53 interactors |
IntActi | Q9HCQ5, 1 interactor |
STRINGi | 9606.ENSP00000380488 |
Miscellaneous databases
RNActi | Q9HCQ5, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 464 – 600 | Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST | 137 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 150 – 261 | Catalytic subdomain AAdd BLAST | 112 | |
Regioni | 318 – 380 | Catalytic subdomain BAdd BLAST | 63 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3736, Eukaryota |
GeneTreei | ENSGT00940000156599 |
HOGENOMi | CLU_013477_2_2_1 |
InParanoidi | Q9HCQ5 |
OMAi | QYVHRRY |
OrthoDBi | 273006at2759 |
PhylomeDBi | Q9HCQ5 |
TreeFami | TF313267 |
Family and domain databases
CDDi | cd02510, pp-GalNAc-T, 1 hit cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR045885, GalNAc-T IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAVARKIRTL LTVNILVFVG IVLFSVYCRL QGRSQELVRI VSGDRRVRSR
60 70 80 90 100
HAKVGTLGDR EAILQRLDHL EEVVYNQLNG LAKPIGLVEG PGGLGQGGLA
110 120 130 140 150
ATLRDDGQEA EGKYEEYGYN AQLSDRISLD RSIPDYRPRK CRQMSYAQDL
160 170 180 190 200
PQVSVVFIFV NEALSVILRS VHSVVNHTPS QLLKEVILVD DNSDNVELKF
210 220 230 240 250
NLDQYVNKRY PGLVKIVRNS RREGLIRARL QGWKAATAPV VGFFDAHVEF
260 270 280 290 300
NTGWAEPALS RIREDRRRIV LPAIDNIKYS TFEVQQYANA AHGYNWGLRC
310 320 330 340 350
MYIIPPQDWL DRGDESAPIR TPAMIGCSFV VDREYFGDIG LLDPGMEVYG
360 370 380 390 400
GENVELGMRV WQCGGSMEVL PCSRVAHIER TRKPYNNDID YYAKRNALRA
410 420 430 440 450
AEVWMDDFKS HVYMAWNIPM SNPGVDFGDV SERLALRQRL KCRSFKWYLE
460 470 480 490 500
NVYPEMRVYN NTLTYGEVRN SKASAYCLDQ GAEDGDRAIL YPCHGMSSQL
510 520 530 540 550
VRYSADGLLQ LGPLGSTAFL PDSKCLVDDG TGRMPTLKKC EDVARPTQRL
560 570 580 590 600
WDFTQSGPIV SRATGRCLEV EMSKDANFGL RLVVQRCSGQ KWMIRNWIKH
ARH
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketJ3KNN1 | J3KNN1_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT9 | 603 | Annotation score: | ||
H3BM10 | H3BM10_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT9 | 376 | Annotation score: | ||
F5H557 | F5H557_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT9 | 96 | Annotation score: | ||
F5H317 | F5H317_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT9 | 54 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 539 | K → R in BAB13699 (PubMed:10978536).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_011206 | 1 – 366 | Missing in isoform 2. 2 PublicationsAdd BLAST | 366 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB040672 mRNA Translation: BAB13699.2 AF458594 mRNA Translation: AAM49722.1 BC093817 mRNA Translation: AAH93817.2 BC093819 mRNA Translation: AAH93819.2 |
CCDSi | CCDS41866.1 [Q9HCQ5-2] CCDS81755.1 [Q9HCQ5-1] |
RefSeqi | NP_001116108.1, NM_001122636.1 NP_068580.2, NM_021808.3 [Q9HCQ5-2] |
Genome annotation databases
Ensembli | ENST00000397325.6; ENSP00000380488.2; ENSG00000182870.13 [Q9HCQ5-2] ENST00000541995.5; ENSP00000440544.1; ENSG00000182870.13 [Q9HCQ5-2] ENST00000671997.1; ENSP00000500654.1; ENSG00000288490.1 [Q9HCQ5-2] ENST00000673376.1; ENSP00000500593.1; ENSG00000288490.1 [Q9HCQ5-2] |
GeneIDi | 50614 |
KEGGi | hsa:50614 |
UCSCi | uc001uka.3, human [Q9HCQ5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - GTase Polypeptide N-acetylgalactosaminyltransferase 9 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB040672 mRNA Translation: BAB13699.2 AF458594 mRNA Translation: AAM49722.1 BC093817 mRNA Translation: AAH93817.2 BC093819 mRNA Translation: AAH93819.2 |
CCDSi | CCDS41866.1 [Q9HCQ5-2] CCDS81755.1 [Q9HCQ5-1] |
RefSeqi | NP_001116108.1, NM_001122636.1 NP_068580.2, NM_021808.3 [Q9HCQ5-2] |
3D structure databases
AlphaFoldDBi | Q9HCQ5 |
SMRi | Q9HCQ5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 119094, 53 interactors |
IntActi | Q9HCQ5, 1 interactor |
STRINGi | 9606.ENSP00000380488 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
PTM databases
GlyGeni | Q9HCQ5, 1 site |
iPTMneti | Q9HCQ5 |
PhosphoSitePlusi | Q9HCQ5 |
Genetic variation databases
BioMutai | GALNT9 |
DMDMi | 143811393 |
Proteomic databases
MassIVEi | Q9HCQ5 |
PaxDbi | Q9HCQ5 |
PeptideAtlasi | Q9HCQ5 |
PRIDEi | Q9HCQ5 |
ProteomicsDBi | 81785 [Q9HCQ5-1] 81786 [Q9HCQ5-2] |
Protocols and materials databases
Antibodypediai | 32061, 147 antibodies from 21 providers |
DNASUi | 50614 |
Genome annotation databases
Ensembli | ENST00000397325.6; ENSP00000380488.2; ENSG00000182870.13 [Q9HCQ5-2] ENST00000541995.5; ENSP00000440544.1; ENSG00000182870.13 [Q9HCQ5-2] ENST00000671997.1; ENSP00000500654.1; ENSG00000288490.1 [Q9HCQ5-2] ENST00000673376.1; ENSP00000500593.1; ENSG00000288490.1 [Q9HCQ5-2] |
GeneIDi | 50614 |
KEGGi | hsa:50614 |
UCSCi | uc001uka.3, human [Q9HCQ5-1] |
Organism-specific databases
CTDi | 50614 |
DisGeNETi | 50614 |
GeneCardsi | GALNT9 |
HGNCi | HGNC:4131, GALNT9 |
HPAi | ENSG00000182870, Tissue enhanced (brain, thyroid gland) |
MIMi | 606251, gene |
neXtProti | NX_Q9HCQ5 |
OpenTargetsi | ENSG00000182870 |
PharmGKBi | PA28544 |
VEuPathDBi | HostDB:ENSG00000182870 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3736, Eukaryota |
GeneTreei | ENSGT00940000156599 |
HOGENOMi | CLU_013477_2_2_1 |
InParanoidi | Q9HCQ5 |
OMAi | QYVHRRY |
OrthoDBi | 273006at2759 |
PhylomeDBi | Q9HCQ5 |
TreeFami | TF313267 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 2.4.1.41, 2681 |
PathwayCommonsi | Q9HCQ5 |
Reactomei | R-HSA-913709, O-linked glycosylation of mucins |
SignaLinki | Q9HCQ5 |
Miscellaneous databases
BioGRID-ORCSi | 50614, 14 hits in 1063 CRISPR screens |
ChiTaRSi | GALNT9, human |
GenomeRNAii | 50614 |
Pharosi | Q9HCQ5, Tbio |
PROi | PR:Q9HCQ5 |
RNActi | Q9HCQ5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000182870, Expressed in right hemisphere of cerebellum and 132 other tissues |
ExpressionAtlasi | Q9HCQ5, baseline and differential |
Genevisiblei | Q9HCQ5, HS |
Family and domain databases
CDDi | cd02510, pp-GalNAc-T, 1 hit cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR045885, GalNAc-T IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GALT9_HUMAN | |
Accessioni | Q9HCQ5Primary (citable) accession number: Q9HCQ5 Secondary accession number(s): Q52LR8, Q6NT54, Q8NFR1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | April 3, 2007 | |
Last modified: | May 25, 2022 | |
This is version 158 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families