UniProtKB - Q9HCM9 (TRI39_HUMAN)
Protein
E3 ubiquitin-protein ligase TRIM39
Gene
TRIM39
Organism
Homo sapiens (Human)
Status
Functioni
E3 ubiquitin-protein ligase (PubMed:22529100). May facilitate apoptosis by inhibiting APC/C-Cdh1-mediated poly-ubiquitination and subsequent proteasome-mediated degradation of the pro-apoptotic protein MOAP1 (PubMed:19100260, PubMed:22529100). Regulates the G1/S transition of the cell cycle and DNA damage-induced G2 arrest by stabilizing CDKN1A/p21 (PubMed:23213251). Positively regulates CDKN1A/p21 stability by competing with DTL for CDKN1A/p21 binding, therefore disrupting DCX(DTL) E3 ubiquitin ligase complex-mediated CDKN1A/p21 ubiquitination and degradation (PubMed:23213251).3 Publications
Regulates the G1/S transition of the cell cycle and DNA damage-induced G2 arrest by stabilizing CDKN1A/p21 (PubMed:23213251). Positively regulates CDKN1A/p21 stability by competing with DTL for CDKN1A/p21 binding, therefore disrupting DCX(DTL) E3 ubiquitin ligase complex-mediated CDKN1A/p21 ubiquitination and degradation (PubMed:23213251). Negatively regulates the canonical NF-kappa-B signaling pathway via stabilization of CACTIN in an ubiquitination-independent manner (PubMed:26363554).2 Publications
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 29 – 70 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 | |
Zinc fingeri | 102 – 143 | B box-typePROSITE-ProRule annotationAdd BLAST | 42 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- ubiquitin protein ligase activity Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- innate immune response Source: GO_Central
- mitotic G2 DNA damage checkpoint Source: UniProtKB
- negative regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
- negative regulation of ubiquitin-dependent protein catabolic process Source: BHF-UCL
- positive regulation of apoptotic signaling pathway Source: BHF-UCL
- protein stabilization Source: UniProtKB
- protein ubiquitination Source: GO_Central
- regulation of cell cycle G1/S phase transition Source: UniProtKB
- regulation of gene expression Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Apoptosis, Cell cycle, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9HCM9 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase TRIM39 (EC:2.3.2.27)Alternative name(s): RING finger protein 23 RING-type E3 ubiquitin transferase TRIM39Curated Testis-abundant finger protein Tripartite motif-containing protein 39 |
Gene namesi | Name:TRIM39 Synonyms:RNF23, TFP |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:10065, TRIM39 |
MIMi | 605700, gene |
neXtProti | NX_Q9HCM9 |
VEuPathDBi | HostDB:ENSG00000204599.14 |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Nucleus
- Nucleus 1 Publication
Cytosol
- cytosol 1 Publication
Note: Found predominantly in the cytosol. Partial shift from the cytosol to the mitochondria when colocalized with MOAP1. Colocalizes with CDKN1A in the nucleus.2 Publications
Nucleus
- Nucleus 1 Publication
Note: Colocalizes with CDKN1A in the nucleus.1 Publication
Cytosol
- cytosol Source: BHF-UCL
Mitochondrion
- mitochondrion Source: BHF-UCL
Nucleus
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, Mitochondrion, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 56658 |
OpenTargetsi | ENSG00000204599 |
PharmGKBi | PA35535 |
Miscellaneous databases
Pharosi | Q9HCM9, Tbio |
Genetic variation databases
BioMutai | TRIM39 |
DMDMi | 56405385 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056257 | 1 – 518 | E3 ubiquitin-protein ligase TRIM39Add BLAST | 518 |
Post-translational modificationi
Autoubiquitinated.1 Publication
Keywords - PTMi
Ubl conjugationProteomic databases
jPOSTi | Q9HCM9 |
MassIVEi | Q9HCM9 |
MaxQBi | Q9HCM9 |
PaxDbi | Q9HCM9 |
PeptideAtlasi | Q9HCM9 |
PRIDEi | Q9HCM9 |
ProteomicsDBi | 81768 [Q9HCM9-1] 81769 [Q9HCM9-2] |
PTM databases
iPTMneti | Q9HCM9 |
PhosphoSitePlusi | Q9HCM9 |
Expressioni
Tissue specificityi
Ubiquitous; highly expressed in brain, heart, kidney, liver, skeletal muscle, spleen and testis.1 Publication
Gene expression databases
Bgeei | ENSG00000204599, Expressed in testis and 205 other tissues |
ExpressionAtlasi | Q9HCM9, baseline and differential |
Genevisiblei | Q9HCM9, HS |
Organism-specific databases
HPAi | ENSG00000204599, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Q9HCM9
TRIM39 - isoform 2 [Q9HCM9-2]
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 121170, 101 interactors |
IntActi | Q9HCM9, 78 interactors |
MINTi | Q9HCM9 |
STRINGi | 9606.ENSP00000365844 |
Miscellaneous databases
RNActi | Q9HCM9, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q9HCM9 |
SMRi | Q9HCM9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9HCM9 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 319 – 514 | B30.2/SPRYPROSITE-ProRule annotationAdd BLAST | 196 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 268 – 337 | Interaction with CDKN1A1 PublicationAdd BLAST | 70 | |
Regioni | 389 – 518 | Interaction with CDKN1A1 PublicationAdd BLAST | 130 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 181 – 250 | Sequence analysisAdd BLAST | 70 |
Sequence similaritiesi
Belongs to the TRIM/RBCC family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 29 – 70 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 | |
Zinc fingeri | 102 – 143 | B box-typePROSITE-ProRule annotationAdd BLAST | 42 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00940000154126 |
HOGENOMi | CLU_013137_0_3_1 |
InParanoidi | Q9HCM9 |
OMAi | WAVGVCQ |
PhylomeDBi | Q9HCM9 |
TreeFami | TF342569 |
Family and domain databases
CDDi | cd00021, BBOX, 1 hit cd13745, SPRY_PRY_TRIM39, 1 hit |
Gene3Di | 2.60.120.920, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR003879, Butyrophylin_SPRY IPR013320, ConA-like_dom_sf IPR006574, PRY IPR035033, PRY/SPRY_TRIM39 IPR003877, SPRY_dom IPR000315, Znf_B-box IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF13765, PRY, 1 hit PF00622, SPRY, 1 hit PF00643, zf-B_box, 1 hit |
PRINTSi | PR01407, BUTYPHLNCDUF |
SMARTi | View protein in SMART SM00336, BBOX, 1 hit SM00589, PRY, 1 hit SM00184, RING, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9HCM9-1) [UniParc]FASTAAdd to basket
Also known as: TIM39alpha1 Publication
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAETSLLEAG ASAASTAAAL ENLQVEASCS VCLEYLKEPV IIECGHNFCK
60 70 80 90 100
ACITRWWEDL ERDFPCPVCR KTSRYRSLRP NRQLGSMVEI AKQLQAVKRK
110 120 130 140 150
IRDESLCPQH HEALSLFCYE DQEAVCLICA ISHTHRAHTV VPLDDATQEY
160 170 180 190 200
KEKLQKCLEP LEQKLQEITR CKSSEEKKPG ELKRLVESRR QQILREFEEL
210 220 230 240 250
HRRLDEEQQV LLSRLEEEEQ DILQRLRENA AHLGDKRRDL AHLAAEVEGK
260 270 280 290 300
CLQSGFEMLK DVKSTLEKNI PRKFGGSLST ICPRDHKALL GLVKEINRCE
310 320 330 340 350
KVKTMEVTSV SIELEKNFSN FPRQYFALRK ILKQLIADVT LDPETAHPNL
360 370 380 390 400
VLSEDRKSVK FVETRLRDLP DTPRRFTFYP CVLATEGFTS GRHYWEVEVG
410 420 430 440 450
DKTHWAVGVC RDSVSRKGEL TPLPETGYWR VRLWNGDKYA ATTTPFTPLH
460 470 480 490 500
IKVKPKRVGI FLDYEAGTLS FYNVTDRSHI YTFTDTFTEK LWPLFYPGIR
510
AGRKNAAPLT IRPPTDWE
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2AAZ6 | A2AAZ6_HUMAN | E3 ubiquitin-protein ligase TRIM39 | TRIM39 | 260 | Annotation score: | ||
H0Y735 | H0Y735_HUMAN | E3 ubiquitin-protein ligase TRIM39 | TRIM39 | 227 | Annotation score: | ||
A2AAZ5 | A2AAZ5_HUMAN | E3 ubiquitin-protein ligase TRIM39 | TRIM39 | 144 | Annotation score: | ||
A2AAZ4 | A2AAZ4_HUMAN | E3 ubiquitin-protein ligase TRIM39 | TRIM39 | 74 | Annotation score: | ||
A2AAZ3 | A2AAZ3_HUMAN | E3 ubiquitin-protein ligase TRIM39 | TRIM39 | 59 | Annotation score: | ||
A2AAZ2 | A2AAZ2_HUMAN | E3 ubiquitin-protein ligase TRIM39 | TRIM39 | 48 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 137 | A → P in BAB16374 (PubMed:11006080).Curated | 1 | |
Sequence conflicti | 419 | E → K in BAD13703 (Ref. 3) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_005755 | 269 – 298 | Missing in isoform 2. 2 PublicationsAdd BLAST | 30 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB046381 mRNA Translation: BAB16374.1 BT007370 mRNA Translation: AAP36034.1 AB110937 Genomic DNA Translation: BAD13703.1 AK292512 mRNA Translation: BAF85201.1 AB110938 Genomic DNA Translation: BAD13704.1 AB202089 Genomic DNA Translation: BAE78608.1 AL662832 Genomic DNA No translation available. AL662795 Genomic DNA No translation available. AL773535 Genomic DNA No translation available. BX248580 Genomic DNA No translation available. CR759928 Genomic DNA No translation available. BX927214 Genomic DNA No translation available. CR759281 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX03283.1 CH471081 Genomic DNA Translation: EAX03284.1 BC007661 mRNA Translation: AAH07661.1 BC034985 mRNA Translation: AAH34985.1 |
CCDSi | CCDS34377.1 [Q9HCM9-1] CCDS34378.1 [Q9HCM9-2] |
PIRi | JC7387 |
RefSeqi | NP_067076.2, NM_021253.3 [Q9HCM9-1] NP_742013.1, NM_172016.2 [Q9HCM9-2] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB046381 mRNA Translation: BAB16374.1 BT007370 mRNA Translation: AAP36034.1 AB110937 Genomic DNA Translation: BAD13703.1 AK292512 mRNA Translation: BAF85201.1 AB110938 Genomic DNA Translation: BAD13704.1 AB202089 Genomic DNA Translation: BAE78608.1 AL662832 Genomic DNA No translation available. AL662795 Genomic DNA No translation available. AL773535 Genomic DNA No translation available. BX248580 Genomic DNA No translation available. CR759928 Genomic DNA No translation available. BX927214 Genomic DNA No translation available. CR759281 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX03283.1 CH471081 Genomic DNA Translation: EAX03284.1 BC007661 mRNA Translation: AAH07661.1 BC034985 mRNA Translation: AAH34985.1 |
CCDSi | CCDS34377.1 [Q9HCM9-1] CCDS34378.1 [Q9HCM9-2] |
PIRi | JC7387 |
RefSeqi | NP_067076.2, NM_021253.3 [Q9HCM9-1] NP_742013.1, NM_172016.2 [Q9HCM9-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2DID | NMR | - | A | 104-143 | [»] | |
2DIF | NMR | - | A | 104-143 | [»] | |
2ECJ | NMR | - | A | 19-69 | [»] | |
BMRBi | Q9HCM9 | |||||
SMRi | Q9HCM9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 121170, 101 interactors |
IntActi | Q9HCM9, 78 interactors |
MINTi | Q9HCM9 |
STRINGi | 9606.ENSP00000365844 |
PTM databases
iPTMneti | Q9HCM9 |
PhosphoSitePlusi | Q9HCM9 |
Genetic variation databases
BioMutai | TRIM39 |
DMDMi | 56405385 |
Proteomic databases
jPOSTi | Q9HCM9 |
MassIVEi | Q9HCM9 |
MaxQBi | Q9HCM9 |
PaxDbi | Q9HCM9 |
PeptideAtlasi | Q9HCM9 |
PRIDEi | Q9HCM9 |
ProteomicsDBi | 81768 [Q9HCM9-1] 81769 [Q9HCM9-2] |
Protocols and materials databases
Antibodypediai | 34966, 268 antibodies |
DNASUi | 56658 |
Genome annotation databases
Organism-specific databases
CTDi | 56658 |
DisGeNETi | 56658 |
GeneCardsi | TRIM39 |
HGNCi | HGNC:10065, TRIM39 |
HPAi | ENSG00000204599, Low tissue specificity |
MIMi | 605700, gene |
neXtProti | NX_Q9HCM9 |
OpenTargetsi | ENSG00000204599 |
PharmGKBi | PA35535 |
VEuPathDBi | HostDB:ENSG00000204599.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00940000154126 |
HOGENOMi | CLU_013137_0_3_1 |
InParanoidi | Q9HCM9 |
OMAi | WAVGVCQ |
PhylomeDBi | Q9HCM9 |
TreeFami | TF342569 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q9HCM9 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 56658, 1 hit in 874 CRISPR screens |
ChiTaRSi | TRIM39, human |
EvolutionaryTracei | Q9HCM9 |
GenomeRNAii | 56658 |
Pharosi | Q9HCM9, Tbio |
PROi | PR:Q9HCM9 |
RNActi | Q9HCM9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000204599, Expressed in testis and 205 other tissues |
ExpressionAtlasi | Q9HCM9, baseline and differential |
Genevisiblei | Q9HCM9, HS |
Family and domain databases
CDDi | cd00021, BBOX, 1 hit cd13745, SPRY_PRY_TRIM39, 1 hit |
Gene3Di | 2.60.120.920, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR003879, Butyrophylin_SPRY IPR013320, ConA-like_dom_sf IPR006574, PRY IPR035033, PRY/SPRY_TRIM39 IPR003877, SPRY_dom IPR000315, Znf_B-box IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF13765, PRY, 1 hit PF00622, SPRY, 1 hit PF00643, zf-B_box, 1 hit |
PRINTSi | PR01407, BUTYPHLNCDUF |
SMARTi | View protein in SMART SM00336, BBOX, 1 hit SM00589, PRY, 1 hit SM00184, RING, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRI39_HUMAN | |
Accessioni | Q9HCM9Primary (citable) accession number: Q9HCM9 Secondary accession number(s): Q5STG3 Q96IB6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2001 |
Last sequence update: | December 7, 2004 | |
Last modified: | February 10, 2021 | |
This is version 191 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families