UniProtKB - Q9HCI7 (MSL2_HUMAN)
Protein
E3 ubiquitin-protein ligase MSL2
Gene
MSL2
Organism
Homo sapiens (Human)
Status
Functioni
Component of histone acetyltransferase complex responsible for the majority of histone H4 acetylation at lysine 16 which is implicated in the formation of higher-order chromatin structure. Acts as an E3 ubiquitin ligase that promotes monoubiquitination of histone H2B at 'Lys-35' (H2BK34Ub), but not that of H2A. This activity is greatly enhanced by heterodimerization with MSL1. H2B ubiquitination in turn stimulates histone H3 methylation at 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) and leads to gene activation, including that of HOXA9 and MEIS1.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 44 – 85 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ubiquitin protein ligase activity Source: GO_Central
GO - Biological processi
- histone H4-K16 acetylation Source: UniProtKB
- protein ubiquitination Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9HCI7 |
Reactomei | R-HSA-3214847, HATs acetylate histones |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase MSL2 (EC:2.3.2.-)Alternative name(s): E3 ubiquitin-protein transferase MSL2Curated Male-specific lethal 2-like 1 Short name: MSL2-like 1 Male-specific lethal-2 homolog Short name: MSL-2 Male-specific lethal-2 homolog 1 RING finger protein 184 |
Gene namesi | Name:MSL2 Synonyms:KIAA1585, MSL2L1, RNF184 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:25544, MSL2 |
MIMi | 614802, gene |
neXtProti | NX_Q9HCI7 |
VEuPathDBi | HostDB:ENSG00000174579.3 |
Subcellular locationi
Nucleus
- MSL complex Source: UniProtKB
- nucleoplasm Source: Reactome
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 64 | H → Y: Great reduction in H2B ubiquitination. No effect on MSL1-binding. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 55167 |
OpenTargetsi | ENSG00000174579 |
PharmGKBi | PA164723152 |
Miscellaneous databases
Pharosi | Q9HCI7, Tbio |
Genetic variation databases
BioMutai | MSL2 |
DMDMi | 158564022 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000299536 | 1 – 577 | E3 ubiquitin-protein ligase MSL2Add BLAST | 577 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 375 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 447 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9HCI7 |
jPOSTi | Q9HCI7 |
MassIVEi | Q9HCI7 |
MaxQBi | Q9HCI7 |
PaxDbi | Q9HCI7 |
PeptideAtlasi | Q9HCI7 |
PRIDEi | Q9HCI7 |
ProteomicsDBi | 33946 81733 [Q9HCI7-1] |
PTM databases
iPTMneti | Q9HCI7 |
PhosphoSitePlusi | Q9HCI7 |
Expressioni
Gene expression databases
Bgeei | ENSG00000174579, Expressed in leukocyte and 236 other tissues |
ExpressionAtlasi | Q9HCI7, baseline and differential |
Genevisiblei | Q9HCI7, HS |
Organism-specific databases
HPAi | ENSG00000174579, Low tissue specificity |
Interactioni
Subunit structurei
Protein-protein interaction databases
BioGRIDi | 120467, 30 interactors |
ComplexPortali | CPX-815, MSL histone acetyltransferase complex |
CORUMi | Q9HCI7 |
IntActi | Q9HCI7, 16 interactors |
STRINGi | 9606.ENSP00000311827 |
Miscellaneous databases
RNActi | Q9HCI7, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9HCI7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 116 | Sufficient for interaction with MSL11 PublicationAdd BLAST | 116 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 396 – 463 | Lys-richAdd BLAST | 68 |
Sequence similaritiesi
Belongs to the MSL2 family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 44 – 85 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | ENOG502QPJR, Eukaryota |
GeneTreei | ENSGT00390000016814 |
HOGENOMi | CLU_038772_0_0_1 |
InParanoidi | Q9HCI7 |
OMAi | CEGCSDF |
OrthoDBi | 1446941at2759 |
PhylomeDBi | Q9HCI7 |
TreeFami | TF328848 |
Family and domain databases
CDDi | cd13122, MSL2_CXC, 1 hit |
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR037922, MSL2 IPR032049, Msl2-CXC IPR032043, Msl2_Znf-RING IPR033467, Tesmin/TSO1-like_CXC IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR16048, PTHR16048, 1 hit |
Pfami | View protein in Pfam PF16682, MSL2-CXC, 1 hit PF16685, zf-RING_10, 1 hit |
SMARTi | View protein in SMART SM01114, CXC, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9HCI7-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNPVNATALY ISASRLVLNY DPGDPKAFTE INRLLPYFRQ SLSCCVCGHL
60 70 80 90 100
LQDPIAPTNS TCQHYVCKTC KGKKMMMKPS CSWCKDYEQF EENKQLSILV
110 120 130 140 150
NCYKKLCEYI TQTTLARDII EAVDCSSDIL ALLNDGSLFC EETEKPSDSS
160 170 180 190 200
FTLCLTHSPL PSTSEPTTDP QASLSPMSES TLSIAIGSSV INGLPTYNGL
210 220 230 240 250
SIDRFGINIP SPEHSNTIDV CNTVDIKTED LSDSLPPVCD TVATDLCSTG
260 270 280 290 300
IDICSFSEDI KPGDSLLLSV EEVLRSLETV SNTEVCCPNL QPNLEATVSN
310 320 330 340 350
GPFLQLSSQS LSHNVFMSTS PALHGLSCTA ATPKIAKLNR KRSRSESDSE
360 370 380 390 400
KVQPLPISTI IRGPTLGASA PVTVKRESKI SLQPIATVPN GGTTPKISKT
410 420 430 440 450
VLLSTKSMKK SHEHGSKKSH SKTKPGILKK DKAVKEKIPS HHFMPGSPTK
460 470 480 490 500
TVYKKPQEKK GCKCGRATQN PSVLTCRGQR CPCYSNRKAC LDCICRGCQN
510 520 530 540 550
SYMANGEKKL EAFAVPEKAL EQTRLTLGIN VTSIAVRNAS TSTSVINVTG
560 570
SPVTTFLAAS THDDKSLDEA IDMRFDC
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9IZ03 | C9IZ03_HUMAN | E3 ubiquitin-protein ligase MSL2 | MSL2 | 154 | Annotation score: | ||
C9IZG7 | C9IZG7_HUMAN | E3 ubiquitin-protein ligase MSL2 | MSL2 | 95 | Annotation score: | ||
C9J5S2 | C9J5S2_HUMAN | E3 ubiquitin-protein ligase MSL2 | MSL2 | 149 | Annotation score: |
Sequence cautioni
The sequence AAH32719 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence BAA91673 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAB13411 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 348 | D → E in BAG63776 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 411 | S → G in BAA91673 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046200 | 1 – 74 | Missing in isoform 2. 1 PublicationAdd BLAST | 74 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB046805 mRNA Translation: BAB13411.1 Different initiation. AK001408 mRNA Translation: BAA91673.1 Different initiation. AK302491 mRNA Translation: BAG63776.1 AL834289 mRNA Translation: CAD38963.2 AC092991 Genomic DNA No translation available. CH471052 Genomic DNA Translation: EAW79119.1 CH471052 Genomic DNA Translation: EAW79120.1 BC032719 mRNA Translation: AAH32719.1 Different initiation. BC093764 mRNA Translation: AAH93764.1 BC093790 mRNA Translation: AAH93790.1 |
CCDSi | CCDS33861.1 [Q9HCI7-1] CCDS46922.1 [Q9HCI7-2] |
RefSeqi | NP_001138889.1, NM_001145417.1 [Q9HCI7-2] NP_060603.2, NM_018133.3 [Q9HCI7-1] XP_005247628.1, XM_005247571.3 [Q9HCI7-2] XP_006713747.1, XM_006713684.3 [Q9HCI7-2] XP_011511251.1, XM_011512949.2 [Q9HCI7-2] |
Genome annotation databases
Ensembli | ENST00000309993; ENSP00000311827; ENSG00000174579 [Q9HCI7-1] ENST00000434835; ENSP00000387948; ENSG00000174579 [Q9HCI7-2] |
GeneIDi | 55167 |
KEGGi | hsa:55167 |
UCSCi | uc003eqx.2, human [Q9HCI7-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB046805 mRNA Translation: BAB13411.1 Different initiation. AK001408 mRNA Translation: BAA91673.1 Different initiation. AK302491 mRNA Translation: BAG63776.1 AL834289 mRNA Translation: CAD38963.2 AC092991 Genomic DNA No translation available. CH471052 Genomic DNA Translation: EAW79119.1 CH471052 Genomic DNA Translation: EAW79120.1 BC032719 mRNA Translation: AAH32719.1 Different initiation. BC093764 mRNA Translation: AAH93764.1 BC093790 mRNA Translation: AAH93790.1 |
CCDSi | CCDS33861.1 [Q9HCI7-1] CCDS46922.1 [Q9HCI7-2] |
RefSeqi | NP_001138889.1, NM_001145417.1 [Q9HCI7-2] NP_060603.2, NM_018133.3 [Q9HCI7-1] XP_005247628.1, XM_005247571.3 [Q9HCI7-2] XP_006713747.1, XM_006713684.3 [Q9HCI7-2] XP_011511251.1, XM_011512949.2 [Q9HCI7-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4B7Y | X-ray | 3.25 | C/D | 1-116 | [»] | |
4B86 | X-ray | 3.50 | C/D/G/H/K/L | 1-116 | [»] | |
SMRi | Q9HCI7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 120467, 30 interactors |
ComplexPortali | CPX-815, MSL histone acetyltransferase complex |
CORUMi | Q9HCI7 |
IntActi | Q9HCI7, 16 interactors |
STRINGi | 9606.ENSP00000311827 |
PTM databases
iPTMneti | Q9HCI7 |
PhosphoSitePlusi | Q9HCI7 |
Genetic variation databases
BioMutai | MSL2 |
DMDMi | 158564022 |
Proteomic databases
EPDi | Q9HCI7 |
jPOSTi | Q9HCI7 |
MassIVEi | Q9HCI7 |
MaxQBi | Q9HCI7 |
PaxDbi | Q9HCI7 |
PeptideAtlasi | Q9HCI7 |
PRIDEi | Q9HCI7 |
ProteomicsDBi | 33946 81733 [Q9HCI7-1] |
Protocols and materials databases
Antibodypediai | 1091, 161 antibodies |
Genome annotation databases
Ensembli | ENST00000309993; ENSP00000311827; ENSG00000174579 [Q9HCI7-1] ENST00000434835; ENSP00000387948; ENSG00000174579 [Q9HCI7-2] |
GeneIDi | 55167 |
KEGGi | hsa:55167 |
UCSCi | uc003eqx.2, human [Q9HCI7-1] |
Organism-specific databases
CTDi | 55167 |
DisGeNETi | 55167 |
GeneCardsi | MSL2 |
HGNCi | HGNC:25544, MSL2 |
HPAi | ENSG00000174579, Low tissue specificity |
MIMi | 614802, gene |
neXtProti | NX_Q9HCI7 |
OpenTargetsi | ENSG00000174579 |
PharmGKBi | PA164723152 |
VEuPathDBi | HostDB:ENSG00000174579.3 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QPJR, Eukaryota |
GeneTreei | ENSGT00390000016814 |
HOGENOMi | CLU_038772_0_0_1 |
InParanoidi | Q9HCI7 |
OMAi | CEGCSDF |
OrthoDBi | 1446941at2759 |
PhylomeDBi | Q9HCI7 |
TreeFami | TF328848 |
Enzyme and pathway databases
PathwayCommonsi | Q9HCI7 |
Reactomei | R-HSA-3214847, HATs acetylate histones |
Miscellaneous databases
BioGRID-ORCSi | 55167, 20 hits in 880 CRISPR screens |
ChiTaRSi | MSL2, human |
GenomeRNAii | 55167 |
Pharosi | Q9HCI7, Tbio |
PROi | PR:Q9HCI7 |
RNActi | Q9HCI7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000174579, Expressed in leukocyte and 236 other tissues |
ExpressionAtlasi | Q9HCI7, baseline and differential |
Genevisiblei | Q9HCI7, HS |
Family and domain databases
CDDi | cd13122, MSL2_CXC, 1 hit |
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR037922, MSL2 IPR032049, Msl2-CXC IPR032043, Msl2_Znf-RING IPR033467, Tesmin/TSO1-like_CXC IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR16048, PTHR16048, 1 hit |
Pfami | View protein in Pfam PF16682, MSL2-CXC, 1 hit PF16685, zf-RING_10, 1 hit |
SMARTi | View protein in SMART SM01114, CXC, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MSL2_HUMAN | |
Accessioni | Q9HCI7Primary (citable) accession number: Q9HCI7 Secondary accession number(s): B4DYL4 Q9NVS4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 11, 2007 |
Last sequence update: | September 11, 2007 | |
Last modified: | February 10, 2021 | |
This is version 136 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM