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UniProtKB - Q9HCI7 (MSL2_HUMAN)

Protein

E3 ubiquitin-protein ligase MSL2

Gene

MSL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of histone acetyltransferase complex responsible for the majority of histone H4 acetylation at lysine 16 which is implicated in the formation of higher-order chromatin structure. Acts as an E3 ubiquitin ligase that promotes monoubiquitination of histone H2B at 'Lys-35' (H2BK34Ub), but not that of H2A. This activity is greatly enhanced by heterodimerization with MSL1. H2B ubiquitination in turn stimulates histine H3 methylation at 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) and leads to gene activation, including that of HOXA9 and MEIS1.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri44 – 85RING-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • ubiquitin protein ligase activity Source: GO_Central
View the complete GO annotation on QuickGO ...

GO - Biological processi

  • histone H4-K16 acetylation Source: UniProtKB
  • protein ubiquitination Source: GO_Central
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-3214847 HATs acetylate histones

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase MSL2 (EC:2.3.2.-)
Alternative name(s):
E3 ubiquitin-protein transferase MSL2Curated
Male-specific lethal 2-like 1
Short name:
MSL2-like 1
Male-specific lethal-2 homolog
Short name:
MSL-2
Male-specific lethal-2 homolog 1
RING finger protein 184
Gene namesi
Name:MSL2
Synonyms:KIAA1585, MSL2L1, RNF184
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000174579.3
HGNCiHGNC:25544 MSL2
MIMi614802 gene
neXtProtiNX_Q9HCI7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi64H → Y: Great redution in H2B ubiquitination. No effect on MSL1-binding. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000174579
PharmGKBiPA164723152

Polymorphism and mutation databases

BioMutaiMSL2
DMDMi158564022

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002995361 – 577E3 ubiquitin-protein ligase MSL2Add BLAST577

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki375Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei447PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9HCI7
MaxQBiQ9HCI7
PaxDbiQ9HCI7
PeptideAtlasiQ9HCI7
PRIDEiQ9HCI7
ProteomicsDBi81733

PTM databases

iPTMnetiQ9HCI7
PhosphoSitePlusiQ9HCI7

Expressioni

Gene expression databases

BgeeiENSG00000174579 Expressed in 225 organ(s), highest expression level in leukocyte
CleanExiHS_MSL2
ExpressionAtlasiQ9HCI7 baseline and differential
GenevisibleiQ9HCI7 HS

Organism-specific databases

HPAiHPA003413
HPA048482

Interactioni

Subunit structurei

Component of a multisubunit histone acetyltransferase complex (MSL) at least composed of the KAT8/MOF/MYST1, MSL1/hampin, MSL2 and MSL3. Forms a MSL heterotetrameric core with MSL1.1 Publication

Protein-protein interaction databases

BioGridi120467, 26 interactors
ComplexPortaliCPX-815 MSL histone acetyltransferase complex
CORUMiQ9HCI7
IntActiQ9HCI7, 8 interactors
STRINGi9606.ENSP00000311827

Structurei

Secondary structure

1577
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9HCI7
SMRiQ9HCI7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 116Sufficient for interaction with MSL11 PublicationAdd BLAST116

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi396 – 463Lys-richAdd BLAST68

Sequence similaritiesi

Belongs to the MSL2 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri44 – 85RING-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IGUA Eukaryota
ENOG410ZJAZ LUCA
GeneTreeiENSGT00390000016814
HOGENOMiHOG000043091
HOVERGENiHBG108149
InParanoidiQ9HCI7
KOiK13164
OMAiNVMPGSP
OrthoDBiEOG091G0IAA
PhylomeDBiQ9HCI7
TreeFamiTF328848

Family and domain databases

CDDicd13122 MSL2_CXC, 1 hit
Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR037922 MSL2
IPR032049 Msl2-CXC
IPR032043 Msl2_Znf-RING
IPR033467 Tesmin/TSO1-like_CXC
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR16048 PTHR16048, 1 hit
PfamiView protein in Pfam
PF16682 MSL2-CXC, 1 hit
PF16685 zf-RING_10, 1 hit
SMARTiView protein in SMART
SM01114 CXC, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9HCI7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNPVNATALY ISASRLVLNY DPGDPKAFTE INRLLPYFRQ SLSCCVCGHL 
        60         70         80         90        100
LQDPIAPTNS TCQHYVCKTC KGKKMMMKPS CSWCKDYEQF EENKQLSILV 
       110        120        130        140        150
NCYKKLCEYI TQTTLARDII EAVDCSSDIL ALLNDGSLFC EETEKPSDSS 
       160        170        180        190        200
FTLCLTHSPL PSTSEPTTDP QASLSPMSES TLSIAIGSSV INGLPTYNGL 
       210        220        230        240        250
SIDRFGINIP SPEHSNTIDV CNTVDIKTED LSDSLPPVCD TVATDLCSTG 
       260        270        280        290        300
IDICSFSEDI KPGDSLLLSV EEVLRSLETV SNTEVCCPNL QPNLEATVSN 
       310        320        330        340        350
GPFLQLSSQS LSHNVFMSTS PALHGLSCTA ATPKIAKLNR KRSRSESDSE 
       360        370        380        390        400
KVQPLPISTI IRGPTLGASA PVTVKRESKI SLQPIATVPN GGTTPKISKT 
       410        420        430        440        450
VLLSTKSMKK SHEHGSKKSH SKTKPGILKK DKAVKEKIPS HHFMPGSPTK 
       460        470        480        490        500
TVYKKPQEKK GCKCGRATQN PSVLTCRGQR CPCYSNRKAC LDCICRGCQN 
       510        520        530        540        550
SYMANGEKKL EAFAVPEKAL EQTRLTLGIN VTSIAVRNAS TSTSVINVTG 
       560        570 
SPVTTFLAAS THDDKSLDEA IDMRFDC
Length:577
Mass (Da):62,541
Last modified:September 11, 2007 - v2
Checksum:i756C7625E0AF1CA6
GO
Isoform 2 (identifier: Q9HCI7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-74: Missing.

Note: No experimental confirmation available.
Show »
Length:503
Mass (Da):54,339
Checksum:iF591F7182AA9937D
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9IZ03C9IZ03_HUMAN
E3 ubiquitin-protein ligase MSL2
MSL2
154Annotation score:
C9J5S2C9J5S2_HUMAN
E3 ubiquitin-protein ligase MSL2
MSL2
149Annotation score:
C9IZG7C9IZG7_HUMAN
E3 ubiquitin-protein ligase MSL2
MSL2
95Annotation score:

Sequence cautioni

The sequence AAH32719 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAA91673 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB13411 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti348D → E in BAG63776 (PubMed:14702039).Curated1
Sequence conflicti411S → G in BAA91673 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0462001 – 74Missing in isoform 2. 1 PublicationAdd BLAST74

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046805 mRNA Translation: BAB13411.1 Different initiation.
AK001408 mRNA Translation: BAA91673.1 Different initiation.
AK302491 mRNA Translation: BAG63776.1
AL834289 mRNA Translation: CAD38963.2
AC092991 Genomic DNA No translation available.
CH471052 Genomic DNA Translation: EAW79119.1
CH471052 Genomic DNA Translation: EAW79120.1
BC032719 mRNA Translation: AAH32719.1 Different initiation.
BC093764 mRNA Translation: AAH93764.1
BC093790 mRNA Translation: AAH93790.1
CCDSiCCDS33861.1 [Q9HCI7-1]
CCDS46922.1 [Q9HCI7-2]
RefSeqiNP_001138889.1, NM_001145417.1 [Q9HCI7-2]
NP_060603.2, NM_018133.3 [Q9HCI7-1]
XP_005247628.1, XM_005247571.3 [Q9HCI7-2]
XP_006713747.1, XM_006713684.3 [Q9HCI7-2]
XP_011511251.1, XM_011512949.2 [Q9HCI7-2]
UniGeneiHs.18631

Genome annotation databases

EnsembliENST00000309993; ENSP00000311827; ENSG00000174579 [Q9HCI7-1]
ENST00000434835; ENSP00000387948; ENSG00000174579 [Q9HCI7-2]
GeneIDi55167
KEGGihsa:55167
UCSCiuc003eqx.2 human [Q9HCI7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046805 mRNA Translation: BAB13411.1 Different initiation.
AK001408 mRNA Translation: BAA91673.1 Different initiation.
AK302491 mRNA Translation: BAG63776.1
AL834289 mRNA Translation: CAD38963.2
AC092991 Genomic DNA No translation available.
CH471052 Genomic DNA Translation: EAW79119.1
CH471052 Genomic DNA Translation: EAW79120.1
BC032719 mRNA Translation: AAH32719.1 Different initiation.
BC093764 mRNA Translation: AAH93764.1
BC093790 mRNA Translation: AAH93790.1
CCDSiCCDS33861.1 [Q9HCI7-1]
CCDS46922.1 [Q9HCI7-2]
RefSeqiNP_001138889.1, NM_001145417.1 [Q9HCI7-2]
NP_060603.2, NM_018133.3 [Q9HCI7-1]
XP_005247628.1, XM_005247571.3 [Q9HCI7-2]
XP_006713747.1, XM_006713684.3 [Q9HCI7-2]
XP_011511251.1, XM_011512949.2 [Q9HCI7-2]
UniGeneiHs.18631

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4B7YX-ray3.25C/D1-116[»]
4B86X-ray3.50C/D/G/H/K/L1-116[»]
ProteinModelPortaliQ9HCI7
SMRiQ9HCI7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120467, 26 interactors
ComplexPortaliCPX-815 MSL histone acetyltransferase complex
CORUMiQ9HCI7
IntActiQ9HCI7, 8 interactors
STRINGi9606.ENSP00000311827

PTM databases

iPTMnetiQ9HCI7
PhosphoSitePlusiQ9HCI7

Polymorphism and mutation databases

BioMutaiMSL2
DMDMi158564022

Proteomic databases

EPDiQ9HCI7
MaxQBiQ9HCI7
PaxDbiQ9HCI7
PeptideAtlasiQ9HCI7
PRIDEiQ9HCI7
ProteomicsDBi81733

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000309993; ENSP00000311827; ENSG00000174579 [Q9HCI7-1]
ENST00000434835; ENSP00000387948; ENSG00000174579 [Q9HCI7-2]
GeneIDi55167
KEGGihsa:55167
UCSCiuc003eqx.2 human [Q9HCI7-1]

Organism-specific databases

CTDi55167
EuPathDBiHostDB:ENSG00000174579.3
GeneCardsiMSL2
HGNCiHGNC:25544 MSL2
HPAiHPA003413
HPA048482
MIMi614802 gene
neXtProtiNX_Q9HCI7
OpenTargetsiENSG00000174579
PharmGKBiPA164723152
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IGUA Eukaryota
ENOG410ZJAZ LUCA
GeneTreeiENSGT00390000016814
HOGENOMiHOG000043091
HOVERGENiHBG108149
InParanoidiQ9HCI7
KOiK13164
OMAiNVMPGSP
OrthoDBiEOG091G0IAA
PhylomeDBiQ9HCI7
TreeFamiTF328848

Enzyme and pathway databases

ReactomeiR-HSA-3214847 HATs acetylate histones

Miscellaneous databases

ChiTaRSiMSL2 human
GenomeRNAii55167
PROiPR:Q9HCI7
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000174579 Expressed in 225 organ(s), highest expression level in leukocyte
CleanExiHS_MSL2
ExpressionAtlasiQ9HCI7 baseline and differential
GenevisibleiQ9HCI7 HS

Family and domain databases

CDDicd13122 MSL2_CXC, 1 hit
Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR037922 MSL2
IPR032049 Msl2-CXC
IPR032043 Msl2_Znf-RING
IPR033467 Tesmin/TSO1-like_CXC
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR16048 PTHR16048, 1 hit
PfamiView protein in Pfam
PF16682 MSL2-CXC, 1 hit
PF16685 zf-RING_10, 1 hit
SMARTiView protein in SMART
SM01114 CXC, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMSL2_HUMAN
AccessioniPrimary (citable) accession number: Q9HCI7
Secondary accession number(s): B4DYL4
, G5E9I1, Q0D2P1, Q8NDB4, Q9NVS4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: November 7, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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