SMURF1

Functionⁱ

E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed:23087404). Plays a role in dendrite formation by melanocytes (PubMed:23999003).5 Publications

Catalytic activityⁱ

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayⁱ: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	725	Glycyl thioester intermediate		1

GO - Molecular functionⁱ

activin binding
Source: BHF-UCL
Non-traceable author statementⁱ
- 16720724
I-SMAD binding
Source: BHF-UCL
Inferred from physical interactionⁱ
- 11278251
- 12151385
phospholipid binding
Source: ParkinsonsUK-UCL
Traceable author statementⁱ
- 22020285
R-SMAD binding
Source: BHF-UCL
Inferred from physical interactionⁱ
- 11278251
ubiquitin protein ligase activity
Source: BHF-UCL
Inferred from direct assayⁱ
- 11278251
- 12151385
ubiquitin-protein transferase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 10458166
- 19937093

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

BMP signaling pathway
Source: UniProtKB
Inferred from direct assayⁱ
- 21572392
cell differentiation
Source: UniProtKB
Inferred from direct assayⁱ
- 10458166
ectoderm development
Source: UniProtKB
Traceable author statementⁱ
- 10458166
engulfment of target by autophagosome
Source: ParkinsonsUK-UCL
Inferred from mutant phenotypeⁱ
- 22020285
negative regulation of BMP signaling pathway
Source: BHF-UCL
Inferred from direct assayⁱ
- 27214554
negative regulation of ossification Source: Ensembl
negative regulation of transforming growth factor beta receptor signaling pathway
Source: BHF-UCL
Inferred from direct assayⁱ
- 11278251
- 12151385
parkin-mediated stimulation of mitophagy in response to mitochondrial depolarization Source: Ensembl
positive regulation of dendrite extension
Source: UniProtKB
Inferred from direct assayⁱ
- 23999003
positive regulation of protein catabolic process
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
positive regulation of ubiquitin-dependent protein catabolic process
Source: CACAO
Inferred from mutant phenotypeⁱ
- 21454619
proteasome-mediated ubiquitin-dependent protein catabolic process
Source: BHF-UCL
Inferred from direct assayⁱ
- 11278251
protein export from nucleus
Source: BHF-UCL
Inferred from direct assayⁱ
- 11278251
protein localization to cell surface
Source: BHF-UCL
Inferred from direct assayⁱ
- 12151385
protein localization to plasma membrane
Source: ParkinsonsUK-UCL
Traceable author statementⁱ
- 22020285
protein polyubiquitination
Source: UniProtKB
Inferred from direct assayⁱ
- 19937093
protein targeting to vacuole involved in autophagy
Source: ParkinsonsUK-UCL
Inferred from mutant phenotypeⁱ
- 22020285
protein ubiquitination
Source: BHF-UCL
Inferred from direct assayⁱ
- 11278251
receptor catabolic process
Source: BHF-UCL
Inferred from direct assayⁱ
- 12151385
substrate localization to autophagosome
Source: ParkinsonsUK-UCL
Inferred from mutant phenotypeⁱ
- 22020285
transforming growth factor beta receptor signaling pathway Source: Reactome
ubiquitin-dependent protein catabolic process
Source: UniProtKB
Inferred from direct assayⁱ
- 10458166
- 19937093
ubiquitin-dependent SMAD protein catabolic process
Source: BHF-UCL
Inferred from direct assayⁱ
- 11278251
- 12151385
Wnt signaling pathway, planar cell polarity pathway Source: Reactome

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Differentiation, Ubl conjugation pathway

Enzyme and pathway databases

BRENDAⁱ	2.3.2.B9 2681 6.3.2.19 2681
Reactomeⁱ	R-HSA-201451 Signaling by BMP R-HSA-2173788 Downregulation of TGF-beta receptor signaling R-HSA-2173791 TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) R-HSA-4608870 Asymmetric localization of PCP proteins R-HSA-5632684 Hedgehog 'on' state R-HSA-8939902 Regulation of RUNX2 expression and activity R-HSA-8941858 Regulation of RUNX3 expression and activity R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkⁱ	Q9HCE7
SIGNORⁱ	Q9HCE7
UniPathwayⁱ	UPA00143

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: E3 ubiquitin-protein ligase SMURF1 (EC:2.3.2.261 Publication) Short name: hSMURF1 Alternative name(s): HECT-type E3 ubiquitin transferase SMURF1 SMAD ubiquitination regulatory factor 1 SMAD-specific E3 ubiquitin-protein ligase 1
Gene namesⁱ	Name:SMURF1 Synonyms:KIAA1625
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 7

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000198742.9
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:16807 SMURF1
MIMⁱ	605568 gene
neXtProtⁱ	NX_Q9HCE7

Keywords - Cellular componentⁱ

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	28	K → A: Fails to ubiquitinate RHOA; when associated with A-85. 1 Publication	1
Mutagenesisⁱ	85	K → A: Fails to ubiquitinate RHOA; when associated with A-28. 1 Publication	1
Mutagenesisⁱ	350	K → R: Abolishes FBXL15-mediated ubiquitination and degradation; when associated with R-381 and R-381. 1 Publication	1
Mutagenesisⁱ	381	K → R: Abolishes FBXL15-mediated ubiquitination and degradation; when associated with R-350 and R-383. Abolishes FBXL15-mediated ubiquitination and degradation; when associated with R-383. 1 Publication	1
Mutagenesisⁱ	383	K → R: Abolishes FBXL15-mediated ubiquitination and degradation; when associated with R-350 and R-381. Abolishes FBXL15-mediated ubiquitination and degradation; when associated with R-381. 1 Publication	1
Mutagenesisⁱ	725	C → A: Loss of enzyme activity, without abolishing FBXL15-mediated ubiquitination. 2 Publications	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	57154
Open Targets More...OpenTargetsⁱ	ENSG00000198742
PharmGKBⁱ	PA134987175

Chemistry databases

ChEMBLⁱ	CHEMBL3879859

ChEMBLⁱ

CHEMBL3879859

Polymorphism and mutation databases

BioMutaⁱ	SMURF1
DMDMⁱ	17865625

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000120326	1 – 757	E3 ubiquitin-protein ligase SMURF1Add BLAST		757

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	200	PhosphoserineCombined sources	1
Cross-linkⁱ	381	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linkⁱ	383	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationⁱ

Auto-ubiquitinated in presence of NDFIP1 (PubMed:23087404). Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.2 Publications

Keywords - PTMⁱ

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	Q9HCE7
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9HCE7
PaxDbⁱ	Q9HCE7
PeptideAtlas More...PeptideAtlasⁱ	Q9HCE7
PRIDEⁱ	Q9HCE7
ProteomicsDBⁱ	81693 81694 [Q9HCE7-2]

PTM databases

iPTMnetⁱ	Q9HCE7
PhosphoSitePlusⁱ	Q9HCE7

Expressionⁱ

Tissue specificityⁱ

Expressed in melanocytes (PubMed:23999003).1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000198742 Expressed in 201 organ(s), highest expression level in testis
CleanExⁱ	HS_SMURF1
Genevisibleⁱ	Q9HCE7 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA055245

HPAⁱ

HPA055245

Interactionⁱ

Subunit structureⁱ

Interacts with TRAF4. Interacts (via HECT domain) with FBXL15 (via LRR repeats). Interacts with SMAD7 and TGFBR1; SMAD7 recruits SMURF1 to TGFBR1 and regulates TGF-beta receptor degradation. Interacts with MAVS; the interaction is mediated by NDFIP1 (PubMed:23087404).5 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
CHEK1	O14757	4	EBI-9845742,EBI-974488
FBXL15	Q9H469	6	EBI-976466,EBI-6144096
ING2	Q9H160	3	EBI-9845742,EBI-389787
KLF2	Q9Y5W3	4	EBI-9845742,EBI-9846663
PLEKHO1	Q53GL0	4	EBI-9845742,EBI-949945
PSME3	P61289	5	EBI-976466,EBI-355546
RHOA	P61586	2	EBI-9845742,EBI-446668
RHOB	P62745	3	EBI-9845742,EBI-602647
SMAD1	Q15797	2	EBI-976466,EBI-1567153
SMAD7	O15105	4	EBI-15884081,EBI-3861591
TRAF4	Q9BUZ4	4	EBI-9845742,EBI-3650647

GO - Molecular functionⁱ

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	121411, 416 interactors
CORUMⁱ	Q9HCE7
Database of interacting proteins More...DIPⁱ	DIP-36709N
IntActⁱ	Q9HCE7, 25 interactors
Molecular INTeraction database More...MINTⁱ	Q9HCE7
STRINGⁱ	9606.ENSP00000354621

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	13 – 24	Combined sources	12
Beta strandⁱ	36 – 42	Combined sources	7
Turnⁱ	43 – 45	Combined sources	3
Beta strandⁱ	48 – 50	Combined sources	3
Beta strandⁱ	61 – 71	Combined sources	11
Beta strandⁱ	76 – 82	Combined sources	7
Helixⁱ	83 – 85	Combined sources	3
Turnⁱ	90 – 93	Combined sources	4
Beta strandⁱ	94 – 100	Combined sources	7
Helixⁱ	102 – 108	Combined sources	7
Beta strandⁱ	114 – 117	Combined sources	4
Beta strandⁱ	131 – 139	Combined sources	9
Beta strandⁱ	241 – 245	Combined sources	5
Turnⁱ	246 – 248	Combined sources	3
Beta strandⁱ	249 – 254	Combined sources	6
Turnⁱ	255 – 258	Combined sources	4
Beta strandⁱ	259 – 263	Combined sources	5
Beta strandⁱ	312 – 317	Combined sources	6
Turnⁱ	318 – 320	Combined sources	3
Beta strandⁱ	321 – 326	Combined sources	6
Turnⁱ	327 – 330	Combined sources	4
Beta strandⁱ	331 – 334	Combined sources	4

Show more details

3D structure databases

ProteinModelPortalⁱ	Q9HCE7
SMRⁱ	Q9HCE7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q9HCE7

EvolutionaryTraceⁱ

Q9HCE7

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	1 – 99	C2PROSITE-ProRule annotationAdd BLAST	99
Domainⁱ	234 – 267	WW 1PROSITE-ProRule annotationAdd BLAST	34
Domainⁱ	306 – 339	WW 2PROSITE-ProRule annotationAdd BLAST	34
Domainⁱ	420 – 757	HECTPROSITE-ProRule annotationAdd BLAST	338

Domainⁱ

The C2 domain mediates membrane localization and substrate selection.1 Publication

Keywords - Domainⁱ

Repeat

Phylogenomic databases

eggNOGⁱ	KOG0940 Eukaryota COG5021 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118966
HOGENOMⁱ	HOG000208451
HOVERGENⁱ	HBG004134
InParanoidⁱ	Q9HCE7
KEGG Orthology (KO) More...KOⁱ	K04678
OMAⁱ	CKLNPTD
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G011S
PhylomeDBⁱ	Q9HCE7
TreeFamⁱ	TF323658

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00078 HECTc, 1 hit cd00201 WW, 2 hits
Gene3Dⁱ	2.60.40.150, 1 hit
InterProⁱ	View protein in InterPro IPR000008 C2_dom IPR035892 C2_domain_sf IPR024928 E3_ub_ligase_SMURF1 IPR000569 HECT_dom IPR035983 Hect_E3_ubiquitin_ligase IPR001202 WW_dom IPR036020 WW_dom_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00168 C2, 1 hit PF00632 HECT, 1 hit PF00397 WW, 2 hits
PIRSFⁱ	PIRSF001569 E3_ub_ligase_SMURF1, 1 hit
SMARTⁱ	View protein in SMART SM00239 C2, 1 hit SM00119 HECTc, 1 hit SM00456 WW, 2 hits
SUPFAMⁱ	SSF51045 SSF51045, 2 hits SSF56204 SSF56204, 1 hit
PROSITEⁱ	View protein in PROSITE PS50004 C2, 1 hit PS50237 HECT, 1 hit PS01159 WW_DOMAIN_1, 1 hit PS50020 WW_DOMAIN_2, 2 hits

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

Isoform Long (identifier: Q9HCE7-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSNPGTRRNG SSIKIRLTVL CAKNLAKKDF FRLPDPFAKI VVDGSGQCHS 
        60         70         80         90        100
TDTVKNTLDP KWNQHYDLYV GKTDSITISV WNHKKIHKKQ GAGFLGCVRL 
       110        120        130        140        150
LSNAISRLKD TGYQRLDLCK LNPSDTDAVR GQIVVSLQTR DRIGTGGSVV 
       160        170        180        190        200
DCRGLLENEG TVYEDSGPGR PLSCFMEEPA PYTDSTGAAA GGGNCRFVES 
       210        220        230        240        250
PSQDQRLQAQ RLRNPDVRGS LQTPQNRPHG HQSPELPEGY EQRTTVQGQV 
       260        270        280        290        300
YFLHTQTGVS TWHDPRIPSP SGTIPGGDAA FLYEFLLQGH TSEPRDLNSV 
       310        320        330        340        350
NCDELGPLPP GWEVRSTVSG RIYFVDHNNR TTQFTDPRLH HIMNHQCQLK 
       360        370        380        390        400
EPSQPLPLPS EGSLEDEELP AQRYERDLVQ KLKVLRHELS LQQPQAGHCR 
       410        420        430        440        450
IEVSREEIFE ESYRQIMKMR PKDLKKRLMV KFRGEEGLDY GGVAREWLYL 
       460        470        480        490        500
LCHEMLNPYY GLFQYSTDNI YMLQINPDSS INPDHLSYFH FVGRIMGLAV 
       510        520        530        540        550
FHGHYINGGF TVPFYKQLLG KPIQLSDLES VDPELHKSLV WILENDITPV 
       560        570        580        590        600
LDHTFCVEHN AFGRILQHEL KPNGRNVPVT EENKKEYVRL YVNWRFMRGI 
       610        620        630        640        650
EAQFLALQKG FNELIPQHLL KPFDQKELEL IIGGLDKIDL NDWKSNTRLK 
       660        670        680        690        700
HCVADSNIVR WFWQAVETFD EERRARLLQF VTGSTRVPLQ GFKALQGSTG 
       710        720        730        740        750
AAGPRLFTIH LIDANTDNLP KAHTCFNRID IPPYESYEKL YEKLLTAVEE 

TCGFAVE

Length:757

Mass (Da):86,114

Last modified:December 13, 2001 - v2

Checksum:ⁱ89A171CFC47B40E9

GO

Isoform Short (identifier: Q9HCE7-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
269-294: Missing.

« Hide

        10         20         30         40         50
MSNPGTRRNG SSIKIRLTVL CAKNLAKKDF FRLPDPFAKI VVDGSGQCHS 
        60         70         80         90        100
TDTVKNTLDP KWNQHYDLYV GKTDSITISV WNHKKIHKKQ GAGFLGCVRL 
       110        120        130        140        150
LSNAISRLKD TGYQRLDLCK LNPSDTDAVR GQIVVSLQTR DRIGTGGSVV 
       160        170        180        190        200
DCRGLLENEG TVYEDSGPGR PLSCFMEEPA PYTDSTGAAA GGGNCRFVES 
       210        220        230        240        250
PSQDQRLQAQ RLRNPDVRGS LQTPQNRPHG HQSPELPEGY EQRTTVQGQV 
       260        270        280        290        300
YFLHTQTGVS TWHDPRIPRD LNSVNCDELG PLPPGWEVRS TVSGRIYFVD 
       310        320        330        340        350
HNNRTTQFTD PRLHHIMNHQ CQLKEPSQPL PLPSEGSLED EELPAQRYER 
       360        370        380        390        400
DLVQKLKVLR HELSLQQPQA GHCRIEVSRE EIFEESYRQI MKMRPKDLKK 
       410        420        430        440        450
RLMVKFRGEE GLDYGGVARE WLYLLCHEML NPYYGLFQYS TDNIYMLQIN 
       460        470        480        490        500
PDSSINPDHL SYFHFVGRIM GLAVFHGHYI NGGFTVPFYK QLLGKPIQLS 
       510        520        530        540        550
DLESVDPELH KSLVWILEND ITPVLDHTFC VEHNAFGRIL QHELKPNGRN 
       560        570        580        590        600
VPVTEENKKE YVRLYVNWRF MRGIEAQFLA LQKGFNELIP QHLLKPFDQK 
       610        620        630        640        650
ELELIIGGLD KIDLNDWKSN TRLKHCVADS NIVRWFWQAV ETFDEERRAR 
       660        670        680        690        700
LLQFVTGSTR VPLQGFKALQ GSTGAAGPRL FTIHLIDANT DNLPKAHTCF 
       710        720        730 
NRIDIPPYES YEKLYEKLLT AVEETCGFAV E

Show »

Length:731

Mass (Da):83,440

Checksum:ⁱF106E00CA3C210AA

GO

Sequence cautionⁱ

The sequence BAB13451 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	447	W → G in AAI44415 (PubMed:15489334).Curated	1
Sequence conflictⁱ	697 – 699	Missing in AAI36805 (PubMed:15489334).Curated	3
Sequence conflictⁱ	697 – 699	Missing in AAI44415 (PubMed:15489334).Curated	3

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_052959	466	S → Y. Corresponds to variant dbSNP:rs13246077Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_006812	269 – 294	Missing in isoform Short. 2 PublicationsAdd BLAST		26

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB046845 mRNA Translation: BAB13451.1 Different initiation. AC004893 Genomic DNA Translation: AAC62434.1 AF464850 mRNA Translation: AAM90910.1 AC073468 Genomic DNA No translation available. AC114500 Genomic DNA No translation available. CH236956 Genomic DNA Translation: EAL23885.1 CH236956 Genomic DNA Translation: EAL23886.1 CH471091 Genomic DNA Translation: EAW76687.1 CH471091 Genomic DNA Translation: EAW76688.1 BC136804 mRNA Translation: AAI36805.1 BC144414 mRNA Translation: AAI44415.1 BC152468 mRNA Translation: AAI52469.1 AF199364 mRNA Translation: AAF08298.2
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS34689.1 [Q9HCE7-2] CCDS34690.1 [Q9HCE7-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_001186776.1, NM_001199847.1 NP_065162.1, NM_020429.2 [Q9HCE7-1] NP_851994.1, NM_181349.2 [Q9HCE7-2]
UniGeneⁱ	Hs.189329

Genome annotation databases

Ensemblⁱ	ENST00000361125; ENSP00000354621; ENSG00000198742 [Q9HCE7-1] ENST00000361368; ENSP00000355326; ENSG00000198742 [Q9HCE7-2] ENST00000638461; ENSP00000491036; ENSG00000284126 [Q9HCE7-2] ENST00000638647; ENSP00000492201; ENSG00000284126 [Q9HCE7-1]
GeneIDⁱ	57154
KEGGⁱ	hsa:57154
UCSC genome browser More...UCSCⁱ	uc003upu.3 human [Q9HCE7-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9HCE7	E3 ubiquitin-protein ligase		PANTR		757	UniRef100_Q9HCE7
Q9HCE7-2	E3 ubiquitin-protein ligase		PANTR		731	UniRef100_Q9HCE7-2
UPI0000DC800A		MACFA		556

Protein	Similar proteins		Species	Score	Length	Source
Q9HCE7	E3 ubiquitin-protein ligase		PANTR		757	UniRef90_Q9HCE7
E3 ubiquitin-protein ligase		PONAB		838
E3 ubiquitin-protein ligase		PAPAN		757
E3 ubiquitin-protein ligase		MACFA		757
E3 ubiquitin-protein ligase		CALJA		757
+90
Q9HCE7-2	E3 ubiquitin-protein ligase SMURF1		MOUSE		731	UniRef90_Q9HCE7-2
E3 ubiquitin-protein ligase		PANTR		731
E3 ubiquitin-protein ligase		COLLI		742
E3 ubiquitin-protein ligase		MELGA		741
E3 ubiquitin-protein ligase		PHYCD		737
+310

Protein	Similar proteins		Species	Score	Length	Source
Q9HCE7	E3 ubiquitin-protein ligase		PANTR		757	UniRef50_Q9HCE7
E3 ubiquitin-protein ligase		PONAB		838
E3 ubiquitin-protein ligase		OTOGA		757
E3 ubiquitin-protein ligase		MOUSE		757
E3 ubiquitin-protein ligase		FELCA		757
+129
Q9HCE7-2	E3 ubiquitin-protein ligase SMURF1		MOUSE		731	UniRef50_Q9HCE7-2
E3 ubiquitin-protein ligase SMURF1		XENLA		731
E3 ubiquitin-protein ligase		PANTR		731
E3 ubiquitin-protein ligase		COLLI		742
E3 ubiquitin-protein ligase		MELGA		741
+667

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB046845 mRNA Translation: BAB13451.1 Different initiation. AC004893 Genomic DNA Translation: AAC62434.1 AF464850 mRNA Translation: AAM90910.1 AC073468 Genomic DNA No translation available. AC114500 Genomic DNA No translation available. CH236956 Genomic DNA Translation: EAL23885.1 CH236956 Genomic DNA Translation: EAL23886.1 CH471091 Genomic DNA Translation: EAW76687.1 CH471091 Genomic DNA Translation: EAW76688.1 BC136804 mRNA Translation: AAI36805.1 BC144414 mRNA Translation: AAI44415.1 BC152468 mRNA Translation: AAI52469.1 AF199364 mRNA Translation: AAF08298.2
CCDSⁱ	CCDS34689.1 [Q9HCE7-2] CCDS34690.1 [Q9HCE7-1]
RefSeqⁱ	NP_001186776.1, NM_001199847.1 NP_065162.1, NM_020429.2 [Q9HCE7-1] NP_851994.1, NM_181349.2 [Q9HCE7-2]
UniGeneⁱ	Hs.189329

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2LAZ	NMR	-	A	235-267	[»]
	2LB0	NMR	-	A	235-267	[»]
	2LB1	NMR	-	A	305-340	[»]
	2LTX	NMR	-	A	306-340	[»]
	3PYC	X-ray	1.96	A	13-140	[»]
ProteinModelPortalⁱ	Q9HCE7
SMRⁱ	Q9HCE7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	121411, 416 interactors
CORUMⁱ	Q9HCE7
DIPⁱ	DIP-36709N
IntActⁱ	Q9HCE7, 25 interactors
MINTⁱ	Q9HCE7
STRINGⁱ	9606.ENSP00000354621

Chemistry databases

ChEMBLⁱ	CHEMBL3879859

ChEMBLⁱ

CHEMBL3879859

PTM databases

iPTMnetⁱ	Q9HCE7
PhosphoSitePlusⁱ	Q9HCE7

Polymorphism and mutation databases

BioMutaⁱ	SMURF1
DMDMⁱ	17865625

Proteomic databases

EPDⁱ	Q9HCE7
MaxQBⁱ	Q9HCE7
PaxDbⁱ	Q9HCE7
PeptideAtlasⁱ	Q9HCE7
PRIDEⁱ	Q9HCE7
ProteomicsDBⁱ	81693 81694 [Q9HCE7-2]

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENST00000361125; ENSP00000354621; ENSG00000198742 [Q9HCE7-1] ENST00000361368; ENSP00000355326; ENSG00000198742 [Q9HCE7-2] ENST00000638461; ENSP00000491036; ENSG00000284126 [Q9HCE7-2] ENST00000638647; ENSP00000492201; ENSG00000284126 [Q9HCE7-1]
GeneIDⁱ	57154
KEGGⁱ	hsa:57154
UCSCⁱ	uc003upu.3 human [Q9HCE7-1]

Organism-specific databases

CTDⁱ	57154
DisGeNETⁱ	57154
EuPathDBⁱ	HostDB:ENSG00000198742.9
GeneCardsⁱ	SMURF1
HGNCⁱ	HGNC:16807 SMURF1
HPAⁱ	HPA055245
MIMⁱ	605568 gene
neXtProtⁱ	NX_Q9HCE7
OpenTargetsⁱ	ENSG00000198742
PharmGKBⁱ	PA134987175
HUGEⁱ	Search...
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0940 Eukaryota COG5021 LUCA
GeneTreeⁱ	ENSGT00760000118966
HOGENOMⁱ	HOG000208451
HOVERGENⁱ	HBG004134
InParanoidⁱ	Q9HCE7
KOⁱ	K04678
OMAⁱ	CKLNPTD
OrthoDBⁱ	EOG091G011S
PhylomeDBⁱ	Q9HCE7
TreeFamⁱ	TF323658

Enzyme and pathway databases

UniPathwayⁱ	UPA00143
BRENDAⁱ	2.3.2.B9 2681 6.3.2.19 2681
Reactomeⁱ	R-HSA-201451 Signaling by BMP R-HSA-2173788 Downregulation of TGF-beta receptor signaling R-HSA-2173791 TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) R-HSA-4608870 Asymmetric localization of PCP proteins R-HSA-5632684 Hedgehog 'on' state R-HSA-8939902 Regulation of RUNX2 expression and activity R-HSA-8941858 Regulation of RUNX3 expression and activity R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkⁱ	Q9HCE7
SIGNORⁱ	Q9HCE7

Miscellaneous databases

ChiTaRSⁱ	SMURF1 human
EvolutionaryTraceⁱ	Q9HCE7
GeneWikiⁱ	SMURF1
GenomeRNAiⁱ	57154
Protein Ontology More...PROⁱ	PR:Q9HCE7
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000198742 Expressed in 201 organ(s), highest expression level in testis
CleanExⁱ	HS_SMURF1
Genevisibleⁱ	Q9HCE7 HS

Family and domain databases

CDDⁱ	cd00078 HECTc, 1 hit cd00201 WW, 2 hits
Gene3Dⁱ	2.60.40.150, 1 hit
InterProⁱ	View protein in InterPro IPR000008 C2_dom IPR035892 C2_domain_sf IPR024928 E3_ub_ligase_SMURF1 IPR000569 HECT_dom IPR035983 Hect_E3_ubiquitin_ligase IPR001202 WW_dom IPR036020 WW_dom_sf
Pfamⁱ	View protein in Pfam PF00168 C2, 1 hit PF00632 HECT, 1 hit PF00397 WW, 2 hits
PIRSFⁱ	PIRSF001569 E3_ub_ligase_SMURF1, 1 hit
SMARTⁱ	View protein in SMART SM00239 C2, 1 hit SM00119 HECTc, 1 hit SM00456 WW, 2 hits
SUPFAMⁱ	SSF51045 SSF51045, 2 hits SSF56204 SSF56204, 1 hit
PROSITEⁱ	View protein in PROSITE PS50004 C2, 1 hit PS50237 HECT, 1 hit PS01159 WW_DOMAIN_1, 1 hit PS50020 WW_DOMAIN_2, 2 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SMUF1_HUMAN
Accessionⁱ	Q9HCE7Primary (citable) accession number: Q9HCE7 Secondary accession number(s): A4D279 , B7ZMB6, B9EGV3, O75853, Q547Q3, Q9UJT8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 13, 2001
	Last sequence update:	December 13, 2001
	Last modified:	November 7, 2018
	This is version 178 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PATHWAY comments
Index of metabolic and biosynthesis pathways
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9HCE7 (SMUF1_HUMAN)

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E3 ubiquitin-protein ligase SMURF1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Other locations

Cytosol

Extracellular region or secreted

Nucleus

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: protein ubiquitination

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ