UniProtKB - Q9HC98 (NEK6_HUMAN)
Serine/threonine-protein kinase Nek6
NEK6
Functioni
Protein kinase which plays an important role in mitotic cell cycle progression (PubMed:14563848).
Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis (PubMed:19414596).
Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3 (PubMed:12054534, PubMed:20873783).
Phosphorylates KIF11 to promote mitotic spindle formation (PubMed:19001501).
Involved in G2/M phase cell cycle arrest induced by DNA damage (PubMed:18728393).
Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence (PubMed:21099361).
Phosphorylates EML4 at 'Ser-144', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757).
8 PublicationsCaution
Catalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 74 | ATPPROSITE-ProRule annotation | 1 | |
Sitei | 108 | Autoinhibitory | 1 | |
Active sitei | 172 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 51 – 59 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- DNA-binding transcription factor binding Source: UniProtKB
- kinesin binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- protein kinase binding Source: UniProtKB
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: UniProtKB
- protein serine kinase activity Source: RHEA
- transcription corepressor binding Source: UniProtKB
- ubiquitin protein ligase binding Source: UniProtKB
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- chromosome segregation Source: UniProtKB
- mitotic nuclear membrane disassembly Source: Reactome
- mitotic spindle organization Source: Reactome
- peptidyl-serine phosphorylation Source: UniProtKB
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- protein autophosphorylation Source: UniProtKB
- protein phosphorylation Source: UniProtKB
- regulation of cellular senescence Source: UniProtKB
- regulation of mitotic cell cycle Source: UniProtKB
- regulation of mitotic metaphase/anaphase transition Source: UniProtKB
- spindle assembly Source: UniProtKB
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Apoptosis, Cell cycle, Cell division, Chromosome partition, Mitosis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9HC98 |
Reactomei | R-HSA-2980767, Activation of NIMA Kinases NEK9, NEK6, NEK7 R-HSA-3301854, Nuclear Pore Complex (NPC) Disassembly R-HSA-9648025, EML4 and NUDC in mitotic spindle formation |
SignaLinki | Q9HC98 |
SIGNORi | Q9HC98 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein kinase Nek6 (EC:2.7.11.1)Alternative name(s): Never in mitosis A-related kinase 6 Short name: NimA-related protein kinase 6 Protein kinase SID6-1512 |
Gene namesi | Name:NEK6 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7749, NEK6 |
MIMi | 604884, gene |
neXtProti | NX_Q9HC98 |
VEuPathDBi | HostDB:ENSG00000119408 |
Subcellular locationi
Nucleus
Cytoplasm and Cytosol
Cytoskeleton
Note: Colocalizes with APBB1 at the nuclear speckles. Colocalizes with PIN1 in the nucleus. Colocalizes with ATF4, CIR1, ARHGAP33, ANKRA2, CDC42, NEK9, RAD26L, RBBP6, RPS7, TRIP4, RELB and PHF1 in the centrosome. Localizes to spindle microtubules in metaphase and anaphase and to the midbody during cytokinesis.
Cytoskeleton
- centriolar satellite Source: HPA
- microtubule Source: UniProtKB-KW
- spindle pole Source: UniProtKB-SubCell
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear speck Source: UniProtKB-SubCell
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- protein-containing complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Microtubule, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 74 | K → M: Loss of autophosphorylation and of kinase activity and induction of apoptosis; when associated with M-75. 2 Publications | 1 | |
Mutagenesisi | 75 | K → M: Loss of autophosphorylation and of kinase activity and induction of apoptosis; when associated with M-74. 2 Publications | 1 | |
Mutagenesisi | 108 | Y → A: Increase in catalytic activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 10783 |
OpenTargetsi | ENSG00000119408 |
PharmGKBi | PA31550 |
Miscellaneous databases
Pharosi | Q9HC98, Tchem |
Chemistry databases
ChEMBLi | CHEMBL4309 |
DrugCentrali | Q9HC98 |
Genetic variation databases
BioMutai | NEK6 |
DMDMi | 37537993 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086427 | 1 – 313 | Serine/threonine-protein kinase Nek6Add BLAST | 313 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 37 | Phosphoserine1 Publication | 1 | |
Modified residuei | 202 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 206 | Phosphoserine; by NEK9Combined sources2 Publications | 1 | |
Modified residuei | 210 | Phosphothreonine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9HC98 |
jPOSTi | Q9HC98 |
MassIVEi | Q9HC98 |
MaxQBi | Q9HC98 |
PaxDbi | Q9HC98 |
PeptideAtlasi | Q9HC98 |
PRIDEi | Q9HC98 |
ProteomicsDBi | 81659 [Q9HC98-1] 81660 [Q9HC98-2] 81661 [Q9HC98-3] 81662 [Q9HC98-4] |
PTM databases
iPTMneti | Q9HC98 |
PhosphoSitePlusi | Q9HC98 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000119408, Expressed in sural nerve and 192 other tissues |
ExpressionAtlasi | Q9HC98, baseline and differential |
Genevisiblei | Q9HC98, HS |
Organism-specific databases
HPAi | ENSG00000119408, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with STAT3 and RPS6KB1 (By similarity).
Interacts with NEK9, predominantly in mitosis.
Interacts with KIF11 (via C-terminus).
Interacts with APBB1 (via WW domain).
Interacts with ANKRA2, ATF4, ARHGAP33, CDC42, CIR1, PRAM1, PTN, PRDX3, PIN1, RAD26L, RBBP6, RPS7 and TRIP4.
By similarity5 PublicationsBinary interactionsi
Q9HC98
Isoform 4 [Q9HC98-4]
GO - Molecular functioni
- DNA-binding transcription factor binding Source: UniProtKB
- kinesin binding Source: UniProtKB
- protein kinase binding Source: UniProtKB
- transcription corepressor binding Source: UniProtKB
- ubiquitin protein ligase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 116000, 151 interactors |
IntActi | Q9HC98, 112 interactors |
MINTi | Q9HC98 |
STRINGi | 9606.ENSP00000362702 |
Chemistry databases
BindingDBi | Q9HC98 |
Miscellaneous databases
RNActi | Q9HC98, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 45 – 310 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 266 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 44 | Interaction with ARHGAP33, ANKRA2, CDC42, PRDX3, RAD26L, RBBP6, RPS7 and TRIP41 PublicationAdd BLAST | 44 | |
Regioni | 1 – 32 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 267 – 270 | Interaction with APBB11 Publication | 4 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0591, Eukaryota |
GeneTreei | ENSGT00940000159990 |
InParanoidi | Q9HC98 |
OrthoDBi | 1290401at2759 |
PhylomeDBi | Q9HC98 |
TreeFami | TF101021 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PRINTSi | PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGQPGHMPH GGSSNNLCHT LGPVHPPDPQ RHPNTLSFRC SLADFQIEKK
60 70 80 90 100
IGRGQFSEVY KATCLLDRKT VALKKVQIFE MMDAKARQDC VKEIGLLKQL
110 120 130 140 150
NHPNIIKYLD SFIEDNELNI VLELADAGDL SQMIKYFKKQ KRLIPERTVW
160 170 180 190 200
KYFVQLCSAV EHMHSRRVMH RDIKPANVFI TATGVVKLGD LGLGRFFSSE
210 220 230 240 250
TTAAHSLVGT PYYMSPERIH ENGYNFKSDI WSLGCLLYEM AALQSPFYGD
260 270 280 290 300
KMNLFSLCQK IEQCDYPPLP GEHYSEKLRE LVSMCICPDP HQRPDIGYVH
310
QVAKQMHIWM SST
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5TBG1 | Q5TBG1_HUMAN | Serine/threonine-protein kinase Nek... | NEK6 | 207 | Annotation score: | ||
Q5TBG7 | Q5TBG7_HUMAN | Serine/threonine-protein kinase Nek... | NEK6 | 219 | Annotation score: | ||
Q5TBH1 | Q5TBH1_HUMAN | Serine/threonine-protein kinase Nek... | NEK6 | 222 | Annotation score: | ||
Q5TBH2 | Q5TBH2_HUMAN | Serine/threonine-protein kinase Nek... | NEK6 | 220 | Annotation score: | ||
Q5TBG2 | Q5TBG2_HUMAN | Serine/threonine-protein kinase Nek... | NEK6 | 171 | Annotation score: | ||
Q5TBG4 | Q5TBG4_HUMAN | Serine/threonine-protein kinase Nek... | NEK6 | 194 | Annotation score: | ||
Q5TBH0 | Q5TBH0_HUMAN | Serine/threonine-protein kinase Nek... | NEK6 | 200 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 88 | Q → W in CAG33372 (Ref. 7) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041798 | 1 | M → MPRREVCWEAAHFRQEEQSL PRPRVRALVRLACRM in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_041799 | 1 | M → MGRRRPAPFRALVRLACRM in isoform 3. 1 Publication | 1 | |
Alternative sequenceiVSP_041800 | 1 | M → MEATGWDSRCSPGTQVRALV RLACRM in isoform 4. 1 Publication | 1 |
Sequence databases
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
AlphaFoldDBi | Q9HC98 |
SMRi | Q9HC98 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 116000, 151 interactors |
IntActi | Q9HC98, 112 interactors |
MINTi | Q9HC98 |
STRINGi | 9606.ENSP00000362702 |
Chemistry databases
BindingDBi | Q9HC98 |
ChEMBLi | CHEMBL4309 |
DrugCentrali | Q9HC98 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
PTM databases
iPTMneti | Q9HC98 |
PhosphoSitePlusi | Q9HC98 |
Genetic variation databases
BioMutai | NEK6 |
DMDMi | 37537993 |
Proteomic databases
EPDi | Q9HC98 |
jPOSTi | Q9HC98 |
MassIVEi | Q9HC98 |
MaxQBi | Q9HC98 |
PaxDbi | Q9HC98 |
PeptideAtlasi | Q9HC98 |
PRIDEi | Q9HC98 |
ProteomicsDBi | 81659 [Q9HC98-1] 81660 [Q9HC98-2] 81661 [Q9HC98-3] 81662 [Q9HC98-4] |
Protocols and materials databases
Antibodypediai | 30447, 299 antibodies from 32 providers |
DNASUi | 10783 |
Genome annotation databases
Organism-specific databases
CTDi | 10783 |
DisGeNETi | 10783 |
GeneCardsi | NEK6 |
HGNCi | HGNC:7749, NEK6 |
HPAi | ENSG00000119408, Low tissue specificity |
MIMi | 604884, gene |
neXtProti | NX_Q9HC98 |
OpenTargetsi | ENSG00000119408 |
PharmGKBi | PA31550 |
VEuPathDBi | HostDB:ENSG00000119408 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0591, Eukaryota |
GeneTreei | ENSGT00940000159990 |
InParanoidi | Q9HC98 |
OrthoDBi | 1290401at2759 |
PhylomeDBi | Q9HC98 |
TreeFami | TF101021 |
Enzyme and pathway databases
PathwayCommonsi | Q9HC98 |
Reactomei | R-HSA-2980767, Activation of NIMA Kinases NEK9, NEK6, NEK7 R-HSA-3301854, Nuclear Pore Complex (NPC) Disassembly R-HSA-9648025, EML4 and NUDC in mitotic spindle formation |
SignaLinki | Q9HC98 |
SIGNORi | Q9HC98 |
Miscellaneous databases
BioGRID-ORCSi | 10783, 22 hits in 1115 CRISPR screens |
ChiTaRSi | NEK6, human |
GeneWikii | NEK6 |
GenomeRNAii | 10783 |
Pharosi | Q9HC98, Tchem |
PROi | PR:Q9HC98 |
RNActi | Q9HC98, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000119408, Expressed in sural nerve and 192 other tissues |
ExpressionAtlasi | Q9HC98, baseline and differential |
Genevisiblei | Q9HC98, HS |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PRINTSi | PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NEK6_HUMAN | |
Accessioni | Q9HC98Primary (citable) accession number: Q9HC98 Secondary accession number(s): B7Z2D9 Q9ULX2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2003 |
Last sequence update: | October 3, 2003 | |
Last modified: | May 25, 2022 | |
This is version 190 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families