NEK6

Functionⁱ

Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence.8 Publications

Catalytic activityⁱ

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactorⁱ

Mg²⁺

Activity regulationⁱ

Binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-108.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	74	ATPPROSITE-ProRule annotation	1
Siteⁱ	108	Autoinhibitory	1
Active siteⁱ	172	Proton acceptorPROSITE-ProRule annotation	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	51 – 59	ATPPROSITE-ProRule annotation		9

GO - Molecular functionⁱ

activating transcription factor binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 20873783
ATP binding
Source: UniProtKB
Inferred from direct assayⁱ
- 14563848
kinesin binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 19001501
magnesium ion binding
Source: UniProtKB
Inferred from direct assayⁱ
- 14563848
protein kinase binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 19001501
protein serine/threonine kinase activity
Source: UniProtKB
Inferred from direct assayⁱ
transcription corepressor binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 20873783
ubiquitin protein ligase binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 20873783

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

apoptotic process Source: UniProtKB-KW
cell division Source: UniProtKB-KW
chromosome segregation
Source: UniProtKB
Inferred from direct assayⁱ
- 14563848
mitotic nuclear envelope disassembly Source: Reactome
peptidyl-serine phosphorylation
Source: UniProtKB
Inferred from direct assayⁱ
- 19001501
positive regulation of I-kappaB kinase/NF-kappaB signaling
Source: UniProtKB
Inferred from high throughput mutant phenotypeⁱ
- 12761501
protein autophosphorylation
Source: UniProtKB
Inferred from direct assayⁱ
- 14563848
- 20873783
protein phosphorylation
Source: UniProtKB
Inferred from direct assayⁱ
- 12840024
regulation of cellular senescence
Source: UniProtKB
Traceable author statementⁱ
- 21200151
regulation of mitotic cell cycle
Source: UniProtKB
Traceable author statementⁱ
- 16280549
regulation of mitotic metaphase/anaphase transition
Source: UniProtKB
Inferred from direct assayⁱ
- 14563848
spindle assembly
Source: UniProtKB
Traceable author statementⁱ
- 21200151

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Serine/threonine-protein kinase, Transferase
Biological process	Apoptosis, Cell cycle, Cell division, Chromosome partition, Mitosis
Ligand	ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-HSA-2980767 Activation of NIMA Kinases NEK9, NEK6, NEK7 R-HSA-3301854 Nuclear Pore Complex (NPC) Disassembly
SignaLinkⁱ	Q9HC98
SIGNORⁱ	Q9HC98

Protein family/group databases

TCDBⁱ	1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

TCDBⁱ

1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Serine/threonine-protein kinase Nek6 (EC:2.7.11.1) Alternative name(s): Never in mitosis A-related kinase 6 Short name: NimA-related protein kinase 6 Protein kinase SID6-1512
Gene namesⁱ	Name:NEK6
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 9

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000119408.16
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:7749 NEK6
MIMⁱ	604884 gene
neXtProtⁱ	NX_Q9HC98

Keywords - Cellular componentⁱ

Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	74	K → M: Loss of autophosphorylation and of kinase activity and induction of apoptosis; when associated with M-75. 2 Publications	1
Mutagenesisⁱ	75	K → M: Loss of autophosphorylation and of kinase activity and induction of apoptosis; when associated with M-74. 2 Publications	1
Mutagenesisⁱ	108	Y → A: Increase in catalytic activity. 1 Publication	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	10783
Open Targets More...OpenTargetsⁱ	ENSG00000119408
PharmGKBⁱ	PA31550

Chemistry databases

ChEMBLⁱ	CHEMBL4309

ChEMBLⁱ

CHEMBL4309

Polymorphism and mutation databases

BioMutaⁱ	NEK6
DMDMⁱ	37537993

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000086427	1 – 313	Serine/threonine-protein kinase Nek6Add BLAST		313

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	37	Phosphoserine1 Publication	1
Modified residueⁱ	202	Phosphothreonine1 Publication	1
Modified residueⁱ	206	Phosphoserine; by NEK9Combined sources2 Publications	1
Modified residueⁱ	210	Phosphothreonine1 Publication	1

Post-translational modificationⁱ

Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or UV-induced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation at Thr-210.4 Publications

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	Q9HC98
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9HC98
PaxDbⁱ	Q9HC98
PeptideAtlas More...PeptideAtlasⁱ	Q9HC98
PRIDEⁱ	Q9HC98
ProteomicsDBⁱ	81659 81660 [Q9HC98-2] 81661 [Q9HC98-3] 81662 [Q9HC98-4]

PTM databases

iPTMnetⁱ	Q9HC98
PhosphoSitePlusⁱ	Q9HC98

Expressionⁱ

Tissue specificityⁱ

Ubiquitous, with highest expression in heart and skeletal muscle. Up-regulated in a variety of malignant cancers, such as breast, colon, lung, and gastric cancers.2 Publications

Inductionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000119408 Expressed in 176 organ(s), highest expression level in pigmented layer of retina
CleanExⁱ	HS_NEK6
ExpressionAtlasⁱ	Q9HC98 baseline and differential
Genevisibleⁱ	Q9HC98 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB045993 HPA056828

HPAⁱ

CAB045993
HPA056828

Interactionⁱ

Subunit structureⁱ

Interacts with STAT3 and RPS6KB1 (By similarity). Interacts with NEK9, predominantly in mitosis. Interacts with KIF11 (via C-terminus). Interacts with APBB1 (via WW domain). Interacts with ANKRA2, ATF4, ARHGAP33, CDC42, CIR1, PRAM1, PTN, PRDX3, PIN1, RAD26L, RBBP6, RPS7 and TRIP4.By similarity5 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
AES	Q08117	3	EBI-740364,EBI-717810
CCDC36	Q8IYA8	3	EBI-740364,EBI-8638439
FAM208B	Q5VWN6-2	3	EBI-740364,EBI-10172380
HSD17B14	Q9BPX1	3	EBI-740364,EBI-742664
IKZF1	Q13422	3	EBI-740364,EBI-745305
IKZF3	Q9UKT9	3	EBI-740364,EBI-747204
INCA1	Q0VD86	3	EBI-740364,EBI-6509505
KRT31	Q15323	3	EBI-740364,EBI-948001
KRT40	Q6A162	3	EBI-740364,EBI-10171697
LNX1	Q8TBB1	3	EBI-740364,EBI-739832
LZTS2	Q9BRK4	3	EBI-740364,EBI-741037
NECAB2	Q7Z6G3-2	3	EBI-740364,EBI-10172876
PIN1	Q13526	3	EBI-740364,EBI-714158
TCF4	P15884	3	EBI-740364,EBI-533224
TNK2	Q07912	3	EBI-740364,EBI-603457
TRIM23	P36406	3	EBI-740364,EBI-740098
TRIM27	P14373	3	EBI-740364,EBI-719493
XIAP	P98170	3	EBI-740364,EBI-517127

GO - Molecular functionⁱ

activating transcription factor binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 20873783
kinesin binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 19001501
protein kinase binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 19001501
transcription corepressor binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 20873783
ubiquitin protein ligase binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 20873783

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	116000, 68 interactors
IntActⁱ	Q9HC98, 94 interactors
Molecular INTeraction database More...MINTⁱ	Q9HC98
STRINGⁱ	9606.ENSP00000362702

Chemistry databases

BindingDBⁱ

Q9HC98

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9HC98
SMRⁱ	Q9HC98
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	45 – 310	Protein kinasePROSITE-ProRule annotationAdd BLAST		266

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	1 – 44	Interaction with ARHGAP33, ANKRA2, CDC42, PRDX3, RAD26L, RBBP6, RPS7 and TRIP41 PublicationAdd BLAST		44
Regionⁱ	267 – 270	Interaction with APBB11 Publication		4

Domainⁱ

Displays an autoinhibited conformation: Tyr-108 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9.1 Publication

Sequence similaritiesⁱ

Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.Curated

Phylogenomic databases

eggNOGⁱ	KOG0591 Eukaryota ENOG410XNQP LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118997
HOVERGENⁱ	HBG105886
InParanoidⁱ	Q9HC98
KEGG Orthology (KO) More...KOⁱ	K20875
OMAⁱ	HQIAKQM
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0AER
PhylomeDBⁱ	Q9HC98
TreeFamⁱ	TF101021

Family and domain databases

InterProⁱ	View protein in InterPro IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR001245 Ser-Thr/Tyr_kinase_cat_dom IPR008271 Ser/Thr_kinase_AS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00069 Pkinase, 1 hit
PRINTSⁱ	PR00109 TYRKINASE
SMARTⁱ	View protein in SMART SM00220 S_TKc, 1 hit
SUPFAMⁱ	SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (4+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 4 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 4 described isoforms and 7 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q9HC98-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGQPGHMPH GGSSNNLCHT LGPVHPPDPQ RHPNTLSFRC SLADFQIEKK 
        60         70         80         90        100
IGRGQFSEVY KATCLLDRKT VALKKVQIFE MMDAKARQDC VKEIGLLKQL 
       110        120        130        140        150
NHPNIIKYLD SFIEDNELNI VLELADAGDL SQMIKYFKKQ KRLIPERTVW 
       160        170        180        190        200
KYFVQLCSAV EHMHSRRVMH RDIKPANVFI TATGVVKLGD LGLGRFFSSE 
       210        220        230        240        250
TTAAHSLVGT PYYMSPERIH ENGYNFKSDI WSLGCLLYEM AALQSPFYGD 
       260        270        280        290        300
KMNLFSLCQK IEQCDYPPLP GEHYSEKLRE LVSMCICPDP HQRPDIGYVH 
       310 
QVAKQMHIWM SST

Length:313

Mass (Da):35,714

Last modified:October 3, 2003 - v2

Checksum:ⁱB898DC57407A8C16

GO

Isoform 2 (identifier: Q9HC98-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-1: M → MPRREVCWEAAHFRQEEQSLPRPRVRALVRLACRM

« Hide

        10         20         30         40         50
MPRREVCWEA AHFRQEEQSL PRPRVRALVR LACRMAGQPG HMPHGGSSNN 
        60         70         80         90        100
LCHTLGPVHP PDPQRHPNTL SFRCSLADFQ IEKKIGRGQF SEVYKATCLL 
       110        120        130        140        150
DRKTVALKKV QIFEMMDAKA RQDCVKEIGL LKQLNHPNII KYLDSFIEDN 
       160        170        180        190        200
ELNIVLELAD AGDLSQMIKY FKKQKRLIPE RTVWKYFVQL CSAVEHMHSR 
       210        220        230        240        250
RVMHRDIKPA NVFITATGVV KLGDLGLGRF FSSETTAAHS LVGTPYYMSP 
       260        270        280        290        300
ERIHENGYNF KSDIWSLGCL LYEMAALQSP FYGDKMNLFS LCQKIEQCDY 
       310        320        330        340 
PPLPGEHYSE KLRELVSMCI CPDPHQRPDI GYVHQVAKQM HIWMSST

Show »

Length:347

Mass (Da):39,844

Checksum:ⁱ2CA3B83E91024B8A

GO

Isoform 3 (identifier: Q9HC98-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-1: M → MGRRRPAPFRALVRLACRM

« Hide

        10         20         30         40         50
MGRRRPAPFR ALVRLACRMA GQPGHMPHGG SSNNLCHTLG PVHPPDPQRH 
        60         70         80         90        100
PNTLSFRCSL ADFQIEKKIG RGQFSEVYKA TCLLDRKTVA LKKVQIFEMM 
       110        120        130        140        150
DAKARQDCVK EIGLLKQLNH PNIIKYLDSF IEDNELNIVL ELADAGDLSQ 
       160        170        180        190        200
MIKYFKKQKR LIPERTVWKY FVQLCSAVEH MHSRRVMHRD IKPANVFITA 
       210        220        230        240        250
TGVVKLGDLG LGRFFSSETT AAHSLVGTPY YMSPERIHEN GYNFKSDIWS 
       260        270        280        290        300
LGCLLYEMAA LQSPFYGDKM NLFSLCQKIE QCDYPPLPGE HYSEKLRELV 
       310        320        330 
SMCICPDPHQ RPDIGYVHQV AKQMHIWMSS T

Show »

Length:331

Mass (Da):37,823

Checksum:ⁱDF83ECFFE6F5B2F6

GO

Isoform 4 (identifier: Q9HC98-4) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-1: M → MEATGWDSRCSPGTQVRALVRLACRM

« Hide

        10         20         30         40         50
MEATGWDSRC SPGTQVRALV RLACRMAGQP GHMPHGGSSN NLCHTLGPVH 
        60         70         80         90        100
PPDPQRHPNT LSFRCSLADF QIEKKIGRGQ FSEVYKATCL LDRKTVALKK 
       110        120        130        140        150
VQIFEMMDAK ARQDCVKEIG LLKQLNHPNI IKYLDSFIED NELNIVLELA 
       160        170        180        190        200
DAGDLSQMIK YFKKQKRLIP ERTVWKYFVQ LCSAVEHMHS RRVMHRDIKP 
       210        220        230        240        250
ANVFITATGV VKLGDLGLGR FFSSETTAAH SLVGTPYYMS PERIHENGYN 
       260        270        280        290        300
FKSDIWSLGC LLYEMAALQS PFYGDKMNLF SLCQKIEQCD YPPLPGEHYS 
       310        320        330 
EKLRELVSMC ICPDPHQRPD IGYVHQVAKQ MHIWMSST

Show »

Length:338

Mass (Da):38,460

Checksum:ⁱ2B680899AE6D5765

GO

Computationally mapped potential isoform sequencesⁱ

There are 7 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

Q5TBG7	Q5TBG7_HUMAN	Serine/threonine-protein kinase Nek... Serine/threonine-protein kinase Nek6	NEK6	219	Annotation score:
Q5TBH2	Q5TBH2_HUMAN	Serine/threonine-protein kinase Nek... Serine/threonine-protein kinase Nek6	NEK6	220	Annotation score:
Q5TBH1	Q5TBH1_HUMAN	Serine/threonine-protein kinase Nek... Serine/threonine-protein kinase Nek6	NEK6	222	Annotation score:
Q5TBG1	Q5TBG1_HUMAN	Serine/threonine-protein kinase Nek... Serine/threonine-protein kinase Nek6	NEK6	207	Annotation score:
Q5TBG4	Q5TBG4_HUMAN	Serine/threonine-protein kinase Nek... Serine/threonine-protein kinase Nek6	NEK6	194	Annotation score:
Q5TBG2	Q5TBG2_HUMAN	Serine/threonine-protein kinase Nek... Serine/threonine-protein kinase Nek6	NEK6	171	Annotation score:
Q5TBH0	Q5TBH0_HUMAN	Serine/threonine-protein kinase Nek... Serine/threonine-protein kinase Nek6	NEK6	200	Annotation score:

Sequence cautionⁱ

The sequence AAG13417 differs from that shown. Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated

The sequence AAH00101 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

The sequence AAH04174 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

The sequence AAH04209 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

The sequence BAA85045 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	88	Q → W in CAG33372 (Ref. 7) Curated		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_041798	1	M → MPRREVCWEAAHFRQEEQSL PRPRVRALVRLACRM in isoform 2. 1 Publication	1
Alternative sequenceⁱVSP_041799	1	M → MGRRRPAPFRALVRLACRM in isoform 3. 1 Publication	1
Alternative sequenceⁱVSP_041800	1	M → MEATGWDSRCSPGTQVRALV RLACRM in isoform 4. 1 Publication	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF087909 mRNA Translation: AAG13417.1 Sequence problems. AB026289 mRNA Translation: BAA85045.1 Different initiation. AK294614 mRNA Translation: BAH11825.1 AK313071 mRNA Translation: BAG35899.1 AL162724 Genomic DNA No translation available. AL137846 Genomic DNA No translation available. CH471090 Genomic DNA Translation: EAW87579.1 CH471090 Genomic DNA Translation: EAW87581.1 BC000101 mRNA Translation: AAH00101.2 Different initiation. BC004174 mRNA Translation: AAH04174.2 Different initiation. BC004209 mRNA Translation: AAH04209.2 Different initiation. BC012761 mRNA Translation: AAH12761.1 CR457091 mRNA Translation: CAG33372.1 CR542222 mRNA Translation: CAG47018.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS48015.1 [Q9HC98-2] CCDS55338.1 [Q9HC98-3] CCDS55339.1 [Q9HC98-4] CCDS6854.1 [Q9HC98-1]
PIRⁱ	JC7838
NCBI Reference Sequences More...RefSeqⁱ	NP_001138473.1, NM_001145001.2 [Q9HC98-2] NP_001159639.1, NM_001166167.1 [Q9HC98-3] NP_001159640.1, NM_001166168.1 [Q9HC98-1] NP_001159641.1, NM_001166169.1 [Q9HC98-4] NP_001159642.1, NM_001166170.1 [Q9HC98-1] NP_001159643.1, NM_001166171.1 [Q9HC98-2] NP_055212.2, NM_014397.5 [Q9HC98-1] XP_005251721.1, XM_005251664.1 [Q9HC98-1] XP_006716999.1, XM_006716936.2 [Q9HC98-1] XP_016869706.1, XM_017014217.1 [Q9HC98-2]
UniGeneⁱ	Hs.197071

Genome annotation databases

Ensemblⁱ	ENST00000320246; ENSP00000319734; ENSG00000119408 [Q9HC98-1] ENST00000373600; ENSP00000362702; ENSG00000119408 [Q9HC98-2] ENST00000373603; ENSP00000362705; ENSG00000119408 [Q9HC98-1] ENST00000394199; ENSP00000377749; ENSG00000119408 [Q9HC98-2] ENST00000539416; ENSP00000439651; ENSG00000119408 [Q9HC98-4] ENST00000540326; ENSP00000441469; ENSG00000119408 [Q9HC98-3] ENST00000545174; ENSP00000442636; ENSG00000119408 [Q9HC98-1] ENST00000546191; ENSP00000441426; ENSG00000119408 [Q9HC98-1]
GeneIDⁱ	10783
KEGGⁱ	hsa:10783
UCSC genome browser More...UCSCⁱ	uc004bof.4 human [Q9HC98-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9HC98	Serine/threonine-protein kinase Nek6 (Fragment)		HUMAN		222	UniRef100_Q9HC98
NEK6 isoform 14 (Fragment)		PANTR		219
Serine/threonine-protein kinase Nek6 (Fragment)		HUMAN		219
NEK6 isoform 12 (Fragment)		PANTR		222
NIMA (Never in mitosis gene a)-related kinase 6, isoform CRA_b		HUMAN		313
+6

Protein	Similar proteins		Species	Score	Length	Source
Q9HC98	Serine/threonine-protein kinase Nek6 (Fragment)		HUMAN		222	UniRef90_Q9HC98
NEK6 isoform 14 (Fragment)		PANTR		219
Uncharacterized protein		PANPA		322
Uncharacterized protein		NOMLE		313
NIMA related kinase 6		GORGO		313
+12
Q9HC98-2	Serine/threonine-protein kinase Nek6		RAT		313	UniRef90_Q9HC98-2
Serine/threonine-protein kinase Nek6		PIG		313
Uncharacterized protein		FELCA		383
Uncharacterized protein		CALJA		351
NIMA related kinase 6		SAIBB		313
+165
Q9HC98-3	Isoform 2 of Serine/threonine-protein kinase Nek6		HUMAN		347	UniRef90_Q9HC98-2
Serine/threonine-protein kinase Nek6		RAT		313
Serine/threonine-protein kinase Nek6		PIG		313
Uncharacterized protein		FELCA		383
Uncharacterized protein		CALJA		351
+165
Q9HC98-4	Isoform 2 of Serine/threonine-protein kinase Nek6		HUMAN		347	UniRef90_Q9HC98-2
Serine/threonine-protein kinase Nek6		RAT		313
Serine/threonine-protein kinase Nek6		PIG		313
Uncharacterized protein		FELCA		383
Uncharacterized protein		CALJA		351
+165

Protein	Similar proteins		Species	Score	Length	Source
Q9HC98	Serine/threonine-protein kinase Nek6 (Fragment)		HUMAN		222	UniRef50_Q9HC98
NEK6 isoform 14 (Fragment)		PANTR		219
Uncharacterized protein		PANPA		322
Uncharacterized protein		NOMLE		313
NIMA related kinase 6		GORGO		313
+16
Q9HC98-2	Serine/threonine-protein kinase Nek6		PIG		313	UniRef50_Q9HC98-2
Serine/threonine-protein kinase Nek6		RAT		313
Serine/threonine-protein kinase Nek7	)	HUMAN		302
Uncharacterized protein		FELCA		383
Uncharacterized protein		CALJA		351
+353
Q9HC98-3	Isoform 2 of Serine/threonine-protein kinase Nek6		HUMAN		347	UniRef50_Q9HC98-2
Serine/threonine-protein kinase Nek6		PIG		313
Serine/threonine-protein kinase Nek6		RAT		313
Uncharacterized protein		FELCA		383
Uncharacterized protein		CALJA		351
+353
Q9HC98-4	Isoform 2 of Serine/threonine-protein kinase Nek6		HUMAN		347	UniRef50_Q9HC98-2
Serine/threonine-protein kinase Nek6		PIG		313
Serine/threonine-protein kinase Nek6		RAT		313
Uncharacterized protein		FELCA		383
Uncharacterized protein		CALJA		351
+353

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF087909 mRNA Translation: AAG13417.1 Sequence problems. AB026289 mRNA Translation: BAA85045.1 Different initiation. AK294614 mRNA Translation: BAH11825.1 AK313071 mRNA Translation: BAG35899.1 AL162724 Genomic DNA No translation available. AL137846 Genomic DNA No translation available. CH471090 Genomic DNA Translation: EAW87579.1 CH471090 Genomic DNA Translation: EAW87581.1 BC000101 mRNA Translation: AAH00101.2 Different initiation. BC004174 mRNA Translation: AAH04174.2 Different initiation. BC004209 mRNA Translation: AAH04209.2 Different initiation. BC012761 mRNA Translation: AAH12761.1 CR457091 mRNA Translation: CAG33372.1 CR542222 mRNA Translation: CAG47018.1
CCDSⁱ	CCDS48015.1 [Q9HC98-2] CCDS55338.1 [Q9HC98-3] CCDS55339.1 [Q9HC98-4] CCDS6854.1 [Q9HC98-1]
PIRⁱ	JC7838
RefSeqⁱ	NP_001138473.1, NM_001145001.2 [Q9HC98-2] NP_001159639.1, NM_001166167.1 [Q9HC98-3] NP_001159640.1, NM_001166168.1 [Q9HC98-1] NP_001159641.1, NM_001166169.1 [Q9HC98-4] NP_001159642.1, NM_001166170.1 [Q9HC98-1] NP_001159643.1, NM_001166171.1 [Q9HC98-2] NP_055212.2, NM_014397.5 [Q9HC98-1] XP_005251721.1, XM_005251664.1 [Q9HC98-1] XP_006716999.1, XM_006716936.2 [Q9HC98-1] XP_016869706.1, XM_017014217.1 [Q9HC98-2]
UniGeneⁱ	Hs.197071

3D structure databases

ProteinModelPortalⁱ	Q9HC98
SMRⁱ	Q9HC98
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	116000, 68 interactors
IntActⁱ	Q9HC98, 94 interactors
MINTⁱ	Q9HC98
STRINGⁱ	9606.ENSP00000362702

Chemistry databases

BindingDBⁱ	Q9HC98
ChEMBLⁱ	CHEMBL4309

Protein family/group databases

TCDBⁱ	1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

TCDBⁱ

1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

PTM databases

iPTMnetⁱ	Q9HC98
PhosphoSitePlusⁱ	Q9HC98

Polymorphism and mutation databases

BioMutaⁱ	NEK6
DMDMⁱ	37537993

Proteomic databases

EPDⁱ	Q9HC98
MaxQBⁱ	Q9HC98
PaxDbⁱ	Q9HC98
PeptideAtlasⁱ	Q9HC98
PRIDEⁱ	Q9HC98
ProteomicsDBⁱ	81659 81660 [Q9HC98-2] 81661 [Q9HC98-3] 81662 [Q9HC98-4]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	10783
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000320246; ENSP00000319734; ENSG00000119408 [Q9HC98-1] ENST00000373600; ENSP00000362702; ENSG00000119408 [Q9HC98-2] ENST00000373603; ENSP00000362705; ENSG00000119408 [Q9HC98-1] ENST00000394199; ENSP00000377749; ENSG00000119408 [Q9HC98-2] ENST00000539416; ENSP00000439651; ENSG00000119408 [Q9HC98-4] ENST00000540326; ENSP00000441469; ENSG00000119408 [Q9HC98-3] ENST00000545174; ENSP00000442636; ENSG00000119408 [Q9HC98-1] ENST00000546191; ENSP00000441426; ENSG00000119408 [Q9HC98-1]
GeneIDⁱ	10783
KEGGⁱ	hsa:10783
UCSCⁱ	uc004bof.4 human [Q9HC98-1]

Organism-specific databases

CTDⁱ	10783
DisGeNETⁱ	10783
EuPathDBⁱ	HostDB:ENSG00000119408.16
GeneCardsⁱ	NEK6
HGNCⁱ	HGNC:7749 NEK6
HPAⁱ	CAB045993 HPA056828
MIMⁱ	604884 gene
neXtProtⁱ	NX_Q9HC98
OpenTargetsⁱ	ENSG00000119408
PharmGKBⁱ	PA31550
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0591 Eukaryota ENOG410XNQP LUCA
GeneTreeⁱ	ENSGT00760000118997
HOVERGENⁱ	HBG105886
InParanoidⁱ	Q9HC98
KOⁱ	K20875
OMAⁱ	HQIAKQM
OrthoDBⁱ	EOG091G0AER
PhylomeDBⁱ	Q9HC98
TreeFamⁱ	TF101021

Enzyme and pathway databases

Reactomeⁱ	R-HSA-2980767 Activation of NIMA Kinases NEK9, NEK6, NEK7 R-HSA-3301854 Nuclear Pore Complex (NPC) Disassembly
SignaLinkⁱ	Q9HC98
SIGNORⁱ	Q9HC98

Miscellaneous databases

ChiTaRSⁱ	NEK6 human
GeneWikiⁱ	NEK6
GenomeRNAiⁱ	10783
Protein Ontology More...PROⁱ	PR:Q9HC98
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000119408 Expressed in 176 organ(s), highest expression level in pigmented layer of retina
CleanExⁱ	HS_NEK6
ExpressionAtlasⁱ	Q9HC98 baseline and differential
Genevisibleⁱ	Q9HC98 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR001245 Ser-Thr/Tyr_kinase_cat_dom IPR008271 Ser/Thr_kinase_AS
Pfamⁱ	View protein in Pfam PF00069 Pkinase, 1 hit
PRINTSⁱ	PR00109 TYRKINASE
SMARTⁱ	View protein in SMART SM00220 S_TKc, 1 hit
SUPFAMⁱ	SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NEK6_HUMAN
Accessionⁱ	Q9HC98Primary (citable) accession number: Q9HC98 Secondary accession number(s): B7Z2D9 Q9ULX2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 3, 2003
	Last sequence update:	October 3, 2003
	Last modified:	November 7, 2018
	This is version 168 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM
Human and mouse protein kinases
Human and mouse protein kinases: classification and index

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9HC98 (NEK6_HUMAN)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Serine/threonine-protein kinase Nek6

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Cytoskeleton

Nucleus

Other locations

Cytoskeleton

Cytosol

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ