UniProtKB - Q9HC97 (GPR35_HUMAN)
Protein
G-protein coupled receptor 35
Gene
GPR35
Organism
Homo sapiens (Human)
Status
Functioni
Acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of this receptor is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins.1 Publication
GO - Molecular functioni
- C-X-C chemokine receptor activity Source: UniProtKB
- G protein-coupled receptor activity Source: UniProtKB
GO - Biological processi
- chemokine-mediated signaling pathway Source: MGI
- cytoskeleton organization Source: Ensembl
- G protein-coupled receptor signaling pathway Source: UniProtKB
- negative regulation of neuronal action potential Source: Ensembl
- negative regulation of voltage-gated calcium channel activity Source: UniProtKB
- positive regulation of cytosolic calcium ion concentration Source: UniProtKB
- positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway Source: GO_Central
- positive regulation of Rho protein signal transduction Source: GO_Central
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
PathwayCommonsi | Q9HC97 |
Reactomei | R-HSA-373076, Class A/1 (Rhodopsin-like receptors) |
SIGNORi | Q9HC97 |
Protein family/group databases
TCDBi | 9.A.14.13.8, the g-protein-coupled receptor (gpcr) family |
Names & Taxonomyi
Protein namesi | Recommended name: G-protein coupled receptor 35Alternative name(s): Kynurenic acid receptor Short name: KYNA receptor |
Gene namesi | Name:GPR35 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:4492, GPR35 |
MIMi | 602646, gene |
neXtProti | NX_Q9HC97 |
VEuPathDBi | HostDB:ENSG00000178623.11 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Internalized to the cytoplasm after exposure to kynurenic acid.
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: Reactome
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 24 | ExtracellularSequence analysisAdd BLAST | 24 | |
Transmembranei | 25 – 45 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 46 – 56 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 57 – 77 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 78 – 90 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 91 – 112 | Helical; Name=3Sequence analysisAdd BLAST | 22 | |
Topological domaini | 113 – 135 | CytoplasmicSequence analysisAdd BLAST | 23 | |
Transmembranei | 136 – 156 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 157 – 174 | ExtracellularSequence analysisAdd BLAST | 18 | |
Transmembranei | 175 – 195 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 196 – 218 | CytoplasmicSequence analysisAdd BLAST | 23 | |
Transmembranei | 219 – 239 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 240 – 258 | ExtracellularSequence analysisAdd BLAST | 19 | |
Transmembranei | 259 – 279 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 280 – 309 | CytoplasmicSequence analysisAdd BLAST | 30 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 2859 |
MalaCardsi | GPR35 |
OpenTargetsi | ENSG00000178623 |
Orphaneti | 171, Primary sclerosing cholangitis |
PharmGKBi | PA28880 |
Miscellaneous databases
Pharosi | Q9HC97, Tchem |
Chemistry databases
ChEMBLi | CHEMBL1293267 |
DrugBanki | DB00695, Furosemide DB11937, Kynurenic Acid DB11156, Pyrantel |
DrugCentrali | Q9HC97 |
GuidetoPHARMACOLOGYi | 102 |
Genetic variation databases
BioMutai | GPR35 |
DMDMi | 68068087 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069563 | 1 – 309 | G-protein coupled receptor 35Add BLAST | 309 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 2 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 89 ↔ 162 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
jPOSTi | Q9HC97 |
MassIVEi | Q9HC97 |
PaxDbi | Q9HC97 |
PeptideAtlasi | Q9HC97 |
PRIDEi | Q9HC97 |
ProteomicsDBi | 81658 [Q9HC97-1] |
PTM databases
GlyGeni | Q9HC97, 1 site |
iPTMneti | Q9HC97 |
PhosphoSitePlusi | Q9HC97 |
Expressioni
Tissue specificityi
Predominantly expressed in immune and gastrointestinal tissues.1 Publication
Gene expression databases
Bgeei | ENSG00000178623, Expressed in mucosa of transverse colon and 126 other tissues |
Genevisiblei | Q9HC97, HS |
Organism-specific databases
HPAi | ENSG00000178623, Tissue enriched (intestine) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 109117, 7 interactors |
IntActi | Q9HC97, 64 interactors |
MINTi | Q9HC97 |
STRINGi | 9606.ENSP00000411788 |
Chemistry databases
BindingDBi | Q9HC97 |
Miscellaneous databases
RNActi | Q9HC97, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S0QN, Eukaryota |
GeneTreei | ENSGT01010000222244 |
HOGENOMi | CLU_009579_8_2_1 |
InParanoidi | Q9HC97 |
OMAi | DANCCLD |
OrthoDBi | 966250at2759 |
PhylomeDBi | Q9HC97 |
TreeFami | TF335578 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q9HC97-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNGTYNTCGS SDLTWPPAIK LGFYAYLGVL LVLGLLLNSL ALWVFCCRMQ
60 70 80 90 100
QWTETRIYMT NLAVADLCLL CTLPFVLHSL RDTSDTPLCQ LSQGIYLTNR
110 120 130 140 150
YMSISLVTAI AVDRYVAVRH PLRARGLRSP RQAAAVCAVL WVLVIGSLVA
160 170 180 190 200
RWLLGIQEGG FCFRSTRHNF NSMAFPLLGF YLPLAVVVFC SLKVVTALAQ
210 220 230 240 250
RPPTDVGQAE ATRKAARMVW ANLLVFVVCF LPLHVGLTVR LAVGWNACAL
260 270 280 290 300
LETIRRALYI TSKLSDANCC LDAICYYYMA KEFQEASALA VAPSAKAHKS
QDSLCVTLA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Sequence conflicti | 174 | A → R in AAC52028 (PubMed:9479505).Curated | 1 | ||
Isoform 2 (identifier: Q9HC97-2) | |||||
Sequence conflicti | 13 | R → H in AK172786 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_013601 | 25 | A → T1 PublicationCorresponds to variant dbSNP:rs35146537Ensembl. | 1 | |
Natural variantiVAR_013602 | 29 | V → I1 PublicationCorresponds to variant dbSNP:rs139197368Ensembl. | 1 | |
Natural variantiVAR_033467 | 76 | V → M. Corresponds to variant dbSNP:rs13387859Ensembl. | 1 | |
Natural variantiVAR_013603 | 108 | T → M1 PublicationCorresponds to variant dbSNP:rs3749171Ensembl. | 1 | |
Natural variantiVAR_013604 | 125 | R → S1 PublicationCorresponds to variant dbSNP:rs34778053Ensembl. | 1 | |
Natural variantiVAR_013605 | 253 | T → M1 PublicationCorresponds to variant dbSNP:rs12468485Ensembl. | 1 | |
Natural variantiVAR_013606 | 294 | S → R4 PublicationsCorresponds to variant dbSNP:rs3749172Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_045871 | 1 | M → MLSGSRAVPTPHRGSEELLK YMLHSPCVSLTM in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF027957 Genomic DNA Translation: AAC52028.1 AF089087 mRNA Translation: AAG17965.1 AY275467 Genomic DNA Translation: AAP32299.1 AK172786 mRNA No translation available. CR541765 mRNA Translation: CAG46564.1 AC124862 Genomic DNA Translation: AAX88945.1 BC095500 mRNA Translation: AAH95500.1 BC117453 mRNA Translation: AAI17454.2 BC117455 mRNA Translation: AAI17456.2 |
CCDSi | CCDS2541.1 [Q9HC97-1] CCDS56174.1 [Q9HC97-2] |
RefSeqi | NP_001182310.1, NM_001195381.1 [Q9HC97-2] NP_001182311.1, NM_001195382.1 [Q9HC97-2] NP_005292.2, NM_005301.3 [Q9HC97-1] |
Genome annotation databases
Ensembli | ENST00000319838; ENSP00000322731; ENSG00000178623 [Q9HC97-1] ENST00000403859; ENSP00000385140; ENSG00000178623 [Q9HC97-1] ENST00000407714; ENSP00000384263; ENSG00000178623 [Q9HC97-1] ENST00000430267; ENSP00000411788; ENSG00000178623 [Q9HC97-2] ENST00000438013; ENSP00000415890; ENSG00000178623 [Q9HC97-1] |
GeneIDi | 2859 |
KEGGi | hsa:2859 |
UCSCi | uc002vzs.4, human [Q9HC97-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF027957 Genomic DNA Translation: AAC52028.1 AF089087 mRNA Translation: AAG17965.1 AY275467 Genomic DNA Translation: AAP32299.1 AK172786 mRNA No translation available. CR541765 mRNA Translation: CAG46564.1 AC124862 Genomic DNA Translation: AAX88945.1 BC095500 mRNA Translation: AAH95500.1 BC117453 mRNA Translation: AAI17454.2 BC117455 mRNA Translation: AAI17456.2 |
CCDSi | CCDS2541.1 [Q9HC97-1] CCDS56174.1 [Q9HC97-2] |
RefSeqi | NP_001182310.1, NM_001195381.1 [Q9HC97-2] NP_001182311.1, NM_001195382.1 [Q9HC97-2] NP_005292.2, NM_005301.3 [Q9HC97-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 109117, 7 interactors |
IntActi | Q9HC97, 64 interactors |
MINTi | Q9HC97 |
STRINGi | 9606.ENSP00000411788 |
Chemistry databases
BindingDBi | Q9HC97 |
ChEMBLi | CHEMBL1293267 |
DrugBanki | DB00695, Furosemide DB11937, Kynurenic Acid DB11156, Pyrantel |
DrugCentrali | Q9HC97 |
GuidetoPHARMACOLOGYi | 102 |
Protein family/group databases
TCDBi | 9.A.14.13.8, the g-protein-coupled receptor (gpcr) family |
GPCRDBi | Search... |
PTM databases
GlyGeni | Q9HC97, 1 site |
iPTMneti | Q9HC97 |
PhosphoSitePlusi | Q9HC97 |
Genetic variation databases
BioMutai | GPR35 |
DMDMi | 68068087 |
Proteomic databases
jPOSTi | Q9HC97 |
MassIVEi | Q9HC97 |
PaxDbi | Q9HC97 |
PeptideAtlasi | Q9HC97 |
PRIDEi | Q9HC97 |
ProteomicsDBi | 81658 [Q9HC97-1] |
Protocols and materials databases
Antibodypediai | 34526, 222 antibodies |
DNASUi | 2859 |
Genome annotation databases
Ensembli | ENST00000319838; ENSP00000322731; ENSG00000178623 [Q9HC97-1] ENST00000403859; ENSP00000385140; ENSG00000178623 [Q9HC97-1] ENST00000407714; ENSP00000384263; ENSG00000178623 [Q9HC97-1] ENST00000430267; ENSP00000411788; ENSG00000178623 [Q9HC97-2] ENST00000438013; ENSP00000415890; ENSG00000178623 [Q9HC97-1] |
GeneIDi | 2859 |
KEGGi | hsa:2859 |
UCSCi | uc002vzs.4, human [Q9HC97-1] |
Organism-specific databases
CTDi | 2859 |
DisGeNETi | 2859 |
GeneCardsi | GPR35 |
HGNCi | HGNC:4492, GPR35 |
HPAi | ENSG00000178623, Tissue enriched (intestine) |
MalaCardsi | GPR35 |
MIMi | 602646, gene |
neXtProti | NX_Q9HC97 |
OpenTargetsi | ENSG00000178623 |
Orphaneti | 171, Primary sclerosing cholangitis |
PharmGKBi | PA28880 |
VEuPathDBi | HostDB:ENSG00000178623.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502S0QN, Eukaryota |
GeneTreei | ENSGT01010000222244 |
HOGENOMi | CLU_009579_8_2_1 |
InParanoidi | Q9HC97 |
OMAi | DANCCLD |
OrthoDBi | 966250at2759 |
PhylomeDBi | Q9HC97 |
TreeFami | TF335578 |
Enzyme and pathway databases
PathwayCommonsi | Q9HC97 |
Reactomei | R-HSA-373076, Class A/1 (Rhodopsin-like receptors) |
SIGNORi | Q9HC97 |
Miscellaneous databases
BioGRID-ORCSi | 2859, 6 hits in 876 CRISPR screens |
ChiTaRSi | GPR35, human |
GenomeRNAii | 2859 |
Pharosi | Q9HC97, Tchem |
PROi | PR:Q9HC97 |
RNActi | Q9HC97, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000178623, Expressed in mucosa of transverse colon and 126 other tissues |
Genevisiblei | Q9HC97, HS |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GPR35_HUMAN | |
Accessioni | Q9HC97Primary (citable) accession number: Q9HC97 Secondary accession number(s): J3KR30 Q86UH4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 14, 2001 |
Last sequence update: | June 21, 2005 | |
Last modified: | February 10, 2021 | |
This is version 156 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families