UniProtKB - Q9HC62 (SENP2_HUMAN)
Sentrin-specific protease 2
SENP2
Functioni
Protease that catalyzes two essential functions in the SUMO pathway (PubMed:11896061, PubMed:12192048, PubMed:20194620, PubMed:21965678, PubMed:15296745).
The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins (PubMed:15296745).
The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein (PubMed:20194620, PubMed:21965678, PubMed:15296745).
May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway (By similarity).
Deconjugates SUMO2 from MTA1 (PubMed:21965678).
Plays a dynamic role in adipogenesis by desumoylating and promoting the stabilization of CEBPB (PubMed:20194620).
Acts as a regulator of the cGAS-STING pathway by catalyzing desumoylation of CGAS and STING1 during the late phase of viral infection (By similarity).
By similarity5 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 478 | 1 Publication | 1 | |
Active sitei | 495 | 1 Publication | 1 | |
Active sitei | 548 | Nucleophile1 Publication | 1 |
GO - Molecular functioni
- SUMO-specific endopeptidase activity Source: UniProtKB
- SUMO-specific isopeptidase activity Source: Ensembl
GO - Biological processi
- fat cell differentiation Source: UniProtKB
- heart development Source: Ensembl
- labyrinthine layer development Source: Ensembl
- mRNA transport Source: UniProtKB-KW
- negative regulation of chromatin binding Source: Ensembl
- negative regulation of protein ubiquitination Source: CACAO
- positive regulation of protein ubiquitination Source: CACAO
- positive regulation of transcription by RNA polymerase II Source: Ensembl
- protein destabilization Source: CACAO
- protein desumoylation Source: UniProtKB
- protein transport Source: UniProtKB-KW
- regulation of DNA endoreduplication Source: Ensembl
- regulation of G1/S transition of mitotic cell cycle Source: Ensembl
- regulation of Wnt signaling pathway Source: UniProtKB
- spongiotrophoblast layer development Source: Ensembl
- trophoblast giant cell differentiation Source: Ensembl
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | mRNA transport, Protein transport, Translocation, Transport, Ubl conjugation pathway, Wnt signaling pathway |
Enzyme and pathway databases
BRENDAi | 3.4.22.B71, 2681 |
PathwayCommonsi | Q9HC62 |
Reactomei | R-HSA-3065679, SUMO is proteolytically processed |
SignaLinki | Q9HC62 |
Protein family/group databases
MEROPSi | C48.007 |
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Sentrin-specific protease 2Curated (EC:3.4.22.-2 Publications)Alternative name(s): Axam2By similarity SMT3-specific isopeptidase 2By similarity Short name: Smt3ip2By similarity Sentrin/SUMO-specific protease SENP21 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:23116, SENP2 |
MIMi | 608261, gene |
neXtProti | NX_Q9HC62 |
VEuPathDBi | HostDB:ENSG00000163904 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Nucleus
- nuclear pore complex 1 Publication
- Nucleus membrane 1 Publication; Peripheral membrane protein 1 Publication; Nucleoplasmic side 1 Publication
Note: Shuttles between cytoplasm and nucleus.1 Publication
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear membrane Source: UniProtKB-SubCell
- nuclear pore Source: UniProtKB
- nucleoplasm Source: Reactome
- nucleus Source: GO_Central
- PML body Source: Ensembl
Keywords - Cellular componenti
Cytoplasm, Membrane, Nuclear pore complex, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 548 | C → S: Does not desumoylate CEBPB. 1 Publication | 1 | |
Mutagenesisi | 576 – 577 | RK → LM: Does not desumoylate CEBPB. 1 Publication | 2 |
Organism-specific databases
DisGeNETi | 59343 |
OpenTargetsi | ENSG00000163904 |
PharmGKBi | PA134955185 |
Miscellaneous databases
Pharosi | Q9HC62, Tbio |
Chemistry databases
ChEMBLi | CHEMBL2176776 |
Genetic variation databases
BioMutai | SENP2 |
DMDMi | 143811458 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000101718 | 1 – 589 | Sentrin-specific protease 2Add BLAST | 589 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 32 | PhosphoserineCombined sources | 1 | |
Modified residuei | 333 | PhosphoserineCombined sources | 1 | |
Modified residuei | 344 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9HC62 |
jPOSTi | Q9HC62 |
MassIVEi | Q9HC62 |
MaxQBi | Q9HC62 |
PaxDbi | Q9HC62 |
PeptideAtlasi | Q9HC62 |
PRIDEi | Q9HC62 |
ProteomicsDBi | 4840 81643 [Q9HC62-1] |
PTM databases
iPTMneti | Q9HC62 |
PhosphoSitePlusi | Q9HC62 |
Expressioni
Gene expression databases
Bgeei | ENSG00000163904, Expressed in testis and 225 other tissues |
ExpressionAtlasi | Q9HC62, baseline and differential |
Genevisiblei | Q9HC62, HS |
Organism-specific databases
HPAi | ENSG00000163904, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Q9HC62
Protein-protein interaction databases
BioGRIDi | 121885, 155 interactors |
DIPi | DIP-29254N |
IntActi | Q9HC62, 42 interactors |
MINTi | Q9HC62 |
STRINGi | 9606.ENSP00000296257 |
Chemistry databases
BindingDBi | Q9HC62 |
Miscellaneous databases
RNActi | Q9HC62, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9HC62 |
SMRi | Q9HC62 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9HC62 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 155 – 176 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 396 – 560 | Protease1 PublicationAdd BLAST | 165 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 28 – 31 | Nuclear localization signal1 Publication | 4 | |
Motifi | 46 – 51 | Nuclear localization signal1 Publication | 6 | |
Motifi | 317 – 332 | Nuclear export signal1 PublicationAdd BLAST | 16 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0778, Eukaryota |
GeneTreei | ENSGT00940000154951 |
HOGENOMi | CLU_024324_4_0_1 |
InParanoidi | Q9HC62 |
OMAi | PGMYRWL |
OrthoDBi | 1480705at2759 |
PhylomeDBi | Q9HC62 |
TreeFami | TF316289 |
Family and domain databases
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR003653, Peptidase_C48_C |
Pfami | View protein in Pfam PF02902, Peptidase_C48, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS50600, ULP_PROTEASE, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MYRWLVRILG TIFRFCDRSV PPARALLKRR RSDSTLFSTV DTDEIPAKRP
60 70 80 90 100
RLDCFIHQVK NSLYNAASLF GFPFQLTTKP MVTSACNGTR NVAPSGEVFS
110 120 130 140 150
NSSSCELTGS GSWNNMLKLG NKSPNGISDY PKIRVTVTRD QPRRVLPSFG
160 170 180 190 200
FTLNSEGCNR RPGGRRHSKG NPESSLMWKP QEQAVTEMIS EESGKGLRRP
210 220 230 240 250
HCTVEEGVQK EEREKYRKLL ERLKESGHGN SVCPVTSNYH SSQRSQMDTL
260 270 280 290 300
KTKGWGEEQN HGVKTTQFVP KQYRLVETRG PLCSLRSEKR CSKGKITDTE
310 320 330 340 350
TMVGIRFENE SRRGYQLEPD LSEEVSARLR LGSGSNGLLR RKVSIIETKE
360 370 380 390 400
KNCSGKERDR RTDDLLELTE DMEKEISNAL GHGPQDEILS SAFKLRITRG
410 420 430 440 450
DIQTLKNYHW LNDEVINFYM NLLVERNKKQ GYPALHVFST FFYPKLKSGG
460 470 480 490 500
YQAVKRWTKG VNLFEQEIIL VPIHRKVHWS LVVIDLRKKC LKYLDSMGQK
510 520 530 540 550
GHRICEILLQ YLQDESKTKR NSDLNLLEWT HHSMKPHEIP QQLNGSDCGM
560 570 580
FTCKYADYIS RDKPITFTQH QMPLFRKKMV WEILHQQLL
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH7C1A0 | H7C1A0_HUMAN | Sentrin-specific protease 2 | SENP2 | 203 | Annotation score: | ||
C9IYB7 | C9IYB7_HUMAN | Sentrin-specific protease 2 | SENP2 | 273 | Annotation score: | ||
F8WCX8 | F8WCX8_HUMAN | Sentrin-specific protease 2 | SENP2 | 60 | Annotation score: | ||
F8WED6 | F8WED6_HUMAN | Sentrin-specific protease 2 | SENP2 | 54 | Annotation score: | ||
F8WET6 | F8WET6_HUMAN | Sentrin-specific protease 2 | SENP2 | 105 | Annotation score: | ||
F8WED7 | F8WED7_HUMAN | Sentrin-specific protease 2 | SENP2 | 104 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 39 | T → TA in AAG15309 (Ref. 1) Curated | 1 | |
Sequence conflicti | 403 | Q → H in BAB55222 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_029650 | 301 | T → K4 PublicationsCorresponds to variant dbSNP:rs6762208Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056697 | 1 – 33 | MYRWL…RRRSD → MDSHPCLYCGPQDSEGTNWQ TSP in isoform 2. 1 PublicationAdd BLAST | 33 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF151697 mRNA Translation: AAG15309.2 AB037752 mRNA Translation: BAA92569.2 AK027599 mRNA Translation: BAB55222.1 AK298628 mRNA Translation: BAG60804.1 AC016961 Genomic DNA No translation available. AC133473 Genomic DNA No translation available. BC040609 mRNA Translation: AAH40609.1 AL834380 mRNA Translation: CAD39043.1 |
CCDSi | CCDS33902.1 [Q9HC62-1] |
RefSeqi | NP_067640.2, NM_021627.2 [Q9HC62-1] |
Genome annotation databases
Ensembli | ENST00000296257.10; ENSP00000296257.5; ENSG00000163904.13 |
GeneIDi | 59343 |
KEGGi | hsa:59343 |
MANE-Selecti | ENST00000296257.10; ENSP00000296257.5; NM_021627.3; NP_067640.2 |
UCSCi | uc003fpn.4, human [Q9HC62-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF151697 mRNA Translation: AAG15309.2 AB037752 mRNA Translation: BAA92569.2 AK027599 mRNA Translation: BAB55222.1 AK298628 mRNA Translation: BAG60804.1 AC016961 Genomic DNA No translation available. AC133473 Genomic DNA No translation available. BC040609 mRNA Translation: AAH40609.1 AL834380 mRNA Translation: CAD39043.1 |
CCDSi | CCDS33902.1 [Q9HC62-1] |
RefSeqi | NP_067640.2, NM_021627.2 [Q9HC62-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1TGZ | X-ray | 2.80 | A | 364-589 | [»] | |
1TH0 | X-ray | 2.20 | A/B | 364-589 | [»] | |
2IO0 | X-ray | 2.30 | A | 364-589 | [»] | |
2IO1 | X-ray | 2.60 | A/C/E | 364-589 | [»] | |
2IO2 | X-ray | 2.90 | A | 364-589 | [»] | |
2IO3 | X-ray | 3.20 | A | 364-589 | [»] | |
3ZO5 | X-ray | 2.15 | A | 363-589 | [»] | |
5AEK | X-ray | 3.00 | A/C/E/G/I/K/M/O/Q/S/U/W | 366-589 | [»] | |
AlphaFoldDBi | Q9HC62 | |||||
SMRi | Q9HC62 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 121885, 155 interactors |
DIPi | DIP-29254N |
IntActi | Q9HC62, 42 interactors |
MINTi | Q9HC62 |
STRINGi | 9606.ENSP00000296257 |
Chemistry databases
BindingDBi | Q9HC62 |
ChEMBLi | CHEMBL2176776 |
Protein family/group databases
MEROPSi | C48.007 |
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
PTM databases
iPTMneti | Q9HC62 |
PhosphoSitePlusi | Q9HC62 |
Genetic variation databases
BioMutai | SENP2 |
DMDMi | 143811458 |
Proteomic databases
EPDi | Q9HC62 |
jPOSTi | Q9HC62 |
MassIVEi | Q9HC62 |
MaxQBi | Q9HC62 |
PaxDbi | Q9HC62 |
PeptideAtlasi | Q9HC62 |
PRIDEi | Q9HC62 |
ProteomicsDBi | 4840 81643 [Q9HC62-1] |
Protocols and materials databases
Antibodypediai | 33840, 728 antibodies from 35 providers |
DNASUi | 59343 |
Genome annotation databases
Ensembli | ENST00000296257.10; ENSP00000296257.5; ENSG00000163904.13 |
GeneIDi | 59343 |
KEGGi | hsa:59343 |
MANE-Selecti | ENST00000296257.10; ENSP00000296257.5; NM_021627.3; NP_067640.2 |
UCSCi | uc003fpn.4, human [Q9HC62-1] |
Organism-specific databases
CTDi | 59343 |
DisGeNETi | 59343 |
GeneCardsi | SENP2 |
HGNCi | HGNC:23116, SENP2 |
HPAi | ENSG00000163904, Low tissue specificity |
MIMi | 608261, gene |
neXtProti | NX_Q9HC62 |
OpenTargetsi | ENSG00000163904 |
PharmGKBi | PA134955185 |
VEuPathDBi | HostDB:ENSG00000163904 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0778, Eukaryota |
GeneTreei | ENSGT00940000154951 |
HOGENOMi | CLU_024324_4_0_1 |
InParanoidi | Q9HC62 |
OMAi | PGMYRWL |
OrthoDBi | 1480705at2759 |
PhylomeDBi | Q9HC62 |
TreeFami | TF316289 |
Enzyme and pathway databases
BRENDAi | 3.4.22.B71, 2681 |
PathwayCommonsi | Q9HC62 |
Reactomei | R-HSA-3065679, SUMO is proteolytically processed |
SignaLinki | Q9HC62 |
Miscellaneous databases
BioGRID-ORCSi | 59343, 20 hits in 1082 CRISPR screens |
ChiTaRSi | SENP2, human |
EvolutionaryTracei | Q9HC62 |
GeneWikii | SENP2 |
GenomeRNAii | 59343 |
Pharosi | Q9HC62, Tbio |
PROi | PR:Q9HC62 |
RNActi | Q9HC62, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000163904, Expressed in testis and 225 other tissues |
ExpressionAtlasi | Q9HC62, baseline and differential |
Genevisiblei | Q9HC62, HS |
Family and domain databases
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR003653, Peptidase_C48_C |
Pfami | View protein in Pfam PF02902, Peptidase_C48, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS50600, ULP_PROTEASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SENP2_HUMAN | |
Accessioni | Q9HC62Primary (citable) accession number: Q9HC62 Secondary accession number(s): B4DQ42 Q9P2L5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2002 |
Last sequence update: | April 3, 2007 | |
Last modified: | May 25, 2022 | |
This is version 187 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families