UniProtKB - Q9HBW1 (LRRC4_HUMAN)
Protein
Leucine-rich repeat-containing protein 4
Gene
LRRC4
Organism
Homo sapiens (Human)
Status
Functioni
Synaptic adhesion protein. Regulates the formation of exitatory synapses through the recruitment of pre-and-postsynaptic proteins. Organize the lamina/pathway-specific differentiation of dendrites. Plays an important role for auditory synaptic responses. Involved in the suppression of glioma (By similarity).By similarity
GO - Biological processi
- excitatory synapse assembly Source: Ensembl
- modulation of chemical synaptic transmission Source: Ensembl
- postsynaptic density protein 95 clustering Source: Ensembl
- synaptic membrane adhesion Source: Ensembl
Enzyme and pathway databases
PathwayCommonsi | Q9HBW1 |
Names & Taxonomyi
Protein namesi | Recommended name: Leucine-rich repeat-containing protein 4Alternative name(s): Brain tumor-associated protein BAG Nasopharyngeal carcinoma-associated gene 14 protein Netrin-G2 ligand Short name: NGL-2 |
Gene namesi | Name:LRRC4 Synonyms:BAG ORF Names:NAG14, UNQ554/PRO1111 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000128594.7 |
HGNCi | HGNC:15586, LRRC4 |
MIMi | 610486, gene |
neXtProti | NX_Q9HBW1 |
Subcellular locationi
Plasma membrane
- postsynaptic cell membrane By similarity
Other locations
Note: LRRC4 and DLG4 are interdependent for synaptic localization.By similarity
Plasma Membrane
- integral component of postsynaptic density membrane Source: Ensembl
Other locations
- dendritic spine Source: Ensembl
- excitatory synapse Source: Ensembl
- glutamatergic synapse Source: Ensembl
- Schaffer collateral - CA1 synapse Source: Ensembl
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 39 – 527 | ExtracellularSequence analysisAdd BLAST | 489 | |
Transmembranei | 528 – 548 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 549 – 653 | CytoplasmicSequence analysisAdd BLAST | 105 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, SynapsePathology & Biotechi
Organism-specific databases
DisGeNETi | 64101 |
OpenTargetsi | ENSG00000128594 |
PharmGKBi | PA30463 |
Miscellaneous databases
Pharosi | Q9HBW1, Tbio |
Polymorphism and mutation databases
BioMutai | LRRC4 |
DMDMi | 51701696 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 38 | Sequence analysisAdd BLAST | 38 | |
ChainiPRO_0000014833 | 39 – 653 | Leucine-rich repeat-containing protein 4Add BLAST | 615 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 46 ↔ 52 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 50 ↔ 61 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 277 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 304 ↔ 329 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 306 ↔ 350 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 322 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 363 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 374 ↔ 424 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 388 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 410 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 434 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 440 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 447 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 450 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
N-glycosylated.By similarity
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MassIVEi | Q9HBW1 |
PaxDbi | Q9HBW1 |
PeptideAtlasi | Q9HBW1 |
PRIDEi | Q9HBW1 |
ProteomicsDBi | 81601 |
PTM databases
GlyConnecti | 1453, 1 N-Linked glycan (1 site) |
GlyGeni | Q9HBW1, 9 sites |
iPTMneti | Q9HBW1 |
PhosphoSitePlusi | Q9HBW1 |
Expressioni
Tissue specificityi
Specifically expressed in brain.1 Publication
Gene expression databases
Bgeei | ENSG00000128594, Expressed in cerebellum and 164 other tissues |
ExpressionAtlasi | Q9HBW1, baseline and differential |
Genevisiblei | Q9HBW1, HS |
Organism-specific databases
HPAi | ENSG00000128594, Tissue enhanced (brain) |
Interactioni
Subunit structurei
Interacts with DLG4 (By similarity).
Interacts (via LRR repeats) with NTNG2.
Forms a complex with DLG4 and with NMDA receptors.
By similarity1 PublicationBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 122061, 15 interactors |
IntActi | Q9HBW1, 13 interactors |
MINTi | Q9HBW1 |
STRINGi | 9606.ENSP00000249363 |
Miscellaneous databases
RNActi | Q9HBW1, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9HBW1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9HBW1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 39 – 75 | LRRNTAdd BLAST | 37 | |
Repeati | 76 – 97 | LRR 1Add BLAST | 22 | |
Repeati | 100 – 121 | LRR 2Add BLAST | 22 | |
Repeati | 124 – 145 | LRR 3Add BLAST | 22 | |
Repeati | 148 – 169 | LRR 4Add BLAST | 22 | |
Repeati | 172 – 194 | LRR 5Add BLAST | 23 | |
Repeati | 197 – 218 | LRR 6Add BLAST | 22 | |
Repeati | 219 – 240 | LRR 7Add BLAST | 22 | |
Repeati | 243 – 264 | LRR 8Add BLAST | 22 | |
Repeati | 267 – 288 | LRR 9Add BLAST | 22 | |
Domaini | 300 – 352 | LRRCTAdd BLAST | 53 | |
Domaini | 353 – 442 | Ig-likeAdd BLAST | 90 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 455 – 526 | Thr-richAdd BLAST | 72 |
Domaini
The last 4 C-terminal residues bind to the first 2 PDZ domains of DLG4.By similarity
Keywords - Domaini
Immunoglobulin domain, Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0619, Eukaryota |
GeneTreei | ENSGT00940000159260 |
HOGENOMi | CLU_000288_18_24_1 |
InParanoidi | Q9HBW1 |
OMAi | GPTTEMP |
OrthoDBi | 282791at2759 |
PhylomeDBi | Q9HBW1 |
TreeFami | TF324303 |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR000483, Cys-rich_flank_reg_C IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf IPR026882, Lrrc4 IPR000372, LRRNT |
PANTHERi | PTHR24369:SF9, PTHR24369:SF9, 1 hit |
Pfami | View protein in Pfam PF07679, I-set, 1 hit PF13855, LRR_8, 3 hits |
SMARTi | View protein in SMART SM00409, IG, 1 hit SM00408, IGc2, 1 hit SM00369, LRR_TYP, 7 hits SM00082, LRRCT, 1 hit SM00013, LRRNT, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit PS51450, LRR, 7 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q9HBW1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLLWQVTVH HHTWNAILLP FVYLTAQVWI LCAAIAAAAS AGPQNCPSVC
60 70 80 90 100
SCSNQFSKVV CTRRGLSEVP QGIPSNTRYL NLMENNIQMI QADTFRHLHH
110 120 130 140 150
LEVLQLGRNS IRQIEVGAFN GLASLNTLEL FDNWLTVIPS GAFEYLSKLR
160 170 180 190 200
ELWLRNNPIE SIPSYAFNRV PSLMRLDLGE LKKLEYISEG AFEGLFNLKY
210 220 230 240 250
LNLGMCNIKD MPNLTPLVGL EELEMSGNHF PEIRPGSFHG LSSLKKLWVM
260 270 280 290 300
NSQVSLIERN AFDGLASLVE LNLAHNNLSS LPHDLFTPLR YLVELHLHHN
310 320 330 340 350
PWNCDCDILW LAWWLREYIP TNSTCCGRCH APMHMRGRYL VEVDQASFQC
360 370 380 390 400
SAPFIMDAPR DLNISEGRMA ELKCRTPPMS SVKWLLPNGT VLSHASRHPR
410 420 430 440 450
ISVLNDGTLN FSHVLLSDTG VYTCMVTNVA GNSNASAYLN VSTAELNTSN
460 470 480 490 500
YSFFTTVTVE TTEISPEDTT RKYKPVPTTS TGYQPAYTTS TTVLIQTTRV
510 520 530 540 550
PKQVAVPATD TTDKMQTSLD EVMKTTKIII GCFVAVTLLA AAMLIVFYKL
560 570 580 590 600
RKRHQQRSTV TAARTVEIIQ VDEDIPAATS AAATAAPSGV SGEGAVVLPT
610 620 630 640 650
IHDHINYNTY KPAHGAHWTE NSLGNSLHPT VTTISEPYII QTHTKDKVQE
TQI
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JA92 | C9JA92_HUMAN | Leucine-rich repeat-containing prot... | LRRC4 | 94 | Annotation score: | ||
C9J7K7 | C9J7K7_HUMAN | Leucine-rich repeat-containing prot... | LRRC4 | 50 | Annotation score: | ||
C9J7V8 | C9J7V8_HUMAN | Leucine-rich repeat-containing prot... | LRRC4 | 78 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 4 | L → S in BAD18737 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 253 – 257 | QVSLI → H in CAC82651 (Ref. 2) Curated | 5 | |
Sequence conflicti | 300 | N → D in BAD18737 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 315 | L → F in BAD18737 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_035519 | 579 | T → A in a colorectal cancer sample; somatic mutation. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF196976 mRNA Translation: AAG28019.2 AJ297858 mRNA Translation: CAC82651.1 AY358307 mRNA Translation: AAQ88674.1 AK172751 mRNA Translation: BAD18737.1 AK314047 mRNA Translation: BAG36756.1 CH236947 Genomic DNA Translation: EAL24316.1 BC111561 mRNA Translation: AAI11562.1 BC111745 mRNA Translation: AAI11746.1 |
CCDSi | CCDS5799.1 |
RefSeqi | NP_071426.1, NM_022143.4 XP_011514763.1, XM_011516461.2 XP_016867994.1, XM_017012505.1 |
Genome annotation databases
Ensembli | ENST00000249363; ENSP00000249363; ENSG00000128594 |
GeneIDi | 64101 |
KEGGi | hsa:64101 |
UCSCi | uc003vmk.4, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF196976 mRNA Translation: AAG28019.2 AJ297858 mRNA Translation: CAC82651.1 AY358307 mRNA Translation: AAQ88674.1 AK172751 mRNA Translation: BAD18737.1 AK314047 mRNA Translation: BAG36756.1 CH236947 Genomic DNA Translation: EAL24316.1 BC111561 mRNA Translation: AAI11562.1 BC111745 mRNA Translation: AAI11746.1 |
CCDSi | CCDS5799.1 |
RefSeqi | NP_071426.1, NM_022143.4 XP_011514763.1, XM_011516461.2 XP_016867994.1, XM_017012505.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2DL9 | NMR | - | A | 353-442 | [»] | |
3ZYI | X-ray | 2.60 | A | 1-444 | [»] | |
SMRi | Q9HBW1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 122061, 15 interactors |
IntActi | Q9HBW1, 13 interactors |
MINTi | Q9HBW1 |
STRINGi | 9606.ENSP00000249363 |
PTM databases
GlyConnecti | 1453, 1 N-Linked glycan (1 site) |
GlyGeni | Q9HBW1, 9 sites |
iPTMneti | Q9HBW1 |
PhosphoSitePlusi | Q9HBW1 |
Polymorphism and mutation databases
BioMutai | LRRC4 |
DMDMi | 51701696 |
Proteomic databases
MassIVEi | Q9HBW1 |
PaxDbi | Q9HBW1 |
PeptideAtlasi | Q9HBW1 |
PRIDEi | Q9HBW1 |
ProteomicsDBi | 81601 |
Protocols and materials databases
Antibodypediai | 55653, 102 antibodies |
Genome annotation databases
Ensembli | ENST00000249363; ENSP00000249363; ENSG00000128594 |
GeneIDi | 64101 |
KEGGi | hsa:64101 |
UCSCi | uc003vmk.4, human |
Organism-specific databases
CTDi | 64101 |
DisGeNETi | 64101 |
EuPathDBi | HostDB:ENSG00000128594.7 |
GeneCardsi | LRRC4 |
HGNCi | HGNC:15586, LRRC4 |
HPAi | ENSG00000128594, Tissue enhanced (brain) |
MIMi | 610486, gene |
neXtProti | NX_Q9HBW1 |
OpenTargetsi | ENSG00000128594 |
PharmGKBi | PA30463 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0619, Eukaryota |
GeneTreei | ENSGT00940000159260 |
HOGENOMi | CLU_000288_18_24_1 |
InParanoidi | Q9HBW1 |
OMAi | GPTTEMP |
OrthoDBi | 282791at2759 |
PhylomeDBi | Q9HBW1 |
TreeFami | TF324303 |
Enzyme and pathway databases
PathwayCommonsi | Q9HBW1 |
Miscellaneous databases
BioGRID-ORCSi | 64101, 4 hits in 838 CRISPR screens |
EvolutionaryTracei | Q9HBW1 |
GeneWikii | LRRC4 |
GenomeRNAii | 64101 |
Pharosi | Q9HBW1, Tbio |
PROi | PR:Q9HBW1 |
RNActi | Q9HBW1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000128594, Expressed in cerebellum and 164 other tissues |
ExpressionAtlasi | Q9HBW1, baseline and differential |
Genevisiblei | Q9HBW1, HS |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR000483, Cys-rich_flank_reg_C IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf IPR026882, Lrrc4 IPR000372, LRRNT |
PANTHERi | PTHR24369:SF9, PTHR24369:SF9, 1 hit |
Pfami | View protein in Pfam PF07679, I-set, 1 hit PF13855, LRR_8, 3 hits |
SMARTi | View protein in SMART SM00409, IG, 1 hit SM00408, IGc2, 1 hit SM00369, LRR_TYP, 7 hits SM00082, LRRCT, 1 hit SM00013, LRRNT, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit PS51450, LRR, 7 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LRRC4_HUMAN | |
Accessioni | Q9HBW1Primary (citable) accession number: Q9HBW1 Secondary accession number(s): A4D0Y9 Q96A85 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 31, 2004 |
Last sequence update: | October 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 174 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations