Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Kinetochore protein Spc25

Gene

SPC25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:14699129, PubMed:14738735). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:14738735, PubMed:14699129). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).3 Publications

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: UniProtKB
  • mitotic spindle organization Source: UniProtKB

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein Spc25
Short name:
hSpc25
Gene namesi
Name:SPC25
Synonyms:SPBC25
ORF Names:AD024
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000152253.8
HGNCiHGNC:24031 SPC25
MIMi609395 gene
neXtProtiNX_Q9HBM1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000152253
PharmGKBiPA162404413

Polymorphism and mutation databases

BioMutaiSPC25
DMDMi74734256

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002495651 – 224Kinetochore protein Spc25Add BLAST224

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9HBM1
MaxQBiQ9HBM1
PaxDbiQ9HBM1
PeptideAtlasiQ9HBM1
PRIDEiQ9HBM1
ProteomicsDBi81577

PTM databases

CarbonylDBiQ9HBM1
iPTMnetiQ9HBM1
PhosphoSitePlusiQ9HBM1

Expressioni

Gene expression databases

BgeeiENSG00000152253
CleanExiHS_SPC25
ExpressionAtlasiQ9HBM1 baseline and differential
GenevisibleiQ9HBM1 HS

Organism-specific databases

HPAiHPA047144
HPA050544

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi121507, 44 interactors
ComplexPortaliCPX-550 Ndc80 complex
CORUMiQ9HBM1
DIPiDIP-35753N
IntActiQ9HBM1, 20 interactors
MINTiQ9HBM1
STRINGi9606.ENSP00000282074

Structurei

Secondary structure

1224
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi118 – 141Combined sources24
Beta strandi144 – 147Combined sources4
Beta strandi154 – 158Combined sources5
Beta strandi165 – 168Combined sources4
Beta strandi170 – 175Combined sources6
Beta strandi177 – 180Combined sources4
Helixi195 – 203Combined sources9
Helixi207 – 221Combined sources15

3D structure databases

ProteinModelPortaliQ9HBM1
SMRiQ9HBM1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HBM1

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 144Interaction with the N-terminus of SPBC24Add BLAST144
Regioni1 – 56Interaction with the NDC80-NUF2 subcomplexAdd BLAST56
Regioni145 – 224Interaction with the C-terminus of SPBC24Add BLAST80

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili61 – 142Sequence analysisAdd BLAST82

Sequence similaritiesi

Belongs to the SPC25 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4657 Eukaryota
ENOG4111Y5R LUCA
GeneTreeiENSGT00390000002220
HOGENOMiHOG000231978
HOVERGENiHBG080509
InParanoidiQ9HBM1
KOiK11550
OMAiDTSCQMV
OrthoDBiEOG091G14DQ
PhylomeDBiQ9HBM1
TreeFamiTF332941

Family and domain databases

InterProiView protein in InterPro
IPR013255 Spc25
PfamiView protein in Pfam
PF08234 Spindle_Spc25, 1 hit

Sequencei

Sequence statusi: Complete.

Q9HBM1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEDELALFD KSINEFWNKF KSTDTSCQMA GLRDTYKDSI KAFAEKLSVK
60 70 80 90 100
LKEEERMVEM FLEYQNQISR QNKLIQEKKD NLLKLIAEVK GKKQELEVLT
110 120 130 140 150
ANIQDLKEEY SRKKETISTA NKANAERLKR LQKSADLYKD RLGLEIRKIY
160 170 180 190 200
GEKLQFIFTN IDPKNPESPF MFSLHLNEAR DYEVSDSAPH LEGLAEFQEN
210 220
VRKTNNFSAF LANVRKAFTA TVYN
Length:224
Mass (Da):26,153
Last modified:March 1, 2001 - v1
Checksum:i6DED3216794F4512
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY456388 mRNA Translation: AAR88652.1
AF225416 mRNA Translation: AAG09718.1
CR457197 mRNA Translation: CAG33478.1
AK291093 mRNA Translation: BAF83782.1
AC069137 Genomic DNA Translation: AAY24098.1
CH471058 Genomic DNA Translation: EAX11289.1
CH471058 Genomic DNA Translation: EAX11290.1
BC022255 mRNA Translation: AAH22255.1
CCDSiCCDS2229.1
RefSeqiNP_065726.1, NM_020675.3
UniGeneiHs.421956

Genome annotation databases

EnsembliENST00000282074; ENSP00000282074; ENSG00000152253
ENST00000611144; ENSP00000481354; ENSG00000277909
ENST00000616584; ENSP00000483050; ENSG00000277909
GeneIDi57405
KEGGihsa:57405
UCSCiuc002uel.4 human

Similar proteinsi

Entry informationi

Entry nameiSPC25_HUMAN
AccessioniPrimary (citable) accession number: Q9HBM1
Secondary accession number(s): A8K4X8, D3DPC0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: March 1, 2001
Last modified: July 18, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health