UniProtKB - Q9HB55 (CP343_HUMAN)
Protein
Cytochrome P450 3A43
Gene
CYP3A43
Organism
Homo sapiens (Human)
Status
Functioni
Exhibits low testosterone 6-beta-hydroxylase activity.
Miscellaneous
Chimeric transcripts, characterized by CYP3A43 exon 1 joined at canonical splice sites to distinct sets of CYP3A4 or CYP3A5 exons, have been detected. All are possibly produced by trans-splicing. CYP3A43-CYP3A4 chimeric transcripts exist in 3 different combinations: CYP3A43 exon 1 joined in frame to CYP3A4 exons 2-13, CYP3A43 exon 1 joined in frame to CYP3A4 exons 4-13 and CYP3A43 exon 1 joined in frame to CYP3A4 exon 7-13. The longest chimeric isoform (CYP3A43 exon 1 joined to CYP3A4 exons 2-13) exhibits 6-beta-hydroxylase activity, while a shorter isoform (CYP3A43 exon 1 joined to CYP3A4 exons 4-13) does not. CYP3A43-CYP3A5 chimeric transcripts exist in 2 different combinations: CYP3A43 exon 1 joined in frame to CYP3A5 exon 11-13 and CYP3A43 exon 1 joined in frame to CYP3A5 exon 12-13. All chimeric transcripts are expressed at very low levels in the liver (PubMed:11726664).1 Publication
Catalytic activityi
- an organic molecule + O2 + reduced [NADPH—hemoprotein reductase] = an alcohol + H+ + H2O + oxidized [NADPH—hemoprotein reductase]EC:1.14.14.1
Cofactori
hemeBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 442 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- aromatase activity Source: UniProtKB-EC
- estrogen 16-alpha-hydroxylase activity Source: GO_Central
- heme binding Source: InterPro
- iron ion binding Source: InterPro
- monooxygenase activity Source: UniProtKB
- steroid hydroxylase activity Source: GO_Central
- testosterone 6-beta-hydroxylase activity Source: GO_Central
GO - Biological processi
- oxidative demethylation Source: GO_Central
- steroid metabolic process Source: GO_Central
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9HB55 |
Reactomei | R-HSA-211958, Miscellaneous substrates R-HSA-211981, Xenobiotics |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:CYP3A43 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000021461.16 |
HGNCi | HGNC:17450, CYP3A43 |
MIMi | 606534, gene |
neXtProti | NX_Q9HB55 |
Subcellular locationi
Endoplasmic reticulum
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: Reactome
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePathology & Biotechi
Organism-specific databases
DisGeNETi | 64816 |
OpenTargetsi | ENSG00000021461 |
PharmGKBi | PA427 |
Miscellaneous databases
Pharosi | Q9HB55, Tclin |
Chemistry databases
ChEMBLi | CHEMBL5792 |
DrugBanki | DB11703, Acalabrutinib DB13141, Ambroxol acefyllinate DB12267, Brigatinib DB01222, Budesonide DB09065, Cobicistat DB12483, Copanlisib DB08865, Crizotinib DB09102, Daclatasvir DB01234, Dexamethasone DB14649, Dexamethasone acetate DB11994, Diacerein DB08930, Dolutegravir DB11979, Elagolix DB11574, Elbasvir DB00593, Ethosuximide DB00176, Fluvoxamine DB12307, Foretinib DB13879, Glecaprevir DB09054, Idelalisib DB11757, Istradefylline DB11951, Lemborexant DB12070, Letermovir DB01601, Lopinavir DB12130, Lorlatinib DB09212, Loxoprofen DB00643, Mebendazole DB00959, Methylprednisolone DB06595, Midostaurin DB11792, Mirodenafil DB09205, Moxisylyte DB11605, Myrrh DB11691, Naldemedine DB01149, Nefazodone DB09048, Netupitant DB00334, Olanzapine DB09568, Omega-3-carboxylic acids DB06412, Oxymetholone DB00780, Phenelzine DB13878, Pibrentasvir DB01058, Praziquantel DB14631, Prednisolone phosphate DB00635, Prednisone DB13174, Rhein DB01045, Rifampicin DB01201, Rifapentine DB12332, Rucaparib DB15685, Selpercatinib DB12887, Tazemetostat DB00624, Testosterone DB13943, Testosterone cypionate DB13944, Testosterone enanthate DB13946, Testosterone undecanoate DB00599, Thiopental DB13179, Troleandomycin DB11652, Tucatinib DB15035, Zanubrutinib |
DrugCentrali | Q9HB55 |
Polymorphism and mutation databases
BioMutai | CYP3A43 |
DMDMi | 20137481 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051814 | 1 – 503 | Cytochrome P450 3A43Add BLAST | 503 |
Proteomic databases
jPOSTi | Q9HB55 |
MassIVEi | Q9HB55 |
PaxDbi | Q9HB55 |
PeptideAtlasi | Q9HB55 |
PRIDEi | Q9HB55 |
ProteomicsDBi | 61978 81485 [Q9HB55-1] 81486 [Q9HB55-2] 81487 [Q9HB55-3] 81488 [Q9HB55-4] |
PTM databases
iPTMneti | Q9HB55 |
PhosphoSitePlusi | Q9HB55 |
Expressioni
Tissue specificityi
Highest expression level in prostate. Also expressed in liver, kidney, pancreas, fetal liver and fetal skeletal muscle.2 Publications
Inductioni
By rifampicin.
Gene expression databases
Bgeei | ENSG00000021461, Expressed in body of pancreas and 111 other tissues |
ExpressionAtlasi | Q9HB55, baseline and differential |
Genevisiblei | Q9HB55, HS |
Organism-specific databases
HPAi | ENSG00000021461, Tissue enriched (liver) |
Interactioni
Protein-protein interaction databases
IntActi | Q9HB55, 1 interactor |
STRINGi | 9606.ENSP00000222382 |
Chemistry databases
BindingDBi | Q9HB55 |
Miscellaneous databases
RNActi | Q9HB55, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Phylogenomic databases
eggNOGi | KOG0158, Eukaryota |
GeneTreei | ENSGT00950000182958 |
HOGENOMi | CLU_001570_5_2_1 |
InParanoidi | Q9HB55 |
OMAi | CITWFGT |
OrthoDBi | 467733at2759 |
PhylomeDBi | Q9HB55 |
TreeFami | TF105087 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR008072, Cyt_P450_E_CYP3A IPR002402, Cyt_P450_E_grp-II IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00464, EP450II PR01689, EP450IICYP3A PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketNote: Additional isoforms seem to exist.
This entry has 5 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9HB55-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDLIPNFAME TWVLVATSLV LLYIYGTHSH KLFKKLGIPG PTPLPFLGTI
60 70 80 90 100
LFYLRGLWNF DRECNEKYGE MWGLYEGQQP MLVIMDPDMI KTVLVKECYS
110 120 130 140 150
VFTNQMPLGP MGFLKSALSF AEDEEWKRIR TLLSPAFTSV KFKEMVPIIS
160 170 180 190 200
QCGDMLVRSL RQEAENSKSI NLKDFFGAYT MDVITGTLFG VNLDSLNNPQ
210 220 230 240 250
DPFLKNMKKL LKLDFLDPFL LLISLFPFLT PVFEALNIGL FPKDVTHFLK
260 270 280 290 300
NSIERMKESR LKDKQKHRVD FFQQMIDSQN SKETKSHKAL SDLELVAQSI
310 320 330 340 350
IIIFAAYDTT STTLPFIMYE LATHPDVQQK LQEEIDAVLP NKAPVTYDAL
360 370 380 390 400
VQMEYLDMVV NETLRLFPVV SRVTRVCKKD IEINGVFIPK GLAVMVPIYA
410 420 430 440 450
LHHDPKYWTE PEKFCPERFS KKNKDSIDLY RYIPFGAGPR NCIGMRFALT
460 470 480 490 500
NIKLAVIRAL QNFSFKPCKE TQIPLKLDNL PILQPEKPIV LKVHLRDGIT
SGP
Isoform 4 (identifier: Q9HB55-4) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
224-250: SLFPFLTPVFEALNIGLFPKDVTHFLK → YRVSLCCLGRSAWCDLGSLKPPPPGFE
251-503: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.Curated
Show »Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PDL8 | E9PDL8_HUMAN | Cytochrome P450 3A43 | CYP3A43 | 292 | Annotation score: | ||
Q9HB50 | Q9HB50_HUMAN | Cytochrome P450 3A43 | CYP3A43 | 74 | Annotation score: | ||
E7EMH4 | E7EMH4_HUMAN | Cytochrome P450 3A43 | CYP3A43 | 96 | Annotation score: | ||
F8W6L8 | F8W6L8_HUMAN | Cytochrome P450 3A43 | CYP3A43 | 126 | Annotation score: | ||
C9JA26 | C9JA26_HUMAN | Cytochrome P450 3A43 | CYP3A43 | 167 | Annotation score: | ||
E9PG90 | E9PG90_HUMAN | Cytochrome P450 3A43 | CYP3A43 | 56 | Annotation score: |
Polymorphismi
At protein level, three alleles are known: CYP3A43*1, CYP3A43*2 and CYP3A43*3. The sequence shown is that of CYP3A43*1, which is the most frequent allele. The allele CYP3A43*2 is likely to be non-functional.
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_018050 | 25 – 88 | YGTHS…IMDPD → LGPIHINFLRSWEFLGQPLC LFWELFCSTLGVFGILTENV MKNTEKCGGCMRGNSPCWSS WIPT in allele CYP3A43*2. Add BLAST | 64 | |
Natural variantiVAR_048449 | 27 | T → A. Corresponds to variant dbSNP:rs45558032Ensembl. | 1 | |
Natural variantiVAR_018051 | 89 – 503 | Missing in allele CYP3A43*2. Add BLAST | 415 | |
Natural variantiVAR_048450 | 145 | M → I. Corresponds to variant dbSNP:rs45450092EnsemblClinVar. | 1 | |
Natural variantiVAR_048451 | 275 | M → I. Corresponds to variant dbSNP:rs45621431Ensembl. | 1 | |
Natural variantiVAR_018052 | 340 | P → A in allele CYP3A43*3. 1 PublicationCorresponds to variant dbSNP:rs680055Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056736 | 56 – 224 | GLWNF…LLLIS → RSLNKIPSWAWWLTPVIPAL WEAEAGGSPKVRSSRPALPT WVFGILTENVMKNTEKCGA in isoform 7. 1 PublicationAdd BLAST | 169 | |
Alternative sequenceiVSP_000612 | 224 – 250 | SLFPF…THFLK → YRVSLCCLGRSAWCDLGSLK PPPPGFE in isoform 4. CuratedAdd BLAST | 27 | |
Alternative sequenceiVSP_000613 | 251 – 503 | Missing in isoform 4. CuratedAdd BLAST | 253 | |
Alternative sequenceiVSP_000609 | 417 | E → ES in isoform 2. Curated | 1 | |
Alternative sequenceiVSP_000610 | 419 – 420 | FS → SH in isoform 3. Curated | 2 | |
Alternative sequenceiVSP_000611 | 421 – 503 | Missing in isoform 3. CuratedAdd BLAST | 83 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF319634 mRNA Translation: AAK00325.1 AF337813 mRNA Translation: AAK38841.1 AF280107 Genomic DNA Translation: AAG32291.1 AF280108 mRNA Translation: AAG33009.1 AF280109 mRNA Translation: AAG33010.1 AF280110 mRNA Translation: AAG33011.1 AF280111 mRNA Translation: AAG33012.1 AY390423 mRNA Translation: AAQ92351.1 AY390424 mRNA Translation: AAQ92352.1 AY390425 mRNA Translation: AAQ92353.1 AY390426 mRNA Translation: AAQ92354.1 AC011904 Genomic DNA Translation: AAS07394.1 AC011904 Genomic DNA Translation: AAS07395.1 CH471091 Genomic DNA Translation: EAW76632.1 CH471091 Genomic DNA Translation: EAW76634.1 BC100981 mRNA Translation: AAI00982.1 |
CCDSi | CCDS5675.1 [Q9HB55-2] CCDS5676.1 [Q9HB55-1] CCDS5677.1 [Q9HB55-3] CCDS64723.1 [Q9HB55-6] |
PIRi | JC7627 |
RefSeqi | NP_001265850.1, NM_001278921.1 [Q9HB55-6] NP_073731.1, NM_022820.4 [Q9HB55-2] NP_476436.1, NM_057095.2 [Q9HB55-1] NP_476437.1, NM_057096.3 [Q9HB55-3] |
Genome annotation databases
Ensembli | ENST00000222382; ENSP00000222382; ENSG00000021461 [Q9HB55-2] ENST00000312017; ENSP00000312110; ENSG00000021461 [Q9HB55-3] ENST00000354829; ENSP00000346887; ENSG00000021461 [Q9HB55-1] ENST00000417625; ENSP00000416581; ENSG00000021461 [Q9HB55-6] ENST00000434806; ENSP00000411653; ENSG00000021461 [Q9HB55-4] |
GeneIDi | 64816 |
KEGGi | hsa:64816 |
UCSCi | uc003urx.3, human [Q9HB55-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Web resourcesi
Cytochrome P450 Allele Nomenclature Committee CYP3A43 alleles |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF319634 mRNA Translation: AAK00325.1 AF337813 mRNA Translation: AAK38841.1 AF280107 Genomic DNA Translation: AAG32291.1 AF280108 mRNA Translation: AAG33009.1 AF280109 mRNA Translation: AAG33010.1 AF280110 mRNA Translation: AAG33011.1 AF280111 mRNA Translation: AAG33012.1 AY390423 mRNA Translation: AAQ92351.1 AY390424 mRNA Translation: AAQ92352.1 AY390425 mRNA Translation: AAQ92353.1 AY390426 mRNA Translation: AAQ92354.1 AC011904 Genomic DNA Translation: AAS07394.1 AC011904 Genomic DNA Translation: AAS07395.1 CH471091 Genomic DNA Translation: EAW76632.1 CH471091 Genomic DNA Translation: EAW76634.1 BC100981 mRNA Translation: AAI00982.1 |
CCDSi | CCDS5675.1 [Q9HB55-2] CCDS5676.1 [Q9HB55-1] CCDS5677.1 [Q9HB55-3] CCDS64723.1 [Q9HB55-6] |
PIRi | JC7627 |
RefSeqi | NP_001265850.1, NM_001278921.1 [Q9HB55-6] NP_073731.1, NM_022820.4 [Q9HB55-2] NP_476436.1, NM_057095.2 [Q9HB55-1] NP_476437.1, NM_057096.3 [Q9HB55-3] |
3D structure databases
SMRi | Q9HB55 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q9HB55, 1 interactor |
STRINGi | 9606.ENSP00000222382 |
Chemistry databases
BindingDBi | Q9HB55 |
ChEMBLi | CHEMBL5792 |
DrugBanki | DB11703, Acalabrutinib DB13141, Ambroxol acefyllinate DB12267, Brigatinib DB01222, Budesonide DB09065, Cobicistat DB12483, Copanlisib DB08865, Crizotinib DB09102, Daclatasvir DB01234, Dexamethasone DB14649, Dexamethasone acetate DB11994, Diacerein DB08930, Dolutegravir DB11979, Elagolix DB11574, Elbasvir DB00593, Ethosuximide DB00176, Fluvoxamine DB12307, Foretinib DB13879, Glecaprevir DB09054, Idelalisib DB11757, Istradefylline DB11951, Lemborexant DB12070, Letermovir DB01601, Lopinavir DB12130, Lorlatinib DB09212, Loxoprofen DB00643, Mebendazole DB00959, Methylprednisolone DB06595, Midostaurin DB11792, Mirodenafil DB09205, Moxisylyte DB11605, Myrrh DB11691, Naldemedine DB01149, Nefazodone DB09048, Netupitant DB00334, Olanzapine DB09568, Omega-3-carboxylic acids DB06412, Oxymetholone DB00780, Phenelzine DB13878, Pibrentasvir DB01058, Praziquantel DB14631, Prednisolone phosphate DB00635, Prednisone DB13174, Rhein DB01045, Rifampicin DB01201, Rifapentine DB12332, Rucaparib DB15685, Selpercatinib DB12887, Tazemetostat DB00624, Testosterone DB13943, Testosterone cypionate DB13944, Testosterone enanthate DB13946, Testosterone undecanoate DB00599, Thiopental DB13179, Troleandomycin DB11652, Tucatinib DB15035, Zanubrutinib |
DrugCentrali | Q9HB55 |
PTM databases
iPTMneti | Q9HB55 |
PhosphoSitePlusi | Q9HB55 |
Polymorphism and mutation databases
BioMutai | CYP3A43 |
DMDMi | 20137481 |
Proteomic databases
jPOSTi | Q9HB55 |
MassIVEi | Q9HB55 |
PaxDbi | Q9HB55 |
PeptideAtlasi | Q9HB55 |
PRIDEi | Q9HB55 |
ProteomicsDBi | 61978 81485 [Q9HB55-1] 81486 [Q9HB55-2] 81487 [Q9HB55-3] 81488 [Q9HB55-4] |
Protocols and materials databases
Antibodypediai | 30450, 126 antibodies |
Genome annotation databases
Ensembli | ENST00000222382; ENSP00000222382; ENSG00000021461 [Q9HB55-2] ENST00000312017; ENSP00000312110; ENSG00000021461 [Q9HB55-3] ENST00000354829; ENSP00000346887; ENSG00000021461 [Q9HB55-1] ENST00000417625; ENSP00000416581; ENSG00000021461 [Q9HB55-6] ENST00000434806; ENSP00000411653; ENSG00000021461 [Q9HB55-4] |
GeneIDi | 64816 |
KEGGi | hsa:64816 |
UCSCi | uc003urx.3, human [Q9HB55-1] |
Organism-specific databases
CTDi | 64816 |
DisGeNETi | 64816 |
EuPathDBi | HostDB:ENSG00000021461.16 |
GeneCardsi | CYP3A43 |
HGNCi | HGNC:17450, CYP3A43 |
HPAi | ENSG00000021461, Tissue enriched (liver) |
MIMi | 606534, gene |
neXtProti | NX_Q9HB55 |
OpenTargetsi | ENSG00000021461 |
PharmGKBi | PA427 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0158, Eukaryota |
GeneTreei | ENSGT00950000182958 |
HOGENOMi | CLU_001570_5_2_1 |
InParanoidi | Q9HB55 |
OMAi | CITWFGT |
OrthoDBi | 467733at2759 |
PhylomeDBi | Q9HB55 |
TreeFami | TF105087 |
Enzyme and pathway databases
PathwayCommonsi | Q9HB55 |
Reactomei | R-HSA-211958, Miscellaneous substrates R-HSA-211981, Xenobiotics |
Miscellaneous databases
BioGRID-ORCSi | 64816, 37 hits in 841 CRISPR screens |
GeneWikii | CYP3A43 |
GenomeRNAii | 64816 |
Pharosi | Q9HB55, Tclin |
PROi | PR:Q9HB55 |
RNActi | Q9HB55, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000021461, Expressed in body of pancreas and 111 other tissues |
ExpressionAtlasi | Q9HB55, baseline and differential |
Genevisiblei | Q9HB55, HS |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR008072, Cyt_P450_E_CYP3A IPR002402, Cyt_P450_E_grp-II IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00464, EP450II PR01689, EP450IICYP3A PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CP343_HUMAN | |
Accessioni | Q9HB55Primary (citable) accession number: Q9HB55 Secondary accession number(s): Q495Y1 Q9HB57 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 31, 2002 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 172 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations