UniProtKB - Q9HB20 (PKHA3_HUMAN)
Protein
Pleckstrin homology domain-containing family A member 3
Gene
PLEKHA3
Organism
Homo sapiens (Human)
Status
Functioni
Plays a role in regulation of vesicular cargo transport from the trans-Golgi network (TGN) to the plasma membrane (PubMed:15107860). Regulates Golgi phosphatidylinositol 4-phosphate (PtdIns4P) levels and activates the PtdIns4P phosphatase activity of SACM1L when it binds PtdIns4P in 'trans' configuration (PubMed:30659099). Binds preferentially to PtdIns4P (PubMed:11001876, PubMed:15107860). Negatively regulates APOB secretion from hepatocytes (PubMed:30659099).3 Publications
GO - Molecular functioni
- ceramide 1-phosphate binding Source: GO_Central
- ceramide 1-phosphate transfer activity Source: GO_Central
- identical protein binding Source: IntAct
- lipid binding Source: GO_Central
- phosphatidylinositol-4-phosphate binding Source: UniProtKB
GO - Biological processi
- ceramide transport Source: GO_Central
- intermembrane lipid transfer Source: GO_Central
- phosphatidylinositol biosynthetic process Source: Reactome
Keywordsi
Ligand | Lipid-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9HB20 |
Reactomei | R-HSA-1660499, Synthesis of PIPs at the plasma membrane |
Names & Taxonomyi
Protein namesi | Recommended name: Pleckstrin homology domain-containing family A member 3Short name: PH domain-containing family A member 3 Alternative name(s): Phosphatidylinositol-four-phosphate adapter protein 11 Publication Short name: FAPP-1 Short name: Phosphoinositol 4-phosphate adapter protein 1 |
Gene namesi | Name:PLEKHA3 Synonyms:FAPP1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000116095.10 |
HGNCi | HGNC:14338, PLEKHA3 |
MIMi | 607774, gene |
neXtProti | NX_Q9HB20 |
Subcellular locationi
Golgi apparatus
- trans-Golgi network membrane 1 Publication; Peripheral membrane protein 1 Publication
Note: Localizes to ER-trans Golgi network (TGN) contacts sites.1 Publication
Cytosol
- cytosol Source: GO_Central
Golgi apparatus
- Golgi apparatus Source: HPA
- Golgi membrane Source: Reactome
Other locations
- membrane Source: GO_Central
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 65977 |
OpenTargetsi | ENSG00000116095 |
PharmGKBi | PA33403 |
Miscellaneous databases
Pharosi | Q9HB20, Tbio |
Polymorphism and mutation databases
BioMutai | PLEKHA3 |
DMDMi | 48474646 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053878 | 1 – 300 | Pleckstrin homology domain-containing family A member 3Add BLAST | 300 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 236 | PhosphoserineCombined sources | 1 | |
Modified residuei | 244 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9HB20 |
jPOSTi | Q9HB20 |
MassIVEi | Q9HB20 |
MaxQBi | Q9HB20 |
PaxDbi | Q9HB20 |
PeptideAtlasi | Q9HB20 |
PRIDEi | Q9HB20 |
ProteomicsDBi | 81473 |
PTM databases
iPTMneti | Q9HB20 |
PhosphoSitePlusi | Q9HB20 |
Expressioni
Tissue specificityi
Widely expressed.
Gene expression databases
Bgeei | ENSG00000116095, Expressed in epithelial cell of pancreas and 216 other tissues |
ExpressionAtlasi | Q9HB20, baseline and differential |
Genevisiblei | Q9HB20, HS |
Organism-specific databases
HPAi | ENSG00000116095, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsQ9HB20
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 122427, 16 interactors |
IntActi | Q9HB20, 12 interactors |
STRINGi | 9606.ENSP00000234453 |
Miscellaneous databases
RNActi | Q9HB20, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q9HB20 |
SMRi | Q9HB20 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9HB20 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 93 | PHPROSITE-ProRule annotationAdd BLAST | 93 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 100 | Interaction with SACM1L1 PublicationAdd BLAST | 100 | |
Regioni | 97 – 300 | Interaction with VAPA and VAPB1 PublicationAdd BLAST | 204 |
Domaini
The PH domain binds the small GTPase ARF1 and phosphatidylinositol-4-phosphate (PtdIns4P) with high selectivity, and is required for its recruitment to the trans-Golgi network (TGN).1 Publication
Phylogenomic databases
eggNOGi | ENOG502QPTX, Eukaryota |
GeneTreei | ENSGT00940000155850 |
HOGENOMi | CLU_062751_0_0_1 |
InParanoidi | Q9HB20 |
OMAi | TDSCNDV |
OrthoDBi | 952122at2759 |
PhylomeDBi | Q9HB20 |
TreeFami | TF317467 |
Family and domain databases
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR011993, PH-like_dom_sf IPR001849, PH_domain |
Pfami | View protein in Pfam PF00169, PH, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9HB20-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEGVLYKWTN YLTGWQPRWF VLDNGILSYY DSQDDVCKGS KGSIKMAVCE
60 70 80 90 100
IKVHSADNTR MELIIPGEQH FYMKAVNAAE RQRWLVALGS SKACLTDTRT
110 120 130 140 150
KKEKEISETS ESLKTKMSEL RLYCDLLMQQ VHTIQEFVHH DENHSSPSAE
160 170 180 190 200
NMNEASSLLS ATCNTFITTL EECVKIANAK FKPEMFQLHH PDPLVSPVSP
210 220 230 240 250
SPVQMMKRSV SHPGSCSSER SSHSIKEPVS TLHRLSQRRR RTYSDTDSCS
260 270 280 290 300
DIPLEDPDRP VHCSKNTLNG DLASATIPEE SRLMAKKQSE SEDTLPSFSS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH7C0Q0 | H7C0Q0_HUMAN | Pleckstrin homology domain-containi... | PLEKHA3 | 114 | Annotation score: | ||
F8WCR7 | F8WCR7_HUMAN | Pleckstrin homology domain-containi... | PLEKHA3 | 61 | Annotation score: |
Sequence cautioni
The sequence BAA90927 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 284 | M → T in AAG15199 (PubMed:11001876).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF286162 mRNA Translation: AAG15199.1 AK000074 mRNA Translation: BAA90927.1 Different initiation. AK314459 mRNA Translation: BAG37067.1 AC009948 Genomic DNA Translation: AAX88884.1 CH471058 Genomic DNA Translation: EAX11026.1 BC044567 mRNA Translation: AAH44567.1 |
CCDSi | CCDS33336.1 |
RefSeqi | NP_061964.3, NM_019091.3 |
Genome annotation databases
Ensembli | ENST00000234453; ENSP00000234453; ENSG00000116095 |
GeneIDi | 65977 |
KEGGi | hsa:65977 |
UCSCi | uc002umn.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF286162 mRNA Translation: AAG15199.1 AK000074 mRNA Translation: BAA90927.1 Different initiation. AK314459 mRNA Translation: BAG37067.1 AC009948 Genomic DNA Translation: AAX88884.1 CH471058 Genomic DNA Translation: EAX11026.1 BC044567 mRNA Translation: AAH44567.1 |
CCDSi | CCDS33336.1 |
RefSeqi | NP_061964.3, NM_019091.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2KCJ | NMR | - | A | 1-100 | [»] | |
2MDX | NMR | - | A | 1-100 | [»] | |
3RCP | X-ray | 1.90 | A | 1-99 | [»] | |
BMRBi | Q9HB20 | |||||
SMRi | Q9HB20 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 122427, 16 interactors |
IntActi | Q9HB20, 12 interactors |
STRINGi | 9606.ENSP00000234453 |
PTM databases
iPTMneti | Q9HB20 |
PhosphoSitePlusi | Q9HB20 |
Polymorphism and mutation databases
BioMutai | PLEKHA3 |
DMDMi | 48474646 |
Proteomic databases
EPDi | Q9HB20 |
jPOSTi | Q9HB20 |
MassIVEi | Q9HB20 |
MaxQBi | Q9HB20 |
PaxDbi | Q9HB20 |
PeptideAtlasi | Q9HB20 |
PRIDEi | Q9HB20 |
ProteomicsDBi | 81473 |
Protocols and materials databases
Antibodypediai | 33940, 111 antibodies |
DNASUi | 65977 |
Genome annotation databases
Ensembli | ENST00000234453; ENSP00000234453; ENSG00000116095 |
GeneIDi | 65977 |
KEGGi | hsa:65977 |
UCSCi | uc002umn.4, human |
Organism-specific databases
CTDi | 65977 |
DisGeNETi | 65977 |
EuPathDBi | HostDB:ENSG00000116095.10 |
GeneCardsi | PLEKHA3 |
HGNCi | HGNC:14338, PLEKHA3 |
HPAi | ENSG00000116095, Low tissue specificity |
MIMi | 607774, gene |
neXtProti | NX_Q9HB20 |
OpenTargetsi | ENSG00000116095 |
PharmGKBi | PA33403 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QPTX, Eukaryota |
GeneTreei | ENSGT00940000155850 |
HOGENOMi | CLU_062751_0_0_1 |
InParanoidi | Q9HB20 |
OMAi | TDSCNDV |
OrthoDBi | 952122at2759 |
PhylomeDBi | Q9HB20 |
TreeFami | TF317467 |
Enzyme and pathway databases
PathwayCommonsi | Q9HB20 |
Reactomei | R-HSA-1660499, Synthesis of PIPs at the plasma membrane |
Miscellaneous databases
BioGRID-ORCSi | 65977, 9 hits in 844 CRISPR screens |
ChiTaRSi | PLEKHA3, human |
EvolutionaryTracei | Q9HB20 |
GenomeRNAii | 65977 |
Pharosi | Q9HB20, Tbio |
PROi | PR:Q9HB20 |
RNActi | Q9HB20, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000116095, Expressed in epithelial cell of pancreas and 216 other tissues |
ExpressionAtlasi | Q9HB20, baseline and differential |
Genevisiblei | Q9HB20, HS |
Family and domain databases
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR011993, PH-like_dom_sf IPR001849, PH_domain |
Pfami | View protein in Pfam PF00169, PH, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PKHA3_HUMAN | |
Accessioni | Q9HB20Primary (citable) accession number: Q9HB20 Secondary accession number(s): Q4ZG69, Q86TQ1, Q9NXT3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2004 |
Last sequence update: | March 29, 2004 | |
Last modified: | December 2, 2020 | |
This is version 150 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM