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Protein

Dual specificity protein kinase CLK4

Gene

CLK4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates SRSF1 and SRSF3. Required for the regulation of alternative splicing of MAPT/TAU. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Activity regulationi

TG003 inhibits its kinase activity and affects the regulation of alternative splicing mediated by phosphorylation of SR proteins.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei189ATPPROSITE-ProRule annotation1
Active sitei286Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi165 – 173ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • protein autophosphorylation Source: Ensembl
  • regulation of RNA splicing Source: UniProtKB

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ9HAZ1
SIGNORiQ9HAZ1

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein kinase CLK4 (EC:2.7.12.1)
Alternative name(s):
CDC-like kinase 4
Gene namesi
Name:CLK4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000113240.12
HGNCiHGNC:13659 CLK4
MIMi607969 gene
neXtProtiNX_Q9HAZ1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi189K → R: Loss of function. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000113240
PharmGKBiPA26598

Chemistry databases

ChEMBLiCHEMBL4203
GuidetoPHARMACOLOGYi1993

Polymorphism and mutation databases

BioMutaiCLK4
DMDMi34922132

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000858731 – 481Dual specificity protein kinase CLK4Add BLAST481

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei136PhosphoserineCombined sources1
Modified residuei138PhosphoserineCombined sources1

Post-translational modificationi

Autophosphorylates on all three types of residues.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9HAZ1
MaxQBiQ9HAZ1
PaxDbiQ9HAZ1
PeptideAtlasiQ9HAZ1
PRIDEiQ9HAZ1
ProteomicsDBi81459

PTM databases

iPTMnetiQ9HAZ1
PhosphoSitePlusiQ9HAZ1

Expressioni

Tissue specificityi

Expressed in liver, kidney, heart, muscle, brain and endothelial cells.2 Publications

Gene expression databases

BgeeiENSG00000113240 Expressed in 233 organ(s), highest expression level in right hemisphere of cerebellum
CleanExiHS_CLK4
ExpressionAtlasiQ9HAZ1 baseline and differential
GenevisibleiQ9HAZ1 HS

Organism-specific databases

HPAiHPA043746

Interactioni

Subunit structurei

Interacts with UBL5.2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi121501, 20 interactors
IntActiQ9HAZ1, 13 interactors
STRINGi9606.ENSP00000316948

Chemistry databases

BindingDBiQ9HAZ1

Structurei

Secondary structure

1481
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9HAZ1
SMRiQ9HAZ1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini159 – 475Protein kinasePROSITE-ProRule annotationAdd BLAST317

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0671 Eukaryota
ENOG410XQF2 LUCA
GeneTreeiENSGT00580000081366
HOGENOMiHOG000203417
HOVERGENiHBG107720
InParanoidiQ9HAZ1
KOiK08823
OMAiHGMDGIH
OrthoDBiEOG091G033A
PhylomeDBiQ9HAZ1
TreeFamiTF101041

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

Q9HAZ1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRHSKRTHCP DWDSRESWGH ESYRGSHKRK RRSHSSTQEN RHCKPHHQFK
60 70 80 90 100
ESDCHYLEAR SLNERDYRDR RYVDEYRNDY CEGYVPRHYH RDIESGYRIH
110 120 130 140 150
CSKSSVRSRR SSPKRKRNRH CSSHQSRSKS HRRKRSRSIE DDEEGHLICQ
160 170 180 190 200
SGDVLRARYE IVDTLGEGAF GKVVECIDHG MDGMHVAVKI VKNVGRYREA
210 220 230 240 250
ARSEIQVLEH LNSTDPNSVF RCVQMLEWFD HHGHVCIVFE LLGLSTYDFI
260 270 280 290 300
KENSFLPFQI DHIRQMAYQI CQSINFLHHN KLTHTDLKPE NILFVKSDYV
310 320 330 340 350
VKYNSKMKRD ERTLKNTDIK VVDFGSATYD DEHHSTLVST RHYRAPEVIL
360 370 380 390 400
ALGWSQPCDV WSIGCILIEY YLGFTVFQTH DSKEHLAMME RILGPIPQHM
410 420 430 440 450
IQKTRKRKYF HHNQLDWDEH SSAGRYVRRR CKPLKEFMLC HDEEHEKLFD
460 470 480
LVRRMLEYDP TQRITLDEAL QHPFFDLLKK K
Length:481
Mass (Da):57,492
Last modified:March 1, 2001 - v1
Checksum:iF402C36835CDA306
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B7ZL31B7ZL31_HUMAN
CLK4 protein
CLK4
373Annotation score:
A0A087WX74A0A087WX74_HUMAN
Dual-specificity protein kinase CLK...
CLK4
373Annotation score:
Q68D95Q68D95_HUMAN
Dual-specificity protein kinase CLK...
CLK4 DKFZp686A20267
53Annotation score:
E7EWJ6E7EWJ6_HUMAN
Dual-specificity protein kinase CLK...
CLK4
89Annotation score:
E7ES88E7ES88_HUMAN
Dual-specificity protein kinase CLK...
CLK4
143Annotation score:
Q6P090Q6P090_HUMAN
CLK4 protein
CLK4
54Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_040414352L → F1 PublicationCorresponds to variant dbSNP:rs35272416Ensembl.1
Natural variantiVAR_040415363I → V1 PublicationCorresponds to variant dbSNP:rs55746655Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF294429 mRNA Translation: AAG10074.1
CCDSiCCDS4437.1
RefSeqiNP_065717.1, NM_020666.2
UniGeneiHs.406557

Genome annotation databases

EnsembliENST00000316308; ENSP00000316948; ENSG00000113240
GeneIDi57396
KEGGihsa:57396
UCSCiuc003mjf.2 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF294429 mRNA Translation: AAG10074.1
CCDSiCCDS4437.1
RefSeqiNP_065717.1, NM_020666.2
UniGeneiHs.406557

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6FYVX-ray2.46A146-480[»]
ProteinModelPortaliQ9HAZ1
SMRiQ9HAZ1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121501, 20 interactors
IntActiQ9HAZ1, 13 interactors
STRINGi9606.ENSP00000316948

Chemistry databases

BindingDBiQ9HAZ1
ChEMBLiCHEMBL4203
GuidetoPHARMACOLOGYi1993

PTM databases

iPTMnetiQ9HAZ1
PhosphoSitePlusiQ9HAZ1

Polymorphism and mutation databases

BioMutaiCLK4
DMDMi34922132

Proteomic databases

EPDiQ9HAZ1
MaxQBiQ9HAZ1
PaxDbiQ9HAZ1
PeptideAtlasiQ9HAZ1
PRIDEiQ9HAZ1
ProteomicsDBi81459

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000316308; ENSP00000316948; ENSG00000113240
GeneIDi57396
KEGGihsa:57396
UCSCiuc003mjf.2 human

Organism-specific databases

CTDi57396
EuPathDBiHostDB:ENSG00000113240.12
GeneCardsiCLK4
HGNCiHGNC:13659 CLK4
HPAiHPA043746
MIMi607969 gene
neXtProtiNX_Q9HAZ1
OpenTargetsiENSG00000113240
PharmGKBiPA26598
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0671 Eukaryota
ENOG410XQF2 LUCA
GeneTreeiENSGT00580000081366
HOGENOMiHOG000203417
HOVERGENiHBG107720
InParanoidiQ9HAZ1
KOiK08823
OMAiHGMDGIH
OrthoDBiEOG091G033A
PhylomeDBiQ9HAZ1
TreeFamiTF101041

Enzyme and pathway databases

SignaLinkiQ9HAZ1
SIGNORiQ9HAZ1

Miscellaneous databases

ChiTaRSiCLK4 human
GenomeRNAii57396
PROiPR:Q9HAZ1
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000113240 Expressed in 233 organ(s), highest expression level in right hemisphere of cerebellum
CleanExiHS_CLK4
ExpressionAtlasiQ9HAZ1 baseline and differential
GenevisibleiQ9HAZ1 HS

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCLK4_HUMAN
AccessioniPrimary (citable) accession number: Q9HAZ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: March 1, 2001
Last modified: November 7, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  7. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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Main funding by: National Institutes of Health

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