UniProtKB - Q9HAW8 (UD110_HUMAN)
UDP-glucuronosyltransferase 1A10
UGT1A10
Functioni
Miscellaneous
Catalytic activityi
- EC:2.4.1.177 PublicationsThis reaction proceeds in the forward7 Publications direction.
- This reaction proceeds in the forward2 Publications direction.
- This reaction proceeds in the forward2 Publications direction.
- This reaction proceeds in the forward2 Publications direction.
- 16α,17β-estriol + UDP-α-D-glucuronate = 16α,17β-estriol 3-O-(β-D-glucuronate) + H+ + UDP1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 16β,17β-estriol + UDP-α-D-glucuronate = 16β,17β-estriol 3-O-(β-D-glucuronate) + H+ + UDP1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 16α,17α-estriol + UDP-α-D-glucuronate = 16α,17α-estriol 3-O-(β-D-glucuronate) + H+ + UDP1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 16α-hydroxyestrone + UDP-α-D-glucuronate = 16α-hydroxyestrone 3-O-(β-D-glucuronate) + H+ + UDP1 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Kineticsi
- KM=13 µM for estrone (when assaying glucuronidation at position 3)1 Publication
- KM=4.07 µM for 17beta-estradiol/estradiol (when assaying glucuronidation at position 3)1 Publication
- KM=2.63 µM for 17beta-estradiol/estradiol (when assaying glucuronidation at position 17)1 Publication
- KM=21.8 µM for 17alpha-estradiol/epiestradiol (when assaying glucuronidation at position 3)1 Publication
- KM=68.4 µM for 16alpha,17beta-estriol/estriol (when assaying glucuronidation at position 3)1 Publication
- KM=59.8 µM for 16beta,17beta-estriol (when assaying glucuronidation at position 3)1 Publication
- KM=337 µM for 16alpha,17alpha-estriol (when assaying glucuronidation at position 3)1 Publication
- KM=12.34 µM for genistein1 Publication
- KM=31.5 µM for SN-38 (when assaying glucuronidation at position 10)1 Publication
- Vmax=1300 pmol/min/mg enzyme for the formation of estrone 3-O-(beta-D-glucuronate)1 Publication
- Vmax=6340 pmol/min/mg enzyme for the formation of 17beta-estradiol 3-O-(beta-D-glucuronate)1 Publication
- Vmax=83.6 pmol/min/mg enzyme for the formation of 17beta-estradiol 17-O-(beta-D-glucuronate)1 Publication
- Vmax=2100 pmol/min/mg enzyme for the formation of 17alpha-estradiol 3-O-(beta-D-glucuronate)1 Publication
- Vmax=3040 pmol/min/mg enzyme for the formation of 17beta-estradiol 3-O-(beta-D-glucuronate)1 Publication
- Vmax=107 pmol/min/mg enzyme for the formation of 17beta-estradiol 17-O-(beta-D-glucuronate)1 Publication
- Vmax=321 pmol/min/mg enzyme for the formation of 17alpha-estradiol 3-O-(beta-D-glucuronate)1 Publication
- Vmax=8.5 pmol/min/mg enzyme for the formation of 17alpha-estradiol 17-O-(beta-D-glucuronate)1 Publication
- Vmax=1200 pmol/min/mg enzyme for the formation of 16alpha,17beta-estriol 3-O-(beta-D-glucuronate)1 Publication
- Vmax=837 pmol/min/mg enzyme for the formation of 16alpha,17beta-estriol 3-O-(beta-D-glucuronate)1 Publication
- Vmax=7700 pmol/min/mg enzyme for the formation of 16beta,17beta-estriol 3-O-(beta-D-glucuronate)1 Publication
- Vmax=3486 pmol/min/mg enzyme for the formation of 16beta,17beta-estriol 3-O-(beta-D-glucuronate)1 Publication
- Vmax=958 pmol/min/mg enzyme for the formation of 16alpha,17alpha-estriol 3-O-(beta-D-glucuronate)1 Publication
- Vmax=407 pmol/min/mg enzyme for the formation of 16alpha,17alpha-estriol 3-O-(beta-D-glucuronate)1 Publication
- Vmax=3 pmol/min/mg enzyme for the formation of 17beta-estradiol 3-O-(beta-D-glucuronate)1 Publication
- Vmax=3 pmol/min/mg enzyme for the formation of estrone 3-O-(beta-D-glucuronate)1 Publication
- Vmax=11 pmol/min/mg enzyme for the formation of 2-methoxy-17beta-estradiol 3-O-(beta-D-glucuronate)1 Publication
- Vmax=1.04 nmol/min/mg enzyme for the formation of genistein glucuronide1 Publication
- Vmax=0.25 nmol/min/mg enzyme for the formation of prunetin-4'-O-(beta-D-glucuronoside)1 Publication
- Vmax=7.6 pmol/min/mg enzyme for the formation of SN-38 glucuronide1 Publication
GO - Molecular functioni
- enzyme binding Source: BHF-UCL
- glucuronosyltransferase activity Source: BHF-UCL
- protein heterodimerization activity Source: BHF-UCL
- protein homodimerization activity Source: UniProtKB
- protein kinase C binding Source: BHF-UCL
GO - Biological processi
- cellular glucuronidation Source: BHF-UCL
- flavone metabolic process Source: BHF-UCL
- flavonoid glucuronidation Source: GO_Central
- negative regulation of cellular glucuronidation Source: BHF-UCL
- negative regulation of fatty acid metabolic process Source: BHF-UCL
- negative regulation of glucuronosyltransferase activity Source: BHF-UCL
- xenobiotic glucuronidation Source: GO_Central
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
BRENDAi | 2.4.1.17, 2681 |
PathwayCommonsi | Q9HAW8 |
Reactomei | R-HSA-156588, Glucuronidation |
SABIO-RKi | Q9HAW8 |
SIGNORi | Q9HAW8 |
Protein family/group databases
CAZyi | GT1, Glycosyltransferase Family 1 |
Chemistry databases
SwissLipidsi | SLP:000001694 |
Names & Taxonomyi
Protein namesi | Recommended name: UDP-glucuronosyltransferase 1A101 Publication (EC:2.4.1.177 Publications)Short name: UGT1A10 Alternative name(s): UDP-glucuronosyltransferase 1-10 Short name: UDPGT 1-10 Short name: UGT1*10 Short name: UGT1-10 Short name: UGT1.10 UDP-glucuronosyltransferase 1-J Short name: UGT-1J Short name: UGT1J |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:12531, UGT1A10 |
MIMi | 191740, gene 606435, gene |
neXtProti | NX_Q9HAW8 |
VEuPathDBi | HostDB:ENSG00000242515.5 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Single-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- endoplasmic reticulum membrane Source: Reactome
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 488 – 504 | HelicalSequence analysisAdd BLAST | 17 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 90 | F → G, A, V, L, I or Y: Loss of estrone and 16alpha-hydroxyestrone glucuronosyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 93 | F → G: Increased estriol, 17-epiestriol and 16alpha-hydroxyestrone glucuronosyltransferase activity. Decreased of estrone and 16-epiestriol glucuronosyltransferase activity. 2 Publications | 1 | |
Mutagenesisi | 93 | F → V, L or I: Loss of estrone and 16alpha-hydroxyestrone glucuronosyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 93 | F → Y: Decreased estrone and 16alpha-hydroxyestrone glucuronosyltransferase activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 54575 |
OpenTargetsi | ENSG00000242515 |
PharmGKBi | PA37174 |
Miscellaneous databases
Pharosi | Q9HAW8, Tbio |
Chemistry databases
ChEMBLi | CHEMBL1743320 |
DrugBanki | DB00714, Apomorphine DB06401, Bazedoxifene DB14635, Curcumin sulfate DB00783, Estradiol DB00749, Etodolac DB00555, Lamotrigine DB00678, Losartan DB00688, Mycophenolate mofetil DB00788, Naproxen DB09079, Nintedanib DB00794, Primidone DB09288, Propacetamol DB00481, Raloxifene DB00503, Ritonavir DB00675, Tamoxifen DB00197, Troglitazone DB00313, Valproic acid |
DrugCentrali | Q9HAW8 |
Genetic variation databases
BioMutai | UGT1A10 |
DMDMi | 29839636 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | Sequence analysisAdd BLAST | 25 | |
ChainiPRO_0000036009 | 26 – 530 | UDP-glucuronosyltransferase 1A10Add BLAST | 505 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 71 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 292 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 344 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
jPOSTi | Q9HAW8 |
MassIVEi | Q9HAW8 |
MaxQBi | Q9HAW8 |
PaxDbi | Q9HAW8 |
PeptideAtlasi | Q9HAW8 |
PRIDEi | Q9HAW8 |
ProteomicsDBi | 69409 81455 [Q9HAW8-1] |
PTM databases
GlyConnecti | 1874, 2 N-Linked glycans (1 site) |
GlyGeni | Q9HAW8, 3 sites |
iPTMneti | Q9HAW8 |
PhosphoSitePlusi | Q9HAW8 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000242515, Expressed in intestine and 50 other tissues |
ExpressionAtlasi | Q9HAW8, baseline and differential |
Genevisiblei | Q9HAW8, HS |
Organism-specific databases
HPAi | ENSG00000242515, Group enriched (esophagus, gallbladder, intestine, stomach) |
Interactioni
Subunit structurei
Homodimer (PubMed:17179145). Homooligomer (Probable).
Interacts with UGT1A1, UGT1A3, UGT1A4, UGT1A6, UGT1A7, UGT1A8 and UGT1A9 to form heterodimers (PubMed:17179145).
Isoform 1 interacts with isoform 2/i2 suggesting that oligomerization is involved in negative regulation of transferase activity by isoform 2. Isoform 1 also interacts with respective i2 isoforms of UGT1A1, UGT1A3, UGT1A4, UGT1A6, UGT1A7, UGT1A8 and UGT1A9 (PubMed:20610558).
1 Publication2 PublicationsGO - Molecular functioni
- enzyme binding Source: BHF-UCL
- protein heterodimerization activity Source: BHF-UCL
- protein homodimerization activity Source: UniProtKB
- protein kinase C binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 120053, 21 interactors |
IntActi | Q9HAW8, 20 interactors |
STRINGi | 9606.ENSP00000343838 |
Chemistry databases
BindingDBi | Q9HAW8 |
Miscellaneous databases
RNActi | Q9HAW8, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1192, Eukaryota |
GeneTreei | ENSGT00940000163976 |
HOGENOMi | CLU_012949_3_1_1 |
InParanoidi | Q9HAW8 |
OMAi | ATTRSIC |
PhylomeDBi | Q9HAW8 |
TreeFami | TF315472 |
Family and domain databases
InterProi | View protein in InterPro IPR002213, UDP_glucos_trans IPR035595, UDP_glycos_trans_CS |
Pfami | View protein in Pfam PF00201, UDPGT, 1 hit |
PROSITEi | View protein in PROSITE PS00375, UDPGT, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MARAGWTSPV PLCVCLLLTC GFAEAGKLLV VPMDGSHWFT MQSVVEKLIL
60 70 80 90 100
RGHEVVVVMP EVSWQLERSL NCTVKTYSTS YTLEDQNREF MVFAHAQWKA
110 120 130 140 150
QAQSIFSLLM SSSSGFLDLF FSHCRSLFND RKLVEYLKES SFDAVFLDPF
160 170 180 190 200
DTCGLIVAKY FSLPSVVFTR GIFCHHLEEG AQCPAPLSYV PNDLLGFSDA
210 220 230 240 250
MTFKERVWNH IVHLEDHLFC QYLFRNALEI ASEILQTPVT AYDLYSHTSI
260 270 280 290 300
WLLRTDFVLD YPKPVMPNMI FIGGINCHQG KPLPMEFEAY INASGEHGIV
310 320 330 340 350
VFSLGSMVSE IPEKKAMAIA DALGKIPQTV LWRYTGTRPS NLANNTILVK
360 370 380 390 400
WLPQNDLLGH PMTRAFITHA GSHGVYESIC NGVPMVMMPL FGDQMDNAKR
410 420 430 440 450
METKGAGVTL NVLEMTSEDL ENALKAVIND KSYKENIMRL SSLHKDRPVE
460 470 480 490 500
PLDLAVFWVE FVMRHKGAPH LRPAAHDLTW YQYHSLDVIG FLLAVVLTVA
510 520 530
FITFKCCAYG YRKCLGKKGR VKKAHKSKTH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1 – 23 | MARAG…TCGFA → MAPRRVDQPRSFMCVSTADL WLC (PubMed:9271343).CuratedAdd BLAST | 23 | |
Sequence conflicti | 40 | T → A in AAB81537 (PubMed:9271343).Curated | 1 | |
Sequence conflicti | 175 | H → R in AAB81537 (PubMed:9271343).Curated | 1 | |
Sequence conflicti | 224 | F → L in AAB81537 (PubMed:9271343).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_018354 | 59 | M → I1 PublicationCorresponds to variant dbSNP:rs56935833Ensembl. | 1 | |
Natural variantiVAR_052464 | 139 | E → K. Corresponds to variant dbSNP:rs10187694Ensembl. | 1 | |
Natural variantiVAR_018355 | 202 | T → I1 PublicationCorresponds to variant dbSNP:rs58704432Ensembl. | 1 | |
Natural variantiVAR_052465 | 244 | L → I. Corresponds to variant dbSNP:rs28969685Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_053966 | 432 – 530 | SYKEN…KSKTH → RKKQQSGRQM in isoform 2. 1 PublicationAdd BLAST | 99 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U89508 mRNA Translation: AAB81537.1 AF297093 Genomic DNA Translation: AAG30417.1 AC006985 Genomic DNA No translation available. AC019072 Genomic DNA No translation available. BC020971 mRNA Translation: AAH20971.1 BC053576 mRNA Translation: AAH53576.1 BC069210 mRNA Translation: AAH69210.2 |
CCDSi | CCDS33403.1 [Q9HAW8-1] |
PIRi | JC5656 |
RefSeqi | NP_061948.1, NM_019075.2 [Q9HAW8-1] |
Genome annotation databases
Ensembli | ENST00000344644; ENSP00000343838; ENSG00000242515 [Q9HAW8-1] ENST00000373445; ENSP00000362544; ENSG00000242515 [Q9HAW8-2] |
GeneIDi | 54575 |
KEGGi | hsa:54575 |
UCSCi | uc002vuq.4, human [Q9HAW8-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U89508 mRNA Translation: AAB81537.1 AF297093 Genomic DNA Translation: AAG30417.1 AC006985 Genomic DNA No translation available. AC019072 Genomic DNA No translation available. BC020971 mRNA Translation: AAH20971.1 BC053576 mRNA Translation: AAH53576.1 BC069210 mRNA Translation: AAH69210.2 |
CCDSi | CCDS33403.1 [Q9HAW8-1] |
PIRi | JC5656 |
RefSeqi | NP_061948.1, NM_019075.2 [Q9HAW8-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 120053, 21 interactors |
IntActi | Q9HAW8, 20 interactors |
STRINGi | 9606.ENSP00000343838 |
Chemistry databases
BindingDBi | Q9HAW8 |
ChEMBLi | CHEMBL1743320 |
DrugBanki | DB00714, Apomorphine DB06401, Bazedoxifene DB14635, Curcumin sulfate DB00783, Estradiol DB00749, Etodolac DB00555, Lamotrigine DB00678, Losartan DB00688, Mycophenolate mofetil DB00788, Naproxen DB09079, Nintedanib DB00794, Primidone DB09288, Propacetamol DB00481, Raloxifene DB00503, Ritonavir DB00675, Tamoxifen DB00197, Troglitazone DB00313, Valproic acid |
DrugCentrali | Q9HAW8 |
SwissLipidsi | SLP:000001694 |
Protein family/group databases
CAZyi | GT1, Glycosyltransferase Family 1 |
PTM databases
GlyConnecti | 1874, 2 N-Linked glycans (1 site) |
GlyGeni | Q9HAW8, 3 sites |
iPTMneti | Q9HAW8 |
PhosphoSitePlusi | Q9HAW8 |
Genetic variation databases
BioMutai | UGT1A10 |
DMDMi | 29839636 |
Proteomic databases
jPOSTi | Q9HAW8 |
MassIVEi | Q9HAW8 |
MaxQBi | Q9HAW8 |
PaxDbi | Q9HAW8 |
PeptideAtlasi | Q9HAW8 |
PRIDEi | Q9HAW8 |
ProteomicsDBi | 69409 81455 [Q9HAW8-1] |
Protocols and materials databases
Antibodypediai | 35063, 30 antibodies |
DNASUi | 54575 |
Genome annotation databases
Ensembli | ENST00000344644; ENSP00000343838; ENSG00000242515 [Q9HAW8-1] ENST00000373445; ENSP00000362544; ENSG00000242515 [Q9HAW8-2] |
GeneIDi | 54575 |
KEGGi | hsa:54575 |
UCSCi | uc002vuq.4, human [Q9HAW8-1] |
Organism-specific databases
CTDi | 54575 |
DisGeNETi | 54575 |
GeneCardsi | UGT1A10 |
HGNCi | HGNC:12531, UGT1A10 |
HPAi | ENSG00000242515, Group enriched (esophagus, gallbladder, intestine, stomach) |
MIMi | 191740, gene 606435, gene |
neXtProti | NX_Q9HAW8 |
OpenTargetsi | ENSG00000242515 |
PharmGKBi | PA37174 |
VEuPathDBi | HostDB:ENSG00000242515.5 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1192, Eukaryota |
GeneTreei | ENSGT00940000163976 |
HOGENOMi | CLU_012949_3_1_1 |
InParanoidi | Q9HAW8 |
OMAi | ATTRSIC |
PhylomeDBi | Q9HAW8 |
TreeFami | TF315472 |
Enzyme and pathway databases
BRENDAi | 2.4.1.17, 2681 |
PathwayCommonsi | Q9HAW8 |
Reactomei | R-HSA-156588, Glucuronidation |
SABIO-RKi | Q9HAW8 |
SIGNORi | Q9HAW8 |
Miscellaneous databases
BioGRID-ORCSi | 54575, 0 hits in 798 CRISPR screens |
GeneWikii | UGT1A10 |
GenomeRNAii | 54575 |
Pharosi | Q9HAW8, Tbio |
PROi | PR:Q9HAW8 |
RNActi | Q9HAW8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000242515, Expressed in intestine and 50 other tissues |
ExpressionAtlasi | Q9HAW8, baseline and differential |
Genevisiblei | Q9HAW8, HS |
Family and domain databases
InterProi | View protein in InterPro IPR002213, UDP_glucos_trans IPR035595, UDP_glycos_trans_CS |
Pfami | View protein in Pfam PF00201, UDPGT, 1 hit |
PROSITEi | View protein in PROSITE PS00375, UDPGT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UD110_HUMAN | |
Accessioni | Q9HAW8Primary (citable) accession number: Q9HAW8 Secondary accession number(s): O00474, Q6NT91, Q7Z6H8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 11, 2003 |
Last sequence update: | March 1, 2001 | |
Last modified: | February 10, 2021 | |
This is version 175 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families