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Protein

E3 ubiquitin-protein ligase pellino homolog 2

Gene

PELI2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates IL1B-induced IRAK1 'Lys-63'-linked polyubiquitination and possibly 'Lys-48'-linked ubiquitination. May be important for LPS- and IL1B-induced MAP3K7-dependent, but not MAP3K3-dependent, NF-kappa-B activation. Can activate the MAP (mitogen activated protein) kinase pathway leading to activation of ELK1.5 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-9020702 Interleukin-1 signaling
R-HSA-937039 IRAK1 recruits IKK complex
R-HSA-975144 IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation
SIGNORiQ9HAT8
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase pellino homolog 2 (EC:2.3.2.27)
Short name:
Pellino-2
Alternative name(s):
RING-type E3 ubiquitin transferase pellino homolog 2Curated
Gene namesi
Name:PELI2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000139946.9
HGNCiHGNC:8828 PELI2
MIMi614798 gene
neXtProtiNX_Q9HAT8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi106R → A: Abolishes binding to IRAK1. 1 Publication1
Mutagenesisi187T → A: Abolishes binding to IRAK1; when associated with A-188. 1 Publication1
Mutagenesisi188N → A: Abolishes binding to IRAK1; when associated with A-187. 1 Publication1
Mutagenesisi397C → A: Loss of IRAK1-polyubiquitination. 1 Publication1
Mutagenesisi400C → A: Loss of IRAK1-polyubiquitination. 1 Publication1

Organism-specific databases

DisGeNETi57161
OpenTargetsiENSG00000139946
PharmGKBiPA33173

Polymorphism and mutation databases

BioMutaiPELI2
DMDMi37999785

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941741 – 420E3 ubiquitin-protein ligase pellino homolog 2Add BLAST420

Post-translational modificationi

Phosphorylated by IRAK1 and IRAK4 enhancing its E3 ligase activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9HAT8
MaxQBiQ9HAT8
PaxDbiQ9HAT8
PeptideAtlasiQ9HAT8
PRIDEiQ9HAT8
ProteomicsDBi81433

PTM databases

iPTMnetiQ9HAT8
PhosphoSitePlusiQ9HAT8

Expressioni

Gene expression databases

BgeeiENSG00000139946
CleanExiHS_PELI2
ExpressionAtlasiQ9HAT8 baseline and differential
GenevisibleiQ9HAT8 HS

Organism-specific databases

HPAiHPA053182

Interactioni

Subunit structurei

Interacts with TRAF6, IRAK1, IRAK4 and MAP3K7. Interacts with BCL10; this interaction is impaired by SOCS3 (By similarity).By similarity

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi121417, 19 interactors
DIPiDIP-31350N
IntActiQ9HAT8, 32 interactors
MINTiQ9HAT8
STRINGi9606.ENSP00000267460

Structurei

Secondary structure

1420
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi18 – 24Combined sources7
Beta strandi41 – 46Combined sources6
Beta strandi53 – 63Combined sources11
Beta strandi76 – 82Combined sources7
Beta strandi84 – 96Combined sources13
Beta strandi99 – 106Combined sources8
Beta strandi113 – 115Combined sources3
Beta strandi141 – 148Combined sources8
Beta strandi153 – 158Combined sources6
Beta strandi164 – 168Combined sources5
Beta strandi174 – 176Combined sources3
Beta strandi182 – 184Combined sources3
Beta strandi190 – 193Combined sources4
Beta strandi208 – 211Combined sources4
Beta strandi215 – 218Combined sources4
Beta strandi222 – 225Combined sources4
Beta strandi245 – 247Combined sources3
Beta strandi252 – 257Combined sources6
Helixi258 – 263Combined sources6
Helixi266 – 269Combined sources4
Helixi270 – 276Combined sources7

3D structure databases

ProteinModelPortaliQ9HAT8
SMRiQ9HAT8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HAT8

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 202FHA; atypicalAdd BLAST188

Domaini

The atypical FHA domain contains a 'wing' insert and mediates binding to threonine-phosphorylated IRAK1.1 Publication

Sequence similaritiesi

Belongs to the pellino family.Curated

Phylogenomic databases

eggNOGiKOG3842 Eukaryota
ENOG410XP3S LUCA
GeneTreeiENSGT00390000007680
HOGENOMiHOG000234110
HOVERGENiHBG053559
InParanoidiQ9HAT8
KOiK11964
OMAiESKPGVW
OrthoDBiEOG091G0631
PhylomeDBiQ9HAT8
TreeFamiTF314338

Family and domain databases

InterProiView protein in InterPro
IPR006800 Pellino_fam
PANTHERiPTHR12098 PTHR12098, 1 hit
PfamiView protein in Pfam
PF04710 Pellino, 1 hit
PIRSFiPIRSF038886 Pellino, 1 hit

Sequencei

Sequence statusi: Complete.

Q9HAT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSPGQEEHC APNKEPVKYG ELVVLGYNGA LPNGDRGRRK SRFALYKRPK
60 70 80 90 100
ANGVKPSTVH VISTPQASKA ISCKGQHSIS YTLSRNQTVV VEYTHDKDTD
110 120 130 140 150
MFQVGRSTES PIDFVVTDTI SGSQNTDEAQ ITQSTISRFA CRIVCDRNEP
160 170 180 190 200
YTARIFAAGF DSSKNIFLGE KAAKWKNPDG HMDGLTTNGV LVMHPRGGFT
210 220 230 240 250
EESQPGVWRE ISVCGDVYTL RETRSAQQRG KLVESETNVL QDGSLIDLCG
260 270 280 290 300
ATLLWRTADG LFHTPTQKHI EALRQEINAA RPQCPVGLNT LAFPSINRKE
310 320 330 340 350
VVEEKQPWAY LSCGHVHGYH NWGHRSDTEA NERECPMCRT VGPYVPLWLG
360 370 380 390 400
CEAGFYVDAG PPTHAFTPCG HVCSEKSAKY WSQIPLPHGT HAFHAACPFC
410 420
ATQLVGEQNC IKLIFQGPID
Length:420
Mass (Da):46,435
Last modified:March 1, 2001 - v1
Checksum:i2FC5E661C13BC11A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302502 mRNA Translation: AAG15390.1
AK315727 mRNA Translation: BAG38082.1
CH471061 Genomic DNA Translation: EAW80689.1
BC009476 mRNA Translation: AAH09476.1
CCDSiCCDS9726.1
RefSeqiNP_067078.1, NM_021255.2
UniGeneiHs.657926

Genome annotation databases

EnsembliENST00000267460; ENSP00000267460; ENSG00000139946
GeneIDi57161
KEGGihsa:57161
UCSCiuc001xch.4 human

Similar proteinsi

Entry informationi

Entry nameiPELI2_HUMAN
AccessioniPrimary (citable) accession number: Q9HAT8
Secondary accession number(s): B2RDY5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: March 1, 2001
Last modified: July 18, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

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