UniProtKB - Q9HAP2 (MLXIP_HUMAN)
MLX-interacting protein
MLXIP
Functioni
Binds DNA as a heterodimer with MLX and activates transcription. Binds to the canonical E box sequence 5'-CACGTG-3'. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.
By similarity2 PublicationsGO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
GO - Biological processi
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q9HAP2 |
SignaLinki | Q9HAP2 |
Names & Taxonomyi
Protein namesi | Recommended name: MLX-interacting proteinAlternative name(s): Class E basic helix-loop-helix protein 36 Short name: bHLHe36 Transcriptional activator MondoA |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:17055, MLXIP |
MIMi | 608090, gene |
neXtProti | NX_Q9HAP2 |
VEuPathDBi | HostDB:ENSG00000175727 |
Subcellular locationi
Mitochondrion
- Mitochondrion outer membrane 2 Publications
Nucleus
- Nucleus PROSITE-ProRule annotation2 Publications
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Note: Predominantly cytoplasmic but shuttles between cytoplasm and nucleus when associated with MLX. Also associates with the outer mitochondrial membrane and may shuttle between the outer mitochondrial membrane and the nucleus.By similarity2 Publications
Mitochondrion
- mitochondrial outer membrane Source: UniProtKB-SubCell
Nucleus
- nucleus Source: GO_Central
Other locations
- chromatin Source: NTNU_SB
Keywords - Cellular componenti
Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 22877 |
OpenTargetsi | ENSG00000175727 |
PharmGKBi | PA128394590 |
Miscellaneous databases
Pharosi | Q9HAP2, Tbio |
Genetic variation databases
BioMutai | MLXIP |
DMDMi | 156632588 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000298763 | 2 – 919 | MLX-interacting proteinAdd BLAST | 918 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 9 | PhosphoserineCombined sources | 1 | |
Modified residuei | 27 | PhosphoserineCombined sources | 1 | |
Modified residuei | 33 | PhosphoserineCombined sources | 1 | |
Modified residuei | 39 | PhosphoserineBy similarity | 1 | |
Modified residuei | 669 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9HAP2 |
jPOSTi | Q9HAP2 |
MassIVEi | Q9HAP2 |
MaxQBi | Q9HAP2 |
PaxDbi | Q9HAP2 |
PeptideAtlasi | Q9HAP2 |
PRIDEi | Q9HAP2 |
ProteomicsDBi | 81411 [Q9HAP2-1] 81412 [Q9HAP2-2] 81413 [Q9HAP2-3] 81414 [Q9HAP2-4] 81415 [Q9HAP2-5] |
PTM databases
GlyGeni | Q9HAP2, 8 sites, 2 O-linked glycans (8 sites) |
iPTMneti | Q9HAP2 |
PhosphoSitePlusi | Q9HAP2 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000175727, Expressed in caecum and 234 other tissues |
ExpressionAtlasi | Q9HAP2, baseline and differential |
Genevisiblei | Q9HAP2, HS |
Organism-specific databases
HPAi | ENSG00000175727, Tissue enhanced (skeletal) |
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer with MLX.
By similarity1 PublicationGO - Molecular functioni
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 116544, 15 interactors |
IntActi | Q9HAP2, 10 interactors |
STRINGi | 9606.ENSP00000312834 |
Miscellaneous databases
RNActi | Q9HAP2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 719 – 769 | bHLHPROSITE-ProRule annotationAdd BLAST | 51 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 72 | DisorderedSequence analysisAdd BLAST | 72 | |
Regioni | 73 – 327 | Required for cytoplasmic localization1 PublicationAdd BLAST | 255 | |
Regioni | 322 – 445 | Transactivation domain1 PublicationAdd BLAST | 124 | |
Regioni | 542 – 562 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 633 – 712 | DisorderedSequence analysisAdd BLAST | 80 | |
Regioni | 769 – 790 | Leucine-zipperAdd BLAST | 22 | |
Regioni | 832 – 881 | Mediates heterotypic interactions between MLXIP and MLX and is required for cytoplasmic localization1 PublicationAdd BLAST | 50 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 668 – 710 | Polar residuesSequence analysisAdd BLAST | 43 |
Phylogenomic databases
eggNOGi | KOG3582, Eukaryota |
GeneTreei | ENSGT00940000158691 |
HOGENOMi | CLU_007471_1_0_1 |
InParanoidi | Q9HAP2 |
OMAi | QTCQTYQ |
OrthoDBi | 388166at2759 |
PhylomeDBi | Q9HAP2 |
TreeFami | TF324749 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR032648, MLXIP |
PANTHERi | PTHR15741:SF23, PTHR15741:SF23, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAADVFMCSP RRPRSRGRQV LLKPQVSEDD DDSDTDEPSP PPASGAATPA
60 70 80 90 100
RAHASAAPPP PRAGPGREEP PRRQQIIHSG HFMVSSPHRE HPPKKGYDFD
110 120 130 140 150
TVNKQTCQTY SFGKTSSCHL SIDASLTKLF ECMTLAYSGK LVSPKWKNFK
160 170 180 190 200
GLKLQWRDKI RLNNAIWRAW YMQYLEKRKN PVCHFVTPLD GSVDVDEHRR
210 220 230 240 250
PEAITTEGKY WKSRIEIVIR EYHKWRTYFK KRLQQHKDED LSSLVQDDDM
260 270 280 290 300
LYWHKHGDGW KTPVPMEEDP LLDTDMLMSE FSDTLFSTLS SHQPVAWPNP
310 320 330 340 350
REIAHLGNAD MIQPGLIPLQ PNLDFMDTFE PFQDLFSSSR SIFGSMLPAS
360 370 380 390 400
ASAPVPDPNN PPAQESILPT TALPTVSLPD SLIAPPTAPS LAHMDEQGCE
410 420 430 440 450
HTSRTEDPFI QPTDFGPSEP PLSVPQPFLP VFTMPLLSPS PAPPPISPVL
460 470 480 490 500
PLVPPPATAL NPPAPPTFHQ PQKFAGVNKA PSVITHTASA TLTHDAPATT
510 520 530 540 550
FSQSQGLVIT THHPAPSAAP CGLALSPVTR PPQPRLTFVH PKPVSLTGGR
560 570 580 590 600
PKQPHKIVPA PKPEPVSLVL KNARIAPAAF SGQPQAVIMT SGPLKREGML
610 620 630 640 650
ASTVSQSNVV IAPAAIARAP GVPEFHSSIL VTDLGHGTSS PPAPVSRLFP
660 670 680 690 700
STAQDPLGKG EQVPLHGGSP QVTVTGPSRD CPNSGQASPC ASEQSPSPQS
710 720 730 740 750
PQNNCSGKSD PKNVAALKNR QMKHISAEQK RRFNIKMCFD MLNSLISNNS
760 770 780 790 800
KLTSHAITLQ KTVEYITKLQ QERGQMQEEA RRLREEIEEL NATIISCQQL
810 820 830 840 850
LPATGVPVTR RQFDHMKDMF DEYVKTRTLQ NWKFWIFSII IKPLFESFKG
860 870 880 890 900
MVSTSSLEEL HRTALSWLDQ HCSLPILRPM VLSTLRQLST STSILTDPAQ
910
LPEQASKAVT RIGKRLGES
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF5H0V4 | F5H0V4_HUMAN | MLX-interacting protein | MLXIP | 308 | Annotation score: | ||
H0YGR9 | H0YGR9_HUMAN | MLX-interacting protein | MLXIP | 171 | Annotation score: | ||
F5H321 | F5H321_HUMAN | MLX-interacting protein | MLXIP | 73 | Annotation score: |
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_059344 | 396 | E → G2 PublicationsCorresponds to variant dbSNP:rs7978353Ensembl. | 1 | |
Natural variantiVAR_059345 | 539 | V → L. Corresponds to variant dbSNP:rs34702867Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_052499 | 1 – 393 | Missing in isoform 2, isoform 4 and isoform 5. 3 PublicationsAdd BLAST | 393 | |
Alternative sequenceiVSP_052500 | 426 – 443 | Missing in isoform 5. 1 PublicationAdd BLAST | 18 | |
Alternative sequenceiVSP_052501 | 578 – 628 | AAFSG…EFHSS → GEPGGETQCGAPPDPEGCFP IPKAFKLVTTTTTLVCTCMR THIHLNETKVS in isoform 3 and isoform 5. 1 PublicationAdd BLAST | 51 | |
Alternative sequenceiVSP_052502 | 629 – 919 | Missing in isoform 3 and isoform 5. 1 PublicationAdd BLAST | 291 | |
Alternative sequenceiVSP_052503 | 770 – 772 | QQE → LLS in isoform 4. 1 Publication | 3 | |
Alternative sequenceiVSP_052504 | 773 – 919 | Missing in isoform 4. 1 PublicationAdd BLAST | 147 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF312918 mRNA Translation: AAG34121.1 AF245480 mRNA Translation: AAL55689.1 AF245481 mRNA Translation: AAL55690.1 AB020674 mRNA Translation: BAA74890.2 Different initiation. BC017656 mRNA Translation: AAH17656.1 BC028309 mRNA Translation: AAH28309.1 BC039704 mRNA Translation: AAH39704.1 BC151841 mRNA Translation: AAI51842.1 |
CCDSi | CCDS73540.1 [Q9HAP2-1] |
RefSeqi | NP_055753.3, NM_014938.5 [Q9HAP2-1] |
Genome annotation databases
Ensembli | ENST00000319080.12; ENSP00000312834.6; ENSG00000175727.14 ENST00000377037.3; ENSP00000366236.3; ENSG00000175727.14 [Q9HAP2-5] ENST00000538698.5; ENSP00000440769.1; ENSG00000175727.14 [Q9HAP2-2] ENST00000625732.3; ENSP00000486569.2; ENSG00000281178.4 ENST00000629738.4; ENSP00000487003.1; ENSG00000281178.4 |
GeneIDi | 22877 |
KEGGi | hsa:22877 |
MANE-Selecti | ENST00000319080.12; ENSP00000312834.6; NM_014938.6; NP_055753.3 |
UCSCi | uc001ubq.4, human [Q9HAP2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF312918 mRNA Translation: AAG34121.1 AF245480 mRNA Translation: AAL55689.1 AF245481 mRNA Translation: AAL55690.1 AB020674 mRNA Translation: BAA74890.2 Different initiation. BC017656 mRNA Translation: AAH17656.1 BC028309 mRNA Translation: AAH28309.1 BC039704 mRNA Translation: AAH39704.1 BC151841 mRNA Translation: AAI51842.1 |
CCDSi | CCDS73540.1 [Q9HAP2-1] |
RefSeqi | NP_055753.3, NM_014938.5 [Q9HAP2-1] |
3D structure databases
AlphaFoldDBi | Q9HAP2 |
SMRi | Q9HAP2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 116544, 15 interactors |
IntActi | Q9HAP2, 10 interactors |
STRINGi | 9606.ENSP00000312834 |
PTM databases
GlyGeni | Q9HAP2, 8 sites, 2 O-linked glycans (8 sites) |
iPTMneti | Q9HAP2 |
PhosphoSitePlusi | Q9HAP2 |
Genetic variation databases
BioMutai | MLXIP |
DMDMi | 156632588 |
Proteomic databases
EPDi | Q9HAP2 |
jPOSTi | Q9HAP2 |
MassIVEi | Q9HAP2 |
MaxQBi | Q9HAP2 |
PaxDbi | Q9HAP2 |
PeptideAtlasi | Q9HAP2 |
PRIDEi | Q9HAP2 |
ProteomicsDBi | 81411 [Q9HAP2-1] 81412 [Q9HAP2-2] 81413 [Q9HAP2-3] 81414 [Q9HAP2-4] 81415 [Q9HAP2-5] |
Protocols and materials databases
Antibodypediai | 9815, 152 antibodies from 24 providers |
DNASUi | 22877 |
Genome annotation databases
Ensembli | ENST00000319080.12; ENSP00000312834.6; ENSG00000175727.14 ENST00000377037.3; ENSP00000366236.3; ENSG00000175727.14 [Q9HAP2-5] ENST00000538698.5; ENSP00000440769.1; ENSG00000175727.14 [Q9HAP2-2] ENST00000625732.3; ENSP00000486569.2; ENSG00000281178.4 ENST00000629738.4; ENSP00000487003.1; ENSG00000281178.4 |
GeneIDi | 22877 |
KEGGi | hsa:22877 |
MANE-Selecti | ENST00000319080.12; ENSP00000312834.6; NM_014938.6; NP_055753.3 |
UCSCi | uc001ubq.4, human [Q9HAP2-1] |
Organism-specific databases
CTDi | 22877 |
DisGeNETi | 22877 |
GeneCardsi | MLXIP |
HGNCi | HGNC:17055, MLXIP |
HPAi | ENSG00000175727, Tissue enhanced (skeletal) |
MIMi | 608090, gene |
neXtProti | NX_Q9HAP2 |
OpenTargetsi | ENSG00000175727 |
PharmGKBi | PA128394590 |
VEuPathDBi | HostDB:ENSG00000175727 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3582, Eukaryota |
GeneTreei | ENSGT00940000158691 |
HOGENOMi | CLU_007471_1_0_1 |
InParanoidi | Q9HAP2 |
OMAi | QTCQTYQ |
OrthoDBi | 388166at2759 |
PhylomeDBi | Q9HAP2 |
TreeFami | TF324749 |
Enzyme and pathway databases
PathwayCommonsi | Q9HAP2 |
SignaLinki | Q9HAP2 |
Miscellaneous databases
BioGRID-ORCSi | 22877, 28 hits in 343 CRISPR screens |
ChiTaRSi | MLXIP, human |
GenomeRNAii | 22877 |
Pharosi | Q9HAP2, Tbio |
PROi | PR:Q9HAP2 |
RNActi | Q9HAP2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000175727, Expressed in caecum and 234 other tissues |
ExpressionAtlasi | Q9HAP2, baseline and differential |
Genevisiblei | Q9HAP2, HS |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR032648, MLXIP |
PANTHERi | PTHR15741:SF23, PTHR15741:SF23, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MLXIP_HUMAN | |
Accessioni | Q9HAP2Primary (citable) accession number: Q9HAP2 Secondary accession number(s): A7MBN0 Q8WYA5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 21, 2007 |
Last sequence update: | August 21, 2007 | |
Last modified: | May 25, 2022 | |
This is version 150 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot