UniProtKB - Q9H9P5 (UNKL_HUMAN)
Putative E3 ubiquitin-protein ligase UNKL
UNKL
Functioni
May participate in a protein complex showing an E3 ligase activity regulated by RAC1. Ubiquitination is directed towards itself and possibly other substrates, such as SMARCD2/BAF60b. Intrinsic E3 ligase activity has not been proven.
1 PublicationCaution
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 75 – 104 | C3H1-type 1PROSITE-ProRule annotationAdd BLAST | 30 | |
Zinc fingeri | 115 – 145 | C3H1-type 2PROSITE-ProRule annotationAdd BLAST | 31 | |
Zinc fingeri | 243 – 277 | C3H1-type 3PROSITE-ProRule annotationAdd BLAST | 35 | |
Zinc fingeri | 283 – 310 | C3H1-type 4PROSITE-ProRule annotationAdd BLAST | 28 | |
Zinc fingeri | 639 – 674 | RING-typePROSITE-ProRule annotationAdd BLAST | 36 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- transferase activity Source: UniProtKB-KW
GO - Biological processi
- protein ubiquitination Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9H9P5 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | Q9H9P5 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Putative E3 ubiquitin-protein ligase UNKL (EC:2.3.2.-)Alternative name(s): RING finger protein unkempt-like Zinc finger CCCH domain-containing protein 5-like |
Gene namesi | Name:UNKL Synonyms:C16orf28, ZC3H5L, ZC3HDC5L |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:14184, UNKL |
MIMi | 617463, gene |
neXtProti | NX_Q9H9P5 |
VEuPathDBi | HostDB:ENSG00000059145 |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 639 | C → A: No effects on the interaction with RAC1 or SMARCD2; when associated with A-670. 1 Publication | 1 | |
Mutagenesisi | 670 | C → A: No effects on the interaction with RAC1 or SMARCD2; when associated with A-639. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 64718 |
OpenTargetsi | ENSG00000059145 |
PharmGKBi | PA37855 |
Miscellaneous databases
Pharosi | Q9H9P5, Tdark |
Genetic variation databases
BioMutai | UNKL |
DMDMi | 300669704 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000278667 | 1 – 680 | Putative E3 ubiquitin-protein ligase UNKLAdd BLAST | 680 |
Post-translational modificationi
Keywords - PTMi
Ubl conjugationProteomic databases
EPDi | Q9H9P5 |
jPOSTi | Q9H9P5 |
MassIVEi | Q9H9P5 |
MaxQBi | Q9H9P5 |
PaxDbi | Q9H9P5 |
PeptideAtlasi | Q9H9P5 |
PRIDEi | Q9H9P5 |
ProteomicsDBi | 81339 [Q9H9P5-4] 81340 [Q9H9P5-1] 81341 [Q9H9P5-2] 81342 [Q9H9P5-3] 81343 [Q9H9P5-5] 81344 [Q9H9P5-6] |
PTM databases
iPTMneti | Q9H9P5 |
PhosphoSitePlusi | Q9H9P5 |
Expressioni
Gene expression databases
Bgeei | ENSG00000059145, Expressed in secondary oocyte and 215 other tissues |
ExpressionAtlasi | Q9H9P5, baseline and differential |
Genevisiblei | Q9H9P5, HS |
Organism-specific databases
HPAi | ENSG00000059145, Low tissue specificity |
Interactioni
Subunit structurei
Isoform 4 (C-terminal) interacts with the GTP-bound form of RAC1. Isoform 4 (C-terminal) interacts with SMARCD2/BAF60b.
1 PublicationBinary interactionsi
Q9H9P5
With | #Exp. | IntAct |
---|---|---|
RAC1 [P63000] | 2 | EBI-7797561,EBI-413628 |
SMARCD2 [Q92925] | 2 | EBI-7797561,EBI-358441 |
Isoform 5 [Q9H9P5-5]
Protein-protein interaction databases
BioGRIDi | 122243, 51 interactors |
IntActi | Q9H9P5, 34 interactors |
MINTi | Q9H9P5 |
STRINGi | 9606.ENSP00000301712 |
Miscellaneous databases
RNActi | Q9H9P5, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 22 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 326 – 358 | DisorderedSequence analysisAdd BLAST | 33 | |
Regioni | 375 – 400 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 442 – 520 | DisorderedSequence analysisAdd BLAST | 79 | |
Regioni | 545 – 566 | DisorderedSequence analysisAdd BLAST | 22 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 563 – 619 | Sequence analysisAdd BLAST | 57 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 375 – 396 | Polar residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 470 – 494 | Polar residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 495 – 509 | Pro residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 545 – 564 | Polar residuesSequence analysisAdd BLAST | 20 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 75 – 104 | C3H1-type 1PROSITE-ProRule annotationAdd BLAST | 30 | |
Zinc fingeri | 115 – 145 | C3H1-type 2PROSITE-ProRule annotationAdd BLAST | 31 | |
Zinc fingeri | 243 – 277 | C3H1-type 3PROSITE-ProRule annotationAdd BLAST | 35 | |
Zinc fingeri | 283 – 310 | C3H1-type 4PROSITE-ProRule annotationAdd BLAST | 28 | |
Zinc fingeri | 639 – 674 | RING-typePROSITE-ProRule annotationAdd BLAST | 36 |
Keywords - Domaini
Coiled coil, Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1595, Eukaryota |
GeneTreei | ENSGT00940000158822 |
HOGENOMi | CLU_1363135_0_0_1 |
InParanoidi | Q9H9P5 |
OrthoDBi | 720138at2759 |
PhylomeDBi | Q9H9P5 |
TreeFami | TF314982 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR045234, Unkempt-like IPR040594, Unkempt_Znf IPR000571, Znf_CCCH IPR036855, Znf_CCCH_sf IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR14493, PTHR14493, 2 hits |
Pfami | View protein in Pfam PF00642, zf-CCCH, 1 hit PF18384, zf_CCCH_5, 1 hit |
SMARTi | View protein in SMART SM00356, ZnF_C3H1, 4 hits |
SUPFAMi | SSF90229, SSF90229, 1 hit |
PROSITEi | View protein in PROSITE PS50103, ZF_C3H1, 4 hits PS50089, ZF_RING_2, 1 hit |
s (6+)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPSVSKAAAA ALSGSPPQTE KPTHYRYLKE FRTEQCPLFS QHKCAQHRPF
60 70 80 90 100
TCFHWHFLNQ RRRRPLRRRD GTFNYSPDVY CSKYNEATGV CPDGDECPYL
110 120 130 140 150
HRTTGDTERK YHLRYYKTGT CIHETDARGH CVKNGLHCAF AHGPLDLRPP
160 170 180 190 200
VCDVRELQAQ EALQNGQLGG GEGVPDLQPG VLASQAMIEK ILSEDPRWQD
210 220 230 240 250
ANFVLGSYKT EQCPKPPRLC RQGYACPHYH NSRDRRRNPR RFQYRSTPCP
260 270 280 290 300
SVKHGDEWGE PSRCDGGDGC QYCHSRTEQQ FHPESTKCND MRQTGYCPRG
310 320 330 340 350
PFCAFAHVEK SLGMVNEWGC HDLHLTSPSS TGSGQPGNAK RRDSPAEGGP
360 370 380 390 400
RGSEQDSKQN HLAVFAAVHP PAPSVSSSVA SSLASSAGSG SSSPTALPAP
410 420 430 440 450
PARALPLGPA SSTVEAVLGS ALDLHLSNVN IASLEKDLEE QDGHDLGAAG
460 470 480 490 500
PRSLAGSAPV AIPGSLPRAP SLHSPSSAST SPLGSLSQPL PGPVGSSAMT
510 520 530 540 550
PPQQPPPLRS EPGTLGSAAS SYSPLGLNGV PGSIWDFVSG SFSPSPSPIL
560 570 580 590 600
SAGPPSSSSA SPNGAELARV RRQLDEAKRK IRQWEESWQQ VKQVCDAWQR
610 620 630 640 650
EAQEAKERAR VADSDRQLAL QKKEEVEAQV IFQLRAKQCV ACRERAHGAV
660 670 680
LRPCQHHILC EPCAATAPEC PYCKGQPLQW
The sequence of this isoform differs from the canonical sequence as follows:
96-96: E → ESLLGVLYPL...GVWWTLLGFR
199-199: Q → QALLPVALLRHRVAHFS
284-284: E → EGPVGSPVSG...PHVCVFPIYK
359-359: Q → QGVRAHGVYVFEQ
419-554: GSALDLHLSN...SPSPILSAGP → ALEPTPSSPT...HRQDTRPVPS
555-680: Missing.
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PDK2 | E9PDK2_HUMAN | Putative E3 ubiquitin-protein ligas... | UNKL | 683 | Annotation score: | ||
A0A0A0MRZ1 | A0A0A0MRZ1_HUMAN | Putative E3 ubiquitin-protein ligas... | UNKL | 733 | Annotation score: | ||
A0A0A0MSC7 | A0A0A0MSC7_HUMAN | Putative E3 ubiquitin-protein ligas... | UNKL | 114 | Annotation score: | ||
D6RA68 | D6RA68_HUMAN | Putative E3 ubiquitin-protein ligas... | UNKL | 76 | Annotation score: | ||
E2QRG9 | E2QRG9_HUMAN | Putative E3 ubiquitin-protein ligas... | UNKL | 116 | Annotation score: | ||
A0A0A0MSF0 | A0A0A0MSF0_HUMAN | Putative E3 ubiquitin-protein ligas... | UNKL | 52 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 541 | S → R in CAQ16184 (PubMed:20148946).Curated | 1 | |
Sequence conflicti | 549 | I → V in BAB14178 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_039432 | 1 – 501 | Missing in isoform 3. 1 PublicationAdd BLAST | 501 | |
Alternative sequenceiVSP_039433 | 1 – 498 | Missing in isoform 1 and isoform 2. 1 PublicationAdd BLAST | 498 | |
Alternative sequenceiVSP_039434 | 96 | E → ESLLGVLYPLWAPPPVPAPV GAPTVHPFLARWFSHVPWEP AGSADPRASSTPAGGTTWAT GLLGDFDACGSQSRECVKGV WWTLLGFR in isoform 6. Curated | 1 | |
Alternative sequenceiVSP_039435 | 199 | Q → QALLPVALLRHRVAHFS in isoform 6. Curated | 1 | |
Alternative sequenceiVSP_039436 | 245 – 277 | RSTPC…CHSRT → SWQLGRRVLRLSPRANNPRV ALPRVHTGPSSTA in isoform 5. 1 PublicationAdd BLAST | 33 | |
Alternative sequenceiVSP_039437 | 278 – 680 | Missing in isoform 5. 1 PublicationAdd BLAST | 403 | |
Alternative sequenceiVSP_039438 | 284 | E → EGPVGSPVSGAFSVFSHAGL VADPSLWYPAEWSWHHVGHS RTINPEGDKPSRLGPAPENI KRGNDFACDGRADAAGMAPH VCVFPIYK in isoform 6. Curated | 1 | |
Alternative sequenceiVSP_039439 | 359 | Q → QGVRAHGVYVFEQ in isoform 6. Curated | 1 | |
Alternative sequenceiVSP_039440 | 419 – 554 | GSALD…LSAGP → ALEPTPSSPTSSAVQGVAGE LGMGSGGQGCWSHVVGALAA VPAWTHSGDLEWDPSTIRTR VNVGGGPAARPAMTLAREPQ LLVVGGCPTRNCSGPAASPQ QLLDDAGQGGRGERDSSQRP LRPQTTHRQDTRPVPS in isoform 6. CuratedAdd BLAST | 136 | |
Alternative sequenceiVSP_039441 | 502 – 525 | PQQPP…SYSPL → MTCCSQVPPRRRPSLALSPR LDCN in isoform 3. 1 PublicationAdd BLAST | 24 | |
Alternative sequenceiVSP_039442 | 555 – 680 | Missing in isoform 6. CuratedAdd BLAST | 126 | |
Alternative sequenceiVSP_039443 | 630 | V → VKQLQEELEGLGVASTLPGL RGCGDIGTIPLPKLHSLQSQ LRLDLEAVDGV in isoform 1 and isoform 3. 2 Publications | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AM944365 mRNA Translation: CAQ16184.1 AK022685 mRNA Translation: BAB14178.1 AK027013 mRNA Translation: BAB15626.1 AE006467 Genomic DNA Translation: AAK61278.1 AE006467 Genomic DNA Translation: AAK61279.1 AL031709 Genomic DNA No translation available. AL031721 Genomic DNA No translation available. AL032819 Genomic DNA No translation available. CH471112 Genomic DNA Translation: EAW85659.1 CH471112 Genomic DNA Translation: EAW85660.1 CH471112 Genomic DNA Translation: EAW85662.1 BC000150 mRNA Translation: AAH00150.1 BC011924 mRNA Translation: AAH11924.1 |
CCDSi | CCDS32359.1 [Q9H9P5-5] CCDS53980.1 [Q9H9P5-2] CCDS61787.1 [Q9H9P5-3] |
PIRi | T45063 |
RefSeqi | NP_001032202.1, NM_001037125.3 [Q9H9P5-5] NP_001180317.2, NM_001193388.3 NP_001180318.1, NM_001193389.1 [Q9H9P5-2] NP_001263343.1, NM_001276414.1 [Q9H9P5-3] XP_016879057.1, XM_017023568.1 XP_016879058.1, XM_017023569.1 [Q9H9P5-1] |
Genome annotation databases
Ensembli | ENST00000248104; ENSP00000248104; ENSG00000059145 [Q9H9P5-3] ENST00000301712; ENSP00000301712; ENSG00000059145 [Q9H9P5-5] ENST00000397464; ENSP00000380606; ENSG00000059145 [Q9H9P5-2] ENST00000674376; ENSP00000501444; ENSG00000059145 [Q9H9P5-2] |
GeneIDi | 64718 |
KEGGi | hsa:64718 |
UCSCi | uc002clo.3, human [Q9H9P5-4] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AM944365 mRNA Translation: CAQ16184.1 AK022685 mRNA Translation: BAB14178.1 AK027013 mRNA Translation: BAB15626.1 AE006467 Genomic DNA Translation: AAK61278.1 AE006467 Genomic DNA Translation: AAK61279.1 AL031709 Genomic DNA No translation available. AL031721 Genomic DNA No translation available. AL032819 Genomic DNA No translation available. CH471112 Genomic DNA Translation: EAW85659.1 CH471112 Genomic DNA Translation: EAW85660.1 CH471112 Genomic DNA Translation: EAW85662.1 BC000150 mRNA Translation: AAH00150.1 BC011924 mRNA Translation: AAH11924.1 |
CCDSi | CCDS32359.1 [Q9H9P5-5] CCDS53980.1 [Q9H9P5-2] CCDS61787.1 [Q9H9P5-3] |
PIRi | T45063 |
RefSeqi | NP_001032202.1, NM_001037125.3 [Q9H9P5-5] NP_001180317.2, NM_001193388.3 NP_001180318.1, NM_001193389.1 [Q9H9P5-2] NP_001263343.1, NM_001276414.1 [Q9H9P5-3] XP_016879057.1, XM_017023568.1 XP_016879058.1, XM_017023569.1 [Q9H9P5-1] |
3D structure databases
SMRi | Q9H9P5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 122243, 51 interactors |
IntActi | Q9H9P5, 34 interactors |
MINTi | Q9H9P5 |
STRINGi | 9606.ENSP00000301712 |
PTM databases
iPTMneti | Q9H9P5 |
PhosphoSitePlusi | Q9H9P5 |
Genetic variation databases
BioMutai | UNKL |
DMDMi | 300669704 |
Proteomic databases
EPDi | Q9H9P5 |
jPOSTi | Q9H9P5 |
MassIVEi | Q9H9P5 |
MaxQBi | Q9H9P5 |
PaxDbi | Q9H9P5 |
PeptideAtlasi | Q9H9P5 |
PRIDEi | Q9H9P5 |
ProteomicsDBi | 81339 [Q9H9P5-4] 81340 [Q9H9P5-1] 81341 [Q9H9P5-2] 81342 [Q9H9P5-3] 81343 [Q9H9P5-5] 81344 [Q9H9P5-6] |
Protocols and materials databases
Antibodypediai | 34818, 111 antibodies from 19 providers |
DNASUi | 64718 |
Genome annotation databases
Ensembli | ENST00000248104; ENSP00000248104; ENSG00000059145 [Q9H9P5-3] ENST00000301712; ENSP00000301712; ENSG00000059145 [Q9H9P5-5] ENST00000397464; ENSP00000380606; ENSG00000059145 [Q9H9P5-2] ENST00000674376; ENSP00000501444; ENSG00000059145 [Q9H9P5-2] |
GeneIDi | 64718 |
KEGGi | hsa:64718 |
UCSCi | uc002clo.3, human [Q9H9P5-4] |
Organism-specific databases
CTDi | 64718 |
DisGeNETi | 64718 |
GeneCardsi | UNKL |
HGNCi | HGNC:14184, UNKL |
HPAi | ENSG00000059145, Low tissue specificity |
MIMi | 617463, gene |
neXtProti | NX_Q9H9P5 |
OpenTargetsi | ENSG00000059145 |
PharmGKBi | PA37855 |
VEuPathDBi | HostDB:ENSG00000059145 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1595, Eukaryota |
GeneTreei | ENSGT00940000158822 |
HOGENOMi | CLU_1363135_0_0_1 |
InParanoidi | Q9H9P5 |
OrthoDBi | 720138at2759 |
PhylomeDBi | Q9H9P5 |
TreeFami | TF314982 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q9H9P5 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | Q9H9P5 |
Miscellaneous databases
BioGRID-ORCSi | 64718, 7 hits in 1045 CRISPR screens |
ChiTaRSi | UNKL, human |
GeneWikii | UNKL |
GenomeRNAii | 64718 |
Pharosi | Q9H9P5, Tdark |
PROi | PR:Q9H9P5 |
RNActi | Q9H9P5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000059145, Expressed in secondary oocyte and 215 other tissues |
ExpressionAtlasi | Q9H9P5, baseline and differential |
Genevisiblei | Q9H9P5, HS |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR045234, Unkempt-like IPR040594, Unkempt_Znf IPR000571, Znf_CCCH IPR036855, Znf_CCCH_sf IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR14493, PTHR14493, 2 hits |
Pfami | View protein in Pfam PF00642, zf-CCCH, 1 hit PF18384, zf_CCCH_5, 1 hit |
SMARTi | View protein in SMART SM00356, ZnF_C3H1, 4 hits |
SUPFAMi | SSF90229, SSF90229, 1 hit |
PROSITEi | View protein in PROSITE PS50103, ZF_C3H1, 4 hits PS50089, ZF_RING_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | UNKL_HUMAN | |
Accessioni | Q9H9P5Primary (citable) accession number: Q9H9P5 Secondary accession number(s): B0QYN6 Q9UJJ8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 6, 2007 |
Last sequence update: | July 13, 2010 | |
Last modified: | February 23, 2022 | |
This is version 159 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families