UniProtKB - Q9H981 (ARP8_HUMAN)
Actin-related protein 8
ACTR8
Functioni
Plays an important role in the functional organization of mitotic chromosomes. Exhibits low basal ATPase activity, and unable to polymerize.
Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. Required for the recruitment of INO80 (and probably the INO80 complex) to sites of DNA damage. Strongly prefer nucleosomes and H3-H4 tetramers over H2A-H2B dimers, suggesting it may act as a nucleosome recognition module within the complex.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 55 | ATP | 1 | |
Binding sitei | 56 | ATP | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 283 – 286 | ATP | 4 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- chromatin remodeling Source: ComplexPortal
- DNA recombination Source: UniProtKB-KW
- double-strand break repair Source: GO_Central
- nucleosome mobilization Source: ComplexPortal
- positive regulation of DNA repair Source: Ensembl
- positive regulation of telomere maintenance in response to DNA damage Source: Ensembl
- positive regulation of transcription, DNA-templated Source: ComplexPortal
- regulation of cell cycle Source: ComplexPortal
- regulation of chromosome organization Source: ComplexPortal
- regulation of DNA replication Source: ComplexPortal
- regulation of DNA strand elongation Source: ComplexPortal
- regulation of embryonic development Source: Ensembl
- regulation of transcription, DNA-templated Source: GO_Central
- telomere maintenance Source: Ensembl
Keywordsi
Biological process | Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, Mitosis, Transcription, Transcription regulation |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9H981 |
Reactomei | R-HSA-5689603, UCH proteinases R-HSA-5696394, DNA Damage Recognition in GG-NER |
SignaLinki | Q9H981 |
Names & Taxonomyi
Protein namesi | Recommended name: Actin-related protein 8Short name: hArp8 Alternative name(s): INO80 complex subunit N |
Gene namesi | Name:ACTR8 Synonyms:ARP8, INO80N |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:14672, ACTR8 |
neXtProti | NX_Q9H981 |
VEuPathDBi | HostDB:ENSG00000113812 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Other locations
- Chromosome 1 Publication
Note: Specifically localizes to mitotic chromosomes.
Cytoskeleton
- centrosome Source: HPA
Nucleus
- Ino80 complex Source: UniProtKB
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Keywords - Cellular componenti
Chromosome, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 93973 |
OpenTargetsi | ENSG00000113812 |
PharmGKBi | PA24491 |
Miscellaneous databases
Pharosi | Q9H981, Tbio |
Genetic variation databases
BioMutai | ACTR8 |
DMDMi | 116241257 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000089123 | 1 – 624 | Actin-related protein 8Add BLAST | 624 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 132 | PhosphoserineCombined sources | 1 | |
Modified residuei | 412 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9H981 |
jPOSTi | Q9H981 |
MassIVEi | Q9H981 |
MaxQBi | Q9H981 |
PaxDbi | Q9H981 |
PeptideAtlasi | Q9H981 |
PRIDEi | Q9H981 |
ProteomicsDBi | 81292 [Q9H981-1] 81293 [Q9H981-2] 81294 [Q9H981-3] |
PTM databases
iPTMneti | Q9H981 |
PhosphoSitePlusi | Q9H981 |
Expressioni
Gene expression databases
Bgeei | ENSG00000113812, Expressed in testis and 208 other tissues |
ExpressionAtlasi | Q9H981, baseline and differential |
Genevisiblei | Q9H981, HS |
Organism-specific databases
HPAi | ENSG00000113812, Low tissue specificity |
Interactioni
Subunit structurei
Component of the chromatin remodeling INO80 complex; specifically part of a complex module associated with the DBINO domain of INO80.
Interacts with ACTR5; the interaction is observed in asynchronous (interphase) cells but not in metaphase-arrested cells indicative for a possible dissociation of the INO80 complex in mitotic cells. Exists as monomers and dimers, but the dimer is most probably the biologically relevant form required for stable interactions with histones that exploits the twofold symmetry of the nucleosome core.
5 PublicationsBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 125062, 59 interactors |
ComplexPortali | CPX-846, INO80 chromatin remodeling complex |
CORUMi | Q9H981 |
IntActi | Q9H981, 23 interactors |
MINTi | Q9H981 |
STRINGi | 9606.ENSP00000336842 |
Miscellaneous databases
RNActi | Q9H981, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9H981 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 29 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 430 – 462 | DisorderedSequence analysisAdd BLAST | 33 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 27 | Basic and acidic residuesSequence analysisAdd BLAST | 27 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0797, Eukaryota |
GeneTreei | ENSGT00390000001763 |
HOGENOMi | CLU_006974_1_0_1 |
InParanoidi | Q9H981 |
OMAi | EDCFDAE |
OrthoDBi | 1258783at2759 |
PhylomeDBi | Q9H981 |
TreeFami | TF324575 |
Family and domain databases
DisProti | DP00873 |
InterProi | View protein in InterPro IPR004000, Actin IPR027668, Arp8/plant_Arp9 IPR043129, ATPase_NBD |
PANTHERi | PTHR11937, PTHR11937, 1 hit PTHR11937:SF13, PTHR11937:SF13, 1 hit |
Pfami | View protein in Pfam PF00022, Actin, 1 hit |
SMARTi | View protein in SMART SM00268, ACTIN, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTQAEKGDTE NGKEKGGEKE KEQRGVKRPI VPALVPESLQ EQIQSNFIIV
60 70 80 90 100
IHPGSTTLRI GRATDTLPAS IPHVIARRHK QQGQPLYKDS WLLREGLNKP
110 120 130 140 150
ESNEQRQNGL KMVDQAIWSK KMSNGTRRIP VSPEQARSYN KQMRPAILDH
160 170 180 190 200
CSGNKWTNTS HHPEYLVGEE ALYVNPLDCY NIHWPIRRGQ LNIHPGPGGS
210 220 230 240 250
LTAVLADIEV IWSHAIQKYL EIPLKDLKYY RCILLIPDIY NKQHVKELVN
260 270 280 290 300
MILMKMGFSG IVVHQESVCA TYGSGLSSTC IVDVGDQKTS VCCVEDGVSH
310 320 330 340 350
RNTRLCLAYG GSDVSRCFYW LMQRAGFPYR ECQLTNKMDC LLLQHLKETF
360 370 380 390 400
CHLDQDISGL QDHEFQIRHP DSPALLYQFR LGDEKLQAPM ALFYPATFGI
410 420 430 440 450
VGQKMTTLQH RSQGDPEDPH DEHYLLATQS KQEQSAKATA DRKSASKPIG
460 470 480 490 500
FEGDLRGQSS DLPERLHSQE VDLGSAQGDG LMAGNDSEEA LTALMSRKTA
510 520 530 540 550
ISLFEGKALG LDKAILHSID CCSSDDTKKK MYSSILVVGG GLMFHKAQEF
560 570 580 590 600
LQHRILNKMP PSFRRIIENV DVITRPKDMD PRLIAWKGGA VLACLDTTQE
610 620
LWIYQREWQR FGVRMLRERA AFVW
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0Y849 | H0Y849_HUMAN | Actin-related protein 8 | ACTR8 | 378 | Annotation score: | ||
C9J7L6 | C9J7L6_HUMAN | Actin-related protein 8 | ACTR8 | 102 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 596 | D → G in BAB14352 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_028033 | 56 | T → I. Corresponds to variant dbSNP:rs3733082Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_040506 | 1 – 250 | Missing in isoform 3. 1 PublicationAdd BLAST | 250 | |
Alternative sequenceiVSP_040507 | 1 – 111 | Missing in isoform 2. 1 PublicationAdd BLAST | 111 | |
Alternative sequenceiVSP_040508 | 305 – 354 | LCLAY…FCHLD → IFSWN in isoform 3. 1 PublicationAdd BLAST | 50 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK022996 mRNA Translation: BAB14352.1 AK094507 mRNA Translation: BAG52879.1 AK026232 mRNA Translation: BAB15402.1 Different initiation. AC012467 Genomic DNA No translation available. BC032744 mRNA Translation: AAH32744.1 |
CCDSi | CCDS2875.1 [Q9H981-1] |
RefSeqi | NP_075050.3, NM_022899.4 [Q9H981-1] XP_005265644.1, XM_005265587.4 [Q9H981-1] XP_011532551.1, XM_011534249.2 [Q9H981-2] |
Genome annotation databases
Ensembli | ENST00000335754; ENSP00000336842; ENSG00000113812 ENST00000482349; ENSP00000419429; ENSG00000113812 [Q9H981-2] |
GeneIDi | 93973 |
KEGGi | hsa:93973 |
MANE-Selecti | ENST00000335754.8; ENSP00000336842.3; NM_022899.5; NP_075050.3 |
UCSCi | uc003dhc.5, human [Q9H981-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK022996 mRNA Translation: BAB14352.1 AK094507 mRNA Translation: BAG52879.1 AK026232 mRNA Translation: BAB15402.1 Different initiation. AC012467 Genomic DNA No translation available. BC032744 mRNA Translation: AAH32744.1 |
CCDSi | CCDS2875.1 [Q9H981-1] |
RefSeqi | NP_075050.3, NM_022899.4 [Q9H981-1] XP_005265644.1, XM_005265587.4 [Q9H981-1] XP_011532551.1, XM_011534249.2 [Q9H981-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4FO0 | X-ray | 2.60 | A | 34-624 | [»] | |
SMRi | Q9H981 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 125062, 59 interactors |
ComplexPortali | CPX-846, INO80 chromatin remodeling complex |
CORUMi | Q9H981 |
IntActi | Q9H981, 23 interactors |
MINTi | Q9H981 |
STRINGi | 9606.ENSP00000336842 |
PTM databases
iPTMneti | Q9H981 |
PhosphoSitePlusi | Q9H981 |
Genetic variation databases
BioMutai | ACTR8 |
DMDMi | 116241257 |
Proteomic databases
EPDi | Q9H981 |
jPOSTi | Q9H981 |
MassIVEi | Q9H981 |
MaxQBi | Q9H981 |
PaxDbi | Q9H981 |
PeptideAtlasi | Q9H981 |
PRIDEi | Q9H981 |
ProteomicsDBi | 81292 [Q9H981-1] 81293 [Q9H981-2] 81294 [Q9H981-3] |
Protocols and materials databases
Antibodypediai | 31436, 52 antibodies from 14 providers |
DNASUi | 93973 |
Genome annotation databases
Ensembli | ENST00000335754; ENSP00000336842; ENSG00000113812 ENST00000482349; ENSP00000419429; ENSG00000113812 [Q9H981-2] |
GeneIDi | 93973 |
KEGGi | hsa:93973 |
MANE-Selecti | ENST00000335754.8; ENSP00000336842.3; NM_022899.5; NP_075050.3 |
UCSCi | uc003dhc.5, human [Q9H981-1] |
Organism-specific databases
CTDi | 93973 |
DisGeNETi | 93973 |
GeneCardsi | ACTR8 |
HGNCi | HGNC:14672, ACTR8 |
HPAi | ENSG00000113812, Low tissue specificity |
neXtProti | NX_Q9H981 |
OpenTargetsi | ENSG00000113812 |
PharmGKBi | PA24491 |
VEuPathDBi | HostDB:ENSG00000113812 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0797, Eukaryota |
GeneTreei | ENSGT00390000001763 |
HOGENOMi | CLU_006974_1_0_1 |
InParanoidi | Q9H981 |
OMAi | EDCFDAE |
OrthoDBi | 1258783at2759 |
PhylomeDBi | Q9H981 |
TreeFami | TF324575 |
Enzyme and pathway databases
PathwayCommonsi | Q9H981 |
Reactomei | R-HSA-5689603, UCH proteinases R-HSA-5696394, DNA Damage Recognition in GG-NER |
SignaLinki | Q9H981 |
Miscellaneous databases
BioGRID-ORCSi | 93973, 498 hits in 1053 CRISPR screens |
ChiTaRSi | ACTR8, human |
GenomeRNAii | 93973 |
Pharosi | Q9H981, Tbio |
PROi | PR:Q9H981 |
RNActi | Q9H981, protein |
Gene expression databases
Bgeei | ENSG00000113812, Expressed in testis and 208 other tissues |
ExpressionAtlasi | Q9H981, baseline and differential |
Genevisiblei | Q9H981, HS |
Family and domain databases
DisProti | DP00873 |
InterProi | View protein in InterPro IPR004000, Actin IPR027668, Arp8/plant_Arp9 IPR043129, ATPase_NBD |
PANTHERi | PTHR11937, PTHR11937, 1 hit PTHR11937:SF13, PTHR11937:SF13, 1 hit |
Pfami | View protein in Pfam PF00022, Actin, 1 hit |
SMARTi | View protein in SMART SM00268, ACTIN, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | ARP8_HUMAN | |
Accessioni | Q9H981Primary (citable) accession number: Q9H981 Secondary accession number(s): B3KSW7, Q8N566, Q9H663 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 27, 2003 |
Last sequence update: | October 17, 2006 | |
Last modified: | February 23, 2022 | |
This is version 162 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families