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Protein

Protein zwilch homolog

Gene

ZWILCH

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed:15824131).1 Publication

Miscellaneous

ZWILCH gene is deleted in a patient suffering from colorectal cancer with chromosomal instability.

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic cell cycle checkpoint Source: UniProtKB

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein zwilch homolog
Short name:
hZwilch
Gene namesi
Name:ZWILCH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

EuPathDBiHostDB:ENSG00000174442.11
HGNCiHGNC:25468 ZWILCH
MIMi609984 gene
neXtProtiNX_Q9H900

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore

Pathology & Biotechi

Organism-specific databases

DisGeNETi55055
OpenTargetsiENSG00000174442
PharmGKBiPA142670465

Polymorphism and mutation databases

BioMutaiZWILCH
DMDMi166228729

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003148001 – 591Protein zwilch homologAdd BLAST591

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei88PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9H900
MaxQBiQ9H900
PaxDbiQ9H900
PeptideAtlasiQ9H900
PRIDEiQ9H900
ProteomicsDBi81259
81260 [Q9H900-2]

PTM databases

iPTMnetiQ9H900
PhosphoSitePlusiQ9H900

Expressioni

Gene expression databases

BgeeiENSG00000174442 Expressed in 189 organ(s), highest expression level in sperm
CleanExiHS_ZWILCH
ExpressionAtlasiQ9H900 baseline and differential
GenevisibleiQ9H900 HS

Interactioni

Subunit structurei

Component of the RZZ complex composed of KNTC1/ROD, ZW10 and ZWILCH; in the complex interacts directly with KNTC1/ROD.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KNTC1P507482EBI-1001239,EBI-1001245

Protein-protein interaction databases

BioGridi120376, 13 interactors
DIPiDIP-36478N
IntActiQ9H900, 3 interactors
STRINGi9606.ENSP00000311429

Structurei

Secondary structure

1591
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9H900
SMRiQ9H900
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9H900

Family & Domainsi

Sequence similaritiesi

Belongs to the ZWILCH family.Curated

Phylogenomic databases

eggNOGiKOG4803 Eukaryota
ENOG410Y8MB LUCA
GeneTreeiENSGT00390000013696
HOGENOMiHOG000155813
HOVERGENiHBG108779
InParanoidiQ9H900
KOiK11579
OMAiLVSCMLF
OrthoDBiEOG091G05A6
PhylomeDBiQ9H900
TreeFamiTF324453

Family and domain databases

InterProiView protein in InterPro
IPR018630 Zwilch
PANTHERiPTHR15995 PTHR15995, 1 hit
PfamiView protein in Pfam
PF09817 Zwilch, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9H900-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MWERLNCAAE DFYSRLLQKF NEEKKGIRKD PFLYEADVQV QLISKGQPNP
60 70 80 90 100
LKNILNENDI VFIVEKVPLE KEETSHIEEL QSEETAISDF STGENVGPLA
110 120 130 140 150
LPVGKARQLI GLYTMAHNPN MTHLKINLPV TALPPLWVRC DSSDPEGTCW
160 170 180 190 200
LGAELITTNN SITGIVLYVV SCKADKNYSV NLENLKNLHK KRHHLSTVTS
210 220 230 240 250
KGFAQYELFK SSALDDTITA SQTAIALDIS WSPVDEILQI PPLSSTATLN
260 270 280 290 300
IKVESGEPRG PLNHLYRELK FLLVLADGLR TGVTEWLEPL EAKSAVELVQ
310 320 330 340 350
EFLNDLNKLD GFGDSTKKDT EVETLKHDTA AVDRSVKRLF KVRSDLDFAE
360 370 380 390 400
QLWCKMSSSV ISYQDLVKCF TLIIQSLQRG DIQPWLHSGS NSLLSKLIHQ
410 420 430 440 450
SYHGTMDTVS LSGTIPVQML LEIGLDKLKK DYISFFIGQE LASLNHLEYF
460 470 480 490 500
IAPSVDIQEQ VYRVQKLHHI LEILVSCMPF IKSQHELLFS LTQICIKYYK
510 520 530 540 550
QNPLDEQHIF QLPVRPTAVK NLYQSEKPQK WRVEIYSGQK KIKTVWQLSD
560 570 580 590
SSPIDHLNFH KPDFSELTLN GSLEERIFFT NMVTCSQVHF K
Length:591
Mass (Da):67,214
Last modified:January 15, 2008 - v2
Checksum:i522ECA229D2C0AE9
GO
Isoform 2 (identifier: Q9H900-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-114: Missing.

Show »
Length:477
Mass (Da):54,224
Checksum:i0443A2B101C275C1
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BQ07H3BQ07_HUMAN
Protein zwilch homolog
ZWILCH
98Annotation score:
H3BSG1H3BSG1_HUMAN
Protein zwilch homolog
ZWILCH
84Annotation score:
H3BPI7H3BPI7_HUMAN
Protein zwilch homolog
ZWILCH
48Annotation score:

Sequence cautioni

The sequence AK000898 differs from that shown. Reason: Frameshift at position 248.Curated
The sequence AK000898 differs from that shown. Reason: Erroneous termination at position 366. Translated as Leu.Curated
The sequence BAB55133 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti239Q → R in CAE45821 (PubMed:17974005).Curated1
Sequence conflicti537S → R in BAB14446 (PubMed:14702039).Curated1
Sequence conflicti555D → G in AK000898 (PubMed:14702039).Curated1
Sequence conflicti570N → S in AK000898 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_038055344S → G. Corresponds to variant dbSNP:rs11071896Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0303651 – 114Missing in isoform 2. 3 PublicationsAdd BLAST114

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000898 mRNA No translation available.
AK023175 mRNA Translation: BAB14446.1
AK027468 mRNA Translation: BAB55133.1 Different initiation.
AK122787 mRNA Translation: BAG53730.1
BX640701 mRNA Translation: CAE45821.1
CH471082 Genomic DNA Translation: EAW77779.1
BC036900 mRNA Translation: AAH36900.1
BC090041 mRNA Translation: AAH90041.1
CCDSiCCDS10219.1 [Q9H900-1]
CCDS73746.1 [Q9H900-2]
RefSeqiNP_001274750.1, NM_001287821.1 [Q9H900-2]
NP_001274751.1, NM_001287822.1 [Q9H900-2]
NP_001274752.1, NM_001287823.1 [Q9H900-2]
NP_060445.3, NM_017975.4 [Q9H900-1]
UniGeneiHs.21331

Genome annotation databases

EnsembliENST00000307897; ENSP00000311429; ENSG00000174442 [Q9H900-1]
ENST00000446801; ENSP00000402217; ENSG00000174442 [Q9H900-2]
ENST00000535141; ENSP00000437749; ENSG00000174442 [Q9H900-2]
ENST00000565627; ENSP00000454737; ENSG00000174442 [Q9H900-2]
ENST00000613446; ENSP00000477955; ENSG00000174442 [Q9H900-2]
GeneIDi55055
KEGGihsa:55055
UCSCiuc002aqa.5 human [Q9H900-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000898 mRNA No translation available.
AK023175 mRNA Translation: BAB14446.1
AK027468 mRNA Translation: BAB55133.1 Different initiation.
AK122787 mRNA Translation: BAG53730.1
BX640701 mRNA Translation: CAE45821.1
CH471082 Genomic DNA Translation: EAW77779.1
BC036900 mRNA Translation: AAH36900.1
BC090041 mRNA Translation: AAH90041.1
CCDSiCCDS10219.1 [Q9H900-1]
CCDS73746.1 [Q9H900-2]
RefSeqiNP_001274750.1, NM_001287821.1 [Q9H900-2]
NP_001274751.1, NM_001287822.1 [Q9H900-2]
NP_001274752.1, NM_001287823.1 [Q9H900-2]
NP_060445.3, NM_017975.4 [Q9H900-1]
UniGeneiHs.21331

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IF8X-ray2.55A1-334[»]
B335-591[»]
ProteinModelPortaliQ9H900
SMRiQ9H900
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120376, 13 interactors
DIPiDIP-36478N
IntActiQ9H900, 3 interactors
STRINGi9606.ENSP00000311429

PTM databases

iPTMnetiQ9H900
PhosphoSitePlusiQ9H900

Polymorphism and mutation databases

BioMutaiZWILCH
DMDMi166228729

Proteomic databases

EPDiQ9H900
MaxQBiQ9H900
PaxDbiQ9H900
PeptideAtlasiQ9H900
PRIDEiQ9H900
ProteomicsDBi81259
81260 [Q9H900-2]

Protocols and materials databases

DNASUi55055
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000307897; ENSP00000311429; ENSG00000174442 [Q9H900-1]
ENST00000446801; ENSP00000402217; ENSG00000174442 [Q9H900-2]
ENST00000535141; ENSP00000437749; ENSG00000174442 [Q9H900-2]
ENST00000565627; ENSP00000454737; ENSG00000174442 [Q9H900-2]
ENST00000613446; ENSP00000477955; ENSG00000174442 [Q9H900-2]
GeneIDi55055
KEGGihsa:55055
UCSCiuc002aqa.5 human [Q9H900-1]

Organism-specific databases

CTDi55055
DisGeNETi55055
EuPathDBiHostDB:ENSG00000174442.11
GeneCardsiZWILCH
H-InvDBiHIX0012366
HGNCiHGNC:25468 ZWILCH
MIMi609984 gene
neXtProtiNX_Q9H900
OpenTargetsiENSG00000174442
PharmGKBiPA142670465
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4803 Eukaryota
ENOG410Y8MB LUCA
GeneTreeiENSGT00390000013696
HOGENOMiHOG000155813
HOVERGENiHBG108779
InParanoidiQ9H900
KOiK11579
OMAiLVSCMLF
OrthoDBiEOG091G05A6
PhylomeDBiQ9H900
TreeFamiTF324453

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase

Miscellaneous databases

EvolutionaryTraceiQ9H900
GenomeRNAii55055
PROiPR:Q9H900
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000174442 Expressed in 189 organ(s), highest expression level in sperm
CleanExiHS_ZWILCH
ExpressionAtlasiQ9H900 baseline and differential
GenevisibleiQ9H900 HS

Family and domain databases

InterProiView protein in InterPro
IPR018630 Zwilch
PANTHERiPTHR15995 PTHR15995, 1 hit
PfamiView protein in Pfam
PF09817 Zwilch, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiZWILC_HUMAN
AccessioniPrimary (citable) accession number: Q9H900
Secondary accession number(s): B3KVB8
, Q6N049, Q8N404, Q96SY7, Q9NWG7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: November 7, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
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