UniProtKB - Q9H8W4 (PKHF2_HUMAN)
Protein
Pleckstrin homology domain-containing family F member 2
Gene
PLEKHF2
Organism
Homo sapiens (Human)
Status
Functioni
May play a role in early endosome fusion upstream of RAB5, hence regulating receptor trafficking and fluid-phase transport. Enhances cellular sensitivity to TNF-induced apoptosis (PubMed:18288467).3 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 152 – 212 | FYVE-typePROSITE-ProRule annotationAdd BLAST | 61 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- phosphatidylinositol binding Source: GO_Central
GO - Biological processi
- protein transport Source: UniProtKB-KW
Keywordsi
Biological process | Protein transport, Transport |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9H8W4 |
SIGNORi | Q9H8W4 |
Names & Taxonomyi
Protein namesi | Recommended name: Pleckstrin homology domain-containing family F member 2Short name: PH domain-containing family F member 2 Alternative name(s): Endoplasmic reticulum-associated apoptosis-involved protein containing PH and FYVE domains Short name: EAPF PH and FYVE domain-containing protein 2 Phafin-2 Short name: Phafin2 Zinc finger FYVE domain-containing protein 18 |
Gene namesi | Name:PLEKHF2 Synonyms:ZFYVE18 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:20757, PLEKHF2 |
MIMi | 615208, gene |
neXtProti | NX_Q9H8W4 |
VEuPathDBi | HostDB:ENSG00000175895.3 |
Subcellular locationi
Endosome
- Early endosome membrane 2 Publications; Peripheral membrane protein 2 Publications
Endoplasmic reticulum
- Endoplasmic reticulum 1 Publication
Note: Colocalizes with EEA1 and RAB5 at endosomal membrane fusion hot spots (PubMed:19995552). May translocate to the endoplasmic reticulum in the early phase of apoptosis (PubMed:18288467).2 Publications
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB-SubCell
Endosome
- early endosome membrane Source: UniProtKB-SubCell
Other locations
- transport vesicle Source: LIFEdb
Keywords - Cellular componenti
Endoplasmic reticulum, Endosome, MembranePathology & Biotechi
Organism-specific databases
OpenTargetsi | ENSG00000175895 |
PharmGKBi | PA128394715 |
Miscellaneous databases
Pharosi | Q9H8W4, Tbio |
Genetic variation databases
BioMutai | PLEKHF2 |
DMDMi | 74762744 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000251600 | 1 – 249 | Pleckstrin homology domain-containing family F member 2Add BLAST | 249 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 16 | PhosphoserineCombined sources | 1 | |
Modified residuei | 44 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 239 | PhosphoserineCombined sources | 1 | |
Modified residuei | 248 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9H8W4 |
jPOSTi | Q9H8W4 |
MassIVEi | Q9H8W4 |
MaxQBi | Q9H8W4 |
PaxDbi | Q9H8W4 |
PeptideAtlasi | Q9H8W4 |
PRIDEi | Q9H8W4 |
ProteomicsDBi | 81249 |
PTM databases
iPTMneti | Q9H8W4 |
PhosphoSitePlusi | Q9H8W4 |
Expressioni
Tissue specificityi
Expressed in placenta, ovary and small intestine, as well as in heart and pancreas. Also expressed in peripheral blood mononuclear cells and dendritic cells.1 Publication
Inductioni
Up-regulated by TNF, bacterial lipopolysaccharides (LPS) and phorbol myristate acetate (PMA) (at protein level).1 Publication
Gene expression databases
Bgeei | ENSG00000175895, Expressed in secondary oocyte and 220 other tissues |
Genevisiblei | Q9H8W4, HS |
Organism-specific databases
HPAi | ENSG00000175895, Low tissue specificity |
Interactioni
Subunit structurei
May interact with EEA1.
Binary interactionsi
Q9H8W4
Protein-protein interaction databases
BioGRIDi | 122791, 174 interactors |
IntActi | Q9H8W4, 168 interactors |
MINTi | Q9H8W4 |
STRINGi | 9606.ENSP00000322373 |
Miscellaneous databases
RNActi | Q9H8W4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 35 – 131 | PHPROSITE-ProRule annotationAdd BLAST | 97 |
Domaini
The PH and FYVE domains may be important for TNF-induced localization to the endoplasmic reticulum and for enhanced cellular sensitivity to TNF-induced apoptosis (PubMed:18288467). The FYVE domain is important for binding to the endosomal membrane.1 Publication
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 152 – 212 | FYVE-typePROSITE-ProRule annotationAdd BLAST | 61 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG1729, Eukaryota |
GeneTreei | ENSGT00940000156408 |
HOGENOMi | CLU_064864_1_0_1 |
InParanoidi | Q9H8W4 |
OMAi | GEHAAVW |
OrthoDBi | 1237900at2759 |
PhylomeDBi | Q9H8W4 |
TreeFami | TF315235 |
Family and domain databases
CDDi | cd01218, PH_Phafin2-like, 1 hit |
Gene3Di | 2.30.29.30, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR037871, PH_Phafin IPR000306, Znf_FYVE IPR017455, Znf_FYVE-rel IPR011011, Znf_FYVE_PHD IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF01363, FYVE, 1 hit PF00169, PH, 1 hit |
SMARTi | View protein in SMART SM00064, FYVE, 1 hit SM00233, PH, 1 hit |
SUPFAMi | SSF57903, SSF57903, 1 hit |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit PS50178, ZF_FYVE, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9H8W4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVDRLANSEA NTRRISIVEN CFGAAGQPLT IPGRVLIGEG VLTKLCRKKP
60 70 80 90 100
KARQFFLFND ILVYGNIVIQ KKKYNKQHII PLENVTIDSI KDEGDLRNGW
110 120 130 140 150
LIKTPTKSFA VYAATATEKS EWMNHINKCV TDLLSKSGKT PSNEHAAVWV
160 170 180 190 200
PDSEATVCMR CQKAKFTPVN RRHHCRKCGF VVCGPCSEKR FLLPSQSSKP
210 220 230 240
VRICDFCYDL LSAGDMATCQ PARSDSYSQS LKSPLNDMSD DDDDDDSSD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF434819 mRNA Translation: AAL30774.1 AK023249 mRNA Translation: BAB14486.1 AL834473 mRNA Translation: CAD39132.1 BC011806 mRNA Translation: AAH11806.1 |
CCDSi | CCDS6267.1 |
RefSeqi | NP_078889.1, NM_024613.3 |
Genome annotation databases
Ensembli | ENST00000315367; ENSP00000322373; ENSG00000175895 ENST00000519516; ENSP00000427792; ENSG00000175895 |
GeneIDi | 79666 |
KEGGi | hsa:79666 |
UCSCi | uc003yhn.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF434819 mRNA Translation: AAL30774.1 AK023249 mRNA Translation: BAB14486.1 AL834473 mRNA Translation: CAD39132.1 BC011806 mRNA Translation: AAH11806.1 |
CCDSi | CCDS6267.1 |
RefSeqi | NP_078889.1, NM_024613.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 122791, 174 interactors |
IntActi | Q9H8W4, 168 interactors |
MINTi | Q9H8W4 |
STRINGi | 9606.ENSP00000322373 |
PTM databases
iPTMneti | Q9H8W4 |
PhosphoSitePlusi | Q9H8W4 |
Genetic variation databases
BioMutai | PLEKHF2 |
DMDMi | 74762744 |
Proteomic databases
EPDi | Q9H8W4 |
jPOSTi | Q9H8W4 |
MassIVEi | Q9H8W4 |
MaxQBi | Q9H8W4 |
PaxDbi | Q9H8W4 |
PeptideAtlasi | Q9H8W4 |
PRIDEi | Q9H8W4 |
ProteomicsDBi | 81249 |
Protocols and materials databases
Antibodypediai | 25937, 120 antibodies |
DNASUi | 79666 |
Genome annotation databases
Ensembli | ENST00000315367; ENSP00000322373; ENSG00000175895 ENST00000519516; ENSP00000427792; ENSG00000175895 |
GeneIDi | 79666 |
KEGGi | hsa:79666 |
UCSCi | uc003yhn.3, human |
Organism-specific databases
CTDi | 79666 |
GeneCardsi | PLEKHF2 |
HGNCi | HGNC:20757, PLEKHF2 |
HPAi | ENSG00000175895, Low tissue specificity |
MIMi | 615208, gene |
neXtProti | NX_Q9H8W4 |
OpenTargetsi | ENSG00000175895 |
PharmGKBi | PA128394715 |
VEuPathDBi | HostDB:ENSG00000175895.3 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1729, Eukaryota |
GeneTreei | ENSGT00940000156408 |
HOGENOMi | CLU_064864_1_0_1 |
InParanoidi | Q9H8W4 |
OMAi | GEHAAVW |
OrthoDBi | 1237900at2759 |
PhylomeDBi | Q9H8W4 |
TreeFami | TF315235 |
Enzyme and pathway databases
PathwayCommonsi | Q9H8W4 |
SIGNORi | Q9H8W4 |
Miscellaneous databases
BioGRID-ORCSi | 79666, 14 hits in 997 CRISPR screens |
ChiTaRSi | PLEKHF2, human |
GeneWikii | PLEKHF2 |
GenomeRNAii | 79666 |
Pharosi | Q9H8W4, Tbio |
PROi | PR:Q9H8W4 |
RNActi | Q9H8W4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000175895, Expressed in secondary oocyte and 220 other tissues |
Genevisiblei | Q9H8W4, HS |
Family and domain databases
CDDi | cd01218, PH_Phafin2-like, 1 hit |
Gene3Di | 2.30.29.30, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR037871, PH_Phafin IPR000306, Znf_FYVE IPR017455, Znf_FYVE-rel IPR011011, Znf_FYVE_PHD IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF01363, FYVE, 1 hit PF00169, PH, 1 hit |
SMARTi | View protein in SMART SM00064, FYVE, 1 hit SM00233, PH, 1 hit |
SUPFAMi | SSF57903, SSF57903, 1 hit |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit PS50178, ZF_FYVE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PKHF2_HUMAN | |
Accessioni | Q9H8W4Primary (citable) accession number: Q9H8W4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2006 |
Last sequence update: | March 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot