UniProtKB - Q9H8V3 (ECT2_HUMAN)
Protein ECT2
ECT2
Functioni
Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
16 PublicationsActivity regulationi
GO - Molecular functioni
- GTPase activator activity Source: UniProtKB
- guanyl-nucleotide exchange factor activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- small GTPase binding Source: Ensembl
GO - Biological processi
- activation of GTPase activity Source: UniProtKB
- activation of protein kinase activity Source: UniProtKB
- bicellular tight junction assembly Source: UniProtKB
- cell differentiation Source: UniProtKB-KW
- cell morphogenesis Source: Ensembl
- cellular response to calcium ion Source: UniProtKB
- cellular response to hydrogen peroxide Source: UniProtKB
- cellular response to ionizing radiation Source: UniProtKB
- intracellular signal transduction Source: InterPro
- mitotic cytokinesis Source: UniProtKB
- nervous system development Source: GO_Central
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of cytokinesis Source: UniProtKB
- positive regulation of GTPase activity Source: UniProtKB
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- positive regulation of neuron differentiation Source: UniProtKB
- positive regulation of protein import into nucleus Source: UniProtKB
- protein homooligomerization Source: UniProtKB
- protein transport Source: UniProtKB-KW
- regulation of attachment of spindle microtubules to kinetochore Source: UniProtKB
- regulation of cytokinesis, actomyosin contractile ring assembly Source: InterPro
- regulation of protein kinase activity Source: UniProtKB
- regulation of small GTPase mediated signal transduction Source: Reactome
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Biological process | Cell cycle, Cell division, Differentiation, Neurogenesis, Protein transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q9H8V3 |
Reactomei | R-HSA-193648, NRAGE signals death through JNK R-HSA-416482, G alpha (12/13) signalling events R-HSA-8980692, RHOA GTPase cycle R-HSA-9013026, RHOB GTPase cycle R-HSA-9013148, CDC42 GTPase cycle R-HSA-9013149, RAC1 GTPase cycle |
SignaLinki | Q9H8V3 |
SIGNORi | Q9H8V3 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein ECT2Alternative name(s): Epithelial cell-transforming sequence 2 oncogene |
Gene namesi | Name:ECT2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:3155, ECT2 |
MIMi | 600586, gene |
neXtProti | NX_Q9H8V3 |
VEuPathDBi | HostDB:ENSG00000114346 |
Subcellular locationi
Cytoplasm and Cytosol
Cytoskeleton
Nucleus
Other locations
Note: Sequestered within the nucleus during interphase. Dispersed throughout the cytoplasm upon breakdown of the nuclear envelope during mitosis. Colocalizes with the centralspindlin complex to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalized with RhoA at the midbody. Its subcellular localization to tight junction is increased by calcium. Localized predominantly in the cytoplasm of numerous carcinoma cells.
Cytoskeleton
- centralspindlin complex Source: UniProtKB
- mitotic spindle Source: UniProtKB
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear body Source: HPA
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Plasma Membrane
- cleavage furrow Source: UniProtKB
Other locations
- bicellular tight junction Source: UniProtKB
- cell cortex Source: GO_Central
- cell-cell junction Source: UniProtKB
- cytoplasm Source: UniProtKB
- midbody Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Cytoplasm, Cytoskeleton, Nucleus, Tight junctionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 184 | T → A: Inhibits interaction with RACGAP1. Abolishes targeting to the central spindle. 2 Publications | 1 | |
Mutagenesisi | 226 | K → A: Inhibits interaction with RACGAP1. Abolishes targeting to the central spindle. 2 Publications | 1 | |
Mutagenesisi | 336 | W → R: Inhibits homodimerization. Increases binding with RhoA and GEF activity. 1 Publication | 1 | |
Mutagenesisi | 338 | W → A: 2-fold increase in GEF activity. 1 Publication | 1 | |
Mutagenesisi | 359 | T → A: Inhibits its phosphorylation and anchorage-independent growth and invasion in cancer cells. Does not inhibit its GEF activity. 1 Publication | 1 | |
Mutagenesisi | 373 | T → A: Does not inhibit its Rho exchange activity. Increases interaction with RACGAP1. Does not inhibit anchorage-independent growth and invasion in cancer cells. 2 Publications | 1 | |
Mutagenesisi | 373 | T → D: Does not inhibit subcellular localization or homodimerization. Enhances its Rho exchange activity. 2 Publications | 1 | |
Mutagenesisi | 379 – 381 | KRR → AAA: Shows both nuclear and cytoplasmic localization and activates its transforming activity. 1 Publication | 3 | |
Mutagenesisi | 402 – 404 | RKR → AKA: Shows both nuclear and cytoplasmic localization and activates its transforming activity. 1 Publication | 3 | |
Mutagenesisi | 444 | T → A: Diminishes its phosphorylation status. Reduces its interaction with PLK1 and Rho exchange activity. Does not change its subcellular localization. Does not inhibit anchorage-independent growth and invasion in cancer cells. 1 Publication | 1 | |
Mutagenesisi | 444 | T → D: Does not reduce its interaction with PLK1, change its subcellular localization and Rho exchange activity. 1 Publication | 1 | |
Mutagenesisi | 488 | R → C: Partially releases inhibition. 1 Publication | 1 | |
Mutagenesisi | 570 | R → D: 2-fold increase in GEF activity. 1 Publication | 1 | |
Mutagenesisi | 573 | A → D: 2-fold increase in GEF activity. 1 Publication | 1 | |
Mutagenesisi | 586 | R → Q: Complete loss of GEF activity and failure to support cytokinesis. 1 Publication | 1 | |
Mutagenesisi | 596 – 599 | PVQR → AAAA: Inhibits activation of the transforming activity. 1 Publication | 4 | |
Mutagenesisi | 601 | P → S: Complete loss of GEF activity and failure to support cytokinesis. 1 Publication | 1 | |
Mutagenesisi | 652 | F → A: No effect on GEF activity but severely decreases ECT2 activation by RHOA. Localizes correctly but fails to rescue the cytokinesis defect caused by knockdown of endogenous ECT2 in contrast to the wild-type protein which rescues this defect. 1 Publication | 1 | |
Mutagenesisi | 656 | Y → A: No effect on GEF activity but severely decreases ECT2 activation by RHOA. Fails to rescue the cytokinesis defect caused by knockdown of endogenous ECT2 in contrast to the wild-type protein which rescues this defect. 1 Publication | 1 | |
Mutagenesisi | 670 | R → Q: Marked increase in GEF activity and rescues the cytokinesis defect caused by depletion of endogenous ECT2. When expressed in a cancer cell line, causes faster cell proliferation than the wild-type protein. 1 Publication | 1 | |
Mutagenesisi | 734 | P → D: More than 10-fold increase in GEF activity. Overexpression causes noticeable changes in interphase cell morphology such as cell rounding and formation of stress fibers, suggesting ectopic RhoA activation. 1 Publication | 1 | |
Mutagenesisi | 796 | C → K: More than 10-fold increase in GEF activity. Overexpression causes noticeable changes in interphase cell morphology such as cell rounding and formation of stress fibers, suggesting ectopic RHOA activation. 1 Publication | 1 | |
Mutagenesisi | 846 | T → A: Diminishes its phosphorylation status. 1 Publication | 1 |
Keywords - Diseasei
OncogeneOrganism-specific databases
DisGeNETi | 1894 |
OpenTargetsi | ENSG00000114346 |
PharmGKBi | PA27600 |
Miscellaneous databases
Pharosi | Q9H8V3, Tbio |
Genetic variation databases
BioMutai | ECT2 |
DMDMi | 357529579 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000080938 | 2 – 914 | Protein ECT2Add BLAST | 913 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 359 | Phosphothreonine; by PKC/PRKCICombined sources1 Publication | 1 | |
Modified residuei | 367 | PhosphoserineCombined sources | 1 | |
Modified residuei | 370 | PhosphoserineCombined sources | 1 | |
Modified residuei | 373 | Phosphothreonine; by CDK1Combined sources1 Publication | 1 | |
Modified residuei | 376 | PhosphoserineCombined sources | 1 | |
Modified residuei | 444 | Phosphothreonine; by CDK11 Publication | 1 | |
Cross-linki | 611 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 716 | PhosphoserineCombined sources | 1 | |
Modified residuei | 842 | PhosphoserineCombined sources | 1 | |
Modified residuei | 846 | Phosphothreonine; by CDK1Combined sources | 1 | |
Modified residuei | 861 | PhosphoserineCombined sources | 1 | |
Modified residuei | 866 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9H8V3 |
jPOSTi | Q9H8V3 |
MassIVEi | Q9H8V3 |
MaxQBi | Q9H8V3 |
PaxDbi | Q9H8V3 |
PeptideAtlasi | Q9H8V3 |
PRIDEi | Q9H8V3 |
ProteomicsDBi | 81243 [Q9H8V3-1] 81244 [Q9H8V3-2] 81245 [Q9H8V3-3] |
PTM databases
iPTMneti | Q9H8V3 |
MetOSitei | Q9H8V3 |
PhosphoSitePlusi | Q9H8V3 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000114346, Expressed in secondary oocyte and 206 other tissues |
ExpressionAtlasi | Q9H8V3, baseline and differential |
Genevisiblei | Q9H8V3, HS |
Organism-specific databases
HPAi | ENSG00000114346, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer (PubMed:15545273, PubMed:14645260, PubMed:16170345). Homooligomer (PubMed:15545273).
Found in the centralspindlin complex (PubMed:16236794).
Interacts with NR1I3 (By similarity).
Interacts (Thr-359 phosphorylated form) with PARD6A; the interaction is observed in cancer cells (PubMed:15254234, PubMed:19617897).
Interacts (Thr-359 phosphorylated form) with PRKCI; the interaction is observed in cancer cells (PubMed:19617897).
Interacts with PKP4; the interaction is observed at the midbody (PubMed:22814378).
Interacts with RACGAP1/CYK4; the interaction is direct, occurs in a microtubule-dependent manner, occurs at anaphase and during cytokinesis, is inhibited in metaphase by phosphorylation of ECT2 on Thr-373 and is stimulated in early anaphase by dephosphorylation of ECT2 probably on Thr-373 through CDK1 activity (PubMed:16103226, PubMed:16129829, PubMed:16236794, PubMed:19468300, PubMed:19468302, PubMed:25068414).
Interacts with PLK1; the interaction is stimulated upon its phosphorylation on Thr-444 (PubMed:16247472).
Interacts with RHOA; the interaction results in allosteric activation of ECT2 (PubMed:31888991).
Interacts with KIF23, PARD3, PARD6B and PRKCQ (PubMed:15254234, PubMed:16236794).
By similarity14 PublicationsBinary interactionsi
Q9H8V3
GO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
- small GTPase binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 108223, 870 interactors |
CORUMi | Q9H8V3 |
DIPi | DIP-47496N |
IntActi | Q9H8V3, 23 interactors |
MINTi | Q9H8V3 |
STRINGi | 9606.ENSP00000376457 |
Miscellaneous databases
RNActi | Q9H8V3, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9H8V3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9H8V3 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 171 – 260 | BRCT 1PROSITE-ProRule annotationAdd BLAST | 90 | |
Domaini | 266 – 354 | BRCT 2PROSITE-ProRule annotationAdd BLAST | 89 | |
Domaini | 452 – 641 | DHPROSITE-ProRule annotationAdd BLAST | 190 | |
Domaini | 675 – 794 | PHAdd BLAST | 120 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 388 – 449 | DisorderedSequence analysisAdd BLAST | 62 | |
Regioni | 857 – 884 | DisorderedSequence analysisAdd BLAST | 28 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 378 – 382 | Nuclear localization signal | 5 | |
Motifi | 401 – 405 | Nuclear localization signal | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 413 – 449 | Polar residuesSequence analysisAdd BLAST | 37 | |
Compositional biasi | 861 – 884 | Polar residuesSequence analysisAdd BLAST | 24 |
Domaini
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3524, Eukaryota |
GeneTreei | ENSGT00940000156299 |
HOGENOMi | CLU_008187_0_0_1 |
InParanoidi | Q9H8V3 |
OMAi | CKAKESN |
OrthoDBi | 557169at2759 |
TreeFami | TF101161 |
Family and domain databases
CDDi | cd00160, RhoGEF, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 1 hit 3.40.50.10190, 3 hits |
InterProi | View protein in InterPro IPR001357, BRCT_dom IPR036420, BRCT_dom_sf IPR035899, DBL_dom_sf IPR000219, DH-domain IPR026817, Ect2 IPR001331, GDS_CDC24_CS IPR011993, PH-like_dom_sf |
PANTHERi | PTHR16777, PTHR16777, 1 hit |
Pfami | View protein in Pfam PF00533, BRCT, 1 hit PF12738, PTCB-BRCT, 1 hit PF00621, RhoGEF, 1 hit |
SMARTi | View protein in SMART SM00292, BRCT, 2 hits SM00325, RhoGEF, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit SSF52113, SSF52113, 2 hits |
PROSITEi | View protein in PROSITE PS50172, BRCT, 2 hits PS00741, DH_1, 1 hit PS50010, DH_2, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAENSVLTST TGRTSLADSS IFDSKVTEIS KENLLIGSTS YVEEEMPQIE
60 70 80 90 100
TRVILVQEAG KQEELIKALK TIKIMEVPVI KIKESCPGKS DEKLIKSVIN
110 120 130 140 150
MDIKVGFVKM ESVEEFEGLD SPEFENVFVV TDFQDSVFND LYKADCRVIG
160 170 180 190 200
PPVVLNCSQK GEPLPFSCRP LYCTSMMNLV LCFTGFRKKE ELVRLVTLVH
210 220 230 240 250
HMGGVIRKDF NSKVTHLVAN CTQGEKFRVA VSLGTPIMKP EWIYKAWERR
260 270 280 290 300
NEQDFYAAVD DFRNEFKVPP FQDCILSFLG FSDEEKTNME EMTEMQGGKY
310 320 330 340 350
LPLGDERCTH LVVEENIVKD LPFEPSKKLY VVKQEWFWGS IQMDARAGET
360 370 380 390 400
MYLYEKANTP ELKKSVSMLS LNTPNSNRKR RRLKETLAQL SRETDVSPFP
410 420 430 440 450
PRKRPSAEHS LSIGSLLDIS NTPESSINYG DTPKSCTKSS KSSTPVPSKQ
460 470 480 490 500
SARWQVAKEL YQTESNYVNI LATIIQLFQV PLEEEGQRGG PILAPEEIKT
510 520 530 540 550
IFGSIPDIFD VHTKIKDDLE DLIVNWDESK SIGDIFLKYS KDLVKTYPPF
560 570 580 590 600
VNFFEMSKET IIKCEKQKPR FHAFLKINQA KPECGRQSLV ELLIRPVQRL
610 620 630 640 650
PSVALLLNDL KKHTADENPD KSTLEKAIGS LKEVMTHINE DKRKTEAQKQ
660 670 680 690 700
IFDVVYEVDG CPANLLSSHR SLVQRVETIS LGEHPCDRGE QVTLFLFNDC
710 720 730 740 750
LEIARKRHKV IGTFRSPHGQ TRPPASLKHI HLMPLSQIKK VLDIRETEDC
760 770 780 790 800
HNAFALLVRP PTEQANVLLS FQMTSDELPK ENWLKMLCRH VANTICKADA
810 820 830 840 850
ENLIYTADPE SFEVNTKDMD STLSRASRAI KKTSKKVTRA FSFSKTPKRA
860 870 880 890 900
LRRALMTSHG SVEGRSPSSN DKHVMSRLSS TSSLAGIPSP SLVSLPSFFE
910
RRSHTLSRST THLI
The sequence of this isoform differs from the canonical sequence as follows:
886-914: GIPSPSLVSLPSFFERRSHTLSRSTTHLI → ITHSVSTSNV...SKSSLTFVKN
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH7C3G1 | H7C3G1_HUMAN | Protein ECT2 | ECT2 | 209 | Annotation score: | ||
C9JDB4 | C9JDB4_HUMAN | Protein ECT2 | ECT2 | 204 | Annotation score: | ||
C9JTI2 | C9JTI2_HUMAN | Protein ECT2 | ECT2 | 150 | Annotation score: | ||
C9J0L6 | C9J0L6_HUMAN | Protein ECT2 | ECT2 | 106 | Annotation score: | ||
H7C103 | H7C103_HUMAN | Protein ECT2 | ECT2 | 298 | Annotation score: | ||
C9J1C4 | C9J1C4_HUMAN | Protein ECT2 | ECT2 | 159 | Annotation score: | ||
C9JDV9 | C9JDV9_HUMAN | Protein ECT2 | ECT2 | 110 | Annotation score: | ||
C9JB41 | C9JB41_HUMAN | Protein ECT2 | ECT2 | 18 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 66 | I → T in BAB14498 (PubMed:16641997).Curated | 1 | |
Sequence conflicti | 276 | L → F in BAB14498 (PubMed:16641997).Curated | 1 | |
Sequence conflicti | 393 | E → D in BAB14498 (PubMed:16641997).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_047064 | 15 | S → T. Corresponds to variant dbSNP:rs34703432Ensembl. | 1 | |
Natural variantiVAR_035975 | 833 | T → P in a breast cancer sample; somatic mutation. 1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041976 | 44 | Missing in isoform 2. 4 Publications | 1 | |
Alternative sequenceiVSP_041977 | 71 – 101 | Missing in isoform 2 and isoform 4. 4 PublicationsAdd BLAST | 31 | |
Alternative sequenceiVSP_041978 | 886 – 914 | GIPSP…TTHLI → ITHSVSTSNVIGFTKHVYVQ RLNSTGGRSQYSWFQSVRHS AFRASFSEILEGNTDFSNFK KVLSKSSLTFVKN in isoform 3. 1 PublicationAdd BLAST | 29 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY376439 mRNA Translation: AAQ83675.1 DQ847274 mRNA Translation: ABH10140.1 AK001323 mRNA Translation: BAA91624.1 Different initiation. AK023267 mRNA Translation: BAB14498.1 AK314581 mRNA Translation: BAG37157.1 AC108667 Genomic DNA No translation available. BC112086 mRNA Translation: AAI12087.1 AL137710 mRNA Translation: CAB70886.1 |
CCDSi | CCDS3220.1 [Q9H8V3-4] CCDS58860.1 [Q9H8V3-1] CCDS87168.1 [Q9H8V3-2] |
RefSeqi | NP_001245244.1, NM_001258315.1 [Q9H8V3-1] NP_001245245.1, NM_001258316.1 [Q9H8V3-4] NP_060568.3, NM_018098.5 [Q9H8V3-4] XP_011510816.1, XM_011512514.2 [Q9H8V3-3] |
Genome annotation databases
Ensembli | ENST00000232458; ENSP00000232458; ENSG00000114346 [Q9H8V3-4] ENST00000392692; ENSP00000376457; ENSG00000114346 ENST00000417960; ENSP00000415876; ENSG00000114346 [Q9H8V3-2] ENST00000441497; ENSP00000412259; ENSG00000114346 [Q9H8V3-4] ENST00000540509; ENSP00000443160; ENSG00000114346 [Q9H8V3-4] |
GeneIDi | 1894 |
KEGGi | hsa:1894 |
MANE-Selecti | ENST00000392692.8; ENSP00000376457.3; NM_001258315.2; NP_001245244.1 |
UCSCi | uc003fih.4, human [Q9H8V3-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY376439 mRNA Translation: AAQ83675.1 DQ847274 mRNA Translation: ABH10140.1 AK001323 mRNA Translation: BAA91624.1 Different initiation. AK023267 mRNA Translation: BAB14498.1 AK314581 mRNA Translation: BAG37157.1 AC108667 Genomic DNA No translation available. BC112086 mRNA Translation: AAI12087.1 AL137710 mRNA Translation: CAB70886.1 |
CCDSi | CCDS3220.1 [Q9H8V3-4] CCDS58860.1 [Q9H8V3-1] CCDS87168.1 [Q9H8V3-2] |
RefSeqi | NP_001245244.1, NM_001258315.1 [Q9H8V3-1] NP_001245245.1, NM_001258316.1 [Q9H8V3-4] NP_060568.3, NM_018098.5 [Q9H8V3-4] XP_011510816.1, XM_011512514.2 [Q9H8V3-3] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3L46 | X-ray | 1.48 | A/B | 268-361 | [»] | |
4N40 | X-ray | 3.11 | A | 45-356 | [»] | |
6L30 | X-ray | 2.80 | A | 173-865 | [»] | |
SMRi | Q9H8V3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 108223, 870 interactors |
CORUMi | Q9H8V3 |
DIPi | DIP-47496N |
IntActi | Q9H8V3, 23 interactors |
MINTi | Q9H8V3 |
STRINGi | 9606.ENSP00000376457 |
PTM databases
iPTMneti | Q9H8V3 |
MetOSitei | Q9H8V3 |
PhosphoSitePlusi | Q9H8V3 |
Genetic variation databases
BioMutai | ECT2 |
DMDMi | 357529579 |
Proteomic databases
EPDi | Q9H8V3 |
jPOSTi | Q9H8V3 |
MassIVEi | Q9H8V3 |
MaxQBi | Q9H8V3 |
PaxDbi | Q9H8V3 |
PeptideAtlasi | Q9H8V3 |
PRIDEi | Q9H8V3 |
ProteomicsDBi | 81243 [Q9H8V3-1] 81244 [Q9H8V3-2] 81245 [Q9H8V3-3] |
Protocols and materials databases
Antibodypediai | 33733, 378 antibodies from 32 providers |
DNASUi | 1894 |
Genome annotation databases
Ensembli | ENST00000232458; ENSP00000232458; ENSG00000114346 [Q9H8V3-4] ENST00000392692; ENSP00000376457; ENSG00000114346 ENST00000417960; ENSP00000415876; ENSG00000114346 [Q9H8V3-2] ENST00000441497; ENSP00000412259; ENSG00000114346 [Q9H8V3-4] ENST00000540509; ENSP00000443160; ENSG00000114346 [Q9H8V3-4] |
GeneIDi | 1894 |
KEGGi | hsa:1894 |
MANE-Selecti | ENST00000392692.8; ENSP00000376457.3; NM_001258315.2; NP_001245244.1 |
UCSCi | uc003fih.4, human [Q9H8V3-1] |
Organism-specific databases
CTDi | 1894 |
DisGeNETi | 1894 |
GeneCardsi | ECT2 |
HGNCi | HGNC:3155, ECT2 |
HPAi | ENSG00000114346, Low tissue specificity |
MIMi | 600586, gene |
neXtProti | NX_Q9H8V3 |
OpenTargetsi | ENSG00000114346 |
PharmGKBi | PA27600 |
VEuPathDBi | HostDB:ENSG00000114346 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3524, Eukaryota |
GeneTreei | ENSGT00940000156299 |
HOGENOMi | CLU_008187_0_0_1 |
InParanoidi | Q9H8V3 |
OMAi | CKAKESN |
OrthoDBi | 557169at2759 |
TreeFami | TF101161 |
Enzyme and pathway databases
PathwayCommonsi | Q9H8V3 |
Reactomei | R-HSA-193648, NRAGE signals death through JNK R-HSA-416482, G alpha (12/13) signalling events R-HSA-8980692, RHOA GTPase cycle R-HSA-9013026, RHOB GTPase cycle R-HSA-9013148, CDC42 GTPase cycle R-HSA-9013149, RAC1 GTPase cycle |
SignaLinki | Q9H8V3 |
SIGNORi | Q9H8V3 |
Miscellaneous databases
BioGRID-ORCSi | 1894, 627 hits in 1057 CRISPR screens |
ChiTaRSi | ECT2, human |
EvolutionaryTracei | Q9H8V3 |
GeneWikii | ECT2 |
GenomeRNAii | 1894 |
Pharosi | Q9H8V3, Tbio |
PROi | PR:Q9H8V3 |
RNActi | Q9H8V3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000114346, Expressed in secondary oocyte and 206 other tissues |
ExpressionAtlasi | Q9H8V3, baseline and differential |
Genevisiblei | Q9H8V3, HS |
Family and domain databases
CDDi | cd00160, RhoGEF, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 1 hit 3.40.50.10190, 3 hits |
InterProi | View protein in InterPro IPR001357, BRCT_dom IPR036420, BRCT_dom_sf IPR035899, DBL_dom_sf IPR000219, DH-domain IPR026817, Ect2 IPR001331, GDS_CDC24_CS IPR011993, PH-like_dom_sf |
PANTHERi | PTHR16777, PTHR16777, 1 hit |
Pfami | View protein in Pfam PF00533, BRCT, 1 hit PF12738, PTCB-BRCT, 1 hit PF00621, RhoGEF, 1 hit |
SMARTi | View protein in SMART SM00292, BRCT, 2 hits SM00325, RhoGEF, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit SSF52113, SSF52113, 2 hits |
PROSITEi | View protein in PROSITE PS50172, BRCT, 2 hits PS00741, DH_1, 1 hit PS50010, DH_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ECT2_HUMAN | |
Accessioni | Q9H8V3Primary (citable) accession number: Q9H8V3 Secondary accession number(s): Q0MT80 Q9NVW9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2001 |
Last sequence update: | November 16, 2011 | |
Last modified: | February 23, 2022 | |
This is version 177 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references