UniProtKB - Q9H8S9 (MOB1A_HUMAN)
Protein
MOB kinase activator 1A
Gene
MOB1A
Organism
Homo sapiens (Human)
Status
Functioni
Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L. Acts cooperatively with STK3/MST2 to activate STK38.3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 79 | Zinc1 Publication | 1 | |
Metal bindingi | 84 | Zinc1 Publication | 1 | |
Metal bindingi | 161 | Zinc1 Publication | 1 | |
Metal bindingi | 166 | Zinc1 Publication | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- hippo signaling Source: UniProtKB
Keywordsi
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9H8S9 |
Reactomei | R-HSA-2028269, Signaling by Hippo |
SIGNORi | Q9H8S9 |
Names & Taxonomyi
Protein namesi | Recommended name: MOB kinase activator 1AAlternative name(s): Mob1 alpha Short name: Mob1A Mob1 homolog 1B Mps one binder kinase activator-like 1B |
Gene namesi | Name:MOB1A Synonyms:C2orf6, MOB4B, MOBK1B, MOBKL1B |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000114978.17 |
HGNCi | HGNC:16015, MOB1A |
MIMi | 609281, gene |
neXtProti | NX_Q9H8S9 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- nucleolus Source: HPA
- nucleoplasm Source: HPA
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 55233 |
OpenTargetsi | ENSG00000114978 |
PharmGKBi | PA25894 |
Miscellaneous databases
Pharosi | Q9H8S9, Tbio |
Polymorphism and mutation databases
BioMutai | MOB1A |
DMDMi | 56749356 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000193566 | 2 – 216 | MOB kinase activator 1AAdd BLAST | 215 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources1 Publication | 1 | |
Modified residuei | 12 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 35 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 74 | Phosphothreonine; by STK3/MST21 Publication | 1 | |
Modified residuei | 181 | Phosphothreonine1 Publication | 1 |
Post-translational modificationi
Phosphorylated by STK3/MST2 and STK4/MST1 and this phosphorylation enhances its binding to LATS1.3 Publications
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9H8S9 |
jPOSTi | Q9H8S9 |
MassIVEi | Q9H8S9 |
MaxQBi | Q9H8S9 |
PaxDbi | Q9H8S9 |
PeptideAtlasi | Q9H8S9 |
PRIDEi | Q9H8S9 |
ProteomicsDBi | 81237 [Q9H8S9-1] 81238 [Q9H8S9-2] |
2D gel databases
OGPi | Q9H8S9 |
PTM databases
iPTMneti | Q9H8S9 |
PhosphoSitePlusi | Q9H8S9 |
Expressioni
Tissue specificityi
Adrenal gland, bone marrow, brain, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, heart, spinal cord, fetal brain and fetal liver.1 Publication
Gene expression databases
Bgeei | ENSG00000114978, Expressed in leukocyte and 257 other tissues |
Genevisiblei | Q9H8S9, HS |
Organism-specific databases
HPAi | ENSG00000114978, Low tissue specificity |
Interactioni
Subunit structurei
Binds STK38 and STK38L.
Interacts with LATS1 and LATS2.
Forms a tripartite complex with STK38 and STK3/MST2.
3 PublicationsBinary interactionsi
Hide detailsQ9H8S9
Protein-protein interaction databases
BioGRIDi | 120527, 63 interactors |
DIPi | DIP-36594N |
IntActi | Q9H8S9, 54 interactors |
MINTi | Q9H8S9 |
STRINGi | 9606.ENSP00000379364 |
Miscellaneous databases
RNActi | Q9H8S9, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9H8S9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9H8S9 |
Family & Domainsi
Sequence similaritiesi
Belongs to the MOB1/phocein family.Curated
Phylogenomic databases
eggNOGi | KOG0440, Eukaryota |
GeneTreei | ENSGT01000000214382 |
HOGENOMi | CLU_038321_3_1_1 |
InParanoidi | Q9H8S9 |
OMAi | TCEVMSA |
OrthoDBi | 1127941at2759 |
PhylomeDBi | Q9H8S9 |
TreeFami | TF300789 |
Family and domain databases
Gene3Di | 1.20.140.30, 1 hit |
InterProi | View protein in InterPro IPR005301, MOB_kinase_act_fam IPR036703, MOB_kinase_act_sf |
PANTHERi | PTHR22599, PTHR22599, 1 hit |
Pfami | View protein in Pfam PF03637, Mob1_phocein, 1 hit |
SMARTi | View protein in SMART SM01388, Mob1_phocein, 1 hit |
SUPFAMi | SSF101152, SSF101152, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q9H8S9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSFLFSSRSS KTFKPKKNIP EGSHQYELLK HAEATLGSGN LRQAVMLPEG
60 70 80 90 100
EDLNEWIAVN TVDFFNQINM LYGTITEFCT EASCPVMSAG PRYEYHWADG
110 120 130 140 150
TNIKKPIKCS APKYIDYLMT WVQDQLDDET LFPSKIGVPF PKNFMSVAKT
160 170 180 190 200
ILKRLFRVYA HIYHQHFDSV MQLQEEAHLN TSFKHFIFFV QEFNLIDRRE
210
LAPLQELIEK LGSKDR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 5 | F → L in BAB14525 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 176 | E → G in BAA91810 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_012295 | 138 – 148 | VPFPKNFMSVA → ELTLSKYSFFF in isoform 2. 1 PublicationAdd BLAST | 11 | |
Alternative sequenceiVSP_012296 | 149 – 216 | Missing in isoform 2. 1 PublicationAdd BLAST | 68 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB016839 mRNA Translation: BAB19058.1 AJ577474 mRNA Translation: CAE12093.1 AK001650 mRNA Translation: BAA91810.1 AK021657 mRNA Translation: BAB13868.1 AK023321 mRNA Translation: BAB14525.1 AC073263 Genomic DNA Translation: AAX93060.1 BC003398 mRNA Translation: AAH03398.1 |
CCDSi | CCDS46340.1 [Q9H8S9-1] |
RefSeqi | NP_001304039.1, NM_001317110.1 NP_001304040.1, NM_001317111.1 NP_001304041.1, NM_001317112.1 NP_060691.2, NM_018221.4 [Q9H8S9-1] |
Genome annotation databases
Ensembli | ENST00000396049; ENSP00000379364; ENSG00000114978 [Q9H8S9-1] |
GeneIDi | 55233 |
KEGGi | hsa:55233 |
UCSCi | uc002skh.5, human [Q9H8S9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB016839 mRNA Translation: BAB19058.1 AJ577474 mRNA Translation: CAE12093.1 AK001650 mRNA Translation: BAA91810.1 AK021657 mRNA Translation: BAB13868.1 AK023321 mRNA Translation: BAB14525.1 AC073263 Genomic DNA Translation: AAX93060.1 BC003398 mRNA Translation: AAH03398.1 |
CCDSi | CCDS46340.1 [Q9H8S9-1] |
RefSeqi | NP_001304039.1, NM_001317110.1 NP_001304040.1, NM_001317111.1 NP_001304041.1, NM_001317112.1 NP_060691.2, NM_018221.4 [Q9H8S9-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1PI1 | X-ray | 2.00 | A | 33-216 | [»] | |
4J1V | X-ray | 1.95 | A/C | 33-216 | [»] | |
4JIZ | X-ray | 2.10 | A | 40-211 | [»] | |
5BRK | X-ray | 2.30 | A | 1-216 | [»] | |
5BRM | X-ray | 2.65 | A/B/C/D/E/F | 41-216 | [»] | |
5TWF | X-ray | 3.14 | A/B | 1-216 | [»] | |
5TWG | X-ray | 2.30 | A | 1-216 | [»] | |
5TWH | X-ray | 2.50 | A | 1-216 | [»] | |
5XQZ | X-ray | 2.10 | A/B | 33-216 | [»] | |
6MCP | X-ray | 2.50 | B/D | 33-216 | [»] | |
6MCQ | X-ray | 2.57 | B/D | 33-216 | [»] | |
SMRi | Q9H8S9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 120527, 63 interactors |
DIPi | DIP-36594N |
IntActi | Q9H8S9, 54 interactors |
MINTi | Q9H8S9 |
STRINGi | 9606.ENSP00000379364 |
PTM databases
iPTMneti | Q9H8S9 |
PhosphoSitePlusi | Q9H8S9 |
Polymorphism and mutation databases
BioMutai | MOB1A |
DMDMi | 56749356 |
2D gel databases
OGPi | Q9H8S9 |
Proteomic databases
EPDi | Q9H8S9 |
jPOSTi | Q9H8S9 |
MassIVEi | Q9H8S9 |
MaxQBi | Q9H8S9 |
PaxDbi | Q9H8S9 |
PeptideAtlasi | Q9H8S9 |
PRIDEi | Q9H8S9 |
ProteomicsDBi | 81237 [Q9H8S9-1] 81238 [Q9H8S9-2] |
Protocols and materials databases
Antibodypediai | 47474, 168 antibodies |
DNASUi | 55233 |
Genome annotation databases
Ensembli | ENST00000396049; ENSP00000379364; ENSG00000114978 [Q9H8S9-1] |
GeneIDi | 55233 |
KEGGi | hsa:55233 |
UCSCi | uc002skh.5, human [Q9H8S9-1] |
Organism-specific databases
CTDi | 55233 |
DisGeNETi | 55233 |
EuPathDBi | HostDB:ENSG00000114978.17 |
GeneCardsi | MOB1A |
HGNCi | HGNC:16015, MOB1A |
HPAi | ENSG00000114978, Low tissue specificity |
MIMi | 609281, gene |
neXtProti | NX_Q9H8S9 |
OpenTargetsi | ENSG00000114978 |
PharmGKBi | PA25894 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0440, Eukaryota |
GeneTreei | ENSGT01000000214382 |
HOGENOMi | CLU_038321_3_1_1 |
InParanoidi | Q9H8S9 |
OMAi | TCEVMSA |
OrthoDBi | 1127941at2759 |
PhylomeDBi | Q9H8S9 |
TreeFami | TF300789 |
Enzyme and pathway databases
PathwayCommonsi | Q9H8S9 |
Reactomei | R-HSA-2028269, Signaling by Hippo |
SIGNORi | Q9H8S9 |
Miscellaneous databases
BioGRID-ORCSi | 55233, 3 hits in 841 CRISPR screens |
ChiTaRSi | MOB1A, human |
EvolutionaryTracei | Q9H8S9 |
GeneWikii | MOBKL1B |
GenomeRNAii | 55233 |
Pharosi | Q9H8S9, Tbio |
PROi | PR:Q9H8S9 |
RNActi | Q9H8S9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000114978, Expressed in leukocyte and 257 other tissues |
Genevisiblei | Q9H8S9, HS |
Family and domain databases
Gene3Di | 1.20.140.30, 1 hit |
InterProi | View protein in InterPro IPR005301, MOB_kinase_act_fam IPR036703, MOB_kinase_act_sf |
PANTHERi | PTHR22599, PTHR22599, 1 hit |
Pfami | View protein in Pfam PF03637, Mob1_phocein, 1 hit |
SMARTi | View protein in SMART SM01388, Mob1_phocein, 1 hit |
SUPFAMi | SSF101152, SSF101152, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MOB1A_HUMAN | |
Accessioni | Q9H8S9Primary (citable) accession number: Q9H8S9 Secondary accession number(s): Q53S34 Q9NVE2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 21, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 171 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM