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Protein

Bromodomain-containing protein 9

Gene

BRD9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in chromatin remodeling and regulation of transcription (PubMed:22464331, PubMed:26365797). Acts as a chromatin reader that recognizes and binds acylated histones: binds histones that are acetylated and/or butyrylated (PubMed:26365797).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei169Histone H4K5ac H4K8ac and histone H4K5bu H4K8bu1 Publication1
Binding sitei222Histone H4K5ac H4K8ac and histone H4K5bu H4K8bu1 Publication1

GO - Molecular functioni

  • lysine-acetylated histone binding Source: UniProtKB
  • nucleic acid binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Bromodomain-containing protein 9
Alternative name(s):
Rhabdomyosarcoma antigen MU-RMS-40.8
Gene namesi
Name:BRD9
ORF Names:UNQ3040/PRO9856
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000028310.17
HGNCiHGNC:25818 BRD9
neXtProtiNX_Q9H8M2

Pathology & Biotechi

Organism-specific databases

DisGeNETi65980
OpenTargetsiENSG00000028310
PharmGKBiPA134866578

Chemistry databases

ChEMBLiCHEMBL3108640
GuidetoPHARMACOLOGYi2728

Polymorphism and mutation databases

BioMutaiBRD9
DMDMi239938605

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002392191 – 597Bromodomain-containing protein 9Add BLAST597

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei56PhosphoserineBy similarity1
Modified residuei373N6-acetyllysine; alternateCombined sources1
Cross-linki373Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei566PhosphoserineCombined sources1
Modified residuei588PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9H8M2
PaxDbiQ9H8M2
PeptideAtlasiQ9H8M2
PRIDEiQ9H8M2
ProteomicsDBi81218
81219 [Q9H8M2-1]
81220 [Q9H8M2-2]
81221 [Q9H8M2-3]
81222 [Q9H8M2-4]
81223 [Q9H8M2-6]
TopDownProteomicsiQ9H8M2-1 [Q9H8M2-1]
Q9H8M2-4 [Q9H8M2-4]

PTM databases

iPTMnetiQ9H8M2
PhosphoSitePlusiQ9H8M2

Expressioni

Gene expression databases

BgeeiENSG00000028310
CleanExiHS_BRD9
ExpressionAtlasiQ9H8M2 baseline and differential
GenevisibleiQ9H8M2 HS

Organism-specific databases

HPAiHPA021465
HPA023197

Interactioni

Subunit structurei

Binds acetylated histones H3 and H4 (PubMed:22464331, PubMed:26365797). Binds butyrylated histone H4 (PubMed:26365797).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CATIPQ7Z7H33EBI-10258305,EBI-10258233

GO - Molecular functioni

  • lysine-acetylated histone binding Source: UniProtKB

Protein-protein interaction databases

BioGridi122430, 13 interactors
IntActiQ9H8M2, 3 interactors
STRINGi9606.ENSP00000419765

Chemistry databases

BindingDBiQ9H8M2

Structurei

Secondary structure

1597
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi140 – 154Combined sources15
Turni160 – 162Combined sources3
Turni167 – 169Combined sources3
Helixi173 – 176Combined sources4
Helixi183 – 191Combined sources9
Helixi198 – 215Combined sources18
Beta strandi218 – 220Combined sources3
Helixi221 – 238Combined sources18
Helixi239 – 243Combined sources5
Turni247 – 249Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3HMEX-ray2.23A/B134-239[»]
4NQNX-ray1.73A134-239[»]
4UITX-ray1.30A134-238[»]
4UIUX-ray1.64A134-238[»]
4UIVX-ray1.72A134-238[»]
4UIWX-ray1.73A134-238[»]
4XY8X-ray1.70A134-239[»]
4YY4X-ray1.47A134-239[»]
4YY6X-ray1.45A134-239[»]
4YYDX-ray1.52A134-239[»]
4YYGX-ray2.10A134-239[»]
4YYHX-ray1.74A/B134-239[»]
4YYIX-ray1.50A/B/D/E134-239[»]
4YYJX-ray1.85A/B/D/E134-239[»]
4YYKX-ray1.79A/B/D/E134-239[»]
4Z6HX-ray1.80A/B134-239[»]
4Z6IX-ray1.95A/B134-239[»]
5E9VX-ray1.80A/B134-239[»]
5EU1X-ray1.60A/B134-239[»]
5F1HX-ray1.82A/B134-239[»]
5F1LX-ray2.30A/B134-239[»]
5F25X-ray1.68A/B134-239[»]
5F2PX-ray1.80A/B134-239[»]
5I40X-ray1.04A138-239[»]
5I7XX-ray1.18A138-238[»]
5I7YX-ray1.45A138-238[»]
5IGMX-ray1.60A/B134-239[»]
5IGNX-ray1.70A/B134-239[»]
5JI8X-ray1.42A137-239[»]
5MKYX-ray1.67A134-238[»]
5TWXX-ray2.55A/B/C/D134-250[»]
ProteinModelPortaliQ9H8M2
SMRiQ9H8M2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9H8M2

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini153 – 223BromoPROSITE-ProRule annotationAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni214 – 216Histone H4K5ac H4K8ac and histone H4K5bu H4K8bu1 Publication3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi64 – 95Lys-richAdd BLAST32

Domaini

The Bromo domain mediates interaction with histones that have acetylated lysine residues at specific positions (PubMed:22464331). Also recognizes and binds histones that are butyrylated (PubMed:26365797).2 Publications

Keywords - Domaini

Bromodomain

Phylogenomic databases

eggNOGiKOG1828 Eukaryota
ENOG410XRRI LUCA
GeneTreeiENSGT00530000063939
HOGENOMiHOG000070022
HOVERGENiHBG107536
InParanoidiQ9H8M2
KOiK22184
OMAiYSKKMVD
OrthoDBiEOG091G06S4
PhylomeDBiQ9H8M2
TreeFamiTF106439

Family and domain databases

Gene3Di1.20.920.10, 1 hit
InterProiView protein in InterPro
IPR001487 Bromodomain
IPR036427 Bromodomain-like_sf
IPR021900 DUF3512
PfamiView protein in Pfam
PF00439 Bromodomain, 1 hit
PF12024 DUF3512, 1 hit
PRINTSiPR00503 BROMODOMAIN
SMARTiView protein in SMART
SM00297 BROMO, 1 hit
SUPFAMiSSF47370 SSF47370, 1 hit
PROSITEiView protein in PROSITE
PS50014 BROMODOMAIN_2, 1 hit

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H8M2-5) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKKHKKHKA EWRSSYEDYA DKPLEKPLKL VLKVGGSEVT ELSGSGHDSS
60 70 80 90 100
YYDDRSDHER ERHKEKKKKK KKKSEKEKHL DDEERRKRKE EKKRKREREH
110 120 130 140 150
CDTEGEADDF DPGKKVEVEP PPDRPVRACR TQPAENESTP IQQLLEHFLR
160 170 180 190 200
QLQRKDPHGF FAFPVTDAIA PGYSMIIKHP MDFGTMKDKI VANEYKSVTE
210 220 230 240 250
FKADFKLMCD NAMTYNRPDT VYYKLAKKIL HAGFKMMSKQ AALLGNEDTA
260 270 280 290 300
VEEPVPEVVP VQVETAKKSK KPSREVISCM FEPEGNACSL TDSTAEEHVL
310 320 330 340 350
ALVEHAADEA RDRINRFLPG GKMGYLKRNG DGSLLYSVVN TAEPDADEEE
360 370 380 390 400
THPVDLSSLS SKLLPGFTTL GFKDERRNKV TFLSSATTAL SMQNNSVFGD
410 420 430 440 450
LKSDEMELLY SAYGDETGVQ CALSLQEFVK DAGSYSKKVV DDLLDQITGG
460 470 480 490 500
DHSRTLFQLK QRRNVPMKPP DEAKVGDTLG DSSSSVLEFM SMKSYPDVSV
510 520 530 540 550
DISMLSSLGK VKKELDPDDS HLNLDETTKL LQDLHEAQAE RGGSRPSSNL
560 570 580 590
SSLSNASERD QHHLGSPSRL SVGEQPDVTH DPYEFLQSPE PAASAKT
Length:597
Mass (Da):67,000
Last modified:June 16, 2009 - v2
Checksum:i3B248FA97948266E
GO
Isoform 2 (identifier: Q9H8M2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-391: Missing.

Note: No experimental confirmation available.
Show »
Length:206
Mass (Da):22,534
Checksum:i05D72FAD8109A693
GO
Isoform 3 (identifier: Q9H8M2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-116: Missing.
     117-133: EVEPPPDRPVRACRTQP → MKGYQSLVFNFFFLKLS

Note: No experimental confirmation available.
Show »
Length:481
Mass (Da):53,249
Checksum:i4961C172226405D2
GO
Isoform 4 (identifier: Q9H8M2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-116: Missing.
     117-133: EVEPPPDRPVRACRTQP → MKGYQSLVFNFFFLKLS
     323-349: MGYLKRNGDGSLLYSVVNTAEPDADEE → VVLCGHRERVPRGPRLSVCLSFWVGAV
     350-597: Missing.

Note: No experimental confirmation available.
Show »
Length:233
Mass (Da):26,247
Checksum:i7043139E52BD6B40
GO
Isoform 5 (identifier: Q9H8M2-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-116: Missing.
     117-133: EVEPPPDRPVRACRTQP → MKGYQSLVFNFFFLKLS
     239-239: K → KERLLALKRSMSFMQDMDFSQ

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:501
Mass (Da):55,665
Checksum:i5C68F53097BA073C
GO
Isoform 6 (identifier: Q9H8M2-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-116: Missing.
     117-155: EVEPPPDRPV...EHFLRQLQRK → MMTGQTMSER...SWNTSSASFR

Note: No experimental confirmation available.
Show »
Length:544
Mass (Da):60,763
Checksum:i00E885F57A1EC36B
GO

Sequence cautioni

The sequence BAB15565 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti146E → D in BAB14907 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_059143170A → T. Corresponds to variant dbSNP:rs34292369Ensembl.1
Natural variantiVAR_033635266A → T. Corresponds to variant dbSNP:rs34292369Ensembl.1
Natural variantiVAR_033636389A → T. Corresponds to variant dbSNP:rs414349Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0191121 – 391Missing in isoform 2. 1 PublicationAdd BLAST391
Alternative sequenceiVSP_0374931 – 116Missing in isoform 3, isoform 4, isoform 5 and isoform 6. 3 PublicationsAdd BLAST116
Alternative sequenceiVSP_043234117 – 155EVEPP…QLQRK → MMTGQTMSERGTKKRKRRRR RSPRRRSIWTMRKEGSERKR RSGSERGSTVTRRERLTTLI LGRRWRWSRPQIGQSERAGH SQPKMRAHLFSNSWNTSSAS FR in isoform 6. 1 PublicationAdd BLAST39
Alternative sequenceiVSP_037494117 – 133EVEPP…CRTQP → MKGYQSLVFNFFFLKLS in isoform 3, isoform 4 and isoform 5. 3 PublicationsAdd BLAST17
Alternative sequenceiVSP_019113239K → KERLLALKRSMSFMQDMDFS Q in isoform 5. 1 Publication1
Alternative sequenceiVSP_019114323 – 349MGYLK…DADEE → VVLCGHRERVPRGPRLSVCL SFWVGAV in isoform 4. 1 PublicationAdd BLAST27
Alternative sequenceiVSP_019115350 – 597Missing in isoform 4. 1 PublicationAdd BLAST248

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358630 mRNA Translation: AAQ88993.1
AK023503 mRNA Translation: BAB14591.1
AK024392 mRNA Translation: BAB14907.1
AK026830 mRNA Translation: BAB15565.1 Different initiation.
AK297573 mRNA Translation: BAG59964.1
AK299157 mRNA Translation: BAG61205.1
AC122719 Genomic DNA No translation available.
CH471102 Genomic DNA Translation: EAX08192.1
BC041590 mRNA Translation: AAH41590.1
DQ248311 mRNA Translation: ABB55266.1
CCDSiCCDS34127.2 [Q9H8M2-5]
CCDS34128.2 [Q9H8M2-6]
RefSeqiNP_001009877.2, NM_001009877.2 [Q9H8M2-6]
NP_001304880.1, NM_001317951.1 [Q9H8M2-1]
NP_076413.3, NM_023924.4 [Q9H8M2-5]
UniGeneiHs.449278

Genome annotation databases

EnsembliENST00000467963; ENSP00000419765; ENSG00000028310 [Q9H8M2-5]
ENST00000483173; ENSP00000419845; ENSG00000028310 [Q9H8M2-6]
GeneIDi65980
KEGGihsa:65980
UCSCiuc003jbq.4 human [Q9H8M2-5]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiBRD9_HUMAN
AccessioniPrimary (citable) accession number: Q9H8M2
Secondary accession number(s): A6NFY8
, B4DMQ2, B4DR93, Q2XUS1, Q6UWU9, Q8IUS4, Q9H5Q5, Q9H7R9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 16, 2009
Last modified: June 20, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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