UniProtKB - Q9H867 (MT21D_HUMAN)
Protein
Protein-lysine methyltransferase METTL21D
Gene
VCPKMT
Organism
Homo sapiens (Human)
Status
Functioni
Protein-lysine N-methyltransferase that specifically trimethylates 'Lys-315' of VCP/p97; this modification may decrease VCP ATPase activity.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 43 | S-adenosyl-L-methionine; via amide nitrogen1 Publication | 1 | |
Binding sitei | 96 | S-adenosyl-L-methionine1 Publication | 1 | |
Binding sitei | 126 | S-adenosyl-L-methionine; via amide nitrogen1 Publication | 1 | |
Binding sitei | 143 | S-adenosyl-L-methionine; via carbonyl oxygen1 Publication | 1 | |
Binding sitei | 148 | S-adenosyl-L-methionine1 Publication | 1 |
GO - Molecular functioni
- ATPase binding Source: UniProtKB
- protein-lysine N-methyltransferase activity Source: UniProtKB
GO - Biological processi
- negative regulation of ATPase activity Source: UniProtKB
- peptidyl-lysine methylation Source: UniProtKB
- peptidyl-lysine trimethylation Source: UniProtKB
- protein methylation Source: Reactome
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
PathwayCommonsi | Q9H867 |
Reactomei | R-HSA-8876725, Protein methylation |
SIGNORi | Q9H867 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein-lysine methyltransferase METTL21D (EC:2.1.1.-)Alternative name(s): Methyltransferase-like protein 21D VCP lysine methyltransferase Short name: VCP-KMT Valosin-containing protein lysine methyltransferase |
Gene namesi | Name:VCPKMT Synonyms:C14orf138, METTL21D |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:20352, VCPKMT |
MIMi | 615260, gene |
neXtProti | NX_Q9H867 |
VEuPathDBi | HostDB:ENSG00000100483.13 |
Subcellular locationi
Other locations
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: HPA
Other locations
- cytoplasm Source: UniProtKB
- protein-containing complex Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 73 | E → Q: Loss of methyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 96 | D → A or V: Loss of methyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 144 | D → V: Loss of methyltransferase activity. 1 Publication | 1 |
Organism-specific databases
OpenTargetsi | ENSG00000100483 |
PharmGKBi | PA134866014 |
Miscellaneous databases
Pharosi | Q9H867, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3588742 |
Genetic variation databases
BioMutai | VCPKMT |
DMDMi | 152031572 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000089937 | 2 – 229 | Protein-lysine methyltransferase METTL21DAdd BLAST | 228 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 8 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9H867 |
MassIVEi | Q9H867 |
PaxDbi | Q9H867 |
PeptideAtlasi | Q9H867 |
PRIDEi | Q9H867 |
ProteomicsDBi | 81183 [Q9H867-4] 81184 [Q9H867-1] 81185 [Q9H867-2] 81186 [Q9H867-3] 81187 [Q9H867-5] |
PTM databases
iPTMneti | Q9H867 |
PhosphoSitePlusi | Q9H867 |
Expressioni
Gene expression databases
Bgeei | ENSG00000100483, Expressed in endothelial cell and 228 other tissues |
Genevisiblei | Q9H867, HS |
Organism-specific databases
HPAi | ENSG00000100483, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with ALKBH6.
Interacts with ASPSCR1 and UBXN6; interaction with ASPSCR1, but not with UBXN6, enhances VCP methylation.
2 PublicationsBinary interactionsi
Q9H867
With | #Exp. | IntAct |
---|---|---|
CARD9 - isoform 2 [Q9H257-2] | 3 | EBI-18393784,EBI-11530605 |
GO - Molecular functioni
- ATPase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 122744, 16 interactors |
IntActi | Q9H867, 3 interactors |
STRINGi | 9606.ENSP00000379201 |
Miscellaneous databases
RNActi | Q9H867, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9H867 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 75 – 77 | S-adenosyl-L-methionine binding1 Publication | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2793, Eukaryota |
GeneTreei | ENSGT00940000157135 |
HOGENOMi | CLU_1890393_0_0_1 |
InParanoidi | Q9H867 |
OMAi | KAMVLNW |
OrthoDBi | 1494909at2759 |
PhylomeDBi | Q9H867 |
TreeFami | TF352990 |
Family and domain databases
InterProi | View protein in InterPro IPR019410, Methyltransf_16 IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR14614, PTHR14614, 1 hit |
Pfami | View protein in Pfam PF10294, Methyltransf_16, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
s (5)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketIsoform 4 (identifier: Q9H867-4) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MADTLESSLE DPLRSFVRVL EKRDGTVLRL QQYSSGGVGC VVWDAAIVLS
60 70 80 90 100
KYLETPEFSG DGAHALSRRS VLELGSGTGA VGLMAATLGA DVVVTDLEEL
110 120 130 140 150
QDLLKMNINM NKHLVTGSVQ AKVLKWGEEI EGFPSPPDFI LMADCIYYEE
160 170 180 190 200
SLEPLLKTLK DISGFETCII CCYEQRTMGK NPEIEKKYFE LLQLDFDFEK
210 220
IPLEKHDEEY RSEDIHIIYI RKKKSKFPS
Sequence cautioni
The sequence CAD62329 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_059621 | 63 | A → D. Corresponds to variant dbSNP:rs11157729Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008814 | 1 – 89 | MADTL…AATLG → MWGRLLQAFPPVPTPQSTLF YR in isoform 2. 1 PublicationAdd BLAST | 89 | |
Alternative sequenceiVSP_008815 | 90 – 229 | Missing in isoform 3. 1 PublicationAdd BLAST | 140 | |
Alternative sequenceiVSP_026586 | 123 – 144 | VLKWG…ILMAD → GGRNRRLSFSTRLHTDGRLH IL in isoform 1. 2 PublicationsAdd BLAST | 22 | |
Alternative sequenceiVSP_026587 | 145 – 229 | Missing in isoform 1. 2 PublicationsAdd BLAST | 85 | |
Alternative sequenceiVSP_008816 | 191 – 229 | Missing in isoform 2. 1 PublicationAdd BLAST | 39 | |
Alternative sequenceiVSP_043258 | 191 – 225 | Missing in isoform 5. 1 PublicationAdd BLAST | 35 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK023982 mRNA Translation: BAB14752.1 AK129564 mRNA Translation: BAC85183.1 BX247997 mRNA Translation: CAD62329.1 Different initiation. AL109758 Genomic DNA No translation available. BC027585 mRNA No translation available. BC105118 mRNA Translation: AAI05119.1 BC143673 mRNA Translation: AAI43674.1 BC143674 mRNA Translation: AAI43675.1 |
CCDSi | CCDS41951.1 [Q9H867-5] CCDS9696.2 [Q9H867-4] |
RefSeqi | NP_001035752.1, NM_001040662.1 [Q9H867-5] NP_078834.2, NM_024558.2 [Q9H867-4] |
Genome annotation databases
Ensembli | ENST00000395859; ENSP00000379200; ENSG00000100483 [Q9H867-5] ENST00000395860; ENSP00000379201; ENSG00000100483 [Q9H867-4] ENST00000491402; ENSP00000437113; ENSG00000100483 [Q9H867-1] |
GeneIDi | 79609 |
KEGGi | hsa:79609 |
UCSCi | uc001wxo.2, human [Q9H867-4] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK023982 mRNA Translation: BAB14752.1 AK129564 mRNA Translation: BAC85183.1 BX247997 mRNA Translation: CAD62329.1 Different initiation. AL109758 Genomic DNA No translation available. BC027585 mRNA No translation available. BC105118 mRNA Translation: AAI05119.1 BC143673 mRNA Translation: AAI43674.1 BC143674 mRNA Translation: AAI43675.1 |
CCDSi | CCDS41951.1 [Q9H867-5] CCDS9696.2 [Q9H867-4] |
RefSeqi | NP_001035752.1, NM_001040662.1 [Q9H867-5] NP_078834.2, NM_024558.2 [Q9H867-4] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4LG1 | X-ray | 1.80 | A/B/C | 7-229 | [»] | |
SMRi | Q9H867 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 122744, 16 interactors |
IntActi | Q9H867, 3 interactors |
STRINGi | 9606.ENSP00000379201 |
Chemistry databases
ChEMBLi | CHEMBL3588742 |
PTM databases
iPTMneti | Q9H867 |
PhosphoSitePlusi | Q9H867 |
Genetic variation databases
BioMutai | VCPKMT |
DMDMi | 152031572 |
Proteomic databases
EPDi | Q9H867 |
MassIVEi | Q9H867 |
PaxDbi | Q9H867 |
PeptideAtlasi | Q9H867 |
PRIDEi | Q9H867 |
ProteomicsDBi | 81183 [Q9H867-4] 81184 [Q9H867-1] 81185 [Q9H867-2] 81186 [Q9H867-3] 81187 [Q9H867-5] |
Protocols and materials databases
Antibodypediai | 10369, 21 antibodies |
Genome annotation databases
Ensembli | ENST00000395859; ENSP00000379200; ENSG00000100483 [Q9H867-5] ENST00000395860; ENSP00000379201; ENSG00000100483 [Q9H867-4] ENST00000491402; ENSP00000437113; ENSG00000100483 [Q9H867-1] |
GeneIDi | 79609 |
KEGGi | hsa:79609 |
UCSCi | uc001wxo.2, human [Q9H867-4] |
Organism-specific databases
CTDi | 79609 |
GeneCardsi | VCPKMT |
HGNCi | HGNC:20352, VCPKMT |
HPAi | ENSG00000100483, Low tissue specificity |
MIMi | 615260, gene |
neXtProti | NX_Q9H867 |
OpenTargetsi | ENSG00000100483 |
PharmGKBi | PA134866014 |
VEuPathDBi | HostDB:ENSG00000100483.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2793, Eukaryota |
GeneTreei | ENSGT00940000157135 |
HOGENOMi | CLU_1890393_0_0_1 |
InParanoidi | Q9H867 |
OMAi | KAMVLNW |
OrthoDBi | 1494909at2759 |
PhylomeDBi | Q9H867 |
TreeFami | TF352990 |
Enzyme and pathway databases
PathwayCommonsi | Q9H867 |
Reactomei | R-HSA-8876725, Protein methylation |
SIGNORi | Q9H867 |
Miscellaneous databases
BioGRID-ORCSi | 79609, 15 hits in 857 CRISPR screens |
ChiTaRSi | VCPKMT, human |
GenomeRNAii | 79609 |
Pharosi | Q9H867, Tbio |
PROi | PR:Q9H867 |
RNActi | Q9H867, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000100483, Expressed in endothelial cell and 228 other tissues |
Genevisiblei | Q9H867, HS |
Family and domain databases
InterProi | View protein in InterPro IPR019410, Methyltransf_16 IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR14614, PTHR14614, 1 hit |
Pfami | View protein in Pfam PF10294, Methyltransf_16, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MT21D_HUMAN | |
Accessioni | Q9H867Primary (citable) accession number: Q9H867 Secondary accession number(s): B7ZLA3 Q86T12 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 7, 2003 |
Last sequence update: | July 10, 2007 | |
Last modified: | February 10, 2021 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human entries with genetic variants
List of human entries with genetic variants