Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphorylated adapter RNA export protein

Gene

PHAX

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner (By similarity). Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • nuclear export Source: Reactome
  • protein transport Source: UniProtKB-KW
  • snRNA export from nucleus Source: GO_Central
  • snRNA transcription by RNA polymerase II Source: Reactome

Keywordsi

Molecular functionRNA-binding
Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-191859 snRNP Assembly
R-HSA-6807505 RNA polymerase II transcribes snRNA genes

Protein family/group databases

TCDBi9.A.60.1.1 the small nuclear rna exporter (snrna-e)

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphorylated adapter RNA export protein
Alternative name(s):
RNA U small nuclear RNA export adapter protein
Gene namesi
Name:PHAX
Synonyms:RNUXA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000164902.13
HGNCiHGNC:10241 PHAX
MIMi604924 gene
neXtProtiNX_Q9H814

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000164902
PharmGKBiPA164724444

Polymorphism and mutation databases

BioMutaiPHAX
DMDMi74752718

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002397752 – 394Phosphorylated adapter RNA export proteinAdd BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei14PhosphoserineCombined sources1
Modified residuei16PhosphoserineCombined sources1
Modified residuei65PhosphoserineBy similarity1
Modified residuei66PhosphoserineBy similarity1
Modified residuei69PhosphoserineBy similarity1
Modified residuei73PhosphoserineBy similarity1
Modified residuei226PhosphoserineBy similarity1
Modified residuei296PhosphothreonineCombined sources1
Modified residuei356PhosphoserineCombined sources1
Modified residuei368PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated in the nucleus. Dephosphorylated in the cytoplasm (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9H814
MaxQBiQ9H814
PaxDbiQ9H814
PeptideAtlasiQ9H814
PRIDEiQ9H814
ProteomicsDBi81168

PTM databases

iPTMnetiQ9H814
PhosphoSitePlusiQ9H814

Miscellaneous databases

PMAP-CutDBiQ9H814

Expressioni

Gene expression databases

BgeeiENSG00000164902 Expressed in 202 organ(s), highest expression level in amniotic fluid
CleanExiHS_PHAX
GenevisibleiQ9H814 HS

Organism-specific databases

HPAiHPA059630
HPA070326

Interactioni

Subunit structurei

Found in a U snRNA export complex with PHAX/RNUXA, NCBP1/CBP80, NCBP2/CBP20, RAN, XPO1 and m7G-capped RNA. Part of a precomplex with PHAX/RNUXA, NCBP1/CBP80, NCBP2/CBP20 and m7G-capped RNA. Interacts with NCBP1/CBP80 (By similarity). Found in a complex with snoRNA. Interacts with NCBP2/CBP20 (PubMed:26382858).By similarity2 Publications

Protein-protein interaction databases

BioGridi119734, 55 interactors
CORUMiQ9H814
IntActiQ9H814, 37 interactors
MINTiQ9H814
STRINGi9606.ENSP00000297540

Structurei

Secondary structure

1394
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9H814
SMRiQ9H814
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 329Necessary for interaction with CBP80By similarityAdd BLAST328
Regioni228 – 328Sufficient for poly U RNA-bindingAdd BLAST101
Regioni279 – 287Necessary for poly U RNA-binding and snRNA exportBy similarity9

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi81 – 84Nuclear localization signalBy similarity4
Motifi130 – 139Nuclear export signalBy similarity10
Motifi198 – 201Nuclear localization signalBy similarity4

Sequence similaritiesi

Belongs to the PHAX family.Curated

Phylogenomic databases

eggNOGiKOG3948 Eukaryota
ENOG4110D9C LUCA
GeneTreeiENSGT00390000011084
HOGENOMiHOG000115525
HOVERGENiHBG058976
InParanoidiQ9H814
KOiK14291
OMAiKRESQEH
OrthoDBiEOG091G0XKP
PhylomeDBiQ9H814
TreeFamiTF321050

Family and domain databases

Gene3Di1.10.10.1440, 1 hit
InterProiView protein in InterPro
IPR039047 PHAX
IPR019385 PHAX_RNA-binding_domain
IPR038092 PHAX_RNA-binding_sf
PANTHERiPTHR13135 PTHR13135, 1 hit
PfamiView protein in Pfam
PF10258 RNA_GG_bind, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9H814-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALEVGDMED GQLSDSDSDM TVAPSDRPLQ LPKVLGGDSA MRAFQNTATA
60 70 80 90 100
CAPVSHYRAV ESVDSSEESF SDSDDDSCLW KRKRQKCFNP PPKPEPFQFG
110 120 130 140 150
QSSQKPPVAG GKKINNIWGA VLQEQNQDAV ATELGILGME GTIDRSRQSE
160 170 180 190 200
TYNYLLAKKL RKESQEHTKD LDKELDEYMH GGKKMGSKEE ENGQGHLKRK
210 220 230 240 250
RPVKDRLGNR PEMNYKGRYE ITAEDSQEKV ADEISFRLQE PKKDLIARVV
260 270 280 290 300
RIIGNKKAIE LLMETAEVEQ NGGLFIMNGS RRRTPGGVFL NLLKNTPSIS
310 320 330 340 350
EEQIKDIFYI ENQKEYENKK AARKRRTQVL GKKMKQAIKS LNFQEDDDTS
360 370 380 390
RETFASDTNE ALASLDESQE GHAEAKLEAE EAIEVDHSHD LDIF
Length:394
Mass (Da):44,403
Last modified:March 1, 2001 - v1
Checksum:i3513FA5081C6F7CA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti141G → A in BAB14489 (PubMed:14702039).Curated1
Sequence conflicti346D → A in BAB14489 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05187182R → C. Corresponds to variant dbSNP:rs3734173Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023255 mRNA Translation: BAB14489.1
AK024065 mRNA Translation: BAB14809.1
CR457305 mRNA Translation: CAG33586.1
BC021161 mRNA Translation: AAH21161.1
CCDSiCCDS4138.1
RefSeqiNP_115553.2, NM_032177.3
UniGeneiHs.555731
Hs.744042

Genome annotation databases

EnsembliENST00000297540; ENSP00000297540; ENSG00000164902
GeneIDi51808
KEGGihsa:51808
UCSCiuc003kua.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023255 mRNA Translation: BAB14489.1
AK024065 mRNA Translation: BAB14809.1
CR457305 mRNA Translation: CAG33586.1
BC021161 mRNA Translation: AAH21161.1
CCDSiCCDS4138.1
RefSeqiNP_115553.2, NM_032177.3
UniGeneiHs.555731
Hs.744042

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XC7NMR-A223-323[»]
ProteinModelPortaliQ9H814
SMRiQ9H814
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119734, 55 interactors
CORUMiQ9H814
IntActiQ9H814, 37 interactors
MINTiQ9H814
STRINGi9606.ENSP00000297540

Protein family/group databases

TCDBi9.A.60.1.1 the small nuclear rna exporter (snrna-e)

PTM databases

iPTMnetiQ9H814
PhosphoSitePlusiQ9H814

Polymorphism and mutation databases

BioMutaiPHAX
DMDMi74752718

Proteomic databases

EPDiQ9H814
MaxQBiQ9H814
PaxDbiQ9H814
PeptideAtlasiQ9H814
PRIDEiQ9H814
ProteomicsDBi81168

Protocols and materials databases

DNASUi51808
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000297540; ENSP00000297540; ENSG00000164902
GeneIDi51808
KEGGihsa:51808
UCSCiuc003kua.3 human

Organism-specific databases

CTDi51808
EuPathDBiHostDB:ENSG00000164902.13
GeneCardsiPHAX
HGNCiHGNC:10241 PHAX
HPAiHPA059630
HPA070326
MIMi604924 gene
neXtProtiNX_Q9H814
OpenTargetsiENSG00000164902
PharmGKBiPA164724444
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3948 Eukaryota
ENOG4110D9C LUCA
GeneTreeiENSGT00390000011084
HOGENOMiHOG000115525
HOVERGENiHBG058976
InParanoidiQ9H814
KOiK14291
OMAiKRESQEH
OrthoDBiEOG091G0XKP
PhylomeDBiQ9H814
TreeFamiTF321050

Enzyme and pathway databases

ReactomeiR-HSA-191859 snRNP Assembly
R-HSA-6807505 RNA polymerase II transcribes snRNA genes

Miscellaneous databases

ChiTaRSiPHAX human
GeneWikiiRNUXA
GenomeRNAii51808
PMAP-CutDBiQ9H814
PROiPR:Q9H814
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000164902 Expressed in 202 organ(s), highest expression level in amniotic fluid
CleanExiHS_PHAX
GenevisibleiQ9H814 HS

Family and domain databases

Gene3Di1.10.10.1440, 1 hit
InterProiView protein in InterPro
IPR039047 PHAX
IPR019385 PHAX_RNA-binding_domain
IPR038092 PHAX_RNA-binding_sf
PANTHERiPTHR13135 PTHR13135, 1 hit
PfamiView protein in Pfam
PF10258 RNA_GG_bind, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPHAX_HUMAN
AccessioniPrimary (citable) accession number: Q9H814
Secondary accession number(s): Q9H8W1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: March 1, 2001
Last modified: November 7, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again