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Protein

Prostaglandin E synthase 2

Gene

PTGES2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Isomerase that catalyzes the conversion of PGH2 into the more stable prostaglandin E2 (PGE2).2 Publications

Caution

Heme and GST are not required for prostaglandin synthase activity, but the protein copurifies with heme and GST when DTT is omitted during the purification procedure. The GSH-heme complex-bound enzyme (mPGES-2h) has been proposed to act as a lyase and catalyze the degradation of prostaglandin E2 H2 (PGH2) to 12(S)-hydroxy-5(Z),8(E),10(E)-heptadecatrienoic acid (HHT) and malondialdehyde (MDA), but this activity may well be spurious. According to PubMed:18198127, boiling the enzyme leads to loss of prostaglandin synthase activity, but does not eliminate the lyase activity. Besides, free heme can catalyze the formation of 12L-hydroxy-5,8,10-heptadecatrienoic acid (HHT).Curated1 Publication

Catalytic activityi

(5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-11-alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate.2 Publications

Activity regulationi

Isomerase activity is increased by sulfhydril compounds. Dithiothreitol (DTT) is most effective, followed by dihydrolipoic acid, glutathione (GSH) and 2-mercaptoethanol.1 Publication

Pathwayi: prostaglandin biosynthesis

This protein is involved in the pathway prostaglandin biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway prostaglandin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei148Glutathione; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism

Enzyme and pathway databases

BioCyciMetaCyc:HS07514-MONOMER
BRENDAi5.3.99.3 2681
ReactomeiR-HSA-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
R-HSA-6798695 Neutrophil degranulation
UniPathwayi
UPA00662

Chemistry databases

SwissLipidsiSLP:000001095

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin E synthase 2
Alternative name(s):
Membrane-associated prostaglandin E synthase-21 Publication
Short name:
mPGE synthase-21 Publication
Microsomal prostaglandin E synthase 2
Short name:
mPGES-2
Prostaglandin-H(2) E-isomerase (EC:5.3.99.32 Publications)
Cleaved into the following chain:
Gene namesi
Name:PTGES2
Synonyms:C9orf15, PGES2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000148334.14
HGNCiHGNC:17822 PTGES2
MIMi608152 gene
neXtProtiNX_Q9H7Z7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 57LumenalSequence analysisAdd BLAST57
Transmembranei58 – 74HelicalSequence analysisAdd BLAST17
Topological domaini75 – 377CytoplasmicSequence analysisAdd BLAST303

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi110C → S: Loss of function. 1 Publication1
Mutagenesisi113C → S: Slightly decreased enzyme activity. 1 Publication1

Organism-specific databases

DisGeNETi80142
OpenTargetsiENSG00000148334
PharmGKBiPA33949

Chemistry databases

ChEMBLiCHEMBL4411
GuidetoPHARMACOLOGYi1378

Polymorphism and mutation databases

BioMutaiPTGES2
DMDMi73921741

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000131271 – 377Prostaglandin E synthase 2Add BLAST377
ChainiPRO_000001312888 – 377Prostaglandin E synthase 2 truncated formBy similarityAdd BLAST290

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei95PhosphoserineCombined sources1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei87 – 88CleavageBy similarity2

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9H7Z7
MaxQBiQ9H7Z7
PaxDbiQ9H7Z7
PeptideAtlasiQ9H7Z7
PRIDEiQ9H7Z7
ProteomicsDBi81164
TopDownProteomicsiQ9H7Z7

PTM databases

iPTMnetiQ9H7Z7
PhosphoSitePlusiQ9H7Z7

Expressioni

Tissue specificityi

Widely expressed. Expressed in the heart, including apex, inter-ventricular septum, both atria and ventricles, but not in the aorta. Also expressed in fetal heart. Detected in various regions of the brain: cerebellum; occipital, frontal and parietal lobes. Also expressed in the lymph nodes, skeletal muscle, kidney and trachea, but not in the thymus or lung. Overexpressed in colorectal cancer.1 Publication

Gene expression databases

BgeeiENSG00000148334 Expressed in 203 organ(s), highest expression level in apex of heart
CleanExiHS_PTGES2
ExpressionAtlasiQ9H7Z7 baseline and differential
GenevisibleiQ9H7Z7 HS

Organism-specific databases

HPAiHPA020631
HPA020733

Interactioni

Subunit structurei

Homodimer. May interact with CEBPB (By similarity). Interacts with EXOSC10.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
C1orf189Q5VU693EBI-681645,EBI-10247920

Protein-protein interaction databases

BioGridi123135, 35 interactors
IntActiQ9H7Z7, 11 interactors
STRINGi9606.ENSP00000345341

Chemistry databases

BindingDBiQ9H7Z7

Structurei

3D structure databases

ProteinModelPortaliQ9H7Z7
SMRiQ9H7Z7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini90 – 193GlutaredoxinPROSITE-ProRule annotationAdd BLAST104
Domaini263 – 377GST C-terminalAdd BLAST115

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni164 – 165Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3029 Eukaryota
ENOG410XS2X LUCA
GeneTreeiENSGT00390000000224
HOGENOMiHOG000231901
HOVERGENiHBG069136
InParanoidiQ9H7Z7
KOiK05309
OMAiWFYDVKA
OrthoDBiEOG091G0AEH
PhylomeDBiQ9H7Z7
TreeFamiTF314304

Family and domain databases

CDDicd03197 GST_C_mPGES2, 1 hit
InterProiView protein in InterPro
IPR002109 Glutaredoxin
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR040079 Glutathione_S-Trfase
IPR004045 Glutathione_S-Trfase_N
IPR034334 PGES2
IPR034335 PGES2_C
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF13417 GST_N_3, 1 hit
SFLDiSFLDG01182 Prostaglandin_E_synthase_like, 1 hit
SFLDS00019 Glutathione_Transferase_(cytos, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS00195 GLUTAREDOXIN_1, 1 hit
PS51354 GLUTAREDOXIN_2, 1 hit
PS50405 GST_CTER, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q9H7Z7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDPAARVVRA LWPGGCALAW RLGGRPQPLL PTQSRAGFAG AAGGPSPVAA
60 70 80 90 100
ARKGSPRLLG AAALALGGAL GLYHTARWHL RAQDLHAERS AAQLSLSSRL
110 120 130 140 150
QLTLYQYKTC PFCSKVRAFL DFHALPYQVV EVNPVRRAEI KFSSYRKVPI
160 170 180 190 200
LVAQEGESSQ QLNDSSVIIS ALKTYLVSGQ PLEEIITYYP AMKAVNEQGK
210 220 230 240 250
EVTEFGNKYW LMLNEKEAQQ VYGGKEARTE EMKWRQWADD WLVHLISPNV
260 270 280 290 300
YRTPTEALAS FDYIVREGKF GAVEGAVAKY MGAAAMYLIS KRLKSRHRLQ
310 320 330 340 350
DNVREDLYEA ADKWVAAVGK DRPFMGGQKP NLADLAVYGV LRVMEGLDAF
360 370
DDLMQHTHIQ PWYLRVERAI TEASPAH
Length:377
Mass (Da):41,943
Last modified:March 1, 2001 - v1
Checksum:i82F2DF867BB8337C
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A6NHH0A6NHH0_HUMAN
Prostaglandin E synthase 2, isoform...
PTGES2 hCG_1785478
186Annotation score:
X6RJ95X6RJ95_HUMAN
Prostaglandin E synthase 2
PTGES2
265Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti319G → S in BAD97240 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049494298R → H. Corresponds to variant dbSNP:rs13283456Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024100 mRNA Translation: BAB14826.1
AK223520 mRNA Translation: BAD97240.1
AL590708 Genomic DNA No translation available.
BC009397 mRNA Translation: AAH09397.2
BC009456 mRNA Translation: AAH09456.1
BC011613 mRNA Translation: AAH11613.1
CCDSiCCDS6891.1
RefSeqiNP_001243264.1, NM_001256335.1
NP_079348.1, NM_025072.6
NP_945176.1, NM_198938.2
UniGeneiHs.495219

Genome annotation databases

EnsembliENST00000338961; ENSP00000345341; ENSG00000148334
GeneIDi80142
KEGGihsa:80142
UCSCiuc004bti.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024100 mRNA Translation: BAB14826.1
AK223520 mRNA Translation: BAD97240.1
AL590708 Genomic DNA No translation available.
BC009397 mRNA Translation: AAH09397.2
BC009456 mRNA Translation: AAH09456.1
BC011613 mRNA Translation: AAH11613.1
CCDSiCCDS6891.1
RefSeqiNP_001243264.1, NM_001256335.1
NP_079348.1, NM_025072.6
NP_945176.1, NM_198938.2
UniGeneiHs.495219

3D structure databases

ProteinModelPortaliQ9H7Z7
SMRiQ9H7Z7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123135, 35 interactors
IntActiQ9H7Z7, 11 interactors
STRINGi9606.ENSP00000345341

Chemistry databases

BindingDBiQ9H7Z7
ChEMBLiCHEMBL4411
GuidetoPHARMACOLOGYi1378
SwissLipidsiSLP:000001095

PTM databases

iPTMnetiQ9H7Z7
PhosphoSitePlusiQ9H7Z7

Polymorphism and mutation databases

BioMutaiPTGES2
DMDMi73921741

Proteomic databases

EPDiQ9H7Z7
MaxQBiQ9H7Z7
PaxDbiQ9H7Z7
PeptideAtlasiQ9H7Z7
PRIDEiQ9H7Z7
ProteomicsDBi81164
TopDownProteomicsiQ9H7Z7

Protocols and materials databases

DNASUi80142
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000338961; ENSP00000345341; ENSG00000148334
GeneIDi80142
KEGGihsa:80142
UCSCiuc004bti.4 human

Organism-specific databases

CTDi80142
DisGeNETi80142
EuPathDBiHostDB:ENSG00000148334.14
GeneCardsiPTGES2
HGNCiHGNC:17822 PTGES2
HPAiHPA020631
HPA020733
MIMi608152 gene
neXtProtiNX_Q9H7Z7
OpenTargetsiENSG00000148334
PharmGKBiPA33949
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3029 Eukaryota
ENOG410XS2X LUCA
GeneTreeiENSGT00390000000224
HOGENOMiHOG000231901
HOVERGENiHBG069136
InParanoidiQ9H7Z7
KOiK05309
OMAiWFYDVKA
OrthoDBiEOG091G0AEH
PhylomeDBiQ9H7Z7
TreeFamiTF314304

Enzyme and pathway databases

UniPathwayi
UPA00662

BioCyciMetaCyc:HS07514-MONOMER
BRENDAi5.3.99.3 2681
ReactomeiR-HSA-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
R-HSA-6798695 Neutrophil degranulation

Miscellaneous databases

ChiTaRSiPTGES2 human
GeneWikiiPTGES2
GenomeRNAii80142
PROiPR:Q9H7Z7
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000148334 Expressed in 203 organ(s), highest expression level in apex of heart
CleanExiHS_PTGES2
ExpressionAtlasiQ9H7Z7 baseline and differential
GenevisibleiQ9H7Z7 HS

Family and domain databases

CDDicd03197 GST_C_mPGES2, 1 hit
InterProiView protein in InterPro
IPR002109 Glutaredoxin
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR040079 Glutathione_S-Trfase
IPR004045 Glutathione_S-Trfase_N
IPR034334 PGES2
IPR034335 PGES2_C
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF13417 GST_N_3, 1 hit
SFLDiSFLDG01182 Prostaglandin_E_synthase_like, 1 hit
SFLDS00019 Glutathione_Transferase_(cytos, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS00195 GLUTAREDOXIN_1, 1 hit
PS51354 GLUTAREDOXIN_2, 1 hit
PS50405 GST_CTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPGES2_HUMAN
AccessioniPrimary (citable) accession number: Q9H7Z7
Secondary accession number(s): Q53EW9
, Q5SYV6, Q96GI0, Q96GL2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: March 1, 2001
Last modified: November 7, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
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