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Protein

Histone acetyltransferase KAT8

Gene

KAT8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Histone acetyltransferase which may be involved in transcriptional activation. May influence the function of ATM. As part of the MSL complex it is involved in acetylation of nucleosomal histone H4 producing specifically H4K16ac. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. That activity is less specific than the one of the MSL complex. Can also acetylate TP53/p53 at 'Lys-120'.6 Publications

Catalytic activityi

Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine.4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei350Proton donor/acceptor2 PublicationsCurated1
Binding sitei354Acetyl-CoA2 Publications1
Binding sitei363Acetyl-CoA2 Publications1
Binding sitei432Acetyl-CoA2 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri207 – 232C2HC MYST-typePROSITE-ProRule annotation1 PublicationAdd BLAST26

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Acyltransferase, Chromatin regulator, Transferase
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi2.3.1.48 2681
ReactomeiR-HSA-3214847 HATs acetylate histones
SIGNORiQ9H7Z6

Names & Taxonomyi

Protein namesi
Recommended name:
Histone acetyltransferase KAT8 (EC:2.3.1.484 Publications)
Alternative name(s):
Lysine acetyltransferase 8
MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1
Short name:
MYST-1
Short name:
hMOF
Gene namesi
Name:KAT8
Synonyms:MOF, MYST1
ORF Names:PP7073
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000103510.19
HGNCiHGNC:17933 KAT8
MIMi609912 gene
neXtProtiNX_Q9H7Z6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi274K → A: Abolishes histone acetyltransferase activity. 1 Publication1
Mutagenesisi274K → R: Abolishes histone acetyltransferase activity. 1 Publication1
Mutagenesisi316C → S: Strongly reduces histone acetyltransferase activity. 1 Publication1
Mutagenesisi350E → Q: Abolishes histone acetyltransferase activity. 1 Publication1

Organism-specific databases

DisGeNETi84148
OpenTargetsiENSG00000103510
PharmGKBiPA38476

Chemistry databases

ChEMBLiCHEMBL1932912
GuidetoPHARMACOLOGYi2668

Polymorphism and mutation databases

BioMutaiKAT8
DMDMi68565938

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000515662 – 458Histone acetyltransferase KAT8Add BLAST457

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei37PhosphoserineBy similarity1
Modified residuei42PhosphoserineBy similarity1
Modified residuei113N6-acetyllysineCombined sources1
Modified residuei274N6-acetyllysine; by autocatalysis5 Publications1
Modified residuei348PhosphoserineCombined sources1

Post-translational modificationi

Autoacetylation at Lys-274 is required for binding histone H4 with high affinity and for proper function.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9H7Z6
MaxQBiQ9H7Z6
PaxDbiQ9H7Z6
PeptideAtlasiQ9H7Z6
PRIDEiQ9H7Z6
ProteomicsDBi81162
81163 [Q9H7Z6-2]

PTM databases

iPTMnetiQ9H7Z6
PhosphoSitePlusiQ9H7Z6

Expressioni

Gene expression databases

BgeeiENSG00000103510 Expressed in 230 organ(s), highest expression level in cerebellar vermis
CleanExiHS_MYST1
ExpressionAtlasiQ9H7Z6 baseline and differential
GenevisibleiQ9H7Z6 HS

Organism-specific databases

HPAiHPA066324

Interactioni

Subunit structurei

Component of a multisubunit histone acetyltransferase complex (MSL) at least composed of the MOF/KAT8, MSL1/hampin, MSL2L1 and MSL3L1. Interacts with MSL1; the interaction is direct. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Interacts with KANSL1; the interaction is direct. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MOF/KAT8, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with the chromodomain of MORF4L1/MRG15. Interacts with ATM through the chromodomain. Interacts with KANSL1; the interaction is direct.9 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi123914, 43 interactors
ComplexPortaliCPX-809 NSL histone acetyltransferase complex
CPX-815 MSL histone acetyltransferase complex
CORUMiQ9H7Z6
IntActiQ9H7Z6, 12 interactors
MINTiQ9H7Z6
STRINGi9606.ENSP00000406037

Chemistry databases

BindingDBiQ9H7Z6

Structurei

Secondary structure

1458
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9H7Z6
SMRiQ9H7Z6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9H7Z6

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini69 – 123ChromoAdd BLAST55
Domaini174 – 447MYST-type HATPROSITE-ProRule annotationAdd BLAST274

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni174 – 458Sufficient for interaction with KANSL1Add BLAST285
Regioni317 – 319Acetyl-CoA binding2 Publications3
Regioni324 – 330Acetyl-CoA binding2 Publications7

Sequence similaritiesi

Belongs to the MYST (SAS/MOZ) family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri207 – 232C2HC MYST-typePROSITE-ProRule annotation1 PublicationAdd BLAST26

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2747 Eukaryota
COG5027 LUCA
GeneTreeiENSGT00920000148999
HOVERGENiHBG053268
InParanoidiQ9H7Z6
KOiK11308
OMAiMNMVKYW
OrthoDBiEOG091G0B73
PhylomeDBiQ9H7Z6
TreeFamiTF317619

Family and domain databases

CDDicd00024 CHROMO, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR016197 Chromo-like_dom_sf
IPR000953 Chromo/chromo_shadow_dom
IPR002717 HAT_MYST-type
IPR037906 KAT8
IPR025995 Tudor-knot
IPR036388 WH-like_DNA-bd_sf
PANTHERiPTHR10615:SF82 PTHR10615:SF82, 1 hit
PfamiView protein in Pfam
PF01853 MOZ_SAS, 1 hit
PF11717 Tudor-knot, 1 hit
SMARTiView protein in SMART
SM00298 CHROMO, 1 hit
SUPFAMiSSF54160 SSF54160, 1 hit
SSF55729 SSF55729, 1 hit
PROSITEiView protein in PROSITE
PS51726 MYST_HAT, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9H7Z6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAQGAAAAV AAGTSGVAGE GEPGPGENAA AEGTAPSPGR VSPPTPARGE
60 70 80 90 100
PEVTVEIGET YLCRRPDSTW HSAEVIQSRV NDQEGREEFY VHYVGFNRRL
110 120 130 140 150
DEWVDKNRLA LTKTVKDAVQ KNSEKYLSEL AEQPERKITR NQKRKHDEIN
160 170 180 190 200
HVQKTYAEMD PTTAALEKEH EAITKVKYVD KIHIGNYEID AWYFSPFPED
210 220 230 240 250
YGKQPKLWLC EYCLKYMKYE KSYRFHLGQC QWRQPPGKEI YRKSNISVYE
260 270 280 290 300
VDGKDHKIYC QNLCLLAKLF LDHKTLYFDV EPFVFYILTE VDRQGAHIVG
310 320 330 340 350
YFSKEKESPD GNNVACILTL PPYQRRGYGK FLIAFSYELS KLESTVGSPE
360 370 380 390 400
KPLSDLGKLS YRSYWSWVLL EILRDFRGTL SIKDLSQMTS ITQNDIISTL
410 420 430 440 450
QSLNMVKYWK GQHVICVTPK LVEEHLKSAQ YKKPPITVDS VCLKWAPPKH

KQVKLSKK
Length:458
Mass (Da):52,403
Last modified:July 5, 2005 - v2
Checksum:i66C474BE5B90E8E3
GO
Isoform 2 (identifier: Q9H7Z6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     438-458: VDSVCLKWAPPKHKQVKLSKK → GGWGAAVCRGRWGSVSIWTGRSQGLLIAVT

Show »
Length:467
Mass (Da):53,085
Checksum:i69AA4F8800E687E0
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BMX5H3BMX5_HUMAN
Histone acetyltransferase
KAT8
187Annotation score:

Sequence cautioni

The sequence AAL55762 differs from that shown. Probable cloning artifact.Curated
The sequence AAL56648 differs from that shown. Reason: Frameshift at positions 23, 26 and 52.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti222S → T in AAL55762 (PubMed:15498874).Curated1
Sequence conflicti249Y → H in BAB14827 (PubMed:14702039).Curated1
Sequence conflicti372I → N in BAB14827 (PubMed:14702039).Curated1
Isoform 2 (identifier: Q9H7Z6-2)
Sequence conflicti454I → M in AAH37773 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014579438 – 458VDSVC…KLSKK → GGWGAAVCRGRWGSVSIWTG RSQGLLIAVT in isoform 2. 2 PublicationsAdd BLAST21

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK021872 mRNA Translation: BAB13924.1
AK024102 mRNA Translation: BAB14827.1
AK291106 mRNA Translation: BAF83795.1
AC009088 Genomic DNA No translation available.
AC135050 Genomic DNA No translation available.
CH471192 Genomic DNA Translation: EAW52157.1
CH471192 Genomic DNA Translation: EAW52158.1
BC037773 mRNA Translation: AAH37773.1
AF217501 mRNA Translation: AAL56648.1 Frameshift.
AF260665 mRNA Translation: AAF72665.2
AF289578 mRNA Translation: AAL55762.1 Sequence problems.
AL050395 mRNA Translation: CAH56416.1
CCDSiCCDS10706.1 [Q9H7Z6-1]
CCDS45468.1 [Q9H7Z6-2]
RefSeqiNP_115564.2, NM_032188.2 [Q9H7Z6-1]
NP_892003.2, NM_182958.2 [Q9H7Z6-2]
UniGeneiHs.533803

Genome annotation databases

EnsembliENST00000219797; ENSP00000219797; ENSG00000103510 [Q9H7Z6-1]
ENST00000448516; ENSP00000406037; ENSG00000103510 [Q9H7Z6-2]
ENST00000543774; ENSP00000456933; ENSG00000103510 [Q9H7Z6-1]
GeneIDi84148
KEGGihsa:84148
UCSCiuc002eax.4 human [Q9H7Z6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK021872 mRNA Translation: BAB13924.1
AK024102 mRNA Translation: BAB14827.1
AK291106 mRNA Translation: BAF83795.1
AC009088 Genomic DNA No translation available.
AC135050 Genomic DNA No translation available.
CH471192 Genomic DNA Translation: EAW52157.1
CH471192 Genomic DNA Translation: EAW52158.1
BC037773 mRNA Translation: AAH37773.1
AF217501 mRNA Translation: AAL56648.1 Frameshift.
AF260665 mRNA Translation: AAF72665.2
AF289578 mRNA Translation: AAL55762.1 Sequence problems.
AL050395 mRNA Translation: CAH56416.1
CCDSiCCDS10706.1 [Q9H7Z6-1]
CCDS45468.1 [Q9H7Z6-2]
RefSeqiNP_115564.2, NM_032188.2 [Q9H7Z6-1]
NP_892003.2, NM_182958.2 [Q9H7Z6-2]
UniGeneiHs.533803

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GIVX-ray1.94A174-449[»]
2PQ8X-ray1.45A174-449[»]
2Y0MX-ray2.70A174-458[»]
3QAHX-ray2.10A174-449[»]
3TOAX-ray3.00A177-458[»]
3TOBX-ray2.70A177-458[»]
4DNCX-ray2.05A/B170-458[»]
5H43X-ray2.30B140-149[»]
C128-142[»]
5J8CX-ray2.17A177-458[»]
5J8FX-ray2.60A177-458[»]
5WCIX-ray1.78A174-449[»]
6BA2X-ray1.85A174-449[»]
6BA4X-ray1.95A174-449[»]
6CT2X-ray2.13A174-449[»]
ProteinModelPortaliQ9H7Z6
SMRiQ9H7Z6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123914, 43 interactors
ComplexPortaliCPX-809 NSL histone acetyltransferase complex
CPX-815 MSL histone acetyltransferase complex
CORUMiQ9H7Z6
IntActiQ9H7Z6, 12 interactors
MINTiQ9H7Z6
STRINGi9606.ENSP00000406037

Chemistry databases

BindingDBiQ9H7Z6
ChEMBLiCHEMBL1932912
GuidetoPHARMACOLOGYi2668

PTM databases

iPTMnetiQ9H7Z6
PhosphoSitePlusiQ9H7Z6

Polymorphism and mutation databases

BioMutaiKAT8
DMDMi68565938

Proteomic databases

EPDiQ9H7Z6
MaxQBiQ9H7Z6
PaxDbiQ9H7Z6
PeptideAtlasiQ9H7Z6
PRIDEiQ9H7Z6
ProteomicsDBi81162
81163 [Q9H7Z6-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000219797; ENSP00000219797; ENSG00000103510 [Q9H7Z6-1]
ENST00000448516; ENSP00000406037; ENSG00000103510 [Q9H7Z6-2]
ENST00000543774; ENSP00000456933; ENSG00000103510 [Q9H7Z6-1]
GeneIDi84148
KEGGihsa:84148
UCSCiuc002eax.4 human [Q9H7Z6-1]

Organism-specific databases

CTDi84148
DisGeNETi84148
EuPathDBiHostDB:ENSG00000103510.19
GeneCardsiKAT8
HGNCiHGNC:17933 KAT8
HPAiHPA066324
MIMi609912 gene
neXtProtiNX_Q9H7Z6
OpenTargetsiENSG00000103510
PharmGKBiPA38476
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2747 Eukaryota
COG5027 LUCA
GeneTreeiENSGT00920000148999
HOVERGENiHBG053268
InParanoidiQ9H7Z6
KOiK11308
OMAiMNMVKYW
OrthoDBiEOG091G0B73
PhylomeDBiQ9H7Z6
TreeFamiTF317619

Enzyme and pathway databases

BRENDAi2.3.1.48 2681
ReactomeiR-HSA-3214847 HATs acetylate histones
SIGNORiQ9H7Z6

Miscellaneous databases

ChiTaRSiKAT8 human
EvolutionaryTraceiQ9H7Z6
GeneWikiiMYST1
GenomeRNAii84148
PROiPR:Q9H7Z6
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000103510 Expressed in 230 organ(s), highest expression level in cerebellar vermis
CleanExiHS_MYST1
ExpressionAtlasiQ9H7Z6 baseline and differential
GenevisibleiQ9H7Z6 HS

Family and domain databases

CDDicd00024 CHROMO, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR016197 Chromo-like_dom_sf
IPR000953 Chromo/chromo_shadow_dom
IPR002717 HAT_MYST-type
IPR037906 KAT8
IPR025995 Tudor-knot
IPR036388 WH-like_DNA-bd_sf
PANTHERiPTHR10615:SF82 PTHR10615:SF82, 1 hit
PfamiView protein in Pfam
PF01853 MOZ_SAS, 1 hit
PF11717 Tudor-knot, 1 hit
SMARTiView protein in SMART
SM00298 CHROMO, 1 hit
SUPFAMiSSF54160 SSF54160, 1 hit
SSF55729 SSF55729, 1 hit
PROSITEiView protein in PROSITE
PS51726 MYST_HAT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKAT8_HUMAN
AccessioniPrimary (citable) accession number: Q9H7Z6
Secondary accession number(s): A8K4Z1
, G5E9P2, Q659G0, Q7LC17, Q8IY59, Q8WYB4, Q8WZ14, Q9HAC5, Q9NR35
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: October 10, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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