UniProtKB - Q9H777 (RNZ1_HUMAN)
Protein
Zinc phosphodiesterase ELAC protein 1
Gene
ELAC1
Organism
Homo sapiens (Human)
Status
Functioni
Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.
Catalytic activityi
- Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.1 Publication EC:3.1.26.11
Cofactori
Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 62 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 64 | Zinc 1; catalyticBy similarity | 1 | |
Active sitei | 66 | Proton acceptorSequence analysis | 1 | |
Metal bindingi | 66 | Zinc 2; catalyticBy similarity | 1 | |
Metal bindingi | 67 | Zinc 2; catalyticBy similarity | 1 | |
Metal bindingi | 182 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 253 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 253 | Zinc 2; catalyticBy similarity | 1 | |
Metal bindingi | 313 | Zinc 2; catalyticBy similarity | 1 |
GO - Molecular functioni
- 3'-tRNA processing endoribonuclease activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
Keywordsi
Molecular function | Endonuclease, Hydrolase, Nuclease |
Biological process | tRNA processing |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.1.26.11, 2681 |
PathwayCommonsi | Q9H777 |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc phosphodiesterase ELAC protein 1 (EC:3.1.26.11)Alternative name(s): Deleted in Ma29 ElaC homolog protein 1 Ribonuclease Z 1 Short name: RNase Z 1 tRNA 3 endonuclease 1 tRNase Z 1 |
Gene namesi | Name:ELAC1 Synonyms:D29 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:14197, ELAC1 |
MIMi | 608079, gene |
neXtProti | NX_Q9H777 |
VEuPathDBi | HostDB:ENSG00000141642.8 |
Subcellular locationi
Cytosol
- cytosol Source: UniProtKB
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 55520 |
OpenTargetsi | ENSG00000141642 |
PharmGKBi | PA27738 |
Miscellaneous databases
Pharosi | Q9H777, Tbio |
Genetic variation databases
BioMutai | ELAC1 |
DMDMi | 41017799 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000155825 | 1 – 363 | Zinc phosphodiesterase ELAC protein 1Add BLAST | 363 |
Proteomic databases
EPDi | Q9H777 |
jPOSTi | Q9H777 |
MassIVEi | Q9H777 |
MaxQBi | Q9H777 |
PaxDbi | Q9H777 |
PeptideAtlasi | Q9H777 |
PRIDEi | Q9H777 |
ProteomicsDBi | 81086 |
PTM databases
iPTMneti | Q9H777 |
PhosphoSitePlusi | Q9H777 |
Expressioni
Tissue specificityi
Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.1 Publication
Gene expression databases
Bgeei | ENSG00000141642, Expressed in amniotic fluid and 177 other tissues |
ExpressionAtlasi | Q9H777, baseline and differential |
Genevisiblei | Q9H777, HS |
Organism-specific databases
HPAi | ENSG00000141642, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
BioGRIDi | 120694, 3 interactors |
STRINGi | 9606.ENSP00000269466 |
Miscellaneous databases
RNActi | Q9H777, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9H777 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the RNase Z family.Curated
Phylogenomic databases
eggNOGi | KOG2121, Eukaryota |
GeneTreei | ENSGT00730000111224 |
HOGENOMi | CLU_031317_2_2_1 |
InParanoidi | Q9H777 |
OMAi | GTQRQMM |
OrthoDBi | 1387038at2759 |
PhylomeDBi | Q9H777 |
TreeFami | TF324462 |
Family and domain databases
CDDi | cd07717, RNaseZ_ZiPD-like_MBL-fold, 1 hit |
HAMAPi | MF_01818, RNase_Z_BN, 1 hit |
InterProi | View protein in InterPro IPR001279, Metallo-B-lactamas IPR036866, RibonucZ/Hydroxyglut_hydro IPR013471, RNase_Z/BN |
Pfami | View protein in Pfam PF00753, Lactamase_B, 1 hit PF12706, Lactamase_B_2, 1 hit |
SUPFAMi | SSF56281, SSF56281, 1 hit |
TIGRFAMsi | TIGR02651, RNase_Z, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9H777-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSMDVTFLGT GAAYPSPTRG ASAVVLRCEG ECWLFDCGEG TQTQLMKSQL
60 70 80 90 100
KAGRITKIFI THLHGDHFFG LPGLLCTISL QSGSMVSKQP IEIYGPVGLR
110 120 130 140 150
DFIWRTMELS HTELVFHYVV HELVPTADQC PAEELKEFAH VNRADSPPKE
160 170 180 190 200
EQGRTILLDS EENSYLLFDD EQFVVKAFRL FHRIPSFGFS VVEKKRPGKL
210 220 230 240 250
NAQKLKDLGV PPGPAYGKLK NGISVVLENG VTISPQDVLK KPIVGRKICI
260 270 280 290 300
LGDCSGVVGD GGVKLCFEAD LLIHEATLDD AQMDKAKEHG HSTPQMAATF
310 320 330 340 350
AKLCRAKRLV LTHFSQRYKP VALAREGETD GIAELKKQAE SVLDLQEVTL
360
AEDFMVISIP IKK
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7EIJ1 | K7EIJ1_HUMAN | Zinc phosphodiesterase ELAC protein... | ELAC1 | 223 | Annotation score: | ||
K7EPH7 | K7EPH7_HUMAN | ElaC homolog 1 (E. coli), isoform C... | ELAC1 hCG_23690 | 88 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 314 | F → S in BAB15021 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 358 | S → G in BAB15021 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_017424 | 355 | M → V1 PublicationCorresponds to variant dbSNP:rs34524743Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB029151 mRNA Translation: BAA96799.1 AF308695 mRNA Translation: AAG24917.1 AK024822 mRNA Translation: BAB15021.1 BC014624 mRNA Translation: AAH14624.1 |
CCDSi | CCDS11949.1 |
RefSeqi | NP_061166.1, NM_018696.2 |
Genome annotation databases
Ensembli | ENST00000269466; ENSP00000269466; ENSG00000141642 |
GeneIDi | 55520 |
KEGGi | hsa:55520 |
UCSCi | uc002lez.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB029151 mRNA Translation: BAA96799.1 AF308695 mRNA Translation: AAG24917.1 AK024822 mRNA Translation: BAB15021.1 BC014624 mRNA Translation: AAH14624.1 |
CCDSi | CCDS11949.1 |
RefSeqi | NP_061166.1, NM_018696.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3ZWF | X-ray | 1.70 | A/B | 3-363 | [»] | |
SMRi | Q9H777 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 120694, 3 interactors |
STRINGi | 9606.ENSP00000269466 |
PTM databases
iPTMneti | Q9H777 |
PhosphoSitePlusi | Q9H777 |
Genetic variation databases
BioMutai | ELAC1 |
DMDMi | 41017799 |
Proteomic databases
EPDi | Q9H777 |
jPOSTi | Q9H777 |
MassIVEi | Q9H777 |
MaxQBi | Q9H777 |
PaxDbi | Q9H777 |
PeptideAtlasi | Q9H777 |
PRIDEi | Q9H777 |
ProteomicsDBi | 81086 |
Protocols and materials databases
Antibodypediai | 22721, 236 antibodies |
DNASUi | 55520 |
Genome annotation databases
Ensembli | ENST00000269466; ENSP00000269466; ENSG00000141642 |
GeneIDi | 55520 |
KEGGi | hsa:55520 |
UCSCi | uc002lez.4, human |
Organism-specific databases
CTDi | 55520 |
DisGeNETi | 55520 |
GeneCardsi | ELAC1 |
HGNCi | HGNC:14197, ELAC1 |
HPAi | ENSG00000141642, Low tissue specificity |
MIMi | 608079, gene |
neXtProti | NX_Q9H777 |
OpenTargetsi | ENSG00000141642 |
PharmGKBi | PA27738 |
VEuPathDBi | HostDB:ENSG00000141642.8 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2121, Eukaryota |
GeneTreei | ENSGT00730000111224 |
HOGENOMi | CLU_031317_2_2_1 |
InParanoidi | Q9H777 |
OMAi | GTQRQMM |
OrthoDBi | 1387038at2759 |
PhylomeDBi | Q9H777 |
TreeFami | TF324462 |
Enzyme and pathway databases
BRENDAi | 3.1.26.11, 2681 |
PathwayCommonsi | Q9H777 |
Miscellaneous databases
BioGRID-ORCSi | 55520, 2 hits in 997 CRISPR screens |
ChiTaRSi | ELAC1, human |
GeneWikii | ELAC1 |
GenomeRNAii | 55520 |
Pharosi | Q9H777, Tbio |
PROi | PR:Q9H777 |
RNActi | Q9H777, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000141642, Expressed in amniotic fluid and 177 other tissues |
ExpressionAtlasi | Q9H777, baseline and differential |
Genevisiblei | Q9H777, HS |
Family and domain databases
CDDi | cd07717, RNaseZ_ZiPD-like_MBL-fold, 1 hit |
HAMAPi | MF_01818, RNase_Z_BN, 1 hit |
InterProi | View protein in InterPro IPR001279, Metallo-B-lactamas IPR036866, RibonucZ/Hydroxyglut_hydro IPR013471, RNase_Z/BN |
Pfami | View protein in Pfam PF00753, Lactamase_B, 1 hit PF12706, Lactamase_B_2, 1 hit |
SUPFAMi | SSF56281, SSF56281, 1 hit |
TIGRFAMsi | TIGR02651, RNase_Z, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RNZ1_HUMAN | |
Accessioni | Q9H777Primary (citable) accession number: Q9H777 Secondary accession number(s): Q9NS99 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 16, 2004 |
Last sequence update: | January 16, 2004 | |
Last modified: | April 7, 2021 | |
This is version 154 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 18
Human chromosome 18: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families