UniProtKB - Q9H714 (PACER_HUMAN)
Protein associated with UVRAG as autophagy enhancer
RUBCNL
Functioni
GO - Molecular functioni
- phosphatidylinositol-3-phosphate binding Source: UniProtKB
- phosphatidylinositol-4-phosphate binding Source: UniProtKB
- phosphatidylinositol-5-phosphate binding Source: UniProtKB
- phosphatidylinositol phosphate binding Source: GO_Central
GO - Biological processi
- autophagosome-endosome fusion Source: UniProtKB
- autophagosome-lysosome fusion Source: UniProtKB
- autophagosome maturation Source: UniProtKB
- lipid metabolic process Source: UniProtKB-KW
- regulation of glycogen metabolic process Source: UniProtKB
- regulation of lipid metabolic process Source: UniProtKB
Keywordsi
Biological process | Autophagy, Lipid metabolism |
Ligand | Lipid-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9H714 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein associated with UVRAG as autophagy enhancer1 PublicationShort name: Pacer1 Publication Alternative name(s): Protein Rubicon-likeCurated |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:20420, RUBCNL |
neXtProti | NX_Q9H714 |
VEuPathDBi | HostDB:ENSG00000102445.18 |
Subcellular locationi
Other locations
- autophagosome membrane 1 Publication; Peripheral membrane protein 1 Publication
Note: Associates with late autophagic structure (PubMed:28306502). Recruitment to autophagosome membrane is promoted by autophagic stimuli (PubMed:28306502).1 Publication
Other locations
- autophagosome membrane Source: UniProtKB
- cytoplasmic vesicle Source: UniProtKB-KW
- intracellular membrane-bounded organelle Source: GO_Central
Keywords - Cellular componenti
Cytoplasmic vesicle, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 157 | S → A: Abolished phosphorylation by MTOR, leading to promote interaction with STX17 and autophagosome maturation. 1 Publication | 1 | |
Mutagenesisi | 157 | S → D: Phosphomimetic mutant; impaired interaction with STX17 and abolished ability to promote autophagosome maturation. 1 Publication | 1 | |
Mutagenesisi | 204 – 208 | VEKEN → AAAAA: Abolishes interaction with UVRAG. 1 Publication | 5 | |
Mutagenesisi | 483 | K → R: Abolished acetylation by KAT5/TIP60 and abolished ability to promote autophagosome maturation; when associated with R-523, R-533, R-573 and R-633. | 1 | |
Mutagenesisi | 523 | K → R: Abolished acetylation by KAT5/TIP60 and abolished ability to promote autophagosome maturation; when associated with R-483, R-533, R-573 and R-633. | 1 | |
Mutagenesisi | 533 | K → R: Abolished acetylation by KAT5/TIP60 and abolished ability to promote autophagosome maturation; when associated with R-483, R-523, R-573 and R-633. | 1 | |
Mutagenesisi | 573 | K → R: Abolished acetylation by KAT5/TIP60 and abolished ability to promote autophagosome maturation; when associated with R-483, R-523, R-533 and R-633. | 1 | |
Mutagenesisi | 633 | K → R: Abolished acetylation by KAT5/TIP60 and abolished ability to promote autophagosome maturation; when associated with R-483, R-523, R-533 and R-573. | 1 |
Organism-specific databases
DisGeNETi | 80183 |
OpenTargetsi | ENSG00000102445 |
PharmGKBi | PA134942431 |
Miscellaneous databases
Pharosi | Q9H714, Tbio |
Genetic variation databases
BioMutai | RUBCNL |
DMDMi | 206729926 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000089880 | 1 – 662 | Protein associated with UVRAG as autophagy enhancerAdd BLAST | 662 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 157 | Phosphoserine; by MTOR1 Publication | 1 | |
Modified residuei | 483 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 523 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 533 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 573 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 633 | N6-acetyllysine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | Q9H714 |
MassIVEi | Q9H714 |
PaxDbi | Q9H714 |
PeptideAtlasi | Q9H714 |
PRIDEi | Q9H714 |
ProteomicsDBi | 1901 81072 [Q9H714-5] 81073 [Q9H714-1] 81074 [Q9H714-2] 81075 [Q9H714-3] 81076 [Q9H714-4] |
PTM databases
iPTMneti | Q9H714 |
PhosphoSitePlusi | Q9H714 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000102445, Expressed in bone marrow and 197 other tissues |
ExpressionAtlasi | Q9H714, baseline and differential |
Genevisiblei | Q9H714, HS |
Organism-specific databases
HPAi | ENSG00000102445, Tissue enhanced (blood, lymphoid tissue) |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsIsoform 2 [Q9H714-3]
With | #Exp. | IntAct |
---|---|---|
BECN1 [Q14457] | 3 | EBI-9088146,EBI-949378 |
RUFY1 - isoform 2 [Q96T51-2] | 2 | EBI-9088146,EBI-12192715 |
Protein-protein interaction databases
BioGRIDi | 123162, 10 interactors |
IntActi | Q9H714, 9 interactors |
STRINGi | 9606.ENSP00000374558 |
Miscellaneous databases
RNActi | Q9H714, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 196 – 235 | Interaction with UVRAG1 PublicationAdd BLAST | 40 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 86 – 131 | Ser-richAdd BLAST | 46 |
Phylogenomic databases
eggNOGi | KOG1829, Eukaryota |
GeneTreei | ENSGT00940000160585 |
InParanoidi | Q9H714 |
OMAi | FEQVPSH |
OrthoDBi | 177737at2759 |
PhylomeDBi | Q9H714 |
TreeFami | TF317067 |
Family and domain databases
InterProi | View protein in InterPro IPR025258, Zf-RING_9 |
Pfami | View protein in Pfam PF13901, zf-RING_9, 1 hit |
SMARTi | View protein in SMART SM01175, DUF4206, 1 hit |
s (6+)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVSQSTVRQD SPVEPWEGIS DHSGIIDGSP RLLNTDHPPC QLDIRLMRHK
60 70 80 90 100
AVWINPQDVQ QQPQDLQSQV PAAGNSGTHF VTDAASPSGP SPSCLGDSLA
110 120 130 140 150
ETTLSEDTTD SVGSASPHGS SEKSSSFSLS STEVHMVRPG YSHRVSLPTS
160 170 180 190 200
PGILATSPYP ETDSAFFEPS HLTSAADEGA VQVSRRTISS NSFSPEVFVL
210 220 230 240 250
PVDVEKENAH FYVADMIISA MEKMKCNILS QQQTESWSKE VSGLLGSDQP
260 270 280 290 300
DSEMTFDTNI KQESGSSTSS YSGYEGCAVL QVSPVTETRT YHDVKEICKC
310 320 330 340 350
DVDEFVILEL GDFNDITETC SCSCSSSKSV TYEPDFNSAE LLAKELYRVF
360 370 380 390 400
QKCWILSVVN SQLAGSLSAA GSIVVNEECV RKDFESSMNV VQEIKFKSRI
410 420 430 440 450
RGTEDWAPPR FQIIFNIHPP LKRDLVVAAQ NFFCAGCGTP VEPKFVKRLR
460 470 480 490 500
YCEYLGKYFC DCCHSYAESC IPARILMMWD FKKYYVSNFS KQLLDSIWHQ
510 520 530 540 550
PIFNLLSIGQ SLYAKAKELD RVKEIQEQLF HIKKLLKTCR FANSALKEFE
560 570 580 590 600
QVPGHLTDEL HLFSLEDLVR IKKGLLAPLL KDILKASLAH VAGCELCQGK
610 620 630 640 650
GFICEFCQNT TVIFPFQTAT CRRCSACRAC FHKQCFQSSE CPRCARITAR
660
RKLLESVASA AT
The sequence of this isoform differs from the canonical sequence as follows:
309-321: ELGDFNDITETCS → VSLMSQTSILQNY
322-662: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
136-147: MVRPGYSHRVSL → KACMRKPRSWTE
148-662: Missing.
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA8XR14 | A8XR14_HUMAN | Protein-associated with UVRAG as au... | RUBCNL | 178 | Annotation score: | ||
Q5W050 | Q5W050_HUMAN | Protein-associated with UVRAG as au... | RUBCNL | 129 | Annotation score: | ||
E7EMA2 | E7EMA2_HUMAN | Protein-associated with UVRAG as au... | RUBCNL | 207 | Annotation score: | ||
A0A6Q8PFX0 | A0A6Q8PFX0_HUMAN | Protein-associated with UVRAG as au... | RUBCNL | 571 | Annotation score: | ||
A0A6Q8PF39 | A0A6Q8PF39_HUMAN | Protein-associated with UVRAG as au... | RUBCNL | 479 | Annotation score: | ||
A0A6Q8PH05 | A0A6Q8PH05_HUMAN | Protein-associated with UVRAG as au... | RUBCNL | 253 | Annotation score: | ||
A0A0A0MRV7 | A0A0A0MRV7_HUMAN | Protein-associated with UVRAG as au... | RUBCNL | 447 | Annotation score: | ||
B7ZBN5 | B7ZBN5_HUMAN | Protein-associated with UVRAG as au... | RUBCNL | 505 | Annotation score: | ||
A0A6Q8PGJ9 | A0A6Q8PGJ9_HUMAN | Protein-associated with UVRAG as au... | RUBCNL | 105 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 234 – 236 | TES → ARG in AAH04495 (PubMed:15489334).Curated | 3 | |
Sequence conflicti | 469 | S → P in BAG52649 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 549 | F → L in BAG52649 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_022912 | 152 | G → R2 PublicationsCorresponds to variant dbSNP:rs1408184Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_055260 | 1 – 135 | Missing in isoform 6. 1 PublicationAdd BLAST | 135 | |
Alternative sequenceiVSP_014708 | 1 – 88 | MVSQS…AASPS → MVSNHYFLLCVNLPLREIHT P in isoform 2. 1 PublicationAdd BLAST | 88 | |
Alternative sequenceiVSP_014709 | 136 – 147 | MVRPG…HRVSL → KACMRKPRSWTE in isoform 4. 1 PublicationAdd BLAST | 12 | |
Alternative sequenceiVSP_014710 | 148 – 662 | Missing in isoform 4. 1 PublicationAdd BLAST | 515 | |
Alternative sequenceiVSP_014711 | 309 – 321 | ELGDF…TETCS → VSLMSQTSILQNY in isoform 3. 1 PublicationAdd BLAST | 13 | |
Alternative sequenceiVSP_014712 | 322 – 662 | Missing in isoform 3. 1 PublicationAdd BLAST | 341 | |
Alternative sequenceiVSP_014713 | 545 – 662 | ALKEF…ASAAT → CVKERALFVNFARIRLSSSH FRQQHVEDVQRAGLAFTNSA SSPPSAPGVRGSQRGENFWK VWPLQQHDAPEYCEKDCSTC LMITPICVYYW in isoform 5. 1 PublicationAdd BLAST | 118 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK025215 mRNA Translation: BAB15086.1 AK093073 mRNA Translation: BAG52649.1 AK293034 mRNA Translation: BAF85723.1 AL139801 Genomic DNA No translation available. BC004495 mRNA Translation: AAH04495.1 BC021097 mRNA No translation available. BC032311 mRNA No translation available. BC043488 mRNA Translation: AAH43488.1 |
CCDSi | CCDS31970.2 [Q9H714-5] CCDS66542.1 [Q9H714-6] CCDS66543.1 [Q9H714-4] CCDS66545.1 [Q9H714-3] |
RefSeqi | NP_001273690.1, NM_001286761.1 [Q9H714-5] NP_001273691.1, NM_001286762.1 [Q9H714-4] NP_001273692.1, NM_001286763.1 [Q9H714-3] NP_001273693.1, NM_001286764.1 [Q9H714-6] NP_079389.2, NM_025113.3 [Q9H714-5] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK025215 mRNA Translation: BAB15086.1 AK093073 mRNA Translation: BAG52649.1 AK293034 mRNA Translation: BAF85723.1 AL139801 Genomic DNA No translation available. BC004495 mRNA Translation: AAH04495.1 BC021097 mRNA No translation available. BC032311 mRNA No translation available. BC043488 mRNA Translation: AAH43488.1 |
CCDSi | CCDS31970.2 [Q9H714-5] CCDS66542.1 [Q9H714-6] CCDS66543.1 [Q9H714-4] CCDS66545.1 [Q9H714-3] |
RefSeqi | NP_001273690.1, NM_001286761.1 [Q9H714-5] NP_001273691.1, NM_001286762.1 [Q9H714-4] NP_001273692.1, NM_001286763.1 [Q9H714-3] NP_001273693.1, NM_001286764.1 [Q9H714-6] NP_079389.2, NM_025113.3 [Q9H714-5] |
3D structure databases
SMRi | Q9H714 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 123162, 10 interactors |
IntActi | Q9H714, 9 interactors |
STRINGi | 9606.ENSP00000374558 |
PTM databases
iPTMneti | Q9H714 |
PhosphoSitePlusi | Q9H714 |
Genetic variation databases
BioMutai | RUBCNL |
DMDMi | 206729926 |
Proteomic databases
jPOSTi | Q9H714 |
MassIVEi | Q9H714 |
PaxDbi | Q9H714 |
PeptideAtlasi | Q9H714 |
PRIDEi | Q9H714 |
ProteomicsDBi | 1901 81072 [Q9H714-5] 81073 [Q9H714-1] 81074 [Q9H714-2] 81075 [Q9H714-3] 81076 [Q9H714-4] |
Protocols and materials databases
Antibodypediai | 23706, 144 antibodies |
Genome annotation databases
Organism-specific databases
CTDi | 80183 |
DisGeNETi | 80183 |
GeneCardsi | RUBCNL |
HGNCi | HGNC:20420, RUBCNL |
HPAi | ENSG00000102445, Tissue enhanced (blood, lymphoid tissue) |
neXtProti | NX_Q9H714 |
OpenTargetsi | ENSG00000102445 |
PharmGKBi | PA134942431 |
VEuPathDBi | HostDB:ENSG00000102445.18 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1829, Eukaryota |
GeneTreei | ENSGT00940000160585 |
InParanoidi | Q9H714 |
OMAi | FEQVPSH |
OrthoDBi | 177737at2759 |
PhylomeDBi | Q9H714 |
TreeFami | TF317067 |
Enzyme and pathway databases
PathwayCommonsi | Q9H714 |
Miscellaneous databases
BioGRID-ORCSi | 80183, 3 hits in 989 CRISPR screens |
ChiTaRSi | RUBCNL, human |
GenomeRNAii | 80183 |
Pharosi | Q9H714, Tbio |
PROi | PR:Q9H714 |
RNActi | Q9H714, protein |
Gene expression databases
Bgeei | ENSG00000102445, Expressed in bone marrow and 197 other tissues |
ExpressionAtlasi | Q9H714, baseline and differential |
Genevisiblei | Q9H714, HS |
Family and domain databases
InterProi | View protein in InterPro IPR025258, Zf-RING_9 |
Pfami | View protein in Pfam PF13901, zf-RING_9, 1 hit |
SMARTi | View protein in SMART SM01175, DUF4206, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PACER_HUMAN | |
Accessioni | Q9H714Primary (citable) accession number: Q9H714 Secondary accession number(s): A8KAG9 Q8N5J6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | September 23, 2008 | |
Last modified: | April 7, 2021 | |
This is version 134 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 13
Human chromosome 13: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports