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Protein

Palmitoyltransferase ZDHHC6

Gene

ZDHHC6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endoplasmic reticulum palmitoyl acyltransferase that mediates palmitoylation of proteins such as AMFR, CALX, ITPR1 and TFRC (PubMed:22314232, PubMed:22728137, PubMed:25368151, PubMed:28826475). Palmitoylates calnexin (CALX), which is required for its association with the ribosome-translocon complex and efficient folding of glycosylated proteins (PubMed:22314232). Mediates palmitoylation of AMFR, promoting AMFR distribution to the peripheral endoplasmic reticulum (PubMed:22728137). Together with SELENOK, palmitoylates ITPR1 in immune cells, leading to regulate ITPR1 stability and function (PubMed:25368151).4 Publications

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei129S-palmitoyl cysteine intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Palmitoyltransferase ZDHHC6Curated (EC:2.3.1.2251 Publication)
Alternative name(s):
Transmembrane protein H41 Publication
Zinc finger DHHC domain-containing protein 61 Publication
Short name:
DHHC-61 Publication
Zinc finger protein 376
Gene namesi
Name:ZDHHC6Imported
Synonyms:ZNF376Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000023041.11
HGNCiHGNC:19160 ZDHHC6
neXtProtiNX_Q9H6R6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei25 – 45HelicalSequence analysisAdd BLAST21
Transmembranei58 – 78HelicalSequence analysisAdd BLAST21
Transmembranei144 – 164HelicalSequence analysisAdd BLAST21
Transmembranei206 – 226HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi328 – 329CC → AA: Abolishes palmitoylation, leading to impaired homooligomeization and decreased catalytic activity; when associated with A-343. 1 Publication2
Mutagenesisi343C → A: Abolishes palmitoylation, leading to impaired homooligomeization and decreased catalytic activity; when associated with 328-A-A-329. 1 Publication1
Mutagenesisi409E → A: Does not affect localization to the endoplasmic reticulum. 1 Publication1
Mutagenesisi410K → A: Impaired localization to the endoplasmic reticulum. 1 Publication1
Mutagenesisi411K → A: Impaired localization to the endoplasmic reticulum. 1 Publication1
Mutagenesisi412N → A: Does not affect localization to the endoplasmic reticulum. 1 Publication1
Mutagenesisi413R → A: Does not affect localization to the endoplasmic reticulum. 1 Publication1

Organism-specific databases

DisGeNETi64429
OpenTargetsiENSG00000023041
PharmGKBiPA38803

Polymorphism and mutation databases

BioMutaiZDHHC6
DMDMi28202106

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002128711 – 413Palmitoyltransferase ZDHHC6Add BLAST413

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi328S-palmitoyl cysteine1 Publication1
Lipidationi329S-palmitoyl cysteine1 Publication1
Lipidationi343S-palmitoyl cysteine1 Publication1

Post-translational modificationi

Palmitoylated at 3 different sites by ZDHHC16 (PubMed:28826475). The combination of the different palmitoylation events strongly affects the quaternary assembly of ZDHHC6, its localization, stability and function (PubMed:28826475). Palmitoylation at Cys-328 accelerates the turnover of ZDHHC6 (PubMed:28826475). Depalmitoylated by LYPLA2 (PubMed:28826475).1 Publication

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

EPDiQ9H6R6
MaxQBiQ9H6R6
PaxDbiQ9H6R6
PeptideAtlasiQ9H6R6
PRIDEiQ9H6R6
ProteomicsDBi81020
81021 [Q9H6R6-2]

PTM databases

iPTMnetiQ9H6R6
PhosphoSitePlusiQ9H6R6
SwissPalmiQ9H6R6

Expressioni

Gene expression databases

BgeeiENSG00000023041 Expressed in 221 organ(s), highest expression level in metanephric glomerulus
CleanExiHS_ZDHHC6
ExpressionAtlasiQ9H6R6 baseline and differential
GenevisibleiQ9H6R6 HS

Organism-specific databases

HPAiHPA014357

Interactioni

Subunit structurei

Homooligomerizes (PubMed:28826475). Interacts with SELENOK (PubMed:25368151).2 Publications

Protein-protein interaction databases

BioGridi122177, 24 interactors
IntActiQ9H6R6, 1 interactor
STRINGi9606.ENSP00000358413

Structurei

3D structure databases

ProteinModelPortaliQ9H6R6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini99 – 149DHHCPROSITE-ProRule annotationAdd BLAST51
Domaini313 – 398SH3PROSITE-ProRule annotationAdd BLAST86

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi410 – 413Di-lysine motif1 Publication4

Domaini

The DHHC domain is required for palmitoyltransferase activity.By similarity
The C-terminal di-lysine motif confers endoplasmic reticulum localization.1 Publication

Sequence similaritiesi

Belongs to the DHHC palmitoyltransferase family.Curated

Keywords - Domaini

SH3 domain, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1314 Eukaryota
COG5273 LUCA
GeneTreeiENSGT00900000140969
HOGENOMiHOG000017896
HOVERGENiHBG054221
InParanoidiQ9H6R6
KOiK20031
OMAiWFPRRCV
OrthoDBiEOG091G0HOK
PhylomeDBiQ9H6R6
TreeFamiTF320809

Family and domain databases

InterProiView protein in InterPro
IPR001594 Palmitoyltrfase_DHHC
IPR001452 SH3_domain
PfamiView protein in Pfam
PF01529 DHHC, 1 hit
PF07653 SH3_2, 1 hit
PROSITEiView protein in PROSITE
PS50216 DHHC, 1 hit
PS50002 SH3, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.iShow all

Isoform 1 (identifier: Q9H6R6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGTFCSVIKF ENLQELKRLC HWGPIIALGV IAICSTMAMI DSVLWYWPLH
60 70 80 90 100
TTGGSVNFIM LINWTVMILY NYFNAMFVGP GFVPLGWKPE ISQDTMYLQY
110 120 130 140 150
CKVCQAYKAP RSHHCRKCNR CVMKMDHHCP WINNCCGYQN HASFTLFLLL
160 170 180 190 200
APLGCIHAAF IFVMTMYTQL YHRLSFGWNT VKIDMSAARR DPLPIVPFGL
210 220 230 240 250
AAFATTLFAL GLALGTTIAV GMLFFIQMKI ILRNKTSIES WIEEKAKDRI
260 270 280 290 300
QYYQLDEVFV FPYDMGSRWR NFKQVFTWSG VPEGDGLEWP VREGCHQYSL
310 320 330 340 350
TIEQLKQKAD KRVRSVRYKV IEDYSGACCP LNKGIKTFFT SPCTEEPRIQ
360 370 380 390 400
LQKGEFILAT RGLRYWLYGD KILDDSFIEG VSRIRGWFPR KCVEKCPCDA
410
ETDQAPEGEK KNR
Length:413
Mass (Da):47,663
Last modified:March 1, 2001 - v1
Checksum:iE4C6CD875FA03713
GO
Isoform 2 (identifier: Q9H6R6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     90-93: Missing.

Note: No experimental confirmation available.
Show »
Length:409
Mass (Da):47,205
Checksum:iAC56FA6C4DBDE189
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0D9SEX5A0A0D9SEX5_HUMAN
Palmitoyltransferase
ZDHHC6
383Annotation score:

Sequence cautioni

The sequence BAB15462 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57N → S in BAD96806 (Ref. 3) Curated1
Sequence conflicti66V → I in BAD96806 (Ref. 3) Curated1
Sequence conflicti78V → A in BAD96806 (Ref. 3) Curated1
Sequence conflicti342P → L in BAB15462 (PubMed:14702039).Curated1
Sequence conflicti370D → G in BAB15462 (PubMed:14702039).Curated1
Sequence conflicti376S → P in BAD96806 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05297441D → N. Corresponds to variant dbSNP:rs34350728Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00693890 – 93Missing in isoform 2. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF267740 mRNA Translation: AAL56663.1
AK025605 mRNA Translation: BAB15187.1
AK026369 mRNA Translation: BAB15462.1 Different initiation.
AK223086 mRNA Translation: BAD96806.1
CH471066 Genomic DNA Translation: EAW49526.1
CH471066 Genomic DNA Translation: EAW49528.1
BC007213 mRNA Translation: AAH07213.1
BC017434 mRNA Translation: AAH17434.1
CCDSiCCDS7574.1 [Q9H6R6-1]
CCDS76335.1 [Q9H6R6-2]
RefSeqiNP_001290063.1, NM_001303134.1 [Q9H6R6-2]
NP_071939.1, NM_022494.2 [Q9H6R6-1]
XP_005270108.1, XM_005270051.4
XP_006718011.1, XM_006717948.3
XP_016872050.1, XM_017016561.1
XP_016872051.1, XM_017016562.1 [Q9H6R6-2]
XP_016872052.1, XM_017016563.1 [Q9H6R6-2]
UniGeneiHs.196990

Genome annotation databases

EnsembliENST00000369404; ENSP00000358412; ENSG00000023041 [Q9H6R6-2]
ENST00000369405; ENSP00000358413; ENSG00000023041 [Q9H6R6-1]
GeneIDi64429
KEGGihsa:64429
UCSCiuc001kzv.3 human [Q9H6R6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiZDHC6_HUMAN
AccessioniPrimary (citable) accession number: Q9H6R6
Secondary accession number(s): D3DRB6
, Q53G45, Q96IV7, Q9H605
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: March 1, 2001
Last modified: September 12, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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