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Protein

ATP-dependent RNA helicase DHX33

Gene

DHX33

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Implicated in nucleolar organization, ribosome biogenesis, protein synthesis and cytoplasmic dsRNA sensing (By similarity) (PubMed:21930779, PubMed:23871209, PubMed:26100019). Stimulates RNA polymerase I transcription of the 47S precursor rRNA. Associates with ribosomal DNA (rDNA) loci where it is involved in POLR1A recruitment (PubMed:21930779). In the cytoplasm, promotes elongation-competent 80S ribosome assembly at the late stage of mRNA translation initiation (PubMed:26100019). Senses cytosolic dsRNA mediating NLRP3 inflammasome formation in macrophages and type I interferon production in myeloid dendritic cells (PubMed:23871209). Required for NLRP3 activation induced by viral dsRNA and bacterial RNA (PubMed:23871209). In dendritic cells, required for induction of type I interferon production induced by cytoplasmic dsRNA via the activation of MAPK and NF-kappa-B signaling pathways (By similarity).By similarity3 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi97 – 104ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • activating transcription factor binding Source: Ensembl
  • ATP binding Source: UniProtKB-KW
  • ATP-dependent 3'-5' RNA helicase activity Source: GO_Central
  • double-stranded RNA binding Source: UniProtKB
  • mRNA binding Source: UniProtKB
  • rDNA binding Source: UniProtKB
  • ribosomal large subunit binding Source: UniProtKB
  • RNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DHX33Curated (EC:3.6.4.13)
Alternative name(s):
DEAH box protein 33
Gene namesi
Name:DHX33Imported
Synonyms:DDX33
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000005100.12
HGNCiHGNC:16718 DHX33
MIMi614405 gene
neXtProtiNX_Q9H6R0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Inflammasome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi103K → N: No effect on inflammasome activation upon dsRNA-binding. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000005100
PharmGKBiPA27220

Polymorphism and mutation databases

BioMutaiDHX33
DMDMi296434478

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551641 – 707ATP-dependent RNA helicase DHX33Add BLAST707

Post-translational modificationi

Ubiquitinated, leading to its degradation by the proteasome. Deubiquitinated by USP36.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiQ9H6R0
MaxQBiQ9H6R0
PaxDbiQ9H6R0
PeptideAtlasiQ9H6R0
PRIDEiQ9H6R0
ProteomicsDBi81012
81013 [Q9H6R0-2]

PTM databases

iPTMnetiQ9H6R0
PhosphoSitePlusiQ9H6R0

Expressioni

Gene expression databases

BgeeiENSG00000005100 Expressed in 202 organ(s), highest expression level in secondary oocyte
CleanExiHS_DHX33
ExpressionAtlasiQ9H6R0 baseline and differential
GenevisibleiQ9H6R0 HS

Organism-specific databases

HPAiHPA073875

Interactioni

Subunit structurei

Interacts with UBTF (PubMed:21930779). Interacts with DDX3X, EIF3G and EIF3H; the interaction is independent of RNA (PubMed:26100019). Interacts (via HA2 region and Helicase C-terminal domain) with the components of the large ribosomal subunit RPL3, RPL7, RPL26 and RPL27 (PubMed:26100019). Interacts (via DEAH box) with NLRP3 (via NACHT domain) (PubMed:23871209). Binds to mRNA (PubMed:26100019). Binds to double-stranded RNA (via the helicase C-terminal domain) (PubMed:23871209). Interacts (via the helicase C-terminal domain) with MAVS (By similarity).By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi121247, 22 interactors
IntActiQ9H6R0, 11 interactors
MINTiQ9H6R0
STRINGi9606.ENSP00000225296

Structurei

3D structure databases

ProteinModelPortaliQ9H6R0
SMRiQ9H6R0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini84 – 252Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST169
Domaini277 – 450Helicase C-terminalPROSITE-ProRule annotationAdd BLAST174

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 80Required for nucleolar location1 PublicationAdd BLAST80
Regioni471 – 562HA2; required for interaction with EIF3G and RPL261 PublicationAdd BLAST92

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi194 – 197DEAH box4
Motifi547 – 558Critical for rDNA-bindingBy similarityAdd BLAST12

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0922 Eukaryota
COG1643 LUCA
GeneTreeiENSGT00910000144017
HOGENOMiHOG000175261
HOVERGENiHBG039428
InParanoidiQ9H6R0
KOiK17820
OMAiMDIDHFG
OrthoDBiEOG091G030Q
PhylomeDBiQ9H6R0
TreeFamiTF354245

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR002464 DNA/RNA_helicase_DEAH_CS
IPR011709 DUF1605
IPR007502 Helicase-assoc_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF04408 HA2, 1 hit
PF00271 Helicase_C, 1 hit
PF07717 OB_NTP_bind, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00847 HA2, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00690 DEAH_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9H6R0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPEEAGFPPA KRFRPGSGPP SRAGSFPPGR QVVMLLTAGS GGRGGGGGRR
60 70 80 90 100
QQPPLAQPSA SPYPEAVELQ RRSLPIFQAR GQLLAQLRNL DNAVLIGETG
110 120 130 140 150
SGKTTQIPQY LYEGGISRQG IIAVTQPRRV AAISLATRVS DEKRTELGKL
160 170 180 190 200
VGYTVRFDDV TSEDTRIKFL TDGMLLREAI SDSLLRKYSC VILDEAHERT
210 220 230 240 250
IHTDVLFGVV KAAQKRRKEL GKLPLKVIVM SATMDVDLFS QYFNGAPVLY
260 270 280 290 300
LEGRQHPIQV FYTKQPQNDY LHAALVSVFQ IHQEAPSSQD ILVFLTGQEE
310 320 330 340 350
IEAMSKTCRD IAKHLPDGCP AMLVLPLYAS LPYAQQLRVF QGAPKGYRKV
360 370 380 390 400
IISTNIAETS ITITGIKYVV DTGMVKAKKY NPDSGLEVLA VQRVSKTQAW
410 420 430 440 450
QRTGRAGRED SGICYRLYTE DEFEKFDKMT VPEIQRCNLA SVMLQLLAMK
460 470 480 490 500
VPNVLTFDFM SKPSPDHIQA AIAQLDLLGA LEHKDDQLTL TPMGRKMAAF
510 520 530 540 550
PLEPKFAKTI LMSPKFHCTE EILTIVSLLS VDSVLHNPPS RREEVQGVRK
560 570 580 590 600
KFISSEGDHM TLLNIYRTFK NLGGNKDWCK ENFVNSKNMT LVAEVRAQLR
610 620 630 640 650
DICLKMSMPI ASSRGDVESV RRCLAHSLFM STAELQPDGT YATTDTHQPV
660 670 680 690 700
AIHPSSVLFH CKPACVVYTE LLYTNKCYMR DLCVIDAQWL YEAAPEYFRR

KLRTARN
Length:707
Mass (Da):78,874
Last modified:May 18, 2010 - v2
Checksum:iE3515B12BD972431
GO
Isoform 2 (identifier: Q9H6R0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-173: Missing.

Show »
Length:534
Mass (Da):60,393
Checksum:iA82774C3D3FC205E
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
I3L1L6I3L1L6_HUMAN
ATP-dependent RNA helicase DHX33
DHX33
617Annotation score:
Q05BE5Q05BE5_HUMAN
ATP-dependent RNA helicase DHX33
DHX33 hCG_1985693
483Annotation score:
I3L0M5I3L0M5_HUMAN
ATP-dependent RNA helicase DHX33
DHX33
93Annotation score:

Sequence cautioni

The sequence AAH30017 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77F → L in BAB15193 (PubMed:14702039).Curated1
Sequence conflicti166R → G in BAB15193 (PubMed:14702039).Curated1
Sequence conflicti377A → V in AAH30017 (PubMed:15489334).Curated1
Sequence conflicti539P → S in CAI56793 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_057239118R → C. Corresponds to variant dbSNP:rs8069315Ensembl.1
Natural variantiVAR_057240483H → D. Corresponds to variant dbSNP:rs11653658Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0162561 – 173Missing in isoform 2. 2 PublicationsAdd BLAST173

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK025625 mRNA Translation: BAB15193.1
AK026944 mRNA Translation: BAB15596.1
AK295074 mRNA Translation: BAG58120.1
AC004148 Genomic DNA No translation available.
CH471108 Genomic DNA Translation: EAW90332.1
BC030017 mRNA Translation: AAH30017.1 Different initiation.
CR936655 mRNA Translation: CAI56793.1
CCDSiCCDS11072.1 [Q9H6R0-1]
RefSeqiNP_001186628.1, NM_001199699.1 [Q9H6R0-2]
NP_064547.2, NM_020162.3 [Q9H6R0-1]
XP_016880366.1, XM_017024877.1
UniGeneiHs.250456

Genome annotation databases

EnsembliENST00000225296; ENSP00000225296; ENSG00000005100 [Q9H6R0-1]
GeneIDi56919
KEGGihsa:56919
UCSCiuc002gca.3 human [Q9H6R0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK025625 mRNA Translation: BAB15193.1
AK026944 mRNA Translation: BAB15596.1
AK295074 mRNA Translation: BAG58120.1
AC004148 Genomic DNA No translation available.
CH471108 Genomic DNA Translation: EAW90332.1
BC030017 mRNA Translation: AAH30017.1 Different initiation.
CR936655 mRNA Translation: CAI56793.1
CCDSiCCDS11072.1 [Q9H6R0-1]
RefSeqiNP_001186628.1, NM_001199699.1 [Q9H6R0-2]
NP_064547.2, NM_020162.3 [Q9H6R0-1]
XP_016880366.1, XM_017024877.1
UniGeneiHs.250456

3D structure databases

ProteinModelPortaliQ9H6R0
SMRiQ9H6R0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121247, 22 interactors
IntActiQ9H6R0, 11 interactors
MINTiQ9H6R0
STRINGi9606.ENSP00000225296

PTM databases

iPTMnetiQ9H6R0
PhosphoSitePlusiQ9H6R0

Polymorphism and mutation databases

BioMutaiDHX33
DMDMi296434478

Proteomic databases

EPDiQ9H6R0
MaxQBiQ9H6R0
PaxDbiQ9H6R0
PeptideAtlasiQ9H6R0
PRIDEiQ9H6R0
ProteomicsDBi81012
81013 [Q9H6R0-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000225296; ENSP00000225296; ENSG00000005100 [Q9H6R0-1]
GeneIDi56919
KEGGihsa:56919
UCSCiuc002gca.3 human [Q9H6R0-1]

Organism-specific databases

CTDi56919
EuPathDBiHostDB:ENSG00000005100.12
GeneCardsiDHX33
HGNCiHGNC:16718 DHX33
HPAiHPA073875
MIMi614405 gene
neXtProtiNX_Q9H6R0
OpenTargetsiENSG00000005100
PharmGKBiPA27220
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0922 Eukaryota
COG1643 LUCA
GeneTreeiENSGT00910000144017
HOGENOMiHOG000175261
HOVERGENiHBG039428
InParanoidiQ9H6R0
KOiK17820
OMAiMDIDHFG
OrthoDBiEOG091G030Q
PhylomeDBiQ9H6R0
TreeFamiTF354245

Miscellaneous databases

ChiTaRSiDHX33 human
GenomeRNAii56919
PROiPR:Q9H6R0
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000005100 Expressed in 202 organ(s), highest expression level in secondary oocyte
CleanExiHS_DHX33
ExpressionAtlasiQ9H6R0 baseline and differential
GenevisibleiQ9H6R0 HS

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR002464 DNA/RNA_helicase_DEAH_CS
IPR011709 DUF1605
IPR007502 Helicase-assoc_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF04408 HA2, 1 hit
PF00271 Helicase_C, 1 hit
PF07717 OB_NTP_bind, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00847 HA2, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00690 DEAH_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDHX33_HUMAN
AccessioniPrimary (citable) accession number: Q9H6R0
Secondary accession number(s): B4DHF9
, Q4G149, Q5CZ73, Q9H5M9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: May 18, 2010
Last modified: November 7, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
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