UniProtKB - Q9H6Q4 (CIAO3_HUMAN)
Protein
Cytosolic iron-sulfur assembly component 3
Gene
CIAO3
Organism
Homo sapiens (Human)
Status
Functioni
Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. Seems to negatively regulate the level of HIF1A expression, although this effect could be indirect.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 24 | Iron-sulfur 1 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 71 | Iron-sulfur 1 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 74 | Iron-sulfur 1 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 77 | Iron-sulfur 1 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 190 | Iron-sulfur 2 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 246 | Iron-sulfur 2 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 395 | Iron-sulfur 2 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 399 | Iron-sulfur 2 (4Fe-4S)Sequence analysis | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- hematopoietic progenitor cell differentiation Source: Ensembl
- iron-sulfur cluster assembly Source: UniProtKB
- oxygen homeostasis Source: HGNC-UCL
- regulation of gene expression Source: BHF-UCL
- response to hypoxia Source: HGNC-UCL
Keywordsi
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9H6Q4 |
Reactomei | R-HSA-2564830, Cytosolic iron-sulfur cluster assembly |
Names & Taxonomyi
Protein namesi | Recommended name: Cytosolic iron-sulfur assembly component 3CuratedAlternative name(s): Cytosolic Fe-S cluster assembly factor NARFL Iron-only hydrogenase-like protein 11 Publication Short name: IOP11 Publication Nuclear prelamin A recognition factor-like protein Protein related to Narf |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:14179, CIAO3 |
MIMi | 611118, gene |
neXtProti | NX_Q9H6Q4 |
VEuPathDBi | HostDB:ENSG00000103245.13 |
Subcellular locationi
Cytosol
- CIA complex Source: UniProtKB
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 64428 |
OpenTargetsi | ENSG00000103245 |
PharmGKBi | PA128394707 |
Miscellaneous databases
Pharosi | Q9H6Q4, Tbio |
Genetic variation databases
BioMutai | NARFL |
DMDMi | 74733617 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000288485 | 2 – 476 | Cytosolic iron-sulfur assembly component 3Add BLAST | 475 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q9H6Q4 |
jPOSTi | Q9H6Q4 |
MassIVEi | Q9H6Q4 |
MaxQBi | Q9H6Q4 |
PaxDbi | Q9H6Q4 |
PeptideAtlasi | Q9H6Q4 |
PRIDEi | Q9H6Q4 |
ProteomicsDBi | 81009 [Q9H6Q4-1] 81010 [Q9H6Q4-2] 81011 [Q9H6Q4-3] |
PTM databases
iPTMneti | Q9H6Q4 |
MetOSitei | Q9H6Q4 |
PhosphoSitePlusi | Q9H6Q4 |
Expressioni
Tissue specificityi
Widely expressed.1 Publication
Gene expression databases
Bgeei | ENSG00000103245, Expressed in right hemisphere of cerebellum and 179 other tissues |
ExpressionAtlasi | Q9H6Q4, baseline and differential |
Genevisiblei | Q9H6Q4, HS |
Organism-specific databases
HPAi | ENSG00000103245, Low tissue specificity |
Interactioni
Subunit structurei
Protein-protein interaction databases
BioGRIDi | 122176, 16 interactors |
CORUMi | Q9H6Q4 |
IntActi | Q9H6Q4, 7 interactors |
STRINGi | 9606.ENSP00000251588 |
Miscellaneous databases
RNActi | Q9H6Q4, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the NARF family.Curated
Phylogenomic databases
eggNOGi | KOG2439, Eukaryota |
GeneTreei | ENSGT00940000153514 |
HOGENOMi | CLU_018240_0_0_1 |
InParanoidi | Q9H6Q4 |
OMAi | FTWVVDT |
OrthoDBi | 705416at2759 |
PhylomeDBi | Q9H6Q4 |
TreeFami | TF106273 |
Family and domain databases
InterProi | View protein in InterPro IPR009016, Fe_hydrogenase IPR004108, Fe_hydrogenase_lsu_C IPR003149, Fe_hydrogenase_ssu |
Pfami | View protein in Pfam PF02906, Fe_hyd_lg_C, 1 hit PF02256, Fe_hyd_SSU, 1 hit |
SMARTi | View protein in SMART SM00902, Fe_hyd_SSU, 1 hit |
SUPFAMi | SSF53920, SSF53920, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9H6Q4-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASPFSGALQ LTDLDDFIGP SQECIKPVKV EKRAGSGVAK IRIEDDGSYF
60 70 80 90 100
QINQDGGTRR LEKAKVSLND CLACSGCITS AETVLITQQS HEELKKVLDA
110 120 130 140 150
NKMAAPSQQR LVVVSVSPQS RASLAARFQL NPTDTARKLT SFFKKIGVHF
160 170 180 190 200
VFDTAFSRHF SLLESQREFV RRFRGQADCR QALPLLASAC PGWICYAEKT
210 220 230 240 250
HGSFILPHIS TARSPQQVMG SLVKDFFAQQ QHLTPDKIYH VTVMPCYDKK
260 270 280 290 300
LEASRPDFFN QEHQTRDVDC VLTTGEVFRL LEEEGVSLPD LEPAPLDSLC
310 320 330 340 350
SGASAEEPTS HRGGGSGGYL EHVFRHAARE LFGIHVAEVT YKPLRNKDFQ
360 370 380 390 400
EVTLEKEGQV LLHFAMAYGF RNIQNLVQRL KRGRCPYHYV EVMACPSGCL
410 420 430 440 450
NGGGQLQAPD RPSRELLQHV ERLYGMVRAE APEDAPGVQE LYTHWLQGTD
460 470
SECAGRLLHT QYHAVEKAST GLGIRW
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB4DEE7 | B4DEE7_HUMAN | Cytosolic iron-sulfur assembly comp... | CIAO3 | 188 | Annotation score: | ||
H3BSH2 | H3BSH2_HUMAN | Cytosolic iron-sulfur assembly comp... | CIAO3 | 186 | Annotation score: | ||
I3L2A3 | I3L2A3_HUMAN | Cytosolic iron-sulfur assembly comp... | CIAO3 | 53 | Annotation score: | ||
H3BSJ7 | H3BSJ7_HUMAN | Cytosolic iron-sulfur assembly comp... | CIAO3 | 110 | Annotation score: | ||
H3BQ03 | H3BQ03_HUMAN | Cytosolic iron-sulfur assembly comp... | CIAO3 | 70 | Annotation score: | ||
H3BPX8 | H3BPX8_HUMAN | Cytosolic iron-sulfur assembly comp... | CIAO3 | 82 | Annotation score: | ||
H3BUF3 | H3BUF3_HUMAN | Cytosolic iron-sulfur assembly comp... | CIAO3 | 66 | Annotation score: |
Sequence cautioni
The sequence AAK61251 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 205 | I → T in BAD96725 (Ref. 3) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_053911 | 38 | V → M. Corresponds to variant dbSNP:rs8045850EnsemblClinVar. | 1 | |
Natural variantiVAR_053912 | 444 | H → R. Corresponds to variant dbSNP:rs7188554Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_025694 | 1 – 243 | Missing in isoform 2. 1 PublicationAdd BLAST | 243 | |
Alternative sequenceiVSP_025695 | 1 – 102 | Missing in isoform 3. 1 PublicationAdd BLAST | 102 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY129231 mRNA Translation: AAM98737.1 AK025641 mRNA Translation: BAB15199.1 AK025861 mRNA Translation: BAB15261.1 AK095675 mRNA Translation: BAG53105.1 AK223005 mRNA Translation: BAD96725.1 AE006464 Genomic DNA Translation: AAK61251.1 Sequence problems. Z98258 Genomic DNA No translation available. CH471112 Genomic DNA Translation: EAW85723.1 BC030248 mRNA Translation: AAH30248.1 BC129940 mRNA Translation: AAI29941.1 |
CCDSi | CCDS10425.1 [Q9H6Q4-1] CCDS76798.1 [Q9H6Q4-3] |
RefSeqi | NP_001291728.1, NM_001304799.1 [Q9H6Q4-3] NP_071938.1, NM_022493.2 [Q9H6Q4-1] |
Genome annotation databases
Ensembli | ENST00000251588; ENSP00000251588; ENSG00000103245 [Q9H6Q4-1] ENST00000540986; ENSP00000444008; ENSG00000103245 [Q9H6Q4-3] ENST00000568545; ENSP00000457058; ENSG00000103245 [Q9H6Q4-3] |
GeneIDi | 64428 |
KEGGi | hsa:64428 |
UCSCi | uc002cjp.4, human [Q9H6Q4-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY129231 mRNA Translation: AAM98737.1 AK025641 mRNA Translation: BAB15199.1 AK025861 mRNA Translation: BAB15261.1 AK095675 mRNA Translation: BAG53105.1 AK223005 mRNA Translation: BAD96725.1 AE006464 Genomic DNA Translation: AAK61251.1 Sequence problems. Z98258 Genomic DNA No translation available. CH471112 Genomic DNA Translation: EAW85723.1 BC030248 mRNA Translation: AAH30248.1 BC129940 mRNA Translation: AAI29941.1 |
CCDSi | CCDS10425.1 [Q9H6Q4-1] CCDS76798.1 [Q9H6Q4-3] |
RefSeqi | NP_001291728.1, NM_001304799.1 [Q9H6Q4-3] NP_071938.1, NM_022493.2 [Q9H6Q4-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 122176, 16 interactors |
CORUMi | Q9H6Q4 |
IntActi | Q9H6Q4, 7 interactors |
STRINGi | 9606.ENSP00000251588 |
PTM databases
iPTMneti | Q9H6Q4 |
MetOSitei | Q9H6Q4 |
PhosphoSitePlusi | Q9H6Q4 |
Genetic variation databases
BioMutai | NARFL |
DMDMi | 74733617 |
Proteomic databases
EPDi | Q9H6Q4 |
jPOSTi | Q9H6Q4 |
MassIVEi | Q9H6Q4 |
MaxQBi | Q9H6Q4 |
PaxDbi | Q9H6Q4 |
PeptideAtlasi | Q9H6Q4 |
PRIDEi | Q9H6Q4 |
ProteomicsDBi | 81009 [Q9H6Q4-1] 81010 [Q9H6Q4-2] 81011 [Q9H6Q4-3] |
Protocols and materials databases
Antibodypediai | 42404, 172 antibodies |
DNASUi | 64428 |
Genome annotation databases
Ensembli | ENST00000251588; ENSP00000251588; ENSG00000103245 [Q9H6Q4-1] ENST00000540986; ENSP00000444008; ENSG00000103245 [Q9H6Q4-3] ENST00000568545; ENSP00000457058; ENSG00000103245 [Q9H6Q4-3] |
GeneIDi | 64428 |
KEGGi | hsa:64428 |
UCSCi | uc002cjp.4, human [Q9H6Q4-1] |
Organism-specific databases
CTDi | 64428 |
DisGeNETi | 64428 |
GeneCardsi | CIAO3 |
HGNCi | HGNC:14179, CIAO3 |
HPAi | ENSG00000103245, Low tissue specificity |
MIMi | 611118, gene |
neXtProti | NX_Q9H6Q4 |
OpenTargetsi | ENSG00000103245 |
PharmGKBi | PA128394707 |
VEuPathDBi | HostDB:ENSG00000103245.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2439, Eukaryota |
GeneTreei | ENSGT00940000153514 |
HOGENOMi | CLU_018240_0_0_1 |
InParanoidi | Q9H6Q4 |
OMAi | FTWVVDT |
OrthoDBi | 705416at2759 |
PhylomeDBi | Q9H6Q4 |
TreeFami | TF106273 |
Enzyme and pathway databases
PathwayCommonsi | Q9H6Q4 |
Reactomei | R-HSA-2564830, Cytosolic iron-sulfur cluster assembly |
Miscellaneous databases
BioGRID-ORCSi | 64428, 754 hits in 875 CRISPR screens |
ChiTaRSi | CIAO3, human |
GenomeRNAii | 64428 |
Pharosi | Q9H6Q4, Tbio |
PROi | PR:Q9H6Q4 |
RNActi | Q9H6Q4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000103245, Expressed in right hemisphere of cerebellum and 179 other tissues |
ExpressionAtlasi | Q9H6Q4, baseline and differential |
Genevisiblei | Q9H6Q4, HS |
Family and domain databases
InterProi | View protein in InterPro IPR009016, Fe_hydrogenase IPR004108, Fe_hydrogenase_lsu_C IPR003149, Fe_hydrogenase_ssu |
Pfami | View protein in Pfam PF02906, Fe_hyd_lg_C, 1 hit PF02256, Fe_hyd_SSU, 1 hit |
SMARTi | View protein in SMART SM00902, Fe_hyd_SSU, 1 hit |
SUPFAMi | SSF53920, SSF53920, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CIAO3_HUMAN | |
Accessioni | Q9H6Q4Primary (citable) accession number: Q9H6Q4 Secondary accession number(s): A1L385 Q9H6J8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 29, 2007 |
Last sequence update: | March 1, 2001 | |
Last modified: | February 10, 2021 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families