UniProtKB - Q9H6E5 (STPAP_HUMAN)
Protein
Speckle targeted PIP5K1A-regulated poly(A) polymerase
Gene
TUT1
Organism
Homo sapiens (Human)
Status
Functioni
Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs (PubMed:18288197, PubMed:21102410). Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1 (PubMed:18288197). In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs (PubMed:21102410). In addition to adenylyltransferase activity, also has uridylyltransferase activity (PubMed:16790842, PubMed:18288197, PubMed:28589955). However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase (PubMed:18288197). Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA (PubMed:16790842, PubMed:18288197, PubMed:28589955). Not involved in replication-dependent histone mRNA degradation.4 Publications
Catalytic activityi
Cofactori
Activity regulationi
Adenylyltransferase activity is specifically phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2).1 Publication
Kineticsi
kcat is 0.059 sec(-1) with UTP as substrate. kcat is 0.002 sec(-1) with ATP as substrate. kcat is 0.061 sec(-1) with U6 snRNA-u4 as substrate.1 Publication
- KM=59 µM for UTP1 Publication
- KM=1380 µM for ATP1 Publication
- KM=55 µM for U6 snRNA-u41 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 216 | Magnesium or manganese; catalytic1 Publication | 1 | |
Binding sitei | 216 | UTP; via amide nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 218 | Magnesium or manganese; catalytic1 Publication | 1 | |
Binding sitei | 218 | UTPCombined sources1 Publication | 1 | |
Binding sitei | 392 | UTP or ATPCombined sources1 Publication | 1 | |
Binding sitei | 414 | UTPCombined sources1 Publication | 1 | |
Binding sitei | 432 | UTPCombined sources1 Publication | 1 | |
Binding sitei | 549 | UTP; via tele nitrogenCombined sources1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 16 – 46 | Matrin-typePROSITE-ProRule annotationAdd BLAST | 31 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- enzyme binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- mRNA 3'-UTR binding Source: UniProtKB
- nucleotidyltransferase activity Source: GO_Central
- polynucleotide adenylyltransferase activity Source: UniProtKB
- RNA binding Source: UniProtKB
- RNA uridylyltransferase activity Source: UniProtKB
- U6 snRNA binding Source: UniProtKB
GO - Biological processi
- mRNA polyadenylation Source: UniProtKB
- mRNA processing Source: GO_Central
- pre-mRNA cleavage required for polyadenylation Source: UniProtKB
- snRNA processing Source: UniProtKB
- U6 snRNA 3'-end processing Source: UniProtKB
Keywordsi
Molecular function | Nucleotidyltransferase, RNA-binding, Transferase |
Biological process | mRNA processing |
Ligand | ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9H6E5 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:TUT1 Synonyms:RBM21 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000149016.15 |
HGNCi | HGNC:26184, TUT1 |
MIMi | 610641, gene |
neXtProti | NX_Q9H6E5 |
Subcellular locationi
Nucleus
- nucleolus 1 Publication
- Nucleus speckle 2 Publications
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear speck Source: UniProtKB
- nucleolus Source: UniProtKB
- nucleoplasm Source: HPA
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 216 | D → A: Abolishes adenylyltransferase activity; when associated with A-218. 1 Publication | 1 | |
Mutagenesisi | 218 | D → A: Abolishes adenylyltransferase activity; when associated with A-216. 1 Publication | 1 | |
Mutagenesisi | 779 | R → A: Reduced terminal uridylyltransferase activity; when associated with A-783. 1 Publication | 1 | |
Mutagenesisi | 783 | R → A: Reduced terminal uridylyltransferase activity; when associated with A-779. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 64852 |
OpenTargetsi | ENSG00000149016 |
PharmGKBi | PA162407405 |
Miscellaneous databases
Pharosi | Q9H6E5, Tbio |
Polymorphism and mutation databases
BioMutai | TUT1 |
DMDMi | 126302611 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000254186 | 1 – 874 | Speckle targeted PIP5K1A-regulated poly(A) polymeraseAdd BLAST | 874 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 750 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylated by CK1 in the proline-rich (Pro-rich) region.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9H6E5 |
jPOSTi | Q9H6E5 |
MassIVEi | Q9H6E5 |
MaxQBi | Q9H6E5 |
PaxDbi | Q9H6E5 |
PeptideAtlasi | Q9H6E5 |
PRIDEi | Q9H6E5 |
ProteomicsDBi | 80983 |
PTM databases
iPTMneti | Q9H6E5 |
MetOSitei | Q9H6E5 |
PhosphoSitePlusi | Q9H6E5 |
Expressioni
Tissue specificityi
Widely expressed.1 Publication
Gene expression databases
Bgeei | ENSG00000149016, Expressed in dorsal plus ventral thalamus and 225 other tissues |
ExpressionAtlasi | Q9H6E5, baseline and differential |
Genevisiblei | Q9H6E5, HS |
Organism-specific databases
HPAi | ENSG00000149016, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsQ9H6E5
With | #Exp. | IntAct |
---|---|---|
KATNBL1 [Q9H079] | 3 | EBI-2511680,EBI-715394 |
PIP5K1A [Q99755] | 3 | EBI-2511680,EBI-726414 |
PIP5K1A - isoform 1 [Q99755-1] | 2 | EBI-2511680,EBI-15687389 |
GO - Molecular functioni
- enzyme binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 122325, 35 interactors |
DIPi | DIP-53651N |
IntActi | Q9H6E5, 31 interactors |
MINTi | Q9H6E5 |
STRINGi | 9606.ENSP00000308000 |
Miscellaneous databases
RNActi | Q9H6E5, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9H6E5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9H6E5 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 56 – 128 | RRMPROSITE-ProRule annotationAdd BLAST | 73 | |
Domaini | 491 – 549 | PAP-associatedSequence analysisAdd BLAST | 59 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 598 – 874 | KA1; binds the bulging loops of U6 snRNA but is dispensable for terminal uridylyltransferase activity1 PublicationAdd BLAST | 277 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 229 – 310 | Pro-richPROSITE-ProRule annotationAdd BLAST | 82 |
Domaini
The zinc-finger domain is required for terminal uridylyltransferase activity (PubMed:28589955). Together with the RRM domain, binds the 5'-area of U6 snRNA (PubMed:28589955).1 Publication
The RRM domain is required for terminal uridylyltransferase activity (PubMed:28589955). Together with the zinc-finger domain, binds the 5'-area of U6 snRNA (PubMed:28589955).1 Publication
The proline-rich region is dispensable for terminal uridylyltransferase activity.1 Publication
Sequence similaritiesi
Belongs to the DNA polymerase type-B-like family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 16 – 46 | Matrin-typePROSITE-ProRule annotationAdd BLAST | 31 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG2277, Eukaryota |
GeneTreei | ENSGT00940000159914 |
HOGENOMi | CLU_018757_1_0_1 |
InParanoidi | Q9H6E5 |
OrthoDBi | 1188122at2759 |
PhylomeDBi | Q9H6E5 |
Family and domain databases
CDDi | cd12279, RRM_TUT1, 1 hit |
Gene3Di | 3.30.460.10, 2 hits 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR043519, NT_sf IPR012677, Nucleotide-bd_a/b_plait_sf IPR002058, PAP_assoc IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR034389, Star-PAP IPR034388, Star-PAP_RRM IPR036236, Znf_C2H2_sf |
PANTHERi | PTHR12271:SF11, PTHR12271:SF11, 1 hit |
Pfami | View protein in Pfam PF03828, PAP_assoc, 1 hit PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit SSF57667, SSF57667, 1 hit SSF81301, SSF81301, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q9H6E5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAVDSDVES LPRGGFRCCL CHVTTANRPS LDAHLGGRKH RHLVELRAAR
60 70 80 90 100
KAQGLRSVFV SGFPRDVDSA QLSEYFLAFG PVASVVMDKD KGVFAIVEMG
110 120 130 140 150
DVGAREAVLS QSQHSLGGHR LRVRPREQKE FQSPASKSPK GAAPDSHQLA
160 170 180 190 200
KALAEAADVG AQMIKLVGLR ELSEAERQLR SLVVALMQEV FTEFFPGCVV
210 220 230 240 250
HPFGSSINSF DVHGCDLDLF LDLGDLEEPQ PVPKAPESPS LDSALASPLD
260 270 280 290 300
PQALACTPAS PPDSQPPASP QDSEALDFET PSSSLAPQTP DSALASETLA
310 320 330 340 350
SPQSLPPASP LLEDREEGDL GKASELAETP KEEKAEGAAM LELVGSILRG
360 370 380 390 400
CVPGVYRVQT VPSARRPVVK FCHRPSGLHG DVSLSNRLAL HNSRFLSLCS
410 420 430 440 450
ELDGRVRPLV YTLRCWAQGR GLSGSGPLLS NYALTLLVIY FLQTRDPPVL
460 470 480 490 500
PTVSQLTQKA GEGEQVEVDG WDCSFPRDAS RLEPSINVEP LSSLLAQFFS
510 520 530 540 550
CVSCWDLRGS LLSLREGQAL PVAGGLPSNL WEGLRLGPLN LQDPFDLSHN
560 570 580 590 600
VAANVTSRVA GRLQNCCRAA ANYCRSLQYQ RRSSRGRDWG LLPLLQPSSP
610 620 630 640 650
SSLLSATPIP LPLAPFTQLT AALVQVFREA LGCHIEQATK RTRSEGGGTG
660 670 680 690 700
ESSQGGTSKR LKVDGQKNCC EEGKEEQQGC AGDGGEDRVE EMVIEVGEMV
710 720 730 740 750
QDWAMQSPGQ PGDLPLTTGK HGAPGEEGQP SHAALAERGP KGHEAAQEWS
760 770 780 790 800
QGEAGKGASL PSSASWRCAL WHRVWQGRRR ARRRLQQQTK EGAGGGAGTR
810 820 830 840 850
AGWLATEAQV TQELKGLSGG EERPETEPLL SFVASVSPAD RMLTVTPLQD
860 870
PQGLFPDLHH FLQVFLPQAI RHLK
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF5H0R1 | F5H0R1_HUMAN | RNA-binding motif protein 21 | TUT1 | 912 | Annotation score: | ||
C9JBX0 | C9JBX0_HUMAN | Speckle targeted PIP5K1A-regulated ... | TUT1 | 143 | Annotation score: | ||
J3KN81 | J3KN81_HUMAN | Speckle targeted PIP5K1A-regulated ... | TUT1 | 165 | Annotation score: | ||
F8WA97 | F8WA97_HUMAN | Speckle targeted PIP5K1A-regulated ... | TUT1 | 100 | Annotation score: |
Sequence cautioni
The sequence BAB15282 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 66 | D → G in BAB15314 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 809 | Q → R in BAF85299 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_028833 | 442 | L → F. Corresponds to variant dbSNP:rs3197865Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK025920 mRNA Translation: BAB15282.1 Different initiation. AK026000 mRNA Translation: BAB15314.1 AK292610 mRNA Translation: BAF85299.1 AP002990 Genomic DNA No translation available. CH471076 Genomic DNA Translation: EAW74029.1 BC005013 mRNA Translation: AAH05013.2 BC110910 mRNA Translation: AAI10911.1 BC128263 mRNA Translation: AAI28264.1 |
CCDSi | CCDS8021.2 |
RefSeqi | NP_073741.2, NM_022830.2 |
Genome annotation databases
Ensembli | ENST00000476907; ENSP00000419607; ENSG00000149016 |
GeneIDi | 64852 |
KEGGi | hsa:64852 |
UCSCi | uc058cig.1, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK025920 mRNA Translation: BAB15282.1 Different initiation. AK026000 mRNA Translation: BAB15314.1 AK292610 mRNA Translation: BAF85299.1 AP002990 Genomic DNA No translation available. CH471076 Genomic DNA Translation: EAW74029.1 BC005013 mRNA Translation: AAH05013.2 BC110910 mRNA Translation: AAI10911.1 BC128263 mRNA Translation: AAI28264.1 |
CCDSi | CCDS8021.2 |
RefSeqi | NP_073741.2, NM_022830.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2E5G | NMR | - | A | 55-141 | [»] | |
5WU1 | X-ray | 2.80 | A/B | 141-874 | [»] | |
5WU2 | X-ray | 2.95 | A/B | 141-874 | [»] | |
5WU3 | X-ray | 2.70 | A/B | 141-874 | [»] | |
5WU4 | X-ray | 2.80 | A/B | 141-874 | [»] | |
5WU5 | X-ray | 3.40 | A/B/C/D | 141-874 | [»] | |
5WU6 | X-ray | 3.21 | A/B/C/D | 53-599 | [»] | |
SMRi | Q9H6E5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 122325, 35 interactors |
DIPi | DIP-53651N |
IntActi | Q9H6E5, 31 interactors |
MINTi | Q9H6E5 |
STRINGi | 9606.ENSP00000308000 |
PTM databases
iPTMneti | Q9H6E5 |
MetOSitei | Q9H6E5 |
PhosphoSitePlusi | Q9H6E5 |
Polymorphism and mutation databases
BioMutai | TUT1 |
DMDMi | 126302611 |
Proteomic databases
EPDi | Q9H6E5 |
jPOSTi | Q9H6E5 |
MassIVEi | Q9H6E5 |
MaxQBi | Q9H6E5 |
PaxDbi | Q9H6E5 |
PeptideAtlasi | Q9H6E5 |
PRIDEi | Q9H6E5 |
ProteomicsDBi | 80983 |
Protocols and materials databases
Antibodypediai | 28452, 79 antibodies |
Genome annotation databases
Ensembli | ENST00000476907; ENSP00000419607; ENSG00000149016 |
GeneIDi | 64852 |
KEGGi | hsa:64852 |
UCSCi | uc058cig.1, human |
Organism-specific databases
CTDi | 64852 |
DisGeNETi | 64852 |
EuPathDBi | HostDB:ENSG00000149016.15 |
GeneCardsi | TUT1 |
HGNCi | HGNC:26184, TUT1 |
HPAi | ENSG00000149016, Low tissue specificity |
MIMi | 610641, gene |
neXtProti | NX_Q9H6E5 |
OpenTargetsi | ENSG00000149016 |
PharmGKBi | PA162407405 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2277, Eukaryota |
GeneTreei | ENSGT00940000159914 |
HOGENOMi | CLU_018757_1_0_1 |
InParanoidi | Q9H6E5 |
OrthoDBi | 1188122at2759 |
PhylomeDBi | Q9H6E5 |
Enzyme and pathway databases
PathwayCommonsi | Q9H6E5 |
Miscellaneous databases
BioGRID-ORCSi | 64852, 744 hits in 838 CRISPR screens |
ChiTaRSi | TUT1, human |
EvolutionaryTracei | Q9H6E5 |
GenomeRNAii | 64852 |
Pharosi | Q9H6E5, Tbio |
PROi | PR:Q9H6E5 |
RNActi | Q9H6E5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000149016, Expressed in dorsal plus ventral thalamus and 225 other tissues |
ExpressionAtlasi | Q9H6E5, baseline and differential |
Genevisiblei | Q9H6E5, HS |
Family and domain databases
CDDi | cd12279, RRM_TUT1, 1 hit |
Gene3Di | 3.30.460.10, 2 hits 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR043519, NT_sf IPR012677, Nucleotide-bd_a/b_plait_sf IPR002058, PAP_assoc IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR034389, Star-PAP IPR034388, Star-PAP_RRM IPR036236, Znf_C2H2_sf |
PANTHERi | PTHR12271:SF11, PTHR12271:SF11, 1 hit |
Pfami | View protein in Pfam PF03828, PAP_assoc, 1 hit PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit SSF57667, SSF57667, 1 hit SSF81301, SSF81301, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | STPAP_HUMAN | |
Accessioni | Q9H6E5Primary (citable) accession number: Q9H6E5 Secondary accession number(s): A1A527 Q9H6H7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2006 |
Last sequence update: | February 20, 2007 | |
Last modified: | December 2, 2020 | |
This is version 150 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations