UniProtKB - Q9H5V8 (CDCP1_HUMAN)
Protein
CUB domain-containing protein 1
Gene
CDCP1
Organism
Homo sapiens (Human)
Status
Functioni
May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.6 Publications
Enzyme and pathway databases
PathwayCommonsi | Q9H5V8 |
SIGNORi | Q9H5V8 |
Names & Taxonomyi
Protein namesi | Recommended name: CUB domain-containing protein 1Alternative name(s): Membrane glycoprotein gp140 Subtractive immunization M plus HEp3-associated 135 kDa protein Short name: SIMA135 Transmembrane and associated with src kinases CD_antigen: CD318 |
Gene namesi | Name:CDCP1 Synonyms:TRASK ORF Names:UNQ2486/PRO5773 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:24357, CDCP1 |
MIMi | 611735, gene |
neXtProti | NX_Q9H5V8 |
VEuPathDBi | HostDB:ENSG00000163814.7 |
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Single-pass membrane protein Curated
Note: Shedding may also lead to a soluble peptide.
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 30 – 667 | ExtracellularSequence analysisAdd BLAST | 638 | |
Transmembranei | 668 – 688 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 689 – 836 | CytoplasmicSequence analysisAdd BLAST | 148 |
Keywords - Cellular componenti
Cell membrane, Membrane, SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 734 | Y → F: Impaired association with SRC. 1 Publication | 1 | |
Mutagenesisi | 762 | Y → F: Impaired association with protein kinase PRKCG but not with SRC. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 64866 |
OpenTargetsi | ENSG00000163814 |
PharmGKBi | PA142672140 |
Miscellaneous databases
Pharosi | Q9H5V8, Tbio |
Genetic variation databases
BioMutai | CDCP1 |
DMDMi | 317373455 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 29 | 2 PublicationsAdd BLAST | 29 | |
ChainiPRO_0000226249 | 30 – 836 | CUB domain-containing protein 1Add BLAST | 807 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 39 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 122 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 180 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 205 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 270 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 310 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 386 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 476 ↔ 499 | By similarity | ||
Modified residuei | 734 | Phosphotyrosine1 Publication | 1 |
Post-translational modificationi
Phosphorylated on tyrosine by kinases of the SRC family such as SRC and YES as well as by the protein kinase C gamma/PRKCG. Dephosphorylated by phosphotyrosine phosphatases. Also phosphorylated by suramin, a heparin analog. Tyrosine phosphorylated in response to dissociation of integrin alpha-6 beta-4 from laminin-5.4 Publications
N-glycosylated.3 Publications
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). Another peptide of 80 kDa (p80) is present in cultured keratinocytes probably due to tryptic cleavage at an unidentified site on its N-terminal side. Converted to p80 by plasmin, a trypsin-like protease.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 368 – 369 | Cleavage; by ST14/MT-SP1 | 2 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
CPTACi | CPTAC-2211 |
EPDi | Q9H5V8 |
jPOSTi | Q9H5V8 |
MassIVEi | Q9H5V8 |
MaxQBi | Q9H5V8 |
PaxDbi | Q9H5V8 |
PeptideAtlasi | Q9H5V8 |
PRIDEi | Q9H5V8 |
ProteomicsDBi | 80933 [Q9H5V8-1] 80934 [Q9H5V8-2] 80935 [Q9H5V8-3] |
PTM databases
GlyConnecti | 1160, 13 N-Linked glycans (8 sites) |
GlyGeni | Q9H5V8, 10 sites |
iPTMneti | Q9H5V8 |
PhosphoSitePlusi | Q9H5V8 |
SwissPalmi | Q9H5V8 |
Expressioni
Tissue specificityi
Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.4 Publications
Gene expression databases
Bgeei | ENSG00000163814, Expressed in squamous epithelium and 158 other tissues |
Genevisiblei | Q9H5V8, HS |
Organism-specific databases
HPAi | ENSG00000163814, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with CDH2/N-cadherin, CDH3/P-cadherin, SDC1/syndecan-1, SDC4/syndecan-4 and the serine protease ST14/MT-SP1.
Also interacts with SRC and PRKCG/protein kinase C gamma.
2 PublicationsBinary interactionsi
Hide detailsQ9H5V8
With | #Exp. | IntAct |
---|---|---|
SDCBP [O00560] | 6 | EBI-1019736,EBI-727004 |
SRC [P12931] | 3 | EBI-1019736,EBI-621482 |
YES1 [P07947] | 2 | EBI-1019736,EBI-515331 |
Protein-protein interaction databases
BioGRIDi | 122336, 15 interactors |
CORUMi | Q9H5V8 |
DIPi | DIP-33861N |
IntActi | Q9H5V8, 26 interactors |
MINTi | Q9H5V8 |
STRINGi | 9606.ENSP00000296129 |
Miscellaneous databases
RNActi | Q9H5V8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 417 – 544 | CUBAdd BLAST | 128 |
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QVKN, Eukaryota |
GeneTreei | ENSGT00390000010209 |
HOGENOMi | CLU_007498_0_0_1 |
InParanoidi | Q9H5V8 |
OMAi | ISNCSPM |
OrthoDBi | 223122at2759 |
PhylomeDBi | Q9H5V8 |
TreeFami | TF331392 |
Family and domain databases
InterProi | View protein in InterPro IPR038811, CDCP1 IPR035914, Sperma_CUB_dom_sf |
PANTHERi | PTHR14477, PTHR14477, 1 hit |
SUPFAMi | SSF49854, SSF49854, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q9H5V8-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGLNCGVSI ALLGVLLLGA ARLPRGAEAF EIALPRESNI TVLIKLGTPT
60 70 80 90 100
LLAKPCYIVI SKRHITMLSI KSGERIVFTF SCQSPENHFV IEIQKNIDCM
110 120 130 140 150
SGPCPFGEVQ LQPSTSLLPT LNRTFIWDVK AHKSIGLELQ FSIPRLRQIG
160 170 180 190 200
PGESCPDGVT HSISGRIDAT VVRIGTFCSN GTVSRIKMQE GVKMALHLPW
210 220 230 240 250
FHPRNVSGFS IANRSSIKRL CIIESVFEGE GSATLMSANY PEGFPEDELM
260 270 280 290 300
TWQFVVPAHL RASVSFLNFN LSNCERKEER VEYYIPGSTT NPEVFKLEDK
310 320 330 340 350
QPGNMAGNFN LSLQGCDQDA QSPGILRLQF QVLVQHPQNE SNKIYVVDLS
360 370 380 390 400
NERAMSLTIE PRPVKQSRKF VPGCFVCLES RTCSSNLTLT SGSKHKISFL
410 420 430 440 450
CDDLTRLWMN VEKTISCTDH RYCQRKSYSL QVPSDILHLP VELHDFSWKL
460 470 480 490 500
LVPKDRLSLV LVPAQKLQQH THEKPCNTSF SYLVASAIPS QDLYFGSFCP
510 520 530 540 550
GGSIKQIQVK QNISVTLRTF APSFQQEASR QGLTVSFIPY FKEEGVFTVT
560 570 580 590 600
PDTKSKVYLR TPNWDRGLPS LTSVSWNISV PRDQVACLTF FKERSGVVCQ
610 620 630 640 650
TGRAFMIIQE QRTRAEEIFS LDEDVLPKPS FHHHSFWVNI SNCSPTSGKQ
660 670 680 690 700
LDLLFSVTLT PRTVDLTVIL IAAVGGGVLL LSALGLIICC VKKKKKKTNK
710 720 730 740 750
GPAVGIYNDN INTEMPRQPK KFQKGRKDND SHVYAVIEDT MVYGHLLQDS
760 770 780 790 800
SGSFLQPEVD TYRPFQGTMG VCPPSPPTIC SRAPTAKLAT EEPPPRSPPE
810 820 830
SESEPYTFSH PNNGDVSSKD TDIPLLNTQE PMEPAE
Sequence cautioni
The sequence BAB15388 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 252 | W → C in BAB14695 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 284 | Y → D in BAB15388 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 434 | S → G in BAB14695 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 466 | K → R in BAB15388 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 827 | N → S in AAO33397 (PubMed:12660814).Curated | 1 | |
Sequence conflicti | 827 | N → S in BAB15511 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_025498 | 525 | Q → R3 PublicationsCorresponds to variant dbSNP:rs3749191Ensembl. | 1 | |
Natural variantiVAR_055095 | 673 | A → V1 PublicationCorresponds to variant dbSNP:rs35428731Ensembl. | 1 | |
Natural variantiVAR_025499 | 709 | D → G3 PublicationsCorresponds to variant dbSNP:rs9874077Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_017432 | 1 – 187 | Missing in isoform 2. 1 PublicationAdd BLAST | 187 | |
Alternative sequenceiVSP_017433 | 342 – 343 | NK → SE in isoform 3. 2 Publications | 2 | |
Alternative sequenceiVSP_017434 | 344 – 836 | Missing in isoform 3. 2 PublicationsAdd BLAST | 493 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY026461 mRNA Translation: AAK02058.1 AF468010 mRNA Translation: AAO33397.1 AY167484 mRNA Translation: AAO34538.1 AY358779 mRNA Translation: AAQ89139.1 AK023834 mRNA Translation: BAB14695.1 AK026187 mRNA Translation: BAB15388.1 Different initiation. AK026622 mRNA Translation: BAB15511.1 AC104165 Genomic DNA No translation available. AC105902 Genomic DNA No translation available. BC021099 mRNA Translation: AAH21099.1 BC069254 mRNA Translation: AAH69254.1 AY375452 mRNA Translation: AAR21289.1 |
CCDSi | CCDS2727.1 [Q9H5V8-1] CCDS46812.1 [Q9H5V8-3] |
RefSeqi | NP_073753.3, NM_022842.4 [Q9H5V8-1] NP_835488.1, NM_178181.2 [Q9H5V8-3] |
Genome annotation databases
Ensembli | ENST00000296129; ENSP00000296129; ENSG00000163814 [Q9H5V8-1] ENST00000425231; ENSP00000399342; ENSG00000163814 [Q9H5V8-3] |
GeneIDi | 64866 |
KEGGi | hsa:64866 |
UCSCi | uc003com.5, human [Q9H5V8-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY026461 mRNA Translation: AAK02058.1 AF468010 mRNA Translation: AAO33397.1 AY167484 mRNA Translation: AAO34538.1 AY358779 mRNA Translation: AAQ89139.1 AK023834 mRNA Translation: BAB14695.1 AK026187 mRNA Translation: BAB15388.1 Different initiation. AK026622 mRNA Translation: BAB15511.1 AC104165 Genomic DNA No translation available. AC105902 Genomic DNA No translation available. BC021099 mRNA Translation: AAH21099.1 BC069254 mRNA Translation: AAH69254.1 AY375452 mRNA Translation: AAR21289.1 |
CCDSi | CCDS2727.1 [Q9H5V8-1] CCDS46812.1 [Q9H5V8-3] |
RefSeqi | NP_073753.3, NM_022842.4 [Q9H5V8-1] NP_835488.1, NM_178181.2 [Q9H5V8-3] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 122336, 15 interactors |
CORUMi | Q9H5V8 |
DIPi | DIP-33861N |
IntActi | Q9H5V8, 26 interactors |
MINTi | Q9H5V8 |
STRINGi | 9606.ENSP00000296129 |
PTM databases
GlyConnecti | 1160, 13 N-Linked glycans (8 sites) |
GlyGeni | Q9H5V8, 10 sites |
iPTMneti | Q9H5V8 |
PhosphoSitePlusi | Q9H5V8 |
SwissPalmi | Q9H5V8 |
Genetic variation databases
BioMutai | CDCP1 |
DMDMi | 317373455 |
Proteomic databases
CPTACi | CPTAC-2211 |
EPDi | Q9H5V8 |
jPOSTi | Q9H5V8 |
MassIVEi | Q9H5V8 |
MaxQBi | Q9H5V8 |
PaxDbi | Q9H5V8 |
PeptideAtlasi | Q9H5V8 |
PRIDEi | Q9H5V8 |
ProteomicsDBi | 80933 [Q9H5V8-1] 80934 [Q9H5V8-2] 80935 [Q9H5V8-3] |
Protocols and materials databases
ABCDi | Q9H5V8, 13 sequenced antibodies |
Antibodypediai | 2564, 802 antibodies |
DNASUi | 64866 |
Genome annotation databases
Ensembli | ENST00000296129; ENSP00000296129; ENSG00000163814 [Q9H5V8-1] ENST00000425231; ENSP00000399342; ENSG00000163814 [Q9H5V8-3] |
GeneIDi | 64866 |
KEGGi | hsa:64866 |
UCSCi | uc003com.5, human [Q9H5V8-1] |
Organism-specific databases
CTDi | 64866 |
DisGeNETi | 64866 |
GeneCardsi | CDCP1 |
HGNCi | HGNC:24357, CDCP1 |
HPAi | ENSG00000163814, Low tissue specificity |
MIMi | 611735, gene |
neXtProti | NX_Q9H5V8 |
OpenTargetsi | ENSG00000163814 |
PharmGKBi | PA142672140 |
VEuPathDBi | HostDB:ENSG00000163814.7 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QVKN, Eukaryota |
GeneTreei | ENSGT00390000010209 |
HOGENOMi | CLU_007498_0_0_1 |
InParanoidi | Q9H5V8 |
OMAi | ISNCSPM |
OrthoDBi | 223122at2759 |
PhylomeDBi | Q9H5V8 |
TreeFami | TF331392 |
Enzyme and pathway databases
PathwayCommonsi | Q9H5V8 |
SIGNORi | Q9H5V8 |
Miscellaneous databases
BioGRID-ORCSi | 64866, 1 hit in 876 CRISPR screens |
ChiTaRSi | CDCP1, human |
GeneWikii | CDCP1 |
GenomeRNAii | 64866 |
Pharosi | Q9H5V8, Tbio |
PROi | PR:Q9H5V8 |
RNActi | Q9H5V8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000163814, Expressed in squamous epithelium and 158 other tissues |
Genevisiblei | Q9H5V8, HS |
Family and domain databases
InterProi | View protein in InterPro IPR038811, CDCP1 IPR035914, Sperma_CUB_dom_sf |
PANTHERi | PTHR14477, PTHR14477, 1 hit |
SUPFAMi | SSF49854, SSF49854, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CDCP1_HUMAN | |
Accessioni | Q9H5V8Primary (citable) accession number: Q9H5V8 Secondary accession number(s): Q49UB4 Q9H8C2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 7, 2006 |
Last sequence update: | January 11, 2011 | |
Last modified: | February 10, 2021 | |
This is version 145 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human cell differentiation molecules
CD nomenclature of surface proteins of human leucocytes and list of entries - Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot