UniProtKB - Q9H4L5 (OSBL3_HUMAN)
Oxysterol-binding protein-related protein 3
OSBPL3
Functioni
GO - Molecular functioni
- cholesterol binding Source: BHF-UCL
- sterol binding Source: GO_Central
- sterol transporter activity Source: GO_Central
GO - Biological processi
- bile acid biosynthetic process Source: Reactome
Keywordsi
Biological process | Lipid transport, Transport |
Ligand | Lipid-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9H4L5 |
Reactomei | R-HSA-192105, Synthesis of bile acids and bile salts |
Names & Taxonomyi
Protein namesi | Recommended name: Oxysterol-binding protein-related protein 3Short name: ORP-3 Short name: OSBP-related protein 3 |
Gene namesi | Name:OSBPL3 Synonyms:KIAA0704, ORP3, OSBP3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16370, OSBPL3 |
MIMi | 606732, gene |
neXtProti | NX_Q9H4L5 |
VEuPathDBi | HostDB:ENSG00000070882.12 |
Subcellular locationi
Plasma membrane
- Cell membrane 4 Publications; Peripheral membrane protein 4 Publications
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Peripheral membrane protein 1 Publication
Cytoplasm and Cytosol
- cytosol 1 Publication
Nucleus
- Nucleus membrane 1 Publication; Peripheral membrane protein Curated
Other locations
- filopodium tip 1 Publication
Note: Co-localizes with OSBPL6 at contact sites between the plasma membrane and the endoplasmic reticulum.By similarity
Cytosol
- cytosol Source: BHF-UCL
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
- perinuclear endoplasmic reticulum Source: BHF-UCL
Nucleus
- nuclear membrane Source: BHF-UCL
Plasma Membrane
- plasma membrane Source: BHF-UCL
Other locations
- filopodium tip Source: UniProtKB-SubCell
- intracellular membrane-bounded organelle Source: GO_Central
- membrane Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Endoplasmic reticulum, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 60 | K → I: Abolishes plasma membrane targeting and nuclear localization. 1 Publication | 1 | |
Mutagenesisi | 61 | K → A: Abolishes plasma membrane targeting and nuclear localization. 1 Publication | 1 | |
Mutagenesisi | 71 | K → A: Abolishes plasma membrane targeting and nuclear localization. 1 Publication | 1 | |
Mutagenesisi | 72 | R → I: Abolishes plasma membrane targeting and nuclear localization. 1 Publication | 1 | |
Mutagenesisi | 97 | G → E: Decreases plasma membrane targeting and nuclear localization. 1 Publication | 1 | |
Mutagenesisi | 162 – 167 | FFSGST → AAAAAA: No effect on interaction with VAPA. 1 Publication | 6 | |
Mutagenesisi | 451 – 453 | FFD → VVV: Reduces VAPA binding. Abolishes association with endoplasmic reticulum. 2 Publications | 3 |
Organism-specific databases
DisGeNETi | 26031 |
OpenTargetsi | ENSG00000070882 |
PharmGKBi | PA32828 |
Miscellaneous databases
Pharosi | Q9H4L5, Tbio |
Genetic variation databases
BioMutai | OSBPL3 |
DMDMi | 20139176 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000100371 | 1 – 887 | Oxysterol-binding protein-related protein 3Add BLAST | 887 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 16 | PhosphoserineCombined sources | 1 | |
Modified residuei | 34 | PhosphoserineCombined sources | 1 | |
Modified residuei | 200 | PhosphoserineCombined sources | 1 | |
Modified residuei | 251 | PhosphoserineCombined sources | 1 | |
Modified residuei | 265 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 304 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 309 | PhosphoserineCombined sources | 1 | |
Modified residuei | 320 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 323 | Phosphoserine1 Publication | 1 | |
Modified residuei | 371 | Phosphoserine1 Publication | 1 | |
Modified residuei | 372 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 410 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 425 | PhosphoserineCombined sources | 1 | |
Modified residuei | 437 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 440 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9H4L5 |
jPOSTi | Q9H4L5 |
MassIVEi | Q9H4L5 |
MaxQBi | Q9H4L5 |
PaxDbi | Q9H4L5 |
PeptideAtlasi | Q9H4L5 |
PRIDEi | Q9H4L5 |
ProteomicsDBi | 80852 [Q9H4L5-1] 80853 [Q9H4L5-2] 80854 [Q9H4L5-3] 80855 [Q9H4L5-4] 80856 [Q9H4L5-5] 80857 [Q9H4L5-6] 80858 [Q9H4L5-7] 80859 [Q9H4L5-8] |
PTM databases
iPTMneti | Q9H4L5 |
PhosphoSitePlusi | Q9H4L5 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSG00000070882, Expressed in cortex of kidney and 199 other tissues |
ExpressionAtlasi | Q9H4L5, baseline and differential |
Genevisiblei | Q9H4L5, HS |
Organism-specific databases
HPAi | ENSG00000070882, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsQ9H4L5
Protein-protein interaction databases
BioGRIDi | 117497, 79 interactors |
ELMi | Q9H4L5 |
IntActi | Q9H4L5, 56 interactors |
MINTi | Q9H4L5 |
STRINGi | 9606.ENSP00000315410 |
Miscellaneous databases
RNActi | Q9H4L5, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 51 – 146 | PHPROSITE-ProRule annotationAdd BLAST | 96 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 161 – 167 | FFAT 11 Publication | 7 | |
Motifi | 450 – 454 | FFAT 21 Publication | 5 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1737, Eukaryota |
GeneTreei | ENSGT00940000155957 |
HOGENOMi | CLU_007105_4_1_1 |
InParanoidi | Q9H4L5 |
OMAi | XAKYWST |
OrthoDBi | 542090at2759 |
PhylomeDBi | Q9H4L5 |
TreeFami | TF320922 |
Family and domain databases
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR037239, OSBP_sf IPR000648, Oxysterol-bd IPR018494, Oxysterol-bd_CS IPR011993, PH-like_dom_sf IPR041680, PH_8 IPR001849, PH_domain |
PANTHERi | PTHR10972, PTHR10972, 1 hit |
Pfami | View protein in Pfam PF01237, Oxysterol_BP, 1 hit PF15409, PH_8, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit |
SUPFAMi | SSF144000, SSF144000, 1 hit |
PROSITEi | View protein in PROSITE PS01013, OSBP, 1 hit PS50003, PH_DOMAIN, 1 hit |
s (8+)i Sequence
Sequence statusi: Complete.
This entry describes 8 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 8 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MMSDEKNLGV SQKLVSPSRS TSSCSSKQGS RQDSWEVVEG LRGEMNYTQE
60 70 80 90 100
PPVQKGFLLK KRKWPLKGWH KRFFYLDKGI LKYAKSQTDI EREKLHGCID
110 120 130 140 150
VGLSVMSVKK SSKCIDLDTE EHIYHLKVKS EEVFDEWVSK LRHHRMYRQN
160 170 180 190 200
EIAMFPHEVN HFFSGSTITD SSSGVFDSIS SRKRSSISKQ NLFQTGSNVS
210 220 230 240 250
FSCGGETRVP LWLQSSEDME KCSKDLAHCH AYLVEMSQLL QSMDVLHRTY
260 270 280 290 300
SAPAINAIQG GSFESPKKEK RSHRRWRSRA IGKDAKGTLQ VPKPFSGPVR
310 320 330 340 350
LHSSNPNLST LDFGEEKNYS DGSETSSEFS KMQEDLCHIA HKVYFTLRSA
360 370 380 390 400
FNIMSAEREK LKQLMEQDAS SSPSAQVIGL KNALSSALAQ NTDLKERLRR
410 420 430 440 450
IHAESLLLDS PAVAKSGDNL AEENSRDENR ALVHQLSNES RLSITDSLSE
460 470 480 490 500
FFDAQEVLLS PSSSENEISD DDSYVSDISD NLSLDNLSND LDNERQTLGP
510 520 530 540 550
VLDSGREAKS RRRTCLPAPC PSSSNISLWN ILRNNIGKDL SKVAMPVELN
560 570 580 590 600
EPLNTLQRLC EELEYSELLD KAAQIPSPLE RMVYVAAFAI SAYASSYYRA
610 620 630 640 650
GSKPFNPVLG ETYECIREDK GFQFFSEQVS HHPPISACHA ESRNFVFWQD
660 670 680 690 700
VRWKNKFWGK SMEIVPIGTT HVTLPVFGDH FEWNKVTSCI HNILSGQRWI
710 720 730 740 750
EHYGEIVIKN LHDDSCYCKV NFIKAKYWST NAHEIEGTVF DRSGKAVHRL
760 770 780 790 800
FGKWHESIYC GGGSSSACVW RANPMPKGYE QYYSFTQFAL ELNEMDPSSK
810 820 830 840 850
SLLPPTDTRF RPDQRFLEEG NLEEAEIQKQ RIEQLQRERR RVLEENHVEH
860 870 880
QPRFFRKSDD DSWVSNGTYL ELRKDLGFSK LDHPVLW
The sequence of this isoform differs from the canonical sequence as follows:
259-289: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
387-422: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
259-289: Missing.
387-422: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
584-631: YVAAFAISAY...FQFFSEQVSH → RSQPSLATVQ...SSAWLFPVTL
632-887: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
259-289: Missing.
584-631: YVAAFAISAY...FQFFSEQVSH → RSQPSLATVQ...SSAWLFPVTL
632-887: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
387-422: Missing.
584-631: YVAAFAISAY...FQFFSEQVSH → RSQPSLATVQ...SSAWLFPVTL
632-887: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
259-289: Missing.
387-422: Missing.
584-631: YVAAFAISAY...FQFFSEQVSH → RSQPSLATVQ...SSAWLFPVTL
632-887: Missing.
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JEF2 | C9JEF2_HUMAN | Oxysterol-binding protein-related p... | OSBPL3 | 89 | Annotation score: | ||
C9JZ19 | C9JZ19_HUMAN | Oxysterol-binding protein-related p... | OSBPL3 | 112 | Annotation score: | ||
C9J8P4 | C9J8P4_HUMAN | Oxysterol-binding protein-related p... | OSBPL3 | 61 | Annotation score: |
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_053548 | 354 | M → V. Corresponds to variant dbSNP:rs11768296Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008219 | 259 – 289 | Missing in isoform 1b, isoform 1d, isoform 2b and isoform 2d. 1 PublicationAdd BLAST | 31 | |
Alternative sequenceiVSP_008220 | 387 – 422 | Missing in isoform 1c, isoform 1d, isoform 2c and isoform 2d. 1 PublicationAdd BLAST | 36 | |
Alternative sequenceiVSP_008221 | 584 – 631 | YVAAF…EQVSH → RSQPSLATVQPRSPSHEAIH GAHQRDSPCSLRFHFDCSVN RFITQSCLASSAWLFPVTL in isoform 2a, isoform 2b, isoform 2c and isoform 2d. 1 PublicationAdd BLAST | 48 | |
Alternative sequenceiVSP_008222 | 632 – 887 | Missing in isoform 2a, isoform 2b, isoform 2c and isoform 2d. 1 PublicationAdd BLAST | 256 |
Sequence databases
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
7CYZ | X-ray | 2.10 | A/B | 511-887 | [»] | |
SMRi | Q9H4L5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 117497, 79 interactors |
ELMi | Q9H4L5 |
IntActi | Q9H4L5, 56 interactors |
MINTi | Q9H4L5 |
STRINGi | 9606.ENSP00000315410 |
PTM databases
iPTMneti | Q9H4L5 |
PhosphoSitePlusi | Q9H4L5 |
Genetic variation databases
BioMutai | OSBPL3 |
DMDMi | 20139176 |
Proteomic databases
EPDi | Q9H4L5 |
jPOSTi | Q9H4L5 |
MassIVEi | Q9H4L5 |
MaxQBi | Q9H4L5 |
PaxDbi | Q9H4L5 |
PeptideAtlasi | Q9H4L5 |
PRIDEi | Q9H4L5 |
ProteomicsDBi | 80852 [Q9H4L5-1] 80853 [Q9H4L5-2] 80854 [Q9H4L5-3] 80855 [Q9H4L5-4] 80856 [Q9H4L5-5] 80857 [Q9H4L5-6] 80858 [Q9H4L5-7] 80859 [Q9H4L5-8] |
Protocols and materials databases
Antibodypediai | 12224, 110 antibodies |
Genome annotation databases
Organism-specific databases
CTDi | 26031 |
DisGeNETi | 26031 |
GeneCardsi | OSBPL3 |
HGNCi | HGNC:16370, OSBPL3 |
HPAi | ENSG00000070882, Low tissue specificity |
MIMi | 606732, gene |
neXtProti | NX_Q9H4L5 |
OpenTargetsi | ENSG00000070882 |
PharmGKBi | PA32828 |
VEuPathDBi | HostDB:ENSG00000070882.12 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1737, Eukaryota |
GeneTreei | ENSGT00940000155957 |
HOGENOMi | CLU_007105_4_1_1 |
InParanoidi | Q9H4L5 |
OMAi | XAKYWST |
OrthoDBi | 542090at2759 |
PhylomeDBi | Q9H4L5 |
TreeFami | TF320922 |
Enzyme and pathway databases
PathwayCommonsi | Q9H4L5 |
Reactomei | R-HSA-192105, Synthesis of bile acids and bile salts |
Miscellaneous databases
BioGRID-ORCSi | 26031, 8 hits in 999 CRISPR screens |
ChiTaRSi | OSBPL3, human |
GeneWikii | OSBPL3 |
GenomeRNAii | 26031 |
Pharosi | Q9H4L5, Tbio |
PROi | PR:Q9H4L5 |
RNActi | Q9H4L5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000070882, Expressed in cortex of kidney and 199 other tissues |
ExpressionAtlasi | Q9H4L5, baseline and differential |
Genevisiblei | Q9H4L5, HS |
Family and domain databases
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR037239, OSBP_sf IPR000648, Oxysterol-bd IPR018494, Oxysterol-bd_CS IPR011993, PH-like_dom_sf IPR041680, PH_8 IPR001849, PH_domain |
PANTHERi | PTHR10972, PTHR10972, 1 hit |
Pfami | View protein in Pfam PF01237, Oxysterol_BP, 1 hit PF15409, PH_8, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit |
SUPFAMi | SSF144000, SSF144000, 1 hit |
PROSITEi | View protein in PROSITE PS01013, OSBP, 1 hit PS50003, PH_DOMAIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OSBL3_HUMAN | |
Accessioni | Q9H4L5Primary (citable) accession number: Q9H4L5 Secondary accession number(s): A4D167 Q9UED6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 11, 2002 |
Last sequence update: | March 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families