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Protein

Sentrin-specific protease 3

Gene

SENP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. Deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability. Deconjugates SUMO2 and SUMO3 from CDCA8. Redox sensor that, when redistributed into nucleoplasm, can act as an effector to enhance HIF1A transcriptional activity by desumoylating EP300. Required for rRNA processing through deconjugation of SUMO2 and SUMO3 from nucleophosmin, NPM1. Plays a role in the regulation of sumoylation status of ZNF148. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.7 Publications

Activity regulationi

On oxidative stress, SENP3 degradation is blocked by inhibition of its ubiquitination, which stabilizes it as it accumulates in the nucleoplasm.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei465By similarity1
Active sitei482By similarity1
Active sitei532By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

BRENDAi3.4.22.B72 2681
ReactomeiR-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Protein family/group databases

MEROPSiC48.003

Names & Taxonomyi

Protein namesi
Recommended name:
Sentrin-specific protease 3 (EC:3.4.22.-)
Alternative name(s):
SUMO-1-specific protease 3
Sentrin/SUMO-specific protease SENP3
Gene namesi
Name:SENP3
Synonyms:SSP3, SUSP3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000161956.12
HGNCiHGNC:17862 SENP3
MIMi612844 gene
neXtProtiNX_Q9H4L4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi532C → S: Loss of enzymatic activity. 1 Publication1

Organism-specific databases

DisGeNETi26168
OpenTargetsiENSG00000161956
PharmGKBiPA134933213

Polymorphism and mutation databases

BioMutaiSENP3
DMDMi119370525

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001017211 – 574Sentrin-specific protease 3Add BLAST574

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei54PhosphoserineCombined sources1
Modified residuei73PhosphoserineBy similarity1
Modified residuei75PhosphoserineBy similarity1
Modified residuei169PhosphoserineBy similarity1
Modified residuei176PhosphothreonineBy similarity1
Modified residuei181PhosphoserineBy similarity1
Modified residuei188PhosphoserineBy similarity1
Modified residuei212PhosphoserineBy similarity1
Modified residuei232PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9H4L4
MaxQBiQ9H4L4
PaxDbiQ9H4L4
PeptideAtlasiQ9H4L4
PRIDEiQ9H4L4
ProteomicsDBi80851

PTM databases

iPTMnetiQ9H4L4
PhosphoSitePlusiQ9H4L4

Expressioni

Gene expression databases

BgeeiENSG00000161956 Expressed in 223 organ(s), highest expression level in anterior cingulate cortex
CleanExiHS_SENP3
ExpressionAtlasiQ9H4L4 baseline and differential
GenevisibleiQ9H4L4 HS

Organism-specific databases

HPAiHPA060290

Interactioni

Subunit structurei

Binds to SUMO1 and SUMO3 (By similarity). Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with EP300, NPM1 and CDCA8. Component of the 5FMC complex, at least composed of PELP1, LAS1L, TEX10, WDR18 and SENP3; the complex interacts with methylated CHTOP and ZNF148. Interacts with NOL9. Interacts with CCAR2.By similarity7 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi117594, 121 interactors
CORUMiQ9H4L4
DIPiDIP-47511N
IntActiQ9H4L4, 32 interactors
MINTiQ9H4L4
STRINGi9606.ENSP00000403712

Structurei

3D structure databases

ProteinModelPortaliQ9H4L4
SMRiQ9H4L4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni386 – 543ProteaseAdd BLAST158

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi125 – 128Nuclear localization signalSequence analysis4
Motifi153 – 159Nuclear localization signalSequence analysis7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi13 – 68Pro-richAdd BLAST56
Compositional biasi76 – 92Glu-richAdd BLAST17
Compositional biasi192 – 195Poly-Pro4

Sequence similaritiesi

Belongs to the peptidase C48 family.Curated

Phylogenomic databases

eggNOGiKOG0778 Eukaryota
COG5160 LUCA
GeneTreeiENSGT00530000062941
HOGENOMiHOG000154287
HOVERGENiHBG059450
InParanoidiQ9H4L4
KOiK08593
OrthoDBiEOG091G092N
PhylomeDBiQ9H4L4
TreeFamiTF316289

Family and domain databases

InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR003653 Peptidase_C48_C
IPR037945 SENP3
PANTHERiPTHR12606:SF16 PTHR12606:SF16, 1 hit
PfamiView protein in Pfam
PF02902 Peptidase_C48, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS50600 ULP_PROTEASE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.iShow all

Q9H4L4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKETIQGTGS WGPEPPGPGI PPAYSSPRRE RLRWPPPPKP RLKSGGGFGP
60 70 80 90 100
DPGSGTTVPA RRLPVPRPSF DASASEEEEE EEEEEDEDEE EEVAAWRLPP
110 120 130 140 150
RWSQLGTSQR PRPSRPTHRK TCSQRRRRAM RAFRMLLYSK STSLTFHWKL
160 170 180 190 200
WGRHRGRRRG LAHPKNHLSP QQGGATPQVP SPCCRFDSPR GPPPPRLGLL
210 220 230 240 250
GALMAEDGVR GSPPVPSGPP MEEDGLRWTP KSPLDPDSGL LSCTLPNGFG
260 270 280 290 300
GQSGPEGERS LAPPDASILI SNVCSIGDHV AQELFQGSDL GMAEEAERPG
310 320 330 340 350
EKAGQHSPLR EEHVTCVQSI LDEFLQTYGS LIPLSTDEVV EKLEDIFQQE
360 370 380 390 400
FSTPSRKGLV LQLIQSYQRM PGNAMVRGFR VAYKRHVLTM DDLGTLYGQN
410 420 430 440 450
WLNDQVMNMY GDLVMDTVPE KVHFFNSFFY DKLRTKGYDG VKRWTKNVDI
460 470 480 490 500
FNKELLLIPI HLEVHWSLIS VDVRRRTITY FDSQRTLNRR CPKHIAKYLQ
510 520 530 540 550
AEAVKKDRLD FHQGWKGYFK MNVARQNNDS DCGAFVLQYC KHLALSQPFS
560 570
FTQQDMPKLR RQIYKELCHC KLTV
Length:574
Mass (Da):65,010
Last modified:December 12, 2006 - v2
Checksum:iE495137EE7500741
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3QL36J3QL36_HUMAN
Sentrin-specific protease 3
SENP3
237Annotation score:
A0A087WV50A0A087WV50_HUMAN
Sentrin-specific protease 3
SENP3
177Annotation score:
J3KSF5J3KSF5_HUMAN
Sentrin-specific protease 3
SENP3
82Annotation score:
J3QRE4J3QRE4_HUMAN
Sentrin-specific protease 3
SENP3
110Annotation score:
A0A087WZ63A0A087WZ63_HUMAN
Sentrin-specific protease 3
SENP3
63Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30 – 31ER → DG in AAG33252 (PubMed:10806345).Curated2
Sequence conflicti60A → T in AAG33252 (PubMed:10806345).Curated1
Sequence conflicti65V → A in AAG33252 (PubMed:10806345).Curated1
Sequence conflicti113 – 114PS → AL in AAG33252 (PubMed:10806345).Curated2
Sequence conflicti117T → S in AAG33252 (PubMed:10806345).Curated1
Sequence conflicti134R → Q in AAG33252 (PubMed:10806345).Curated1
Sequence conflicti161L → S in AAH48306 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051544515W → R. Corresponds to variant dbSNP:rs9972914Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY008763 mRNA Translation: AAG33252.1
AF199459 mRNA Translation: AAL25652.1
AL050283 mRNA Translation: CAB43384.2
AL834294 mRNA Translation: CAD38967.1
BC048306 mRNA Translation: AAH48306.1
BC080658 mRNA Translation: AAH80658.1
CCDSiCCDS73958.1
PIRiT08759
RefSeqiNP_056485.2, NM_015670.5
UniGeneiHs.513926
Hs.733104

Genome annotation databases

EnsembliENST00000321337; ENSP00000314029; ENSG00000161956
ENST00000429205; ENSP00000403712; ENSG00000161956
GeneIDi26168
KEGGihsa:26168
UCSCiuc032esp.2 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY008763 mRNA Translation: AAG33252.1
AF199459 mRNA Translation: AAL25652.1
AL050283 mRNA Translation: CAB43384.2
AL834294 mRNA Translation: CAD38967.1
BC048306 mRNA Translation: AAH48306.1
BC080658 mRNA Translation: AAH80658.1
CCDSiCCDS73958.1
PIRiT08759
RefSeqiNP_056485.2, NM_015670.5
UniGeneiHs.513926
Hs.733104

3D structure databases

ProteinModelPortaliQ9H4L4
SMRiQ9H4L4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117594, 121 interactors
CORUMiQ9H4L4
DIPiDIP-47511N
IntActiQ9H4L4, 32 interactors
MINTiQ9H4L4
STRINGi9606.ENSP00000403712

Protein family/group databases

MEROPSiC48.003

PTM databases

iPTMnetiQ9H4L4
PhosphoSitePlusiQ9H4L4

Polymorphism and mutation databases

BioMutaiSENP3
DMDMi119370525

Proteomic databases

EPDiQ9H4L4
MaxQBiQ9H4L4
PaxDbiQ9H4L4
PeptideAtlasiQ9H4L4
PRIDEiQ9H4L4
ProteomicsDBi80851

Protocols and materials databases

DNASUi26168
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000321337; ENSP00000314029; ENSG00000161956
ENST00000429205; ENSP00000403712; ENSG00000161956
GeneIDi26168
KEGGihsa:26168
UCSCiuc032esp.2 human

Organism-specific databases

CTDi26168
DisGeNETi26168
EuPathDBiHostDB:ENSG00000161956.12
GeneCardsiSENP3
H-InvDBiHIX0013501
HGNCiHGNC:17862 SENP3
HPAiHPA060290
MIMi612844 gene
neXtProtiNX_Q9H4L4
OpenTargetsiENSG00000161956
PharmGKBiPA134933213
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0778 Eukaryota
COG5160 LUCA
GeneTreeiENSGT00530000062941
HOGENOMiHOG000154287
HOVERGENiHBG059450
InParanoidiQ9H4L4
KOiK08593
OrthoDBiEOG091G092N
PhylomeDBiQ9H4L4
TreeFamiTF316289

Enzyme and pathway databases

BRENDAi3.4.22.B72 2681
ReactomeiR-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Miscellaneous databases

ChiTaRSiSENP3 human
GeneWikiiSENP3
GenomeRNAii26168
PROiPR:Q9H4L4
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000161956 Expressed in 223 organ(s), highest expression level in anterior cingulate cortex
CleanExiHS_SENP3
ExpressionAtlasiQ9H4L4 baseline and differential
GenevisibleiQ9H4L4 HS

Family and domain databases

InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR003653 Peptidase_C48_C
IPR037945 SENP3
PANTHERiPTHR12606:SF16 PTHR12606:SF16, 1 hit
PfamiView protein in Pfam
PF02902 Peptidase_C48, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS50600 ULP_PROTEASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSENP3_HUMAN
AccessioniPrimary (citable) accession number: Q9H4L4
Secondary accession number(s): Q66K15
, Q86VS7, Q96PS4, Q9Y3W9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: December 12, 2006
Last modified: September 12, 2018
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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