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Protein

Rho-related GTP-binding protein RhoJ

Gene

RHOJ

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plasma membrane-associated small GTPase specifically involved in angiogenesis (PubMed:21628409, PubMed:24434213, PubMed:30158707). Required for endothelial cell migration during vascular development via its interaction with GLUL (PubMed:30158707). Elicits the formation of F-actin-rich structures, thereby regulating endothelial cell migration (PubMed:30158707).3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 36GTPBy similarity6
Nucleotide bindingi46 – 53GTPBy similarity8
Nucleotide bindingi75 – 79GTPBy similarity5
Nucleotide bindingi133 – 136GTPBy similarity4
Nucleotide bindingi177 – 178GTPBy similarity2

GO - Molecular functioni

  • GTPase activity Source: GO_Central
  • GTP binding Source: GO_Central
  • protein kinase binding Source: GO_Central

GO - Biological processi

Keywordsi

Biological processAngiogenesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-194840 Rho GTPase cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-related GTP-binding protein RhoJCurated
Alternative name(s):
Ras-like protein family member 7B
Tc10-like GTP-binding protein1 Publication
Gene namesi
Name:RHOJ
Synonyms:ARHJ, RASL7B, RHOI1 Publication, TCL1 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000126785.12
HGNCiHGNC:688 RHOJ
MIMi607653 gene
neXtProtiNX_Q9H4E5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi3C → A: Impaired localization to the plasma membrane. 1 Publication1
Mutagenesisi11C → A: Impaired localization to the plasma membrane. 1 Publication1
Mutagenesisi17 – 20DEKK → AAAA: Impaired localization to the plasma membrane. 1 Publication4
Mutagenesisi17 – 18DE → AA: Does not affect localization to the plasma membrane. 1 Publication2
Mutagenesisi19 – 20KK → AA: Impaired localization to the plasma membrane. 1 Publication2
Mutagenesisi30G → V: Causes constitutive activation. 1 Publication1
Mutagenesisi35T → N: Dominant negative mutant. 1 Publication1
Mutagenesisi79Q → L: Causes constitutive activation. 1 Publication1

Organism-specific databases

DisGeNETi57381
OpenTargetsiENSG00000126785
PharmGKBiPA24981

Polymorphism and mutation databases

BioMutaiRHOJ
DMDMi24418646

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001988691 – 211Rho-related GTP-binding protein RhoJAdd BLAST211
PropeptideiPRO_0000281220212 – 214Removed in mature formCurated3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi3S-palmitoyl cysteine1 Publication1
Lipidationi11S-palmitoyl cysteine1 Publication1
Modified residuei211Cysteine methyl esterBy similarity1
Lipidationi211S-farnesyl cysteineBy similarity1

Post-translational modificationi

Palmitoylated; regulates localization to the plasma membrane and may be mediated by GLUL.1 Publication

Keywords - PTMi

Lipoprotein, Methylation, Palmitate, Prenylation

Proteomic databases

EPDiQ9H4E5
MaxQBiQ9H4E5
PaxDbiQ9H4E5
PeptideAtlasiQ9H4E5
PRIDEiQ9H4E5
ProteomicsDBi80825
80826 [Q9H4E5-2]

PTM databases

iPTMnetiQ9H4E5
PhosphoSitePlusiQ9H4E5
SwissPalmiQ9H4E5

Expressioni

Tissue specificityi

Specifically expressed in endothelial cells in different tissues, such as brain, heart, lung and liver.1 Publication

Inductioni

Expression is regulated by the transcription factor ERG.1 Publication

Gene expression databases

BgeeiENSG00000126785 Expressed in 189 organ(s), highest expression level in tendon of biceps brachii
CleanExiHS_RHOJ
ExpressionAtlasiQ9H4E5 baseline and differential
GenevisibleiQ9H4E5 HS

Organism-specific databases

HPAiHPA003050

Interactioni

Subunit structurei

Interacts with the CRIB domains of proteins such as Pak1 and Was/Wasp (By similarity). Interacts with GLUL (PubMed:30158707).By similarity1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi121499, 20 interactors
IntActiQ9H4E5, 18 interactors
MINTiQ9H4E5
STRINGi9606.ENSP00000316729

Structurei

3D structure databases

ProteinModelPortaliQ9H4E5
SMRiQ9H4E5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi50 – 58Effector regionSequence analysis9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000118978
HOVERGENiHBG009351
InParanoidiQ9H4E5
KOiK07864
OMAiKKRCSEC
OrthoDBiEOG091G0KCM
PhylomeDBiQ9H4E5
TreeFamiTF101109

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9H4E5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNCKEGTDSS CGCRGNDEKK MLKCVVVGDG AVGKTCLLMS YANDAFPEEY
60 70 80 90 100
VPTVFDHYAV TVTVGGKQHL LGLYDTAGQE DYNQLRPLSY PNTDVFLICF
110 120 130 140 150
SVVNPASYHN VQEEWVPELK DCMPHVPYVL IGTQIDLRDD PKTLARLLYM
160 170 180 190 200
KEKPLTYEHG VKLAKAIGAQ CYLECSALTQ KGLKAVFDEA ILTIFHPKKK
210
KKRCSEGHSC CSII
Length:214
Mass (Da):23,821
Last modified:March 1, 2001 - v1
Checksum:iAAD2E2CED036F48C
GO
Isoform 2 (identifier: Q9H4E5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     113-134: Missing.

Note: No experimental confirmation available. Could be created by usage of an unusual splicing donor site.
Show »
Length:192
Mass (Da):21,255
Checksum:iF20844F6EC738489
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V4H1G3V4H1_HUMAN
Rho-related GTP-binding protein Rho...
RHOJ
153Annotation score:
G3V476G3V476_HUMAN
Rho-related GTP-binding protein Rho...
RHOJ
178Annotation score:

Sequence cautioni

The sequence BAB55013 differs from that shown. Reason: Frameshift at position 143.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3C → R in BAB55013 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007231113 – 134Missing in isoform 2. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ276567 mRNA Translation: CAC06611.1
AF309563 mRNA Translation: AAL09440.1
AK027278 mRNA Translation: BAB55013.1 Frameshift.
AK027351 mRNA Translation: BAB55055.1
AF498977 mRNA Translation: AAM21124.1
BC062575 mRNA Translation: AAH62575.1
CCDSiCCDS9757.1 [Q9H4E5-1]
RefSeqiNP_065714.1, NM_020663.4 [Q9H4E5-1]
UniGeneiHs.656339

Genome annotation databases

EnsembliENST00000316754; ENSP00000316729; ENSG00000126785 [Q9H4E5-1]
GeneIDi57381
KEGGihsa:57381
UCSCiuc001xgb.3 human [Q9H4E5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ276567 mRNA Translation: CAC06611.1
AF309563 mRNA Translation: AAL09440.1
AK027278 mRNA Translation: BAB55013.1 Frameshift.
AK027351 mRNA Translation: BAB55055.1
AF498977 mRNA Translation: AAM21124.1
BC062575 mRNA Translation: AAH62575.1
CCDSiCCDS9757.1 [Q9H4E5-1]
RefSeqiNP_065714.1, NM_020663.4 [Q9H4E5-1]
UniGeneiHs.656339

3D structure databases

ProteinModelPortaliQ9H4E5
SMRiQ9H4E5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121499, 20 interactors
IntActiQ9H4E5, 18 interactors
MINTiQ9H4E5
STRINGi9606.ENSP00000316729

PTM databases

iPTMnetiQ9H4E5
PhosphoSitePlusiQ9H4E5
SwissPalmiQ9H4E5

Polymorphism and mutation databases

BioMutaiRHOJ
DMDMi24418646

Proteomic databases

EPDiQ9H4E5
MaxQBiQ9H4E5
PaxDbiQ9H4E5
PeptideAtlasiQ9H4E5
PRIDEiQ9H4E5
ProteomicsDBi80825
80826 [Q9H4E5-2]

Protocols and materials databases

DNASUi57381
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000316754; ENSP00000316729; ENSG00000126785 [Q9H4E5-1]
GeneIDi57381
KEGGihsa:57381
UCSCiuc001xgb.3 human [Q9H4E5-1]

Organism-specific databases

CTDi57381
DisGeNETi57381
EuPathDBiHostDB:ENSG00000126785.12
GeneCardsiRHOJ
HGNCiHGNC:688 RHOJ
HPAiHPA003050
MIMi607653 gene
neXtProtiNX_Q9H4E5
OpenTargetsiENSG00000126785
PharmGKBiPA24981
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000118978
HOVERGENiHBG009351
InParanoidiQ9H4E5
KOiK07864
OMAiKKRCSEC
OrthoDBiEOG091G0KCM
PhylomeDBiQ9H4E5
TreeFamiTF101109

Enzyme and pathway databases

ReactomeiR-HSA-194840 Rho GTPase cycle

Miscellaneous databases

ChiTaRSiRHOJ human
GeneWikiiRHOJ
GenomeRNAii57381
PROiPR:Q9H4E5
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000126785 Expressed in 189 organ(s), highest expression level in tendon of biceps brachii
CleanExiHS_RHOJ
ExpressionAtlasiQ9H4E5 baseline and differential
GenevisibleiQ9H4E5 HS

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRHOJ_HUMAN
AccessioniPrimary (citable) accession number: Q9H4E5
Secondary accession number(s): Q96KC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: March 1, 2001
Last modified: November 7, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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