UniProtKB - Q9H4E5 (RHOJ_HUMAN)
Protein
Rho-related GTP-binding protein RhoJ
Gene
RHOJ
Organism
Homo sapiens (Human)
Status
Functioni
Plasma membrane-associated small GTPase specifically involved in angiogenesis (PubMed:21628409, PubMed:24434213, PubMed:30158707). Required for endothelial cell migration during vascular development via its interaction with GLUL (PubMed:30158707). Elicits the formation of F-actin-rich structures, thereby regulating endothelial cell migration (PubMed:30158707).3 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 31 – 36 | GTPBy similarity | 6 | |
Nucleotide bindingi | 46 – 53 | GTPBy similarity | 8 | |
Nucleotide bindingi | 75 – 79 | GTPBy similarity | 5 | |
Nucleotide bindingi | 133 – 136 | GTPBy similarity | 4 | |
Nucleotide bindingi | 177 – 178 | GTPBy similarity | 2 |
GO - Molecular functioni
- GTPase activity Source: UniProtKB
- GTP binding Source: UniProtKB
GO - Biological processi
- actin cytoskeleton organization Source: UniProtKB
- angiogenesis Source: UniProtKB-KW
- Cdc42 protein signal transduction Source: GO_Central
- cell migration Source: GO_Central
- cell projection assembly Source: GO_Central
- endocytosis Source: GO_Central
- positive regulation of cell migration involved in sprouting angiogenesis Source: BHF-UCL
- regulation of cell shape Source: Ensembl
- regulation of endothelial cell migration Source: UniProtKB
- regulation of small GTPase mediated signal transduction Source: Reactome
- regulation of sprouting angiogenesis Source: UniProtKB
- retina vasculature morphogenesis in camera-type eye Source: Ensembl
Keywordsi
Biological process | Angiogenesis |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9H4E5 |
Reactomei | R-HSA-194840, Rho GTPase cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Rho-related GTP-binding protein RhoJCuratedAlternative name(s): Ras-like protein family member 7B Tc10-like GTP-binding protein1 Publication |
Gene namesi | Name:RHOJ Synonyms:ARHJ, RASL7B, RHOI1 Publication, TCL1 Publication |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:688, RHOJ |
MIMi | 607653, gene |
neXtProti | NX_Q9H4E5 |
VEuPathDBi | HostDB:ENSG00000126785.12 |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Lipid-anchor 1 Publication; Cytoplasmic side Curated
Note: Localization to the plasma membrane is regulated by GLUL.1 Publication
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 3 | C → A: Impaired localization to the plasma membrane. 1 Publication | 1 | |
Mutagenesisi | 11 | C → A: Impaired localization to the plasma membrane. 1 Publication | 1 | |
Mutagenesisi | 17 – 20 | DEKK → AAAA: Impaired localization to the plasma membrane. 1 Publication | 4 | |
Mutagenesisi | 17 – 18 | DE → AA: Does not affect localization to the plasma membrane. 1 Publication | 2 | |
Mutagenesisi | 19 – 20 | KK → AA: Impaired localization to the plasma membrane. 1 Publication | 2 | |
Mutagenesisi | 30 | G → V: Causes constitutive activation. 1 Publication | 1 | |
Mutagenesisi | 35 | T → N: Dominant negative mutant. 1 Publication | 1 | |
Mutagenesisi | 79 | Q → L: Causes constitutive activation. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 57381 |
OpenTargetsi | ENSG00000126785 |
PharmGKBi | PA24981 |
Miscellaneous databases
Pharosi | Q9H4E5, Tbio |
Genetic variation databases
BioMutai | RHOJ |
DMDMi | 24418646 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000198869 | 1 – 211 | Rho-related GTP-binding protein RhoJAdd BLAST | 211 | |
PropeptideiPRO_0000281220 | 212 – 214 | Removed in mature formCurated | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 3 | S-palmitoyl cysteine1 Publication | 1 | |
Lipidationi | 11 | S-palmitoyl cysteine1 Publication | 1 | |
Modified residuei | 211 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 211 | S-farnesyl cysteineBy similarity | 1 |
Post-translational modificationi
Palmitoylated; regulates localization to the plasma membrane and may be mediated by GLUL.1 Publication
Keywords - PTMi
Lipoprotein, Methylation, Palmitate, PrenylationProteomic databases
jPOSTi | Q9H4E5 |
MassIVEi | Q9H4E5 |
MaxQBi | Q9H4E5 |
PaxDbi | Q9H4E5 |
PeptideAtlasi | Q9H4E5 |
PRIDEi | Q9H4E5 |
ProteomicsDBi | 80825 [Q9H4E5-1] 80826 [Q9H4E5-2] |
PTM databases
iPTMneti | Q9H4E5 |
PhosphoSitePlusi | Q9H4E5 |
SwissPalmi | Q9H4E5 |
Expressioni
Tissue specificityi
Specifically expressed in endothelial cells in different tissues, such as brain, heart, lung and liver.1 Publication
Inductioni
Expression is regulated by the transcription factor ERG.1 Publication
Gene expression databases
Bgeei | ENSG00000126785, Expressed in sural nerve and 201 other tissues |
ExpressionAtlasi | Q9H4E5, baseline and differential |
Genevisiblei | Q9H4E5, HS |
Organism-specific databases
HPAi | ENSG00000126785, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with the CRIB domains of proteins such as Pak1 and Was/Wasp (By similarity).
Interacts with GLUL (PubMed:30158707).
By similarity1 PublicationBinary interactionsi
Hide detailsQ9H4E5
Isoform 1 [Q9H4E5-1]
With | #Exp. | IntAct |
---|---|---|
GLUL [P15104] | 2 | EBI-20738368,EBI-746653 |
Protein-protein interaction databases
BioGRIDi | 121499, 24 interactors |
IntActi | Q9H4E5, 27 interactors |
MINTi | Q9H4E5 |
STRINGi | 9606.ENSP00000316729 |
Miscellaneous databases
RNActi | Q9H4E5, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 50 – 58 | Effector regionSequence analysis | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0393, Eukaryota |
GeneTreei | ENSGT00940000159098 |
HOGENOMi | CLU_041217_21_3_1 |
InParanoidi | Q9H4E5 |
OMAi | TKKMLKC |
PhylomeDBi | Q9H4E5 |
TreeFami | TF101109 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR003578, Small_GTPase_Rho |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51420, RHO, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9H4E5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNCKEGTDSS CGCRGNDEKK MLKCVVVGDG AVGKTCLLMS YANDAFPEEY
60 70 80 90 100
VPTVFDHYAV TVTVGGKQHL LGLYDTAGQE DYNQLRPLSY PNTDVFLICF
110 120 130 140 150
SVVNPASYHN VQEEWVPELK DCMPHVPYVL IGTQIDLRDD PKTLARLLYM
160 170 180 190 200
KEKPLTYEHG VKLAKAIGAQ CYLECSALTQ KGLKAVFDEA ILTIFHPKKK
210
KKRCSEGHSC CSII
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V4H1 | G3V4H1_HUMAN | Rho-related GTP-binding protein Rho... | RHOJ | 153 | Annotation score: | ||
G3V476 | G3V476_HUMAN | Rho-related GTP-binding protein Rho... | RHOJ | 178 | Annotation score: |
Sequence cautioni
The sequence BAB55013 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 3 | C → R in BAB55013 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_007231 | 113 – 134 | Missing in isoform 2. 1 PublicationAdd BLAST | 22 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ276567 mRNA Translation: CAC06611.1 AF309563 mRNA Translation: AAL09440.1 AK027278 mRNA Translation: BAB55013.1 Frameshift. AK027351 mRNA Translation: BAB55055.1 AF498977 mRNA Translation: AAM21124.1 BC062575 mRNA Translation: AAH62575.1 |
CCDSi | CCDS9757.1 [Q9H4E5-1] |
RefSeqi | NP_065714.1, NM_020663.4 [Q9H4E5-1] |
Genome annotation databases
Ensembli | ENST00000316754; ENSP00000316729; ENSG00000126785 [Q9H4E5-1] |
GeneIDi | 57381 |
KEGGi | hsa:57381 |
UCSCi | uc001xgb.3, human [Q9H4E5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ276567 mRNA Translation: CAC06611.1 AF309563 mRNA Translation: AAL09440.1 AK027278 mRNA Translation: BAB55013.1 Frameshift. AK027351 mRNA Translation: BAB55055.1 AF498977 mRNA Translation: AAM21124.1 BC062575 mRNA Translation: AAH62575.1 |
CCDSi | CCDS9757.1 [Q9H4E5-1] |
RefSeqi | NP_065714.1, NM_020663.4 [Q9H4E5-1] |
3D structure databases
SMRi | Q9H4E5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 121499, 24 interactors |
IntActi | Q9H4E5, 27 interactors |
MINTi | Q9H4E5 |
STRINGi | 9606.ENSP00000316729 |
PTM databases
iPTMneti | Q9H4E5 |
PhosphoSitePlusi | Q9H4E5 |
SwissPalmi | Q9H4E5 |
Genetic variation databases
BioMutai | RHOJ |
DMDMi | 24418646 |
Proteomic databases
jPOSTi | Q9H4E5 |
MassIVEi | Q9H4E5 |
MaxQBi | Q9H4E5 |
PaxDbi | Q9H4E5 |
PeptideAtlasi | Q9H4E5 |
PRIDEi | Q9H4E5 |
ProteomicsDBi | 80825 [Q9H4E5-1] 80826 [Q9H4E5-2] |
Protocols and materials databases
Antibodypediai | 160, 330 antibodies |
DNASUi | 57381 |
Genome annotation databases
Ensembli | ENST00000316754; ENSP00000316729; ENSG00000126785 [Q9H4E5-1] |
GeneIDi | 57381 |
KEGGi | hsa:57381 |
UCSCi | uc001xgb.3, human [Q9H4E5-1] |
Organism-specific databases
CTDi | 57381 |
DisGeNETi | 57381 |
GeneCardsi | RHOJ |
HGNCi | HGNC:688, RHOJ |
HPAi | ENSG00000126785, Low tissue specificity |
MIMi | 607653, gene |
neXtProti | NX_Q9H4E5 |
OpenTargetsi | ENSG00000126785 |
PharmGKBi | PA24981 |
VEuPathDBi | HostDB:ENSG00000126785.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0393, Eukaryota |
GeneTreei | ENSGT00940000159098 |
HOGENOMi | CLU_041217_21_3_1 |
InParanoidi | Q9H4E5 |
OMAi | TKKMLKC |
PhylomeDBi | Q9H4E5 |
TreeFami | TF101109 |
Enzyme and pathway databases
PathwayCommonsi | Q9H4E5 |
Reactomei | R-HSA-194840, Rho GTPase cycle |
Miscellaneous databases
BioGRID-ORCSi | 57381, 4 hits in 987 CRISPR screens |
ChiTaRSi | RHOJ, human |
GeneWikii | RHOJ |
GenomeRNAii | 57381 |
Pharosi | Q9H4E5, Tbio |
PROi | PR:Q9H4E5 |
RNActi | Q9H4E5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000126785, Expressed in sural nerve and 201 other tissues |
ExpressionAtlasi | Q9H4E5, baseline and differential |
Genevisiblei | Q9H4E5, HS |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR003578, Small_GTPase_Rho |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51420, RHO, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RHOJ_HUMAN | |
Accessioni | Q9H4E5Primary (citable) accession number: Q9H4E5 Secondary accession number(s): Q96KC1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2002 |
Last sequence update: | March 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families