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Protein

Aminopeptidase B

Gene

RNPEP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Exopeptidase which selectively removes arginine and/or lysine residues from the N-terminus of several peptide substrates including Arg0-Leu-enkephalin, Arg0-Met-enkephalin and Arg(-1)-Lys0-somatostatin-14. Can hydrolyze leukotriene A4 (LTA-4) into leukotriene B4 (LTB-4) (By similarity).By similarity

Catalytic activityi

Release of N-terminal Arg and Lys from oligopeptides when P1' is not Pro. Also acts on arylamides of Arg and Lys.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi325Zinc; catalyticPROSITE-ProRule annotation1
Active sitei326Proton acceptorPROSITE-ProRule annotation1
Metal bindingi329Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi348Zinc; catalyticPROSITE-ProRule annotation1
Sitei414Transition state stabilizerBy similarity1

GO - Molecular functioni

  • aminopeptidase activity Source: UniProtKB
  • epoxide hydrolase activity Source: UniProtKB
  • metalloexopeptidase activity Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • negative regulation of blood pressure Source: GO_Central
  • proteolysis Source: UniProtKB

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM01.014

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase B (EC:3.4.11.6)
Short name:
AP-B
Alternative name(s):
Arginine aminopeptidase
Arginyl aminopeptidase
Gene namesi
Name:RNPEP
Synonyms:APB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000176393.10
HGNCiHGNC:10078 RNPEP
MIMi602675 gene
neXtProtiNX_Q9H4A4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi6051
OpenTargetsiENSG00000176393
PharmGKBiPA34451

Chemistry databases

ChEMBLiCHEMBL2432

Polymorphism and mutation databases

BioMutaiRNPEP
DMDMi20137480

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000950882 – 650Aminopeptidase BAdd BLAST649

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei7PhosphoserineCombined sources1
Modified residuei446N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9H4A4
MaxQBiQ9H4A4
PaxDbiQ9H4A4
PeptideAtlasiQ9H4A4
PRIDEiQ9H4A4
ProteomicsDBi80809
TopDownProteomicsiQ9H4A4

PTM databases

iPTMnetiQ9H4A4
PhosphoSitePlusiQ9H4A4
SwissPalmiQ9H4A4

Expressioni

Gene expression databases

BgeeiENSG00000176393 Expressed in 94 organ(s), highest expression level in mucosa of transverse colon
CleanExiHS_RNPEP
ExpressionAtlasiQ9H4A4 baseline and differential
GenevisibleiQ9H4A4 HS

Organism-specific databases

HPAiHPA036074
HPA036075
HPA061487

Interactioni

Protein-protein interaction databases

BioGridi111978, 7 interactors
IntActiQ9H4A4, 2 interactors
MINTiQ9H4A4
STRINGi9606.ENSP00000295640

Chemistry databases

BindingDBiQ9H4A4

Structurei

3D structure databases

ProteinModelPortaliQ9H4A4
SMRiQ9H4A4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni298 – 302Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Phylogenomic databases

eggNOGiKOG1047 Eukaryota
COG0308 LUCA
GeneTreeiENSGT00530000063003
HOVERGENiHBG001274
InParanoidiQ9H4A4
KOiK01260
OMAiFEMEKPI
OrthoDBiEOG091G02UX
PhylomeDBiQ9H4A4
TreeFamiTF300758

Family and domain databases

CDDicd09599 M1_LTA4H, 1 hit
Gene3Di1.10.390.10, 1 hit
1.25.40.320, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR038502 M1_LTA-4_hydro/amino_C_sf
IPR034015 M1_LTA4H
IPR015571 Pept_M1_aminopeptidase-B
IPR001930 Peptidase_M1
IPR015211 Peptidase_M1_C
IPR014782 Peptidase_M1_N
IPR027268 Peptidase_M4/M1_CTD_sf
PANTHERiPTHR11533 PTHR11533, 1 hit
PTHR11533:SF153 PTHR11533:SF153, 1 hit
PfamiView protein in Pfam
PF09127 Leuk-A4-hydro_C, 1 hit
PF01433 Peptidase_M1, 1 hit
PRINTSiPR00756 ALADIPTASE
SMARTiView protein in SMART
SM01263 Leuk-A4-hydro_C, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

Q9H4A4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASGEHSPGS GAARRPLHSA QAVDVASASN FRAFELLHLH LDLRAEFGPP
60 70 80 90 100
GPGAGSRGLS GTAVLDLRCL EPEGAAELRL DSHPCLEVTA AALRRERPGS
110 120 130 140 150
EEPPAEPVSF YTQPFSHYGQ ALCVSFPQPC RAAERLQVLL TYRVGEGPGV
160 170 180 190 200
CWLAPEQTAG KKKPFVYTQG QAVLNRAFFP CFDTPAVKYK YSALIEVPDG
210 220 230 240 250
FTAVMSASTW EKRGPNKFFF QMCQPIPSYL IALAIGDLVS AEVGPRSRVW
260 270 280 290 300
AEPCLIDAAK EEYNGVIEEF LATGEKLFGP YVWGRYDLLF MPPSFPFGGM
310 320 330 340 350
ENPCLTFVTP CLLAGDRSLA DVIIHEISHS WFGNLVTNAN WGEFWLNEGF
360 370 380 390 400
TMYAQRRIST ILFGAAYTCL EAATGRALLR QHMDITGEEN PLNKLRVKIE
410 420 430 440 450
PGVDPDDTYN ETPYEKGFCF VSYLAHLVGD QDQFDSFLKA YVHEFKFRSI
460 470 480 490 500
LADDFLDFYL EYFPELKKKR VDIIPGFEFD RWLNTPGWPP YLPDLSPGDS
510 520 530 540 550
LMKPAEELAQ LWAAEELDMK AIEAVAISPW KTYQLVYFLD KILQKSPLPP
560 570 580 590 600
GNVKKLGDTY PSISNARNAE LRLRWGQIVL KNDHQEDFWK VKEFLHNQGK
610 620 630 640 650
QKYTLPLYHA MMGGSEVAQT LAKETFASTA SQLHSNVVNY VQQIVAPKGS
Length:650
Mass (Da):72,596
Last modified:March 27, 2002 - v2
Checksum:i4C04FE09689F2487
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JMZ3C9JMZ3_HUMAN
Aminopeptidase B
RNPEP
344Annotation score:
A6NKB8A6NKB8_HUMAN
Aminopeptidase B
RNPEP
611Annotation score:
H7C2T3H7C2T3_HUMAN
Aminopeptidase B
RNPEP
168Annotation score:
A0A087WU27A0A087WU27_HUMAN
Aminopeptidase B
RNPEP
171Annotation score:
A0A087WUS4A0A087WUS4_HUMAN
Aminopeptidase B
RNPEP
151Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2A → V in CAC14047 (Ref. 2) Curated1
Sequence conflicti11 – 14GAAR → ARPGGRCTPRRL in CAC12957 (Ref. 1) Curated4
Sequence conflicti149G → R in CAC12957 (Ref. 1) Curated1
Sequence conflicti153L → V in CAC12957 (Ref. 1) Curated1
Sequence conflicti208 – 210STW → RPG in CAC12957 (Ref. 1) Curated3
Sequence conflicti262Missing in CAC12957 (Ref. 1) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051566579V → I. Corresponds to variant dbSNP:rs3820439Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242586 mRNA Translation: CAC12957.1
AJ296161 mRNA Translation: CAC14047.1
BC001064 mRNA Translation: AAH01064.1
BC012166 mRNA Translation: AAH12166.1
AL390139 mRNA Translation: CAB99087.1
CCDSiCCDS1418.1
PIRiT51870
RefSeqiNP_001306111.1, NM_001319182.1
NP_001306112.1, NM_001319183.1
NP_001306113.1, NM_001319184.1
NP_064601.3, NM_020216.3
XP_005245478.1, XM_005245421.1
XP_016857511.1, XM_017002022.1
UniGeneiHs.497391

Genome annotation databases

EnsembliENST00000295640; ENSP00000295640; ENSG00000176393
GeneIDi6051
KEGGihsa:6051
UCSCiuc001gxd.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242586 mRNA Translation: CAC12957.1
AJ296161 mRNA Translation: CAC14047.1
BC001064 mRNA Translation: AAH01064.1
BC012166 mRNA Translation: AAH12166.1
AL390139 mRNA Translation: CAB99087.1
CCDSiCCDS1418.1
PIRiT51870
RefSeqiNP_001306111.1, NM_001319182.1
NP_001306112.1, NM_001319183.1
NP_001306113.1, NM_001319184.1
NP_064601.3, NM_020216.3
XP_005245478.1, XM_005245421.1
XP_016857511.1, XM_017002022.1
UniGeneiHs.497391

3D structure databases

ProteinModelPortaliQ9H4A4
SMRiQ9H4A4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111978, 7 interactors
IntActiQ9H4A4, 2 interactors
MINTiQ9H4A4
STRINGi9606.ENSP00000295640

Chemistry databases

BindingDBiQ9H4A4
ChEMBLiCHEMBL2432

Protein family/group databases

MEROPSiM01.014

PTM databases

iPTMnetiQ9H4A4
PhosphoSitePlusiQ9H4A4
SwissPalmiQ9H4A4

Polymorphism and mutation databases

BioMutaiRNPEP
DMDMi20137480

Proteomic databases

EPDiQ9H4A4
MaxQBiQ9H4A4
PaxDbiQ9H4A4
PeptideAtlasiQ9H4A4
PRIDEiQ9H4A4
ProteomicsDBi80809
TopDownProteomicsiQ9H4A4

Protocols and materials databases

DNASUi6051
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000295640; ENSP00000295640; ENSG00000176393
GeneIDi6051
KEGGihsa:6051
UCSCiuc001gxd.4 human

Organism-specific databases

CTDi6051
DisGeNETi6051
EuPathDBiHostDB:ENSG00000176393.10
GeneCardsiRNPEP
H-InvDBiHIX0023896
HGNCiHGNC:10078 RNPEP
HPAiHPA036074
HPA036075
HPA061487
MIMi602675 gene
neXtProtiNX_Q9H4A4
OpenTargetsiENSG00000176393
PharmGKBiPA34451
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1047 Eukaryota
COG0308 LUCA
GeneTreeiENSGT00530000063003
HOVERGENiHBG001274
InParanoidiQ9H4A4
KOiK01260
OMAiFEMEKPI
OrthoDBiEOG091G02UX
PhylomeDBiQ9H4A4
TreeFamiTF300758

Miscellaneous databases

ChiTaRSiRNPEP human
GeneWikiiRNPEP
GenomeRNAii6051
PROiPR:Q9H4A4
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000176393 Expressed in 94 organ(s), highest expression level in mucosa of transverse colon
CleanExiHS_RNPEP
ExpressionAtlasiQ9H4A4 baseline and differential
GenevisibleiQ9H4A4 HS

Family and domain databases

CDDicd09599 M1_LTA4H, 1 hit
Gene3Di1.10.390.10, 1 hit
1.25.40.320, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR038502 M1_LTA-4_hydro/amino_C_sf
IPR034015 M1_LTA4H
IPR015571 Pept_M1_aminopeptidase-B
IPR001930 Peptidase_M1
IPR015211 Peptidase_M1_C
IPR014782 Peptidase_M1_N
IPR027268 Peptidase_M4/M1_CTD_sf
PANTHERiPTHR11533 PTHR11533, 1 hit
PTHR11533:SF153 PTHR11533:SF153, 1 hit
PfamiView protein in Pfam
PF09127 Leuk-A4-hydro_C, 1 hit
PF01433 Peptidase_M1, 1 hit
PRINTSiPR00756 ALADIPTASE
SMARTiView protein in SMART
SM01263 Leuk-A4-hydro_C, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMPB_HUMAN
AccessioniPrimary (citable) accession number: Q9H4A4
Secondary accession number(s): Q9BVM9, Q9H1D4, Q9NPT7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: November 7, 2018
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Peptidase families
    Classification of peptidase families and list of entries
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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