UniProtKB - Q9H477 (RBSK_HUMAN)
Protein
Ribokinase
Gene
RBKS
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.UniRule annotation1 Publication
Catalytic activityi
- EC:2.7.1.15UniRule annotation1 Publication
Cofactori
Mg2+UniRule annotationNote: Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.UniRule annotation
Activity regulationi
Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity (By similarity). Competitively inhibited by phosphonoacetic acid, etidronate, 2-carboxethylphosphonic acid, N-(phosphonomethyl)glycine, N-(phosphonomethyl)iminodiacetic acid and clodronate (PubMed:17585908).UniRule annotation1 Publication
Kineticsi
- KM=2.17 mM for ribose (in the presence of 10 mM inorganic phosphate)1 Publication
- KM=3.39 mM for ribose (in the presence of 5 mM inorganic phosphate)1 Publication
- KM=6.62 mM for ribose (in the presence of 2 mM inorganic phosphate)1 Publication
- KM=30.43 mM for ribose (in the presence of 1 mM inorganic phosphate)1 Publication
- KM=0.07 mM for ATP (in the presence of 5 mM inorganic phosphate)1 Publication
- KM=0.08 mM for ATP (in the presence of 2 mM inorganic phosphate)1 Publication
- KM=0.10 mM for ATP (in the presence of 1 mM inorganic phosphate)1 Publication
: D-ribose degradation Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose.UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- no protein annotated in this organism
- Ribokinase (RBKS)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose, the pathway D-ribose degradation and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 154 | SubstrateUniRule annotationBy similarity | 1 | |
Binding sitei | 199 | ATPUniRule annotation1 Publication | 1 | |
Binding sitei | 256 | ATPUniRule annotation1 Publication | 1 | |
Metal bindingi | 263 | PotassiumUniRule annotation1 Publication | 1 | |
Metal bindingi | 265 | Potassium; via carbonyl oxygenUniRule annotationBy similarity | 1 | |
Active sitei | 269 | Proton acceptorUniRule annotationBy similarity | 1 | |
Binding sitei | 269 | SubstrateUniRule annotationBy similarity | 1 | |
Binding sitei | 295 | ATPUniRule annotation1 Publication | 1 | |
Metal bindingi | 301 | Potassium; via carbonyl oxygenUniRule annotation1 Publication | 1 | |
Metal bindingi | 304 | Potassium; via carbonyl oxygenUniRule annotation1 Publication | 1 | |
Metal bindingi | 306 | Potassium; via carbonyl oxygenUniRule annotationBy similarity | 1 | |
Metal bindingi | 310 | PotassiumUniRule annotation1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 235 – 240 | ATPUniRule annotation1 Publication | 6 | |
Nucleotide bindingi | 268 – 269 | ATPUniRule annotation1 Publication | 2 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- identical protein binding Source: IntAct
- metal ion binding Source: UniProtKB-KW
- ribokinase activity Source: Reactome
GO - Biological processi
- D-ribose catabolic process Source: UniProtKB-UniRule
- pentose-phosphate shunt Source: Reactome
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Carbohydrate metabolism |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium |
Enzyme and pathway databases
PathwayCommonsi | Q9H477 |
Reactomei | R-HSA-71336, Pentose phosphate pathway |
SABIO-RKi | Q9H477 |
UniPathwayi | UPA00916;UER00889 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribokinase1 PublicationUniRule annotation (EC:2.7.1.15UniRule annotation1 Publication)Short name: RK1 PublicationUniRule annotation |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:30325, RBKS |
MIMi | 611132, gene |
neXtProti | NX_Q9H477 |
VEuPathDBi | HostDB:ENSG00000171174.13 |
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 64080 |
OpenTargetsi | ENSG00000171174 |
PharmGKBi | PA134951602 |
Miscellaneous databases
Pharosi | Q9H477, Tbio |
Genetic variation databases
BioMutai | RBKS |
DMDMi | 20139730 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080092 | 1 – 322 | RibokinaseAdd BLAST | 322 |
Proteomic databases
EPDi | Q9H477 |
jPOSTi | Q9H477 |
MassIVEi | Q9H477 |
MaxQBi | Q9H477 |
PaxDbi | Q9H477 |
PeptideAtlasi | Q9H477 |
PRIDEi | Q9H477 |
ProteomicsDBi | 5255 80790 [Q9H477-1] |
PTM databases
iPTMneti | Q9H477 |
PhosphoSitePlusi | Q9H477 |
Expressioni
Gene expression databases
Bgeei | ENSG00000171174, Expressed in duodenum and 198 other tissues |
ExpressionAtlasi | Q9H477, baseline and differential |
Genevisiblei | Q9H477, HS |
Organism-specific databases
HPAi | ENSG00000171174, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationBy similarityBinary interactionsi
Hide detailsQ9H477
With | #Exp. | IntAct |
---|---|---|
ACD [Q96AP0] | 2 | EBI-10982959,EBI-717666 |
itself | 4 | EBI-10982959,EBI-10982959 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 122046, 12 interactors |
IntActi | Q9H477, 8 interactors |
MINTi | Q9H477 |
STRINGi | 9606.ENSP00000306817 |
Miscellaneous databases
RNActi | Q9H477, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9H477 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9H477 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 25 – 27 | Substrate bindingUniRule annotationBy similarity | 3 | |
Regioni | 53 – 57 | Substrate bindingUniRule annotationBy similarity | 5 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2855, Eukaryota |
GeneTreei | ENSGT00390000005743 |
HOGENOMi | CLU_027634_2_3_1 |
InParanoidi | Q9H477 |
OMAi | DIVLIQQ |
OrthoDBi | 918144at2759 |
PhylomeDBi | Q9H477 |
TreeFami | TF105770 |
Family and domain databases
CDDi | cd01174, ribokinase, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
HAMAPi | MF_01987, Ribokinase, 1 hit |
InterProi | View protein in InterPro IPR002173, Carboh/pur_kinase_PfkB_CS IPR011877, D_ribokin IPR011611, PfkB_dom IPR002139, Ribo/fructo_kinase IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF00294, PfkB, 1 hit |
PRINTSi | PR00990, RIBOKINASE |
SUPFAMi | SSF53613, SSF53613, 1 hit |
TIGRFAMsi | TIGR02152, D_ribokin_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00584, PFKB_KINASES_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9H477-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAASGEPQRQ WQEEVAAVVV VGSCMTDLVS LTSRLPKTGE TIHGHKFFIG
60 70 80 90 100
FGGKGANQCV QAARLGAMTS MVCKVGKDSF GNDYIENLKQ NDISTEFTYQ
110 120 130 140 150
TKDAATGTAS IIVNNEGQNI IVIVAGANLL LNTEDLRAAA NVISRAKVMV
160 170 180 190 200
CQLEITPATS LEALTMARRS GVKTLFNPAP AIADLDPQFY TLSDVFCCNE
210 220 230 240 250
SEAEILTGLT VGSAADAGEA ALVLLKRGCQ VVIITLGAEG CVVLSQTEPE
260 270 280 290 300
PKHIPTEKVK AVDTTGAGDS FVGALAFYLA YYPNLSLEDM LNRSNFIAAV
310 320
SVQAAGTQSS YPYKKDLPLT LF
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH7C091 | H7C091_HUMAN | Ribokinase | RBKS | 147 | Annotation score: | ||
E7EQ18 | E7EQ18_HUMAN | Ribokinase | RBKS | 63 | Annotation score: | ||
E9PBU0 | E9PBU0_HUMAN | Ribokinase | RBKS | 36 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 101 | T → A in AAT64917 (Ref. 2) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054380 | 266 – 322 | GAGDS…PLTLF → CRPGSRPKSEAASVKKQKHY K in isoform 2. 1 PublicationAdd BLAST | 57 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ404857 mRNA Translation: CAC12877.1 AY643715 mRNA Translation: AAT64917.1 AK300989 mRNA Translation: BAG62608.1 AC021171 Genomic DNA No translation available. AC110084 Genomic DNA No translation available. BC017425 mRNA Translation: AAH17425.1 |
CCDSi | CCDS1762.1 [Q9H477-1] |
RefSeqi | NP_001274509.1, NM_001287580.1 NP_071411.1, NM_022128.2 [Q9H477-1] |
Genome annotation databases
Ensembli | ENST00000302188; ENSP00000306817; ENSG00000171174 [Q9H477-1] |
GeneIDi | 64080 |
KEGGi | hsa:64080 |
UCSCi | uc002rlo.3, human [Q9H477-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ404857 mRNA Translation: CAC12877.1 AY643715 mRNA Translation: AAT64917.1 AK300989 mRNA Translation: BAG62608.1 AC021171 Genomic DNA No translation available. AC110084 Genomic DNA No translation available. BC017425 mRNA Translation: AAH17425.1 |
CCDSi | CCDS1762.1 [Q9H477-1] |
RefSeqi | NP_001274509.1, NM_001287580.1 NP_071411.1, NM_022128.2 [Q9H477-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2FV7 | X-ray | 2.10 | A/B | 11-322 | [»] | |
5BYC | X-ray | 1.95 | A/B | 1-322 | [»] | |
5BYD | X-ray | 2.10 | A/B | 1-322 | [»] | |
5BYE | X-ray | 1.75 | A/B | 1-322 | [»] | |
5BYF | X-ray | 2.00 | A/B | 1-322 | [»] | |
5C3Y | X-ray | 2.60 | A/B/C/D/E/F/G/H/I/J/K/L | 1-322 | [»] | |
5C3Z | X-ray | 1.90 | A/B | 1-322 | [»] | |
5C40 | X-ray | 1.50 | A/B | 1-322 | [»] | |
5C41 | X-ray | 1.95 | A/B/C/D | 1-322 | [»] | |
SMRi | Q9H477 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 122046, 12 interactors |
IntActi | Q9H477, 8 interactors |
MINTi | Q9H477 |
STRINGi | 9606.ENSP00000306817 |
PTM databases
iPTMneti | Q9H477 |
PhosphoSitePlusi | Q9H477 |
Genetic variation databases
BioMutai | RBKS |
DMDMi | 20139730 |
Proteomic databases
EPDi | Q9H477 |
jPOSTi | Q9H477 |
MassIVEi | Q9H477 |
MaxQBi | Q9H477 |
PaxDbi | Q9H477 |
PeptideAtlasi | Q9H477 |
PRIDEi | Q9H477 |
ProteomicsDBi | 5255 80790 [Q9H477-1] |
Protocols and materials databases
Antibodypediai | 13898, 134 antibodies |
DNASUi | 64080 |
Genome annotation databases
Ensembli | ENST00000302188; ENSP00000306817; ENSG00000171174 [Q9H477-1] |
GeneIDi | 64080 |
KEGGi | hsa:64080 |
UCSCi | uc002rlo.3, human [Q9H477-1] |
Organism-specific databases
CTDi | 64080 |
DisGeNETi | 64080 |
GeneCardsi | RBKS |
HGNCi | HGNC:30325, RBKS |
HPAi | ENSG00000171174, Low tissue specificity |
MIMi | 611132, gene |
neXtProti | NX_Q9H477 |
OpenTargetsi | ENSG00000171174 |
PharmGKBi | PA134951602 |
VEuPathDBi | HostDB:ENSG00000171174.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2855, Eukaryota |
GeneTreei | ENSGT00390000005743 |
HOGENOMi | CLU_027634_2_3_1 |
InParanoidi | Q9H477 |
OMAi | DIVLIQQ |
OrthoDBi | 918144at2759 |
PhylomeDBi | Q9H477 |
TreeFami | TF105770 |
Enzyme and pathway databases
UniPathwayi | UPA00916;UER00889 |
PathwayCommonsi | Q9H477 |
Reactomei | R-HSA-71336, Pentose phosphate pathway |
SABIO-RKi | Q9H477 |
Miscellaneous databases
BioGRID-ORCSi | 64080, 3 hits in 870 CRISPR screens |
ChiTaRSi | RBKS, human |
EvolutionaryTracei | Q9H477 |
GenomeRNAii | 64080 |
Pharosi | Q9H477, Tbio |
PROi | PR:Q9H477 |
RNActi | Q9H477, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000171174, Expressed in duodenum and 198 other tissues |
ExpressionAtlasi | Q9H477, baseline and differential |
Genevisiblei | Q9H477, HS |
Family and domain databases
CDDi | cd01174, ribokinase, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
HAMAPi | MF_01987, Ribokinase, 1 hit |
InterProi | View protein in InterPro IPR002173, Carboh/pur_kinase_PfkB_CS IPR011877, D_ribokin IPR011611, PfkB_dom IPR002139, Ribo/fructo_kinase IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF00294, PfkB, 1 hit |
PRINTSi | PR00990, RIBOKINASE |
SUPFAMi | SSF53613, SSF53613, 1 hit |
TIGRFAMsi | TIGR02152, D_ribokin_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00584, PFKB_KINASES_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RBSK_HUMAN | |
Accessioni | Q9H477Primary (citable) accession number: Q9H477 Secondary accession number(s): A9UK04, B4DV96 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 23, 2002 |
Last sequence update: | March 1, 2001 | |
Last modified: | February 10, 2021 | |
This is version 165 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM