UniProtKB - Q9H444 (CHM4B_HUMAN)
Charged multivesicular body protein 4b
CHMP4B
Functioni
Miscellaneous
GO - Molecular functioni
- cadherin binding Source: BHF-UCL
- identical protein binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
GO - Biological processi
- autophagy Source: ParkinsonsUK-UCL
- endosomal transport Source: Reactome
- exit from mitosis Source: UniProtKB
- late endosome to vacuole transport via multivesicular body sorting pathway Source: GO_Central
- macroautophagy Source: ParkinsonsUK-UCL
- maintenance of lens transparency Source: UniProtKB
- membrane fission Source: UniProtKB
- midbody abscission Source: UniProtKB
- mitotic cytokinesis Source: UniProtKB
- mitotic metaphase plate congression Source: UniProtKB
- multivesicular body assembly Source: ParkinsonsUK-UCL
- negative regulation of autophagosome assembly Source: UniProtKB
- negative regulation of cell death Source: UniProtKB
- negative regulation of neuron death Source: UniProtKB
- nuclear envelope reassembly Source: UniProtKB
- nucleus organization Source: UniProtKB
- positive regulation of viral release from host cell Source: UniProtKB
- posttranslational protein targeting to endoplasmic reticulum membrane Source: UniProtKB
- protein polymerization Source: UniProtKB
- regulation of centrosome duplication Source: UniProtKB
- regulation of mitotic spindle assembly Source: UniProtKB
- regulation of viral process Source: UniProtKB
- ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway Source: UniProtKB
- vesicle budding from membrane Source: GO_Central
- viral budding Source: UniProtKB
- viral budding via host ESCRT complex Source: UniProtKB
- viral life cycle Source: Reactome
Keywordsi
Biological process | Host-virus interaction, Protein transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q9H444 |
Reactomei | R-HSA-162588, Budding and maturation of HIV virion R-HSA-1632852, Macroautophagy R-HSA-917729, Endosomal Sorting Complex Required For Transport (ESCRT) R-HSA-9610379, HCMV Late Events R-HSA-9615710, Late endosomal microautophagy R-HSA-9668328, Sealing of the nuclear envelope (NE) by ESCRT-III R-HSA-9679504, Translation of Replicase and Assembly of the Replication Transcription Complex |
Names & Taxonomyi
Protein namesi | Recommended name: Charged multivesicular body protein 4bAlternative name(s): Chromatin-modifying protein 4b Short name: CHMP4b SNF7 homolog associated with Alix 1 SNF7-2 Short name: hSnf7-2 Vacuolar protein sorting-associated protein 32-2 Short name: Vps32-2 Short name: hVps32-2 |
Gene namesi | Name:CHMP4B Synonyms:C20orf178, SHAX1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000101421.3 |
HGNCi | HGNC:16171, CHMP4B |
MIMi | 610897, gene |
neXtProti | NX_Q9H444 |
Subcellular locationi
Nucleus
- Nucleus envelope 1 Publication
Cytosol
- cytosol 1 Publication
Endosome
- Late endosome membrane 1 Publication1 Publication; Peripheral membrane protein Curated
Other locations
- Midbody 2 Publications
Note: Recruited to the nuclear envelope by CHMP7 during late anaphase (PubMed:26040712). Localizes transiently to the midbody arms immediately before abscission (PubMed:22422861).2 Publications
Cytosol
- cytosol Source: Reactome
Endosome
- endosome Source: UniProtKB
- ESCRT III complex Source: UniProtKB
- late endosome membrane Source: UniProtKB-SubCell
- multivesicular body Source: GO_Central
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- nuclear envelope Source: UniProtKB
- nucleus Source: UniProtKB
Plasma Membrane
- cytoplasmic side of plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- membrane coat Source: UniProtKB
- midbody Source: UniProtKB
- vesicle Source: BHF-UCL
Keywords - Cellular componenti
Cytoplasm, Endosome, Membrane, NucleusPathology & Biotechi
Involvement in diseasei
Cataract 31, multiple types (CTRCT31)1 Publication
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_037579 | 129 | D → V in CTRCT31. 1 PublicationCorresponds to variant dbSNP:rs118203965EnsemblClinVar. | 1 | |
Natural variantiVAR_037580 | 161 | E → K in CTRCT31. 1 PublicationCorresponds to variant dbSNP:rs118203966EnsemblClinVar. | 1 |
Keywords - Diseasei
Cataract, Disease mutationOrganism-specific databases
DisGeNETi | 128866 |
MalaCardsi | CHMP4B |
MIMi | 605387, phenotype |
OpenTargetsi | ENSG00000101421 |
Orphaneti | 98993, Early-onset posterior polar cataract 441447, Early-onset posterior subcapsular cataract |
PharmGKBi | PA25721 |
Miscellaneous databases
Pharosi | Q9H444, Tbio |
Polymorphism and mutation databases
BioMutai | CHMP4B |
DMDMi | 24636296 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000211489 | 2 – 224 | Charged multivesicular body protein 4bAdd BLAST | 223 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources1 Publication | 1 | |
Modified residuei | 6 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 114 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 184 | PhosphoserineCombined sources | 1 | |
Modified residuei | 223 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9H444 |
jPOSTi | Q9H444 |
MassIVEi | Q9H444 |
MaxQBi | Q9H444 |
PaxDbi | Q9H444 |
PeptideAtlasi | Q9H444 |
PRIDEi | Q9H444 |
ProteomicsDBi | 80785 |
PTM databases
iPTMneti | Q9H444 |
MetOSitei | Q9H444 |
PhosphoSitePlusi | Q9H444 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000101421, Expressed in layer of synovial tissue and 212 other tissues |
Genevisiblei | Q9H444, HS |
Organism-specific databases
HPAi | ENSG00000101421, Low tissue specificity |
Interactioni
Subunit structurei
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentially.
Interacts with CHMP6 and CHMP4C.
Interacts with PDCD6IP; the interaction is direct.
Interacts with VPS4A; the interaction is direct.
Interacts with VPS4B; the interaction is direct.
Interacts with CHMP7.
Interacts with CFTR; the interaction requires misfolded CFTR.
Interacts with PTPN23.
14 PublicationsBinary interactionsi
Hide detailsQ9H444
GO - Molecular functioni
- cadherin binding Source: BHF-UCL
- identical protein binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 126170, 136 interactors |
ComplexPortali | CPX-329, ESCRT-III complex |
CORUMi | Q9H444 |
DIPi | DIP-29924N |
IntActi | Q9H444, 125 interactors |
MINTi | Q9H444 |
STRINGi | 9606.ENSP00000217402 |
Miscellaneous databases
RNActi | Q9H444, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9H444 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9H444 |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 23 – 183 | Sequence analysisAdd BLAST | 161 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG1656, Eukaryota |
GeneTreei | ENSGT00940000154663 |
HOGENOMi | CLU_071097_0_0_1 |
InParanoidi | Q9H444 |
OMAi | WSWFGGA |
OrthoDBi | 1490465at2759 |
PhylomeDBi | Q9H444 |
TreeFami | TF314269 |
Family and domain databases
InterProi | View protein in InterPro IPR005024, Snf7_fam |
Pfami | View protein in Pfam PF03357, Snf7, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSVFGKLFGA GGGKAGKGGP TPQEAIQRLR DTEEMLSKKQ EFLEKKIEQE
60 70 80 90 100
LTAAKKHGTK NKRAALQALK RKKRYEKQLA QIDGTLSTIE FQREALENAN
110 120 130 140 150
TNTEVLKNMG YAAKAMKAAH DNMDIDKVDE LMQDIADQQE LAEEISTAIS
160 170 180 190 200
KPVGFGEEFD EDELMAELEE LEQEELDKNL LEISGPETVP LPNVPSIALP
210 220
SKPAKKKEEE DDDMKELENW AGSM
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_037579 | 129 | D → V in CTRCT31. 1 PublicationCorresponds to variant dbSNP:rs118203965EnsemblClinVar. | 1 | |
Natural variantiVAR_037580 | 161 | E → K in CTRCT31. 1 PublicationCorresponds to variant dbSNP:rs118203966EnsemblClinVar. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB100261 mRNA Translation: BAC79375.1 AY329085 mRNA Translation: AAQ91194.1 AL050349 Genomic DNA No translation available. CH471077 Genomic DNA Translation: EAW76293.1 CH471077 Genomic DNA Translation: EAW76294.1 BC033859 mRNA Translation: AAH33859.1 |
CCDSi | CCDS13228.1 |
RefSeqi | NP_789782.1, NM_176812.4 |
Genome annotation databases
Ensembli | ENST00000217402; ENSP00000217402; ENSG00000101421 |
GeneIDi | 128866 |
KEGGi | hsa:128866 |
UCSCi | uc002xaa.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB100261 mRNA Translation: BAC79375.1 AY329085 mRNA Translation: AAQ91194.1 AL050349 Genomic DNA No translation available. CH471077 Genomic DNA Translation: EAW76293.1 CH471077 Genomic DNA Translation: EAW76294.1 BC033859 mRNA Translation: AAH33859.1 |
CCDSi | CCDS13228.1 |
RefSeqi | NP_789782.1, NM_176812.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3C3Q | X-ray | 2.10 | B | 207-224 | [»] | |
3UM3 | X-ray | 3.80 | B | 121-224 | [»] | |
4ABM | X-ray | 1.80 | A/B/C/D | 23-97 | [»] | |
5MK2 | X-ray | 1.70 | C | 205-224 | [»] | |
SMRi | Q9H444 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 126170, 136 interactors |
ComplexPortali | CPX-329, ESCRT-III complex |
CORUMi | Q9H444 |
DIPi | DIP-29924N |
IntActi | Q9H444, 125 interactors |
MINTi | Q9H444 |
STRINGi | 9606.ENSP00000217402 |
PTM databases
iPTMneti | Q9H444 |
MetOSitei | Q9H444 |
PhosphoSitePlusi | Q9H444 |
Polymorphism and mutation databases
BioMutai | CHMP4B |
DMDMi | 24636296 |
Proteomic databases
EPDi | Q9H444 |
jPOSTi | Q9H444 |
MassIVEi | Q9H444 |
MaxQBi | Q9H444 |
PaxDbi | Q9H444 |
PeptideAtlasi | Q9H444 |
PRIDEi | Q9H444 |
ProteomicsDBi | 80785 |
Protocols and materials databases
Antibodypediai | 25737, 166 antibodies |
Genome annotation databases
Ensembli | ENST00000217402; ENSP00000217402; ENSG00000101421 |
GeneIDi | 128866 |
KEGGi | hsa:128866 |
UCSCi | uc002xaa.4, human |
Organism-specific databases
CTDi | 128866 |
DisGeNETi | 128866 |
EuPathDBi | HostDB:ENSG00000101421.3 |
GeneCardsi | CHMP4B |
HGNCi | HGNC:16171, CHMP4B |
HPAi | ENSG00000101421, Low tissue specificity |
MalaCardsi | CHMP4B |
MIMi | 605387, phenotype 610897, gene |
neXtProti | NX_Q9H444 |
OpenTargetsi | ENSG00000101421 |
Orphaneti | 98993, Early-onset posterior polar cataract 441447, Early-onset posterior subcapsular cataract |
PharmGKBi | PA25721 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1656, Eukaryota |
GeneTreei | ENSGT00940000154663 |
HOGENOMi | CLU_071097_0_0_1 |
InParanoidi | Q9H444 |
OMAi | WSWFGGA |
OrthoDBi | 1490465at2759 |
PhylomeDBi | Q9H444 |
TreeFami | TF314269 |
Enzyme and pathway databases
PathwayCommonsi | Q9H444 |
Reactomei | R-HSA-162588, Budding and maturation of HIV virion R-HSA-1632852, Macroautophagy R-HSA-917729, Endosomal Sorting Complex Required For Transport (ESCRT) R-HSA-9610379, HCMV Late Events R-HSA-9615710, Late endosomal microautophagy R-HSA-9668328, Sealing of the nuclear envelope (NE) by ESCRT-III R-HSA-9679504, Translation of Replicase and Assembly of the Replication Transcription Complex |
Miscellaneous databases
BioGRID-ORCSi | 128866, 575 hits in 852 CRISPR screens |
ChiTaRSi | CHMP4B, human |
EvolutionaryTracei | Q9H444 |
GeneWikii | CHMP4B |
GenomeRNAii | 128866 |
Pharosi | Q9H444, Tbio |
PROi | PR:Q9H444 |
RNActi | Q9H444, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000101421, Expressed in layer of synovial tissue and 212 other tissues |
Genevisiblei | Q9H444, HS |
Family and domain databases
InterProi | View protein in InterPro IPR005024, Snf7_fam |
Pfami | View protein in Pfam PF03357, Snf7, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CHM4B_HUMAN | |
Accessioni | Q9H444Primary (citable) accession number: Q9H444 Secondary accession number(s): E1P5N4, Q53ZD6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 2002 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 20
Human chromosome 20: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations