Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Charged multivesicular body protein 4b

Gene

CHMP4B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released (PubMed:12860994, PubMed:18209100). The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (PubMed:21310966). Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase (PubMed:26040712). Plays a role in the endosomal sorting pathway. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4B filaments can promote or stabilize negative curvature and outward budding. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).5 Publications
(Microbial infection) The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the budding of enveloped viruses (HIV-1 and other lentiviruses). Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release.4 Publications

Miscellaneous

Its overexpression strongly inhibits HIV-1 release.

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • identical protein binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-162588 Budding and maturation of HIV virion
R-HSA-1632852 Macroautophagy
R-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Names & Taxonomyi

Protein namesi
Recommended name:
Charged multivesicular body protein 4b
Alternative name(s):
Chromatin-modifying protein 4b
Short name:
CHMP4b
SNF7 homolog associated with Alix 1
SNF7-2
Short name:
hSnf7-2
Vacuolar protein sorting-associated protein 32-2
Short name:
Vps32-2
Short name:
hVps32-2
Gene namesi
Name:CHMP4B
Synonyms:C20orf178, SHAX1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000101421.3
HGNCiHGNC:16171 CHMP4B
MIMi610897 gene
neXtProtiNX_Q9H444

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane, Nucleus

Pathology & Biotechi

Involvement in diseasei

Cataract 31, multiple types (CTRCT31)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. CTRCT31 includes posterior polar, progressive posterior subcapsular, nuclear, and anterior subcapsular cataracts.
See also OMIM:605387
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_037579129D → V in CTRCT31. 1 PublicationCorresponds to variant dbSNP:rs118203965EnsemblClinVar.1
Natural variantiVAR_037580161E → K in CTRCT31. 1 PublicationCorresponds to variant dbSNP:rs118203966EnsemblClinVar.1

Keywords - Diseasei

Cataract, Disease mutation

Organism-specific databases

DisGeNETi128866
MalaCardsiCHMP4B
MIMi605387 phenotype
OpenTargetsiENSG00000101421
Orphaneti98993 Early-onset posterior polar cataract
441447 Early-onset posterior subcapsular cataract
PharmGKBiPA25721

Polymorphism and mutation databases

BioMutaiCHMP4B
DMDMi24636296

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00002114892 – 224Charged multivesicular body protein 4bAdd BLAST223

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1 Publication1
Modified residuei6N6-acetyllysineCombined sources1
Modified residuei114N6-acetyllysineCombined sources1
Modified residuei184PhosphoserineCombined sources1
Modified residuei223PhosphoserineCombined sources1

Post-translational modificationi

ISGylated. Isgylation weakens its interaction with VPS4A.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9H444
MaxQBiQ9H444
PaxDbiQ9H444
PeptideAtlasiQ9H444
PRIDEiQ9H444
ProteomicsDBi80785

PTM databases

iPTMnetiQ9H444
PhosphoSitePlusiQ9H444

Expressioni

Tissue specificityi

Widely expressed. Expressed at higher level in heart and skeletal muscle. Also expressed in brain, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes.1 Publication

Gene expression databases

BgeeiENSG00000101421 Expressed in 197 organ(s), highest expression level in islet of Langerhans
CleanExiHS_CHMP4B
GenevisibleiQ9H444 HS

Organism-specific databases

HPAiHPA041401
HPA051751

Interactioni

Subunit structurei

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentially. Interacts with CHMP6 and CHMP4C. Interacts with PDCD6IP; the interaction is direct. Interacts with VPS4A; the interaction is direct. Interacts with VPS4B; the interaction is direct. Interacts with CHMP7. Interacts with CFTR; the interaction requires misfolded CFTR. Interacts with PTPN23.14 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi126170, 124 interactors
ComplexPortaliCPX-329 ESCRT-III complex
CORUMiQ9H444
DIPiDIP-29924N
IntActiQ9H444, 119 interactors
MINTiQ9H444
STRINGi9606.ENSP00000217402

Structurei

Secondary structure

1224
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9H444
SMRiQ9H444
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9H444

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili23 – 183Sequence analysisAdd BLAST161

Domaini

The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.

Sequence similaritiesi

Belongs to the SNF7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1656 Eukaryota
ENOG410YE9I LUCA
GeneTreeiENSGT00390000005006
HOGENOMiHOG000209960
HOVERGENiHBG050928
InParanoidiQ9H444
KOiK12194
OMAiMQVNTLE
OrthoDBiEOG091G0V7X
PhylomeDBiQ9H444
TreeFamiTF314269

Family and domain databases

InterProiView protein in InterPro
IPR005024 Snf7_fam
PfamiView protein in Pfam
PF03357 Snf7, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9H444-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSVFGKLFGA GGGKAGKGGP TPQEAIQRLR DTEEMLSKKQ EFLEKKIEQE
60 70 80 90 100
LTAAKKHGTK NKRAALQALK RKKRYEKQLA QIDGTLSTIE FQREALENAN
110 120 130 140 150
TNTEVLKNMG YAAKAMKAAH DNMDIDKVDE LMQDIADQQE LAEEISTAIS
160 170 180 190 200
KPVGFGEEFD EDELMAELEE LEQEELDKNL LEISGPETVP LPNVPSIALP
210 220
SKPAKKKEEE DDDMKELENW AGSM
Length:224
Mass (Da):24,950
Last modified:March 1, 2001 - v1
Checksum:iDB1D79DD3803CB2F
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_037579129D → V in CTRCT31. 1 PublicationCorresponds to variant dbSNP:rs118203965EnsemblClinVar.1
Natural variantiVAR_037580161E → K in CTRCT31. 1 PublicationCorresponds to variant dbSNP:rs118203966EnsemblClinVar.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB100261 mRNA Translation: BAC79375.1
AY329085 mRNA Translation: AAQ91194.1
AL050349 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW76293.1
CH471077 Genomic DNA Translation: EAW76294.1
BC033859 mRNA Translation: AAH33859.1
CCDSiCCDS13228.1
RefSeqiNP_789782.1, NM_176812.4
UniGeneiHs.472471

Genome annotation databases

EnsembliENST00000217402; ENSP00000217402; ENSG00000101421
GeneIDi128866
KEGGihsa:128866
UCSCiuc002xaa.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB100261 mRNA Translation: BAC79375.1
AY329085 mRNA Translation: AAQ91194.1
AL050349 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW76293.1
CH471077 Genomic DNA Translation: EAW76294.1
BC033859 mRNA Translation: AAH33859.1
CCDSiCCDS13228.1
RefSeqiNP_789782.1, NM_176812.4
UniGeneiHs.472471

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3C3QX-ray2.10B207-224[»]
3UM3X-ray3.80B121-224[»]
4ABMX-ray1.80A/B/C/D23-97[»]
5MK2X-ray1.70C205-224[»]
ProteinModelPortaliQ9H444
SMRiQ9H444
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126170, 124 interactors
ComplexPortaliCPX-329 ESCRT-III complex
CORUMiQ9H444
DIPiDIP-29924N
IntActiQ9H444, 119 interactors
MINTiQ9H444
STRINGi9606.ENSP00000217402

PTM databases

iPTMnetiQ9H444
PhosphoSitePlusiQ9H444

Polymorphism and mutation databases

BioMutaiCHMP4B
DMDMi24636296

Proteomic databases

EPDiQ9H444
MaxQBiQ9H444
PaxDbiQ9H444
PeptideAtlasiQ9H444
PRIDEiQ9H444
ProteomicsDBi80785

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000217402; ENSP00000217402; ENSG00000101421
GeneIDi128866
KEGGihsa:128866
UCSCiuc002xaa.4 human

Organism-specific databases

CTDi128866
DisGeNETi128866
EuPathDBiHostDB:ENSG00000101421.3
GeneCardsiCHMP4B
HGNCiHGNC:16171 CHMP4B
HPAiHPA041401
HPA051751
MalaCardsiCHMP4B
MIMi605387 phenotype
610897 gene
neXtProtiNX_Q9H444
OpenTargetsiENSG00000101421
Orphaneti98993 Early-onset posterior polar cataract
441447 Early-onset posterior subcapsular cataract
PharmGKBiPA25721
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1656 Eukaryota
ENOG410YE9I LUCA
GeneTreeiENSGT00390000005006
HOGENOMiHOG000209960
HOVERGENiHBG050928
InParanoidiQ9H444
KOiK12194
OMAiMQVNTLE
OrthoDBiEOG091G0V7X
PhylomeDBiQ9H444
TreeFamiTF314269

Enzyme and pathway databases

ReactomeiR-HSA-162588 Budding and maturation of HIV virion
R-HSA-1632852 Macroautophagy
R-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Miscellaneous databases

ChiTaRSiCHMP4B human
EvolutionaryTraceiQ9H444
GeneWikiiCHMP4B
GenomeRNAii128866
PROiPR:Q9H444
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101421 Expressed in 197 organ(s), highest expression level in islet of Langerhans
CleanExiHS_CHMP4B
GenevisibleiQ9H444 HS

Family and domain databases

InterProiView protein in InterPro
IPR005024 Snf7_fam
PfamiView protein in Pfam
PF03357 Snf7, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCHM4B_HUMAN
AccessioniPrimary (citable) accession number: Q9H444
Secondary accession number(s): E1P5N4, Q53ZD6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: March 1, 2001
Last modified: November 7, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again