UniProtKB - Q9H410 (DSN1_HUMAN)
Protein
Kinetochore-associated protein DSN1 homolog
Gene
DSN1
Organism
Homo sapiens (Human)
Status
Functioni
Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.2 Publications
GO - Biological processi
- cell division Source: UniProtKB-KW
- mitotic sister chromatid segregation Source: GO_Central
- mitotic spindle organization Source: Reactome
- neutrophil degranulation Source: Reactome
Keywordsi
Biological process | Cell cycle, Cell division, Chromosome partition, Mitosis |
Enzyme and pathway databases
PathwayCommonsi | Q9H410 |
Reactomei | R-HSA-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-2467813, Separation of Sister Chromatids R-HSA-2500257, Resolution of Sister Chromatid Cohesion R-HSA-5663220, RHO GTPases Activate Formins R-HSA-6798695, Neutrophil degranulation R-HSA-68877, Mitotic Prometaphase R-HSA-9648025, EML4 and NUDC in mitotic spindle formation |
SIGNORi | Q9H410 |
Names & Taxonomyi
Protein namesi | Recommended name: Kinetochore-associated protein DSN1 homolog |
Gene namesi | Name:DSN1 Synonyms:C20orf172, MIS13 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16165, DSN1 |
MIMi | 609175, gene |
neXtProti | NX_Q9H410 |
VEuPathDBi | HostDB:ENSG00000149636.15 |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Other locations
- kinetochore 2 Publications
Note: Associated with the kinetochore.2 Publications
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- extracellular region Source: Reactome
Lysosome
- azurophil granule lumen Source: Reactome
Nucleus
- condensed nuclear chromosome inner kinetochore Source: GO_Central
- fibrillar center Source: HPA
- nuclear body Source: HPA
- nuclear MIS12/MIND complex Source: GO_Central
- nucleolus Source: HPA
- nucleoplasm Source: HPA
Other locations
- condensed chromosome kinetochore Source: WormBase
- kinetochore Source: HPA
- MIS12/MIND type complex Source: UniProtKB
Keywords - Cellular componenti
Centromere, Chromosome, Kinetochore, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 79980 |
OpenTargetsi | ENSG00000149636 |
PharmGKBi | PA162384106 |
Miscellaneous databases
Pharosi | Q9H410, Tbio |
Genetic variation databases
BioMutai | DSN1 |
DMDMi | 28201793 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000079481 | 1 – 356 | Kinetochore-associated protein DSN1 homologAdd BLAST | 356 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 28 | PhosphoserineCombined sources | 1 | |
Modified residuei | 30 | PhosphoserineCombined sources | 1 | |
Modified residuei | 58 | PhosphoserineCombined sources | 1 | |
Modified residuei | 77 | PhosphoserineCombined sources | 1 | |
Modified residuei | 81 | PhosphoserineCombined sources | 1 | |
Modified residuei | 109 | PhosphoserineCombined sources | 1 | |
Modified residuei | 125 | PhosphoserineCombined sources | 1 | |
Cross-linki | 253 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 331 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9H410 |
jPOSTi | Q9H410 |
MassIVEi | Q9H410 |
MaxQBi | Q9H410 |
PaxDbi | Q9H410 |
PeptideAtlasi | Q9H410 |
PRIDEi | Q9H410 |
ProteomicsDBi | 5379 80777 [Q9H410-1] 80778 [Q9H410-2] 80779 [Q9H410-3] |
PTM databases
iPTMneti | Q9H410 |
MetOSitei | Q9H410 |
PhosphoSitePlusi | Q9H410 |
Expressioni
Gene expression databases
Bgeei | ENSG00000149636, Expressed in ventricular zone and 184 other tissues |
ExpressionAtlasi | Q9H410, baseline and differential |
Genevisiblei | Q9H410, HS |
Organism-specific databases
HPAi | ENSG00000149636, Low tissue specificity |
Interactioni
Subunit structurei
Component of the MIS12 complex composed of MIS12, DSN1, NSL1 and PMF1.
Also interacts with KNL1, CBX3 and CBX5.
Interacts with KNSTRN.
3 PublicationsBinary interactionsi
Hide detailsQ9H410
Protein-protein interaction databases
BioGRIDi | 123045, 63 interactors |
ComplexPortali | CPX-5643, Kinetochore MIS12 complex |
CORUMi | Q9H410 |
IntActi | Q9H410, 63 interactors |
MINTi | Q9H410 |
STRINGi | 9606.ENSP00000389810 |
Miscellaneous databases
RNActi | Q9H410, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9H410 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Phylogenomic databases
eggNOGi | ENOG502S0JV, Eukaryota |
GeneTreei | ENSGT00390000011347 |
HOGENOMi | CLU_042801_0_0_1 |
InParanoidi | Q9H410 |
OMAi | FDYMELV |
OrthoDBi | 922920at2759 |
PhylomeDBi | Q9H410 |
TreeFami | TF335504 |
Family and domain databases
InterProi | View protein in InterPro IPR013218, Dsn1/Mis13 |
PANTHERi | PTHR14778, PTHR14778, 1 hit |
Pfami | View protein in Pfam PF08202, MIS13, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9H410-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTSVTRSEII DEKGPVMSKT HDHQLESSLS PVEVFAKTSA SLEMNQGVSE
60 70 80 90 100
ERIHLGSSPK KGGNCDLSHQ ERLQSKSLHL SPQEQSASYQ DRRQSWRRAS
110 120 130 140 150
MKETNRRKSL HPIHQGITEL SRSISVDLAE SKRLGCLLLS SFQFSIQKLE
160 170 180 190 200
PFLRDTKGFS LESFRAKASS LSEELKHFAD GLETDGTLQK CFEDSNGKAS
210 220 230 240 250
DFSLEASVAE MKEYITKFSL ERQTWDQLLL HYQQEAKEIL SRGSTEAKIT
260 270 280 290 300
EVKVEPMTYL GSSQNEVLNT KPDYQKILQN QSKVFDCMEL VMDELQGSVK
310 320 330 340 350
QLQAFMDEST QCFQKVSVQL GKRSMQQLDP SPARKLLKLQ LQNPPAIHGS
GSGSCQ
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5JW53 | Q5JW53_HUMAN | Kinetochore-associated protein DSN1... | DSN1 | 241 | Annotation score: | ||
Q5JW54 | Q5JW54_HUMAN | Kinetochore-associated protein DSN1... | DSN1 | 284 | Annotation score: | ||
Q5JW57 | Q5JW57_HUMAN | Kinetochore-associated protein DSN1... | DSN1 | 141 | Annotation score: |
Sequence cautioni
The sequence BAB14564 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 142 | F → S in BAC04024 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_003826 | 1 – 84 | MTSVT…LSPQE → MIINWNQVSVLWKCLLK in isoform 2. 1 PublicationAdd BLAST | 84 | |
Alternative sequenceiVSP_044281 | 1 – 16 | Missing in isoform 4. 1 PublicationAdd BLAST | 16 | |
Alternative sequenceiVSP_043204 | 12 – 118 | Missing in isoform 3. 1 PublicationAdd BLAST | 107 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK093031 mRNA Translation: BAC04024.1 AK023408 mRNA Translation: BAB14564.1 Frameshift. AK301671 mRNA Translation: BAG63144.1 AK301840 mRNA Translation: BAG63283.1 AL132768 Genomic DNA No translation available. CH471077 Genomic DNA Translation: EAW76101.1 CH471077 Genomic DNA Translation: EAW76102.1 CH471077 Genomic DNA Translation: EAW76104.1 CH471077 Genomic DNA Translation: EAW76106.1 BC058899 mRNA Translation: AAH58899.1 |
CCDSi | CCDS13286.1 [Q9H410-1] CCDS46596.1 [Q9H410-3] CCDS46597.1 [Q9H410-4] |
RefSeqi | NP_001138787.1, NM_001145315.1 [Q9H410-1] NP_001138788.1, NM_001145316.1 [Q9H410-1] NP_001138789.1, NM_001145317.1 [Q9H410-3] NP_001138790.1, NM_001145318.1 [Q9H410-4] NP_079194.3, NM_024918.3 [Q9H410-1] XP_006723939.1, XM_006723876.2 [Q9H410-3] |
Genome annotation databases
Ensembli | ENST00000373734; ENSP00000362839; ENSG00000149636 [Q9H410-3] ENST00000373750; ENSP00000362855; ENSG00000149636 [Q9H410-1] ENST00000426836; ENSP00000389810; ENSG00000149636 [Q9H410-1] ENST00000448110; ENSP00000404463; ENSG00000149636 [Q9H410-4] |
GeneIDi | 79980 |
KEGGi | hsa:79980 |
UCSCi | uc002xga.4, human [Q9H410-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK093031 mRNA Translation: BAC04024.1 AK023408 mRNA Translation: BAB14564.1 Frameshift. AK301671 mRNA Translation: BAG63144.1 AK301840 mRNA Translation: BAG63283.1 AL132768 Genomic DNA No translation available. CH471077 Genomic DNA Translation: EAW76101.1 CH471077 Genomic DNA Translation: EAW76102.1 CH471077 Genomic DNA Translation: EAW76104.1 CH471077 Genomic DNA Translation: EAW76106.1 BC058899 mRNA Translation: AAH58899.1 |
CCDSi | CCDS13286.1 [Q9H410-1] CCDS46596.1 [Q9H410-3] CCDS46597.1 [Q9H410-4] |
RefSeqi | NP_001138787.1, NM_001145315.1 [Q9H410-1] NP_001138788.1, NM_001145316.1 [Q9H410-1] NP_001138789.1, NM_001145317.1 [Q9H410-3] NP_001138790.1, NM_001145318.1 [Q9H410-4] NP_079194.3, NM_024918.3 [Q9H410-1] XP_006723939.1, XM_006723876.2 [Q9H410-3] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5LSI | X-ray | 2.00 | D | 68-200 | [»] | |
5LSJ | X-ray | 3.25 | D/F | 186-356 | [»] | |
5LSK | X-ray | 3.50 | D | 68-356 | [»] | |
SMRi | Q9H410 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 123045, 63 interactors |
ComplexPortali | CPX-5643, Kinetochore MIS12 complex |
CORUMi | Q9H410 |
IntActi | Q9H410, 63 interactors |
MINTi | Q9H410 |
STRINGi | 9606.ENSP00000389810 |
PTM databases
iPTMneti | Q9H410 |
MetOSitei | Q9H410 |
PhosphoSitePlusi | Q9H410 |
Genetic variation databases
BioMutai | DSN1 |
DMDMi | 28201793 |
Proteomic databases
EPDi | Q9H410 |
jPOSTi | Q9H410 |
MassIVEi | Q9H410 |
MaxQBi | Q9H410 |
PaxDbi | Q9H410 |
PeptideAtlasi | Q9H410 |
PRIDEi | Q9H410 |
ProteomicsDBi | 5379 80777 [Q9H410-1] 80778 [Q9H410-2] 80779 [Q9H410-3] |
Protocols and materials databases
Antibodypediai | 1232, 181 antibodies |
DNASUi | 79980 |
Genome annotation databases
Ensembli | ENST00000373734; ENSP00000362839; ENSG00000149636 [Q9H410-3] ENST00000373750; ENSP00000362855; ENSG00000149636 [Q9H410-1] ENST00000426836; ENSP00000389810; ENSG00000149636 [Q9H410-1] ENST00000448110; ENSP00000404463; ENSG00000149636 [Q9H410-4] |
GeneIDi | 79980 |
KEGGi | hsa:79980 |
UCSCi | uc002xga.4, human [Q9H410-1] |
Organism-specific databases
CTDi | 79980 |
DisGeNETi | 79980 |
GeneCardsi | DSN1 |
HGNCi | HGNC:16165, DSN1 |
HPAi | ENSG00000149636, Low tissue specificity |
MIMi | 609175, gene |
neXtProti | NX_Q9H410 |
OpenTargetsi | ENSG00000149636 |
PharmGKBi | PA162384106 |
VEuPathDBi | HostDB:ENSG00000149636.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502S0JV, Eukaryota |
GeneTreei | ENSGT00390000011347 |
HOGENOMi | CLU_042801_0_0_1 |
InParanoidi | Q9H410 |
OMAi | FDYMELV |
OrthoDBi | 922920at2759 |
PhylomeDBi | Q9H410 |
TreeFami | TF335504 |
Enzyme and pathway databases
PathwayCommonsi | Q9H410 |
Reactomei | R-HSA-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-2467813, Separation of Sister Chromatids R-HSA-2500257, Resolution of Sister Chromatid Cohesion R-HSA-5663220, RHO GTPases Activate Formins R-HSA-6798695, Neutrophil degranulation R-HSA-68877, Mitotic Prometaphase R-HSA-9648025, EML4 and NUDC in mitotic spindle formation |
SIGNORi | Q9H410 |
Miscellaneous databases
BioGRID-ORCSi | 79980, 662 hits in 1010 CRISPR screens |
ChiTaRSi | DSN1, human |
GeneWikii | DSN1 |
GenomeRNAii | 79980 |
Pharosi | Q9H410, Tbio |
PROi | PR:Q9H410 |
RNActi | Q9H410, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000149636, Expressed in ventricular zone and 184 other tissues |
ExpressionAtlasi | Q9H410, baseline and differential |
Genevisiblei | Q9H410, HS |
Family and domain databases
InterProi | View protein in InterPro IPR013218, Dsn1/Mis13 |
PANTHERi | PTHR14778, PTHR14778, 1 hit |
Pfami | View protein in Pfam PF08202, MIS13, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DSN1_HUMAN | |
Accessioni | Q9H410Primary (citable) accession number: Q9H410 Secondary accession number(s): B4DWT2 Q9H8P4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2003 |
Last sequence update: | October 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 167 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 20
Human chromosome 20: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references