UniProtKB - Q9H2U1 (DHX36_HUMAN)
ATP-dependent DNA/RNA helicase DHX36
DHX36
Functioni
Multifunctional ATP-dependent helicase that unwinds G-quadruplex (G4) structures (PubMed:16150737, PubMed:18854321, PubMed:20472641, PubMed:21586581).
Plays a role in many biological processes such as genomic integrity, gene expression regulations and as a sensor to initiate antiviral responses (PubMed:14731398, PubMed:18279852, PubMed:21993297, PubMed:22238380, PubMed:25579584).
G4 structures correspond to helical structures containing guanine tetrads (By similarity).
Binds with high affinity to and unwinds G4 structures that are formed in nucleic acids (G4-ADN and G4-RNA) (PubMed:16150737, PubMed:18842585, PubMed:20472641, PubMed:21586581, PubMed:24369427, PubMed:26195789).
Plays a role in genomic integrity (PubMed:22238380).
Converts the G4-RNA structure present in telomerase RNA template component (TREC) into a double-stranded RNA to promote P1 helix formation that acts as a template boundary ensuring accurate reverse transcription (PubMed:20472641, PubMed:21149580, PubMed:21846770, PubMed:22238380, PubMed:24151078, PubMed:25579584).
Plays a role in transcriptional regulation (PubMed:21586581, PubMed:21993297).
Resolves G4-DNA structures in promoters of genes, such as YY1, KIT/c-kit and ALPL and positively regulates their expression (PubMed:21993297).
Plays a role in post-transcriptional regulation (PubMed:27940037).
Unwinds a G4-RNA structure located in the 3'-UTR polyadenylation site of the pre-mRNA TP53 and stimulates TP53 pre-mRNA 3'-end processing in response to ultraviolet (UV)-induced DNA damage (PubMed:27940037).
Binds to the precursor-microRNA-134 (pre-miR-134) terminal loop and regulates its transport into the synapto-dendritic compartment (By similarity).
Involved in the pre-miR-134-dependent inhibition of target gene expression and the control of dendritic spine size (By similarity).
Plays a role in the regulation of cytoplasmic mRNA translation and mRNA stability (PubMed:24369427, PubMed:26489465).
Binds to both G4-RNA structures and alternative non-quadruplex-forming sequence within the 3'-UTR of the PITX1 mRNA regulating negatively PITX1 protein expression (PubMed:24369427).
Binds to both G4-RNA structure in the 5'-UTR and AU-rich elements (AREs) localized in the 3'-UTR of NKX2-5 mRNA to either stimulate protein translation or induce mRNA decay in an ELAVL1-dependent manner, respectively (PubMed:26489465).
Binds also to ARE sequences present in several mRNAs mediating exosome-mediated 3'-5' mRNA degradation (PubMed:14731398, PubMed:18279852).
Involved in cytoplasmic urokinase-type plasminogen activator (uPA) mRNA decay (PubMed:14731398).
Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1 (By similarity).
Required for early embryonic development and hematopoiesis. Involved in the regulation of cardioblast differentiation and proliferation during heart development. Involved in spermatogonia differentiation. May play a role in ossification (By similarity).
By similarity17 PublicationsCatalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 335 | MagnesiumBy similarity | 1 | |
Metal bindingi | 337 | MagnesiumBy similarity | 1 | |
Binding sitei | 557 | ATP; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 233 – 238 | ATPBy similarity | 6 | |
Nucleotide bindingi | 602 – 605 | ATPBy similarity | 4 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- ATP-dependent activity, acting on DNA Source: UniProtKB
- ATP hydrolysis activity Source: RHEA
- DNA helicase activity Source: UniProtKB
- double-stranded RNA binding Source: MGI
- G-quadruplex DNA binding Source: UniProtKB
- G-quadruplex RNA binding Source: UniProtKB
- histone deacetylase binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- mRNA 3'-UTR AU-rich region binding Source: UniProtKB
- mRNA 3'-UTR binding Source: UniProtKB
- mRNA 5'-UTR binding Source: UniProtKB
- pre-miRNA binding Source: UniProtKB
- RNA binding Source: UniProtKB
- RNA helicase activity Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: UniProtKB
- single-stranded DNA binding Source: UniProtKB
- telomerase RNA binding Source: UniProtKB
- transcription cis-regulatory region binding Source: UniProtKB
GO - Biological processi
- 3'-UTR-mediated mRNA destabilization Source: UniProtKB
- cellular response to arsenite ion Source: UniProtKB
- cellular response to heat Source: UniProtKB
- cellular response to UV Source: UniProtKB
- defense response to virus Source: UniProtKB-KW
- G-quadruplex DNA unwinding Source: UniProtKB
- innate immune response Source: UniProtKB-KW
- negative regulation of translation Source: UniProtKB
- ossification Source: UniProtKB
- positive regulation of cardioblast differentiation Source: UniProtKB
- positive regulation of cytoplasmic translation Source: UniProtKB
- positive regulation of dendritic spine morphogenesis Source: UniProtKB
- positive regulation of gene expression Source: UniProtKB
- positive regulation of hematopoietic progenitor cell differentiation Source: UniProtKB
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- positive regulation of interferon-alpha production Source: UniProtKB
- positive regulation of intracellular mRNA localization Source: UniProtKB
- positive regulation of mRNA 3'-end processing Source: UniProtKB
- positive regulation of myeloid dendritic cell cytokine production Source: Ensembl
- positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: UniProtKB
- positive regulation of telomere maintenance Source: UniProtKB
- positive regulation of telomere maintenance via telomere lengthening Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- positive regulation of transcription initiation from RNA polymerase II promoter Source: UniProtKB
- regulation of embryonic development Source: UniProtKB
- regulation of mRNA stability Source: UniProtKB
- regulation of transcription by RNA polymerase III Source: UniProtKB
- regulation of transcription from RNA polymerase II promoter involved in spermatogenesis Source: UniProtKB
- response to exogenous dsRNA Source: Ensembl
- RNA secondary structure unwinding Source: UniProtKB
- spermatogenesis Source: UniProtKB
- telomerase RNA stabilization Source: BHF-UCL
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding, Helicase, Hydrolase, Repressor, RNA-binding |
Biological process | Antiviral defense, Differentiation, Immunity, Innate immunity, Transcription, Transcription regulation, Translation regulation, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9H2U1 |
Reactomei | R-HSA-3134963, DEx/H-box helicases activate type I IFN and inflammatory cytokines production |
SignaLinki | Q9H2U1 |
SIGNORi | Q9H2U1 |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent DNA/RNA helicase DHX36Curated (EC:3.6.4.123 Publications, EC:3.6.4.133 Publications)Alternative name(s): DEAD/H box polypeptide 361 Publication DEAH-box protein 36Curated G4-resolvase-11 Publication Short name: G4R11 Publication MLE-like protein 11 Publication RNA helicase associated with AU-rich element protein1 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:14410, DHX36 |
MIMi | 612767, gene |
neXtProti | NX_Q9H2U1 |
VEuPathDBi | HostDB:ENSG00000174953 |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Cytoplasm and Cytosol
Nucleus
- Nucleus 2 Publications
- Nucleus speckle 1 Publication
Other locations
- Stress granule 1 Publication
- telomere 1 Publication
- Perikaryon By similarity
- dendrite By similarity
- axon By similarity
Note: Predominantly localized in the nucleus (PubMed:18279852). Colocalizes with SRSF2 in nuclear speckles (PubMed:18279852). Colocalizes with DDX5 in nucleolar caps upon transcription inhibition (PubMed:18279852). Accumulates and colocalized with TIA1 in cytoplasmic stress granules (SGs) in an arsenite-, heat shock- and RNA-binding-dependent manner (PubMed:18854321). Shuttles into and out of SGs in an ATPase-dependent manner (PubMed:18854321). Colocalizes in the cytosol with the multi-helicase-TICAM1 complex that translocates to the mitochondria upon poly(I:C) RNA ligand stimulation (By similarity).By similarity2 Publications
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Mitochondrion
- mitochondrion Source: UniProtKB-SubCell
Nucleus
- nuclear speck Source: UniProtKB
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- axon Source: UniProtKB
- chromosome, telomeric region Source: UniProtKB
- cytoplasm Source: UniProtKB
- cytoplasmic stress granule Source: UniProtKB
- dendrite Source: UniProtKB
- perikaryon Source: UniProtKB
Keywords - Cellular componenti
Cell projection, Chromosome, Cytoplasm, Mitochondrion, Nucleus, TelomerePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 54 | P → G: Reduces G4-RNA binding; when associated with A-57, A-59, A-62 and A-63. 1 Publication | 1 | |
Mutagenesisi | 55 | G → L: Inhibits G4-DNA-binding; when associated with L-59 and L-63. 1 Publication | 1 | |
Mutagenesisi | 57 | L → A: Reduces G4-RNA-binding; when associated with G-54, A-59, A-62 and A-63. 1 Publication | 1 | |
Mutagenesisi | 59 | G → A: Reduces G4-RNA-binding; when associated with G-54, A-57, A-62 and A-63. 1 Publication | 1 | |
Mutagenesisi | 59 | G → L: Inhibits G4-DNA-binding; when associated with L-55 and L-63. 1 Publication | 1 | |
Mutagenesisi | 59 | G → P: Greatly reduces G4-RNA-binding; when associated with P-63. 1 Publication | 1 | |
Mutagenesisi | 62 | I → A: Reduces G4-RNA-binding; when associated with G-54, A-57, A-59 and A-63. 1 Publication | 1 | |
Mutagenesisi | 63 | G → A: Reduces G4-RNA-binding; when associated with G-54, A-57, A-59 and A-62. 1 Publication | 1 | |
Mutagenesisi | 63 | G → L: Inhibits G4-DNA-binding; when associated with L-55 and L-59. 1 Publication | 1 | |
Mutagenesisi | 63 | G → P: Greatly reduces G4-RNA-binding; when associated with P-59. 1 Publication | 1 | |
Mutagenesisi | 65 | W → A: Does not inhibit G4-DNA-binding; when associated with A-66. 1 Publication | 1 | |
Mutagenesisi | 66 | Y → A: Does not inhibit G4-DNA-binding; when associated with A-65. 1 Publication | 1 | |
Mutagenesisi | 335 | E → A: Loss of ATPase activity; results in an increased in G4-DNA- and G4-RNA-binding stabilities, increases localization in cytoplasmic stress granules and loss of mRNA deadenylation and mRNA decay. 4 Publications | 1 |
Organism-specific databases
DisGeNETi | 170506 |
OpenTargetsi | ENSG00000174953 |
PharmGKBi | PA27223 |
Miscellaneous databases
Pharosi | Q9H2U1, Tbio |
Chemistry databases
ChEMBLi | CHEMBL2040704 |
Genetic variation databases
BioMutai | DHX36 |
DMDMi | 313104099 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000247530 | 1 – 1008 | ATP-dependent DNA/RNA helicase DHX36Add BLAST | 1008 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 161 | PhosphoserineCombined sources | 1 | |
Modified residuei | 947 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 963 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9H2U1 |
jPOSTi | Q9H2U1 |
MassIVEi | Q9H2U1 |
MaxQBi | Q9H2U1 |
PaxDbi | Q9H2U1 |
PeptideAtlasi | Q9H2U1 |
PRIDEi | Q9H2U1 |
ProteomicsDBi | 80591 [Q9H2U1-1] 80592 [Q9H2U1-2] 80593 [Q9H2U1-3] |
PTM databases
GlyGeni | Q9H2U1, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9H2U1 |
MetOSitei | Q9H2U1 |
PhosphoSitePlusi | Q9H2U1 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000174953, Expressed in testis and 219 other tissues |
ExpressionAtlasi | Q9H2U1, baseline and differential |
Genevisiblei | Q9H2U1, HS |
Organism-specific databases
HPAi | ENSG00000174953, Low tissue specificity |
Interactioni
Subunit structurei
Found in a multi-helicase-TICAM1 complex at least composed of DHX36, DDX1, DDX21 and TICAM1; this complex exists in resting cells with or without dsRNA poly(I:C) ligand stimulation (By similarity).
Interacts (via C-terminus) with TICAM1 (via TIR domain) (By similarity).
Interacts (via C-terminus) with DDX21; this interaction serves as bridges to TICAM1 (By similarity).
Interacts with TERT; this interaction is dependent on the ability of DHX36 to bind to the G-quadruplex RNA (G4-RNA) structure present in the telomerase RNA template component (TERC) (PubMed:21846770).
Interacts with DKC1; this interaction is dependent on the ability of DHX36 to bind to the G4-RNA structure present in TERC (PubMed:21846770).
Interacts with PARN; this interaction stimulates PARN to enhance uPA mRNA decay (PubMed:14731398).
Interacts with EXOSC3; this interaction occurs in a RNase-insensitive manner (PubMed:14731398).
Interacts with EXOSC10; this interaction occurs in a RNase-insensitive manner (PubMed:14731398).
Interacts with ILF3; this interaction occurs in a RNA-dependent manner (PubMed:14731398).
Interacts with ELAVL1; this interaction occurs in an RNA-dependent manner (PubMed:14731398, PubMed:26489465).
Interacts with DDX5; this interaction occurs in a RNA-dependent manner (PubMed:18279852).
Interacts with DDX17; this interaction occurs in a RNA-dependent manner (PubMed:18279852).
Interacts with HDAC1; this interaction occurs in a RNA-dependent manner (PubMed:18279852).
Interacts with HDAC3; this interaction occurs in a RNA-dependent manner (PubMed:18279852).
Interacts with HDAC4 (By similarity).
Interacts with AGO1 (PubMed:17932509).
Interacts with AGO2 (PubMed:17932509).
Interacts with ERCC6 (PubMed:26030138).
By similarity6 PublicationsBinary interactionsi
Isoform 3 [Q9H2U1-3]
With | #Exp. | IntAct |
---|---|---|
HMOX2 [P30519] | 3 | EBI-25868628,EBI-712096 |
RAN [P62826] | 3 | EBI-25868628,EBI-286642 |
GO - Molecular functioni
- histone deacetylase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 128022, 237 interactors |
IntActi | Q9H2U1, 33 interactors |
MINTi | Q9H2U1 |
STRINGi | 9606.ENSP00000417078 |
Miscellaneous databases
RNActi | Q9H2U1, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9H2U1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 217 – 387 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 171 | |
Domaini | 477 – 647 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 171 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 200 | Necessary for nuclear and nucleolar caps localizations1 PublicationAdd BLAST | 200 | |
Regioni | 1 – 104 | Required for the pre-miR-134 transportBy similarityAdd BLAST | 104 | |
Regioni | 1 – 58 | DisorderedSequence analysisAdd BLAST | 58 | |
Regioni | 1 – 51 | Required for recruitment to cytoplasmic stress granules1 PublicationAdd BLAST | 51 | |
Regioni | 53 – 105 | Required for G4-DNA- and G4-RNA-binding3 PublicationsAdd BLAST | 53 | |
Regioni | 53 – 75 | DSM (DHX36-specific motif)5 PublicationsAdd BLAST | 23 | |
Regioni | 106 – 386 | RecA-like domain 1By similarityAdd BLAST | 281 | |
Regioni | 265 – 317 | Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structureBy similarityAdd BLAST | 53 | |
Regioni | 387 – 628 | RecA-like domain 2By similarityAdd BLAST | 242 | |
Regioni | 498 – 557 | Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structureBy similarityAdd BLAST | 60 | |
Regioni | 629 – 698 | WH domainBy similarityAdd BLAST | 70 | |
Regioni | 638 – 697 | Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structureBy similarityAdd BLAST | 60 | |
Regioni | 841 – 905 | OB-fold-like subdomainsBy similarityAdd BLAST | 65 | |
Regioni | 849 – 860 | Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structureBy similarityAdd BLAST | 12 | |
Regioni | 870 – 900 | Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structureBy similarityAdd BLAST | 31 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 72 – 157 | Sequence analysisAdd BLAST | 86 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 334 – 337 | DEAH boxPROSITE-ProRule annotation | 4 | |
Motifi | 517 – 528 | Nuclear localization signal1 PublicationAdd BLAST | 12 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG0920, Eukaryota |
GeneTreei | ENSGT00940000156903 |
InParanoidi | Q9H2U1 |
OMAi | IEGVMFP |
OrthoDBi | 278674at2759 |
PhylomeDBi | Q9H2U1 |
TreeFami | TF324744 |
Family and domain databases
Gene3Di | 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR002464, DNA/RNA_helicase_DEAH_CS IPR011709, DUF1605 IPR007502, Helicase-assoc_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF04408, HA2, 1 hit PF00271, Helicase_C, 1 hit PF07717, OB_NTP_bind, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00847, HA2, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00690, DEAH_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSYDYHQNWG RDGGPRSSGG GYGGGPAGGH GGNRGSGGGG GGGGGGRGGR
60 70 80 90 100
GRHPGHLKGR EIGMWYAKKQ GQKNKEAERQ ERAVVHMDER REEQIVQLLN
110 120 130 140 150
SVQAKNDKES EAQISWFAPE DHGYGTEVST KNTPCSENKL DIQEKKLINQ
160 170 180 190 200
EKKMFRIRNR SYIDRDSEYL LQENEPDGTL DQKLLEDLQK KKNDLRYIEM
210 220 230 240 250
QHFREKLPSY GMQKELVNLI DNHQVTVISG ETGCGKTTQV TQFILDNYIE
260 270 280 290 300
RGKGSACRIV CTQPRRISAI SVAERVAAER AESCGSGNST GYQIRLQSRL
310 320 330 340 350
PRKQGSILYC TTGIILQWLQ SDPYLSSVSH IVLDEIHERN LQSDVLMTVV
360 370 380 390 400
KDLLNFRSDL KVILMSATLN AEKFSEYFGN CPMIHIPGFT FPVVEYLLED
410 420 430 440 450
VIEKIRYVPE QKEHRSQFKR GFMQGHVNRQ EKEEKEAIYK ERWPDYVREL
460 470 480 490 500
RRRYSASTVD VIEMMEDDKV DLNLIVALIR YIVLEEEDGA ILVFLPGWDN
510 520 530 540 550
ISTLHDLLMS QVMFKSDKFL IIPLHSLMPT VNQTQVFKRT PPGVRKIVIA
560 570 580 590 600
TNIAETSITI DDVVYVIDGG KIKETHFDTQ NNISTMSAEW VSKANAKQRK
610 620 630 640 650
GRAGRVQPGH CYHLYNGLRA SLLDDYQLPE ILRTPLEELC LQIKILRLGG
660 670 680 690 700
IAYFLSRLMD PPSNEAVLLS IRHLMELNAL DKQEELTPLG VHLARLPVEP
710 720 730 740 750
HIGKMILFGA LFCCLDPVLT IAASLSFKDP FVIPLGKEKI ADARRKELAK
760 770 780 790 800
DTRSDHLTVV NAFEGWEEAR RRGFRYEKDY CWEYFLSSNT LQMLHNMKGQ
810 820 830 840 850
FAEHLLGAGF VSSRNPKDPE SNINSDNEKI IKAVICAGLY PKVAKIRLNL
860 870 880 890 900
GKKRKMVKVY TKTDGLVAVH PKSVNVEQTD FHYNWLIYHL KMRTSSIYLY
910 920 930 940 950
DCTEVSPYCL LFFGGDISIQ KDNDQETIAV DEWIVFQSPA RIAHLVKELR
960 970 980 990 1000
KELDILLQEK IESPHPVDWN DTKSRDCAVL SAIIDLIKTQ EKATPRNFPP
RFQDGYYS
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE7EWK3 | E7EWK3_HUMAN | RNA helicase | DHX36 | 797 | Annotation score: | ||
H7C514 | H7C514_HUMAN | ATP-dependent DNA/RNA helicase DHX3... | DHX36 | 119 | Annotation score: | ||
H7C5F5 | H7C5F5_HUMAN | ATP-dependent DNA/RNA helicase DHX3... | DHX36 | 187 | Annotation score: | ||
A0A0G2JQU7 | A0A0G2JQU7_HUMAN | ATP-dependent DNA/RNA helicase DHX3... | DHX36 | 216 | Annotation score: | ||
A0A0G2JRP3 | A0A0G2JRP3_HUMAN | ATP-dependent DNA/RNA helicase DHX3... | DHX36 | 91 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_027140 | 151 | E → K. Corresponds to variant dbSNP:rs1058299Ensembl. | 1 | |
Natural variantiVAR_027141 | 416 | S → C3 PublicationsCorresponds to variant dbSNP:rs9438Ensembl. | 1 | |
Natural variantiVAR_027142 | 583 | I → N1 PublicationCorresponds to variant dbSNP:rs17853513Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020006 | 517 – 530 | Missing in isoform 2. 1 PublicationAdd BLAST | 14 | |
Alternative sequenceiVSP_020007 | 737 – 765 | Missing in isoform 3. 1 PublicationAdd BLAST | 29 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF217190 mRNA Translation: AAG36783.1 AJ577133 mRNA Translation: CAE11802.1 AJ577134 mRNA Translation: CAE11803.1 AK314435 mRNA Translation: BAG37047.1 AC018452 Genomic DNA No translation available. AC134026 Genomic DNA No translation available. CH471052 Genomic DNA Translation: EAW78761.1 BC036035 mRNA Translation: AAH36035.1 AB040921 mRNA Translation: BAA96012.1 |
CCDSi | CCDS3171.1 [Q9H2U1-1] CCDS54657.1 [Q9H2U1-2] |
PIRi | D56236 |
RefSeqi | NP_001107869.1, NM_001114397.1 [Q9H2U1-2] NP_065916.2, NM_020865.2 [Q9H2U1-1] |
Genome annotation databases
Ensembli | ENST00000308361; ENSP00000309296; ENSG00000174953 [Q9H2U1-3] ENST00000329463; ENSP00000330113; ENSG00000174953 [Q9H2U1-2] ENST00000496811; ENSP00000417078; ENSG00000174953 |
GeneIDi | 170506 |
KEGGi | hsa:170506 |
MANE-Selecti | ENST00000496811.6; ENSP00000417078.1; NM_020865.3; NP_065916.2 |
UCSCi | uc003ezy.5, human [Q9H2U1-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF217190 mRNA Translation: AAG36783.1 AJ577133 mRNA Translation: CAE11802.1 AJ577134 mRNA Translation: CAE11803.1 AK314435 mRNA Translation: BAG37047.1 AC018452 Genomic DNA No translation available. AC134026 Genomic DNA No translation available. CH471052 Genomic DNA Translation: EAW78761.1 BC036035 mRNA Translation: AAH36035.1 AB040921 mRNA Translation: BAA96012.1 |
CCDSi | CCDS3171.1 [Q9H2U1-1] CCDS54657.1 [Q9H2U1-2] |
PIRi | D56236 |
RefSeqi | NP_001107869.1, NM_001114397.1 [Q9H2U1-2] NP_065916.2, NM_020865.2 [Q9H2U1-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2N16 | NMR | - | A | 53-70 | [»] | |
2N21 | NMR | - | A | 53-70 | [»] | |
6Q6R | X-ray | 1.50 | E/F/G/H | 53-81 | [»] | |
SMRi | Q9H2U1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 128022, 237 interactors |
IntActi | Q9H2U1, 33 interactors |
MINTi | Q9H2U1 |
STRINGi | 9606.ENSP00000417078 |
Chemistry databases
ChEMBLi | CHEMBL2040704 |
PTM databases
GlyGeni | Q9H2U1, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9H2U1 |
MetOSitei | Q9H2U1 |
PhosphoSitePlusi | Q9H2U1 |
Genetic variation databases
BioMutai | DHX36 |
DMDMi | 313104099 |
Proteomic databases
EPDi | Q9H2U1 |
jPOSTi | Q9H2U1 |
MassIVEi | Q9H2U1 |
MaxQBi | Q9H2U1 |
PaxDbi | Q9H2U1 |
PeptideAtlasi | Q9H2U1 |
PRIDEi | Q9H2U1 |
ProteomicsDBi | 80591 [Q9H2U1-1] 80592 [Q9H2U1-2] 80593 [Q9H2U1-3] |
Protocols and materials databases
Antibodypediai | 33625, 177 antibodies from 27 providers |
DNASUi | 170506 |
Genome annotation databases
Ensembli | ENST00000308361; ENSP00000309296; ENSG00000174953 [Q9H2U1-3] ENST00000329463; ENSP00000330113; ENSG00000174953 [Q9H2U1-2] ENST00000496811; ENSP00000417078; ENSG00000174953 |
GeneIDi | 170506 |
KEGGi | hsa:170506 |
MANE-Selecti | ENST00000496811.6; ENSP00000417078.1; NM_020865.3; NP_065916.2 |
UCSCi | uc003ezy.5, human [Q9H2U1-1] |
Organism-specific databases
CTDi | 170506 |
DisGeNETi | 170506 |
GeneCardsi | DHX36 |
HGNCi | HGNC:14410, DHX36 |
HPAi | ENSG00000174953, Low tissue specificity |
MIMi | 612767, gene |
neXtProti | NX_Q9H2U1 |
OpenTargetsi | ENSG00000174953 |
PharmGKBi | PA27223 |
VEuPathDBi | HostDB:ENSG00000174953 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0920, Eukaryota |
GeneTreei | ENSGT00940000156903 |
InParanoidi | Q9H2U1 |
OMAi | IEGVMFP |
OrthoDBi | 278674at2759 |
PhylomeDBi | Q9H2U1 |
TreeFami | TF324744 |
Enzyme and pathway databases
PathwayCommonsi | Q9H2U1 |
Reactomei | R-HSA-3134963, DEx/H-box helicases activate type I IFN and inflammatory cytokines production |
SignaLinki | Q9H2U1 |
SIGNORi | Q9H2U1 |
Miscellaneous databases
BioGRID-ORCSi | 170506, 618 hits in 1063 CRISPR screens |
ChiTaRSi | DHX36, human |
GeneWikii | DHX36 |
GenomeRNAii | 170506 |
Pharosi | Q9H2U1, Tbio |
PROi | PR:Q9H2U1 |
RNActi | Q9H2U1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000174953, Expressed in testis and 219 other tissues |
ExpressionAtlasi | Q9H2U1, baseline and differential |
Genevisiblei | Q9H2U1, HS |
Family and domain databases
Gene3Di | 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR002464, DNA/RNA_helicase_DEAH_CS IPR011709, DUF1605 IPR007502, Helicase-assoc_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF04408, HA2, 1 hit PF00271, Helicase_C, 1 hit PF07717, OB_NTP_bind, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00847, HA2, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00690, DEAH_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DHX36_HUMAN | |
Accessioni | Q9H2U1Primary (citable) accession number: Q9H2U1 Secondary accession number(s): B2RB00 Q9P240 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 25, 2006 |
Last sequence update: | November 30, 2010 | |
Last modified: | February 23, 2022 | |
This is version 184 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families