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Protein

Zinc finger protein Eos

Gene

IKZF4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems. Essential for the inhibitory function of regulatory T-cells (Treg). Mediates FOXP3-mediated gene silencing in regulatory T-cells (Treg) via recruitment of corepressor CTBP1 (By similarity).By similarity3 Publications

Miscellaneous

'Eos' means 'rising sun' in Greek.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri159 – 181C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri187 – 209C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri215 – 237C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri248 – 271C2H2-type 4PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri530 – 552C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri558 – 582C2H2-type 6PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

  • bHLH transcription factor binding Source: Ensembl
  • DNA-binding transcription factor activity Source: GO_Central
  • DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
  • protein domain specific binding Source: CAFA
  • sequence-specific DNA binding Source: Ensembl
  • transcription regulatory region DNA binding Source: GO_Central
  • zinc ion binding Source: CAFA

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

SIGNORiQ9H2S9

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein Eos
Alternative name(s):
Ikaros family zinc finger protein 4
Gene namesi
Name:IKZF4
Synonyms:KIAA1782, ZNFN1A4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000123411.14
HGNCiHGNC:13179 IKZF4
MIMi606239 gene
neXtProtiNX_Q9H2S9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi425 – 427PED → AAA: No effect on CTBP2 interaction. 1 Publication3

Organism-specific databases

DisGeNETi64375
OpenTargetsiENSG00000123411
PharmGKBiPA162391948

Polymorphism and mutation databases

BioMutaiIKZF4
DMDMi158564025

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002994681 – 585Zinc finger protein EosAdd BLAST585

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki100Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei105PhosphoserineBy similarity1
Modified residuei335N6-acetyllysineBy similarity1
Cross-linki500Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ9H2S9
PaxDbiQ9H2S9
PeptideAtlasiQ9H2S9
PRIDEiQ9H2S9
ProteomicsDBi80588
80589 [Q9H2S9-2]

PTM databases

iPTMnetiQ9H2S9
PhosphoSitePlusiQ9H2S9

Expressioni

Tissue specificityi

Highly expressed in skeletal muscle, low levels of expression in heart, thymus, kidney, liver, and spleen. Expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K-562. Highly expressed in THP-1 and M-07e cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively.1 Publication

Gene expression databases

BgeeiENSG00000123411 Expressed in 185 organ(s), highest expression level in right adrenal gland
CleanExiHS_IKZF4
ExpressionAtlasiQ9H2S9 baseline and differential
GenevisibleiQ9H2S9 HS

Organism-specific databases

HPAiHPA046270
HPA049016

Interactioni

Subunit structurei

Self-associates. Interacts with other family members; IKZF1, IKZF2, IKZF3 and IKZF5. Interacts with CTBP2. Interacts with SPI1, MITF, FOXP3 and CTBP1 (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi122146, 16 interactors
CORUMiQ9H2S9
ELMiQ9H2S9
IntActiQ9H2S9, 10 interactors
STRINGi9606.ENSP00000262032

Structurei

Secondary structure

1585
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00376
ProteinModelPortaliQ9H2S9
SMRiQ9H2S9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni281 – 585Interaction with FOXP3By similarityAdd BLAST305

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi425 – 429CTBP-binding motif PEDLA5

Domaini

The N-terminal zinc fingers are involved in sequence-specific DNA binding and heterotypic associations with other family members.
C-terminal zinc fingers mediate homodimerization.

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri159 – 181C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri187 – 209C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri215 – 237C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri248 – 271C2H2-type 4PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri530 – 552C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri558 – 582C2H2-type 6PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00550000074392
HOGENOMiHOG000049114
HOVERGENiHBG004752
InParanoidiQ9H2S9
KOiK09220
OMAiESNHEER
OrthoDBiEOG091G05E2
PhylomeDBiQ9H2S9
TreeFamiTF331189

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 6 hits
SUPFAMiSSF57667 SSF57667, 3 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 5 hits
PS50157 ZINC_FINGER_C2H2_2, 4 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9H2S9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MHTPPALPRR FQGGGRVRTP GSHRQGKDNL ERDPSGGCVP DFLPQAQDSN
60 70 80 90 100
HFIMESLFCE SSGDSSLEKE FLGAPVGPSV STPNSQHSSP SRSLSANSIK
110 120 130 140 150
VEMYSDEESS RLLGPDERLL EKDDSVIVED SLSEPLGYCD GSGPEPHSPG
160 170 180 190 200
GIRLPNGKLK CDVCGMVCIG PNVLMVHKRS HTGERPFHCN QCGASFTQKG
210 220 230 240 250
NLLRHIKLHS GEKPFKCPFC NYACRRRDAL TGHLRTHSVS SPTVGKPYKC
260 270 280 290 300
NYCGRSYKQQ STLEEHKERC HNYLQSLSTE AQALAGQPGD EIRDLEMVPD
310 320 330 340 350
SMLHSSSERP TFIDRLANSL TKRKRSTPQK FVGEKQMRFS LSDLPYDVNS
360 370 380 390 400
GGYEKDVELV AHHSLEPGFG SSLAFVGAEH LRPLRLPPTN CISELTPVIS
410 420 430 440 450
SVYTQMQPLP GRLELPGSRE AGEGPEDLAD GGPLLYRPRG PLTDPGASPS
460 470 480 490 500
NGCQDSTDTE SNHEDRVAGV VSLPQGPPPQ PPPTIVVGRH SPAYAKEDPK
510 520 530 540 550
PQEGLLRGTP GPSKEVLRVV GESGEPVKAF KCEHCRILFL DHVMFTIHMG
560 570 580
CHGFRDPFEC NICGYHSQDR YEFSSHIVRG EHKVG
Length:585
Mass (Da):64,106
Last modified:September 11, 2007 - v2
Checksum:i42AE169A3E2E14D3
GO
Isoform 2 (identifier: Q9H2S9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: MHTPPALPRR...FIMESLFCES → MDSRYLQLQLYLPSCSLLQG

Note: No experimental confirmation available.
Show »
Length:544
Mass (Da):59,671
Checksum:i68743F2C2449BD84
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8VPL6F8VPL6_HUMAN
Zinc finger protein Eos
IKZF4
540Annotation score:
F8VW19F8VW19_HUMAN
Zinc finger protein Eos
IKZF4
48Annotation score:
A0A0G2JL44A0A0G2JL44_HUMAN
Zinc finger protein Eos
IKZF4
10Annotation score:

Sequence cautioni

The sequence AAG39221 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB47411 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti145E → K in AAG39221 (PubMed:10978333).Curated1
Sequence conflicti310P → A in AAG39221 (PubMed:10978333).Curated1
Sequence conflicti361A → G in AAG39221 (PubMed:10978333).Curated1
Sequence conflicti390N → H in AAG39221 (PubMed:10978333).Curated1
Sequence conflicti524G → S in AAG39221 (PubMed:10978333).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0276881 – 61MHTPP…LFCES → MDSRYLQLQLYLPSCSLLQG in isoform 2. 1 PublicationAdd BLAST61

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB058685 mRNA Translation: BAB47411.1 Different initiation.
BX647761 mRNA No translation available.
AF230809 mRNA Translation: AAG39221.1 Different initiation.
CCDSiCCDS44917.1 [Q9H2S9-1]
RefSeqiNP_071910.3, NM_022465.3 [Q9H2S9-1]
XP_016875294.1, XM_017019805.1 [Q9H2S9-1]
XP_016875295.1, XM_017019806.1 [Q9H2S9-1]
XP_016875296.1, XM_017019807.1 [Q9H2S9-1]
XP_016875297.1, XM_017019808.1 [Q9H2S9-1]
XP_016875298.1, XM_017019809.1 [Q9H2S9-1]
XP_016875299.1, XM_017019810.1 [Q9H2S9-1]
XP_016875300.1, XM_017019811.1 [Q9H2S9-1]
UniGeneiHs.553221

Genome annotation databases

EnsembliENST00000262032; ENSP00000262032; ENSG00000123411 [Q9H2S9-1]
ENST00000431367; ENSP00000412101; ENSG00000123411 [Q9H2S9-1]
ENST00000547167; ENSP00000448419; ENSG00000123411 [Q9H2S9-1]
GeneIDi64375
KEGGihsa:64375
UCSCiuc001sjb.1 human [Q9H2S9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB058685 mRNA Translation: BAB47411.1 Different initiation.
BX647761 mRNA No translation available.
AF230809 mRNA Translation: AAG39221.1 Different initiation.
CCDSiCCDS44917.1 [Q9H2S9-1]
RefSeqiNP_071910.3, NM_022465.3 [Q9H2S9-1]
XP_016875294.1, XM_017019805.1 [Q9H2S9-1]
XP_016875295.1, XM_017019806.1 [Q9H2S9-1]
XP_016875296.1, XM_017019807.1 [Q9H2S9-1]
XP_016875297.1, XM_017019808.1 [Q9H2S9-1]
XP_016875298.1, XM_017019809.1 [Q9H2S9-1]
XP_016875299.1, XM_017019810.1 [Q9H2S9-1]
XP_016875300.1, XM_017019811.1 [Q9H2S9-1]
UniGeneiHs.553221

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MA7NMR-A155-216[»]
DisProtiDP00376
ProteinModelPortaliQ9H2S9
SMRiQ9H2S9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122146, 16 interactors
CORUMiQ9H2S9
ELMiQ9H2S9
IntActiQ9H2S9, 10 interactors
STRINGi9606.ENSP00000262032

PTM databases

iPTMnetiQ9H2S9
PhosphoSitePlusiQ9H2S9

Polymorphism and mutation databases

BioMutaiIKZF4
DMDMi158564025

Proteomic databases

MaxQBiQ9H2S9
PaxDbiQ9H2S9
PeptideAtlasiQ9H2S9
PRIDEiQ9H2S9
ProteomicsDBi80588
80589 [Q9H2S9-2]

Protocols and materials databases

DNASUi64375
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000262032; ENSP00000262032; ENSG00000123411 [Q9H2S9-1]
ENST00000431367; ENSP00000412101; ENSG00000123411 [Q9H2S9-1]
ENST00000547167; ENSP00000448419; ENSG00000123411 [Q9H2S9-1]
GeneIDi64375
KEGGihsa:64375
UCSCiuc001sjb.1 human [Q9H2S9-1]

Organism-specific databases

CTDi64375
DisGeNETi64375
EuPathDBiHostDB:ENSG00000123411.14
GeneCardsiIKZF4
HGNCiHGNC:13179 IKZF4
HPAiHPA046270
HPA049016
MIMi606239 gene
neXtProtiNX_Q9H2S9
OpenTargetsiENSG00000123411
PharmGKBiPA162391948
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00550000074392
HOGENOMiHOG000049114
HOVERGENiHBG004752
InParanoidiQ9H2S9
KOiK09220
OMAiESNHEER
OrthoDBiEOG091G05E2
PhylomeDBiQ9H2S9
TreeFamiTF331189

Enzyme and pathway databases

SIGNORiQ9H2S9

Miscellaneous databases

ChiTaRSiIKZF4 human
GeneWikiiIKZF4
GenomeRNAii64375
PROiPR:Q9H2S9
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000123411 Expressed in 185 organ(s), highest expression level in right adrenal gland
CleanExiHS_IKZF4
ExpressionAtlasiQ9H2S9 baseline and differential
GenevisibleiQ9H2S9 HS

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 6 hits
SUPFAMiSSF57667 SSF57667, 3 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 5 hits
PS50157 ZINC_FINGER_C2H2_2, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiIKZF4_HUMAN
AccessioniPrimary (citable) accession number: Q9H2S9
Secondary accession number(s): Q96JP3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: November 7, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
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