UniProtKB - Q9H2S1 (KCNN2_HUMAN)
Protein
Small conductance calcium-activated potassium channel protein 2
Gene
KCNN2
Organism
Homo sapiens (Human)
Status
Functioni
Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin.
GO - Molecular functioni
- alpha-actinin binding Source: BHF-UCL
- calcium-activated potassium channel activity Source: BHF-UCL
- calmodulin binding Source: GO_Central
- protein domain specific binding Source: UniProtKB
- protein homodimerization activity Source: BHF-UCL
- small conductance calcium-activated potassium channel activity Source: BHF-UCL
GO - Biological processi
- ion transport Source: GO_Central
- membrane repolarization during atrial cardiac muscle cell action potential Source: BHF-UCL
- potassium ion transmembrane transport Source: BHF-UCL
- potassium ion transport Source: UniProtKB
- regulation of potassium ion transmembrane transport Source: BHF-UCL
Keywordsi
Molecular function | Calmodulin-binding, Ion channel |
Biological process | Ion transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q9H2S1 |
Reactomei | R-HSA-1296052, Ca2+ activated K+ channels |
SIGNORi | Q9H2S1 |
Protein family/group databases
TCDBi | 1.A.1.16.1, the voltage-gated ion channel (vic) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Small conductance calcium-activated potassium channel protein 2Short name: SK2 Short name: SKCa 2 Short name: SKCa2 Alternative name(s): KCa2.2 |
Gene namesi | Name:KCNN2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000080709.14 |
HGNCi | HGNC:6291, KCNN2 |
MIMi | 605879, gene |
neXtProti | NX_Q9H2S1 |
Subcellular locationi
Other locations
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cell surface Source: UniProtKB
- dendritic spine Source: GO_Central
- integral component of membrane Source: UniProtKB
- neuron projection Source: GO_Central
- neuronal cell body Source: GO_Central
- Z disc Source: BHF-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 138 – 158 | Helical; Name=Segment S1Sequence analysisAdd BLAST | 21 | |
Transmembranei | 168 – 188 | Helical; Name=Segment S2Sequence analysisAdd BLAST | 21 | |
Transmembranei | 214 – 234 | Helical; Name=Segment S3Sequence analysisAdd BLAST | 21 | |
Transmembranei | 256 – 276 | Helical; Name=Segment S4Sequence analysisAdd BLAST | 21 | |
Transmembranei | 305 – 325 | Helical; Name=Segment S5Sequence analysisAdd BLAST | 21 | |
Intramembranei | 345 – 365 | Pore-forming; Name=Segment H5Sequence analysisAdd BLAST | 21 | |
Transmembranei | 374 – 394 | Helical; Name=Segment S6Sequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 3781 |
OpenTargetsi | ENSG00000080709 |
PharmGKBi | PA30071 |
Miscellaneous databases
Pharosi | Q9H2S1, Tchem |
Chemistry databases
ChEMBLi | CHEMBL4469 |
DrugBanki | DB01110, Miconazole DB00721, Procaine DB09089, Trimebutine |
DrugCentrali | Q9H2S1 |
GuidetoPHARMACOLOGYi | 382 |
Polymorphism and mutation databases
BioMutai | KCNN2 |
DMDMi | 209572638 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000155010 | 1 – 579 | Small conductance calcium-activated potassium channel protein 2Add BLAST | 579 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 160 | PhosphotyrosineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9H2S1 |
jPOSTi | Q9H2S1 |
MassIVEi | Q9H2S1 |
MaxQBi | Q9H2S1 |
PaxDbi | Q9H2S1 |
PeptideAtlasi | Q9H2S1 |
PRIDEi | Q9H2S1 |
ProteomicsDBi | 67208 80583 [Q9H2S1-1] |
PTM databases
iPTMneti | Q9H2S1 |
PhosphoSitePlusi | Q9H2S1 |
Expressioni
Tissue specificityi
Expressed in atrial myocytes (at protein level). Widely expressed.1 Publication
Gene expression databases
Bgeei | ENSG00000080709, Expressed in right adrenal gland and 171 other tissues |
ExpressionAtlasi | Q9H2S1, baseline and differential |
Genevisiblei | Q9H2S1, HS |
Organism-specific databases
HPAi | ENSG00000080709, Tissue enhanced (adrenal gland, brain, liver) |
Interactioni
Subunit structurei
Heterooligomer. The complex is composed of 4 channel subunits each of which binds to a calmodulin subunit which regulates the channel activity through calcium-binding (By similarity).
By similarityBinary interactionsi
Q9H2S1
With | #Exp. | IntAct |
---|---|---|
ACTN2 [P35609] | 3 | EBI-6658875,EBI-77797 |
GO - Molecular functioni
- alpha-actinin binding Source: BHF-UCL
- calmodulin binding Source: GO_Central
- protein domain specific binding Source: UniProtKB
- protein homodimerization activity Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 109982, 6 interactors |
DIPi | DIP-48997N |
IntActi | Q9H2S1, 4 interactors |
STRINGi | 9606.ENSP00000427120 |
Chemistry databases
BindingDBi | Q9H2S1 |
Miscellaneous databases
RNActi | Q9H2S1, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q9H2S1 |
SMRi | Q9H2S1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 412 – 488 | Calmodulin-bindingBy similarityAdd BLAST | 77 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 41 – 45 | Poly-Gly | 5 | |
Compositional biasi | 51 – 58 | Poly-Ala | 8 | |
Compositional biasi | 83 – 88 | Poly-Gly | 6 | |
Compositional biasi | 91 – 102 | Poly-GlyAdd BLAST | 12 | |
Compositional biasi | 563 – 566 | Poly-Arg | 4 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3684, Eukaryota |
GeneTreei | ENSGT00950000182904 |
HOGENOMi | CLU_014617_0_1_1 |
InParanoidi | Q9H2S1 |
OrthoDBi | 907751at2759 |
PhylomeDBi | Q9H2S1 |
TreeFami | TF315015 |
Family and domain databases
InterProi | View protein in InterPro IPR004178, CaM-bd_dom IPR036122, CaM-bd_dom_sf IPR015449, K_chnl_Ca-activ_SK IPR013099, K_chnl_dom |
PANTHERi | PTHR10153, PTHR10153, 1 hit |
Pfami | View protein in Pfam PF02888, CaMBD, 1 hit PF07885, Ion_trans_2, 1 hit PF03530, SK_channel, 1 hit |
PRINTSi | PR01451, SKCHANNEL |
SMARTi | View protein in SMART SM01053, CaMBD, 1 hit |
SUPFAMi | SSF81327, SSF81327, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9H2S1-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSSCRYNGGV MRPLSNLSAS RRNLHEMDSE AQPLQPPASV GGGGGASSPS
60 70 80 90 100
AAAAAAAAVS SSAPEIVVSK PEHNNSNNLA LYGTGGGGST GGGGGGGGSG
110 120 130 140 150
HGSSSGTKSS KKKNQNIGYK LGHRRALFEK RKRLSDYALI FGMFGIVVMV
160 170 180 190 200
IETELSWGAY DKASLYSLAL KCLISLSTII LLGLIIVYHA REIQLFMVDN
210 220 230 240 250
GADDWRIAMT YERIFFICLE ILVCAIHPIP GNYTFTWTAR LAFSYAPSTT
260 270 280 290 300
TADVDIILSI PMFLRLYLIA RVMLLHSKLF TDASSRSIGA LNKINFNTRF
310 320 330 340 350
VMKTLMTICP GTVLLVFSIS LWIIAAWTVR ACERYHDQQD VTSNFLGAMW
360 370 380 390 400
LISITFLSIG YGDMVPNTYC GKGVCLLTGI MGAGCTALVV AVVARKLELT
410 420 430 440 450
KAEKHVHNFM MDTQLTKRVK NAAANVLRET WLIYKNTKLV KKIDHAKVRK
460 470 480 490 500
HQRKFLQAIH QLRSVKMEQR KLNDQANTLV DLAKTQNIMY DMISDLNERS
510 520 530 540 550
EDFEKRIVTL ETKLETLIGS IHALPGLISQ TIRQQQRDFI EAQMESYDKH
560 570
VTYNAERSRS SSRRRRSSST APPTSSESS
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A3F2YNY5 | A0A3F2YNY5_HUMAN | Small conductance calcium-activated... | KCNN2 | 857 | Annotation score: | ||
A0A0J9YW81 | A0A0J9YW81_HUMAN | Small conductance calcium-activated... | KCNN2 | 595 | Annotation score: | ||
A0A669KBH3 | A0A669KBH3_HUMAN | Small conductance calcium-activated... | KCNN2 | 791 | Annotation score: | ||
D6RGY7 | D6RGY7_HUMAN | Small conductance calcium-activated... | KCNN2 | 95 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 50 | S → SA in AAK84039 (Ref. 3) Curated | 1 | |
Sequence conflicti | 52 | A → D in AAG16728 (PubMed:10991935).Curated | 1 | |
Sequence conflicti | 58 | A → AA in AAP45946 (PubMed:13679367).Curated | 1 | |
Sequence conflicti | 323 | I → T in AAK84039 (Ref. 3) Curated | 1 | |
Sequence conflicti | 530 | Q → R in AAP45946 (PubMed:13679367).Curated | 1 | |
Sequence conflicti | 530 | Q → R in AAK84039 (Ref. 3) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_044584 | 1 – 348 | Missing in isoform 2. 1 PublicationAdd BLAST | 348 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF239613 mRNA Translation: AAG16728.1 AY258141 mRNA Translation: AAP45946.1 AF397175 mRNA Translation: AAK84039.1 AK289948 mRNA Translation: BAF82637.1 AC025761 Genomic DNA No translation available. AC109482 Genomic DNA No translation available. CH471086 Genomic DNA Translation: EAW48975.1 CH471086 Genomic DNA Translation: EAW48976.1 BC015371 mRNA Translation: AAH15371.1 BC117454 mRNA Translation: AAI17455.1 BC117456 mRNA Translation: AAI17457.1 |
CCDSi | CCDS43352.1 [Q9H2S1-2] |
RefSeqi | NP_001265133.1, NM_001278204.1 [Q9H2S1-2] NP_067627.2, NM_021614.3 NP_740721.1, NM_170775.2 [Q9H2S1-2] |
Genome annotation databases
Ensembli | ENST00000264773; ENSP00000264773; ENSG00000080709 [Q9H2S1-1] ENST00000503706; ENSP00000421439; ENSG00000080709 [Q9H2S1-2] ENST00000610748; ENSP00000483124; ENSG00000080709 [Q9H2S1-2] |
GeneIDi | 3781 |
KEGGi | hsa:3781 |
UCSCi | uc003kqo.4, human [Q9H2S1-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF239613 mRNA Translation: AAG16728.1 AY258141 mRNA Translation: AAP45946.1 AF397175 mRNA Translation: AAK84039.1 AK289948 mRNA Translation: BAF82637.1 AC025761 Genomic DNA No translation available. AC109482 Genomic DNA No translation available. CH471086 Genomic DNA Translation: EAW48975.1 CH471086 Genomic DNA Translation: EAW48976.1 BC015371 mRNA Translation: AAH15371.1 BC117454 mRNA Translation: AAI17455.1 BC117456 mRNA Translation: AAI17457.1 |
CCDSi | CCDS43352.1 [Q9H2S1-2] |
RefSeqi | NP_001265133.1, NM_001278204.1 [Q9H2S1-2] NP_067627.2, NM_021614.3 NP_740721.1, NM_170775.2 [Q9H2S1-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5V02 | X-ray | 1.78 | B | 395-486 | [»] | |
5WBX | X-ray | 1.90 | B | 395-486 | [»] | |
5WC5 | X-ray | 2.30 | B | 395-486 | [»] | |
6ALE | X-ray | 2.50 | B | 394-486 | [»] | |
BMRBi | Q9H2S1 | |||||
SMRi | Q9H2S1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 109982, 6 interactors |
DIPi | DIP-48997N |
IntActi | Q9H2S1, 4 interactors |
STRINGi | 9606.ENSP00000427120 |
Chemistry databases
BindingDBi | Q9H2S1 |
ChEMBLi | CHEMBL4469 |
DrugBanki | DB01110, Miconazole DB00721, Procaine DB09089, Trimebutine |
DrugCentrali | Q9H2S1 |
GuidetoPHARMACOLOGYi | 382 |
Protein family/group databases
TCDBi | 1.A.1.16.1, the voltage-gated ion channel (vic) superfamily |
PTM databases
iPTMneti | Q9H2S1 |
PhosphoSitePlusi | Q9H2S1 |
Polymorphism and mutation databases
BioMutai | KCNN2 |
DMDMi | 209572638 |
Proteomic databases
EPDi | Q9H2S1 |
jPOSTi | Q9H2S1 |
MassIVEi | Q9H2S1 |
MaxQBi | Q9H2S1 |
PaxDbi | Q9H2S1 |
PeptideAtlasi | Q9H2S1 |
PRIDEi | Q9H2S1 |
ProteomicsDBi | 67208 80583 [Q9H2S1-1] |
Protocols and materials databases
ABCDi | Q9H2S1, 1 sequenced antibody |
Antibodypediai | 13591, 120 antibodies |
Genome annotation databases
Ensembli | ENST00000264773; ENSP00000264773; ENSG00000080709 [Q9H2S1-1] ENST00000503706; ENSP00000421439; ENSG00000080709 [Q9H2S1-2] ENST00000610748; ENSP00000483124; ENSG00000080709 [Q9H2S1-2] |
GeneIDi | 3781 |
KEGGi | hsa:3781 |
UCSCi | uc003kqo.4, human [Q9H2S1-1] |
Organism-specific databases
CTDi | 3781 |
DisGeNETi | 3781 |
EuPathDBi | HostDB:ENSG00000080709.14 |
GeneCardsi | KCNN2 |
HGNCi | HGNC:6291, KCNN2 |
HPAi | ENSG00000080709, Tissue enhanced (adrenal gland, brain, liver) |
MIMi | 605879, gene |
neXtProti | NX_Q9H2S1 |
OpenTargetsi | ENSG00000080709 |
PharmGKBi | PA30071 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3684, Eukaryota |
GeneTreei | ENSGT00950000182904 |
HOGENOMi | CLU_014617_0_1_1 |
InParanoidi | Q9H2S1 |
OrthoDBi | 907751at2759 |
PhylomeDBi | Q9H2S1 |
TreeFami | TF315015 |
Enzyme and pathway databases
PathwayCommonsi | Q9H2S1 |
Reactomei | R-HSA-1296052, Ca2+ activated K+ channels |
SIGNORi | Q9H2S1 |
Miscellaneous databases
BioGRID-ORCSi | 3781, 1 hit in 842 CRISPR screens |
ChiTaRSi | KCNN2, human |
GeneWikii | KCNN2 |
GenomeRNAii | 3781 |
Pharosi | Q9H2S1, Tchem |
PROi | PR:Q9H2S1 |
RNActi | Q9H2S1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000080709, Expressed in right adrenal gland and 171 other tissues |
ExpressionAtlasi | Q9H2S1, baseline and differential |
Genevisiblei | Q9H2S1, HS |
Family and domain databases
InterProi | View protein in InterPro IPR004178, CaM-bd_dom IPR036122, CaM-bd_dom_sf IPR015449, K_chnl_Ca-activ_SK IPR013099, K_chnl_dom |
PANTHERi | PTHR10153, PTHR10153, 1 hit |
Pfami | View protein in Pfam PF02888, CaMBD, 1 hit PF07885, Ion_trans_2, 1 hit PF03530, SK_channel, 1 hit |
PRINTSi | PR01451, SKCHANNEL |
SMARTi | View protein in SMART SM01053, CaMBD, 1 hit |
SUPFAMi | SSF81327, SSF81327, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KCNN2_HUMAN | |
Accessioni | Q9H2S1Primary (citable) accession number: Q9H2S1 Secondary accession number(s): A6NF94 Q6X2Y2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 16, 2001 |
Last sequence update: | October 14, 2008 | |
Last modified: | December 2, 2020 | |
This is version 171 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 5
Human chromosome 5: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families