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Protein

Ras association domain-containing protein 4

Gene

RASSF4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Potential tumor suppressor. May act as a KRAS effector protein. May promote apoptosis and cell cycle arrest.1 Publication

GO - Biological processi

Keywordsi

Biological processCell cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Ras association domain-containing protein 4
Gene namesi
Name:RASSF4
ORF Names:AD037
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000107551.20
HGNCiHGNC:20793 RASSF4
MIMi610559 gene
neXtProtiNX_Q9H2L5

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

Organism-specific databases

DisGeNETi83937
OpenTargetsiENSG00000107551
PharmGKBiPA134951366

Polymorphism and mutation databases

BioMutaiRASSF4
DMDMi109892952

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002403981 – 321Ras association domain-containing protein 4Add BLAST321

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei141PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9H2L5
MaxQBiQ9H2L5
PaxDbiQ9H2L5
PeptideAtlasiQ9H2L5
PRIDEiQ9H2L5
ProteomicsDBi80562
80563 [Q9H2L5-2]
80564 [Q9H2L5-3]
80565 [Q9H2L5-4]

PTM databases

iPTMnetiQ9H2L5
PhosphoSitePlusiQ9H2L5

Expressioni

Tissue specificityi

Widely expressed. Frequently down-regulated in tumor cell lines.1 Publication

Gene expression databases

BgeeiENSG00000107551 Expressed in 203 organ(s), highest expression level in left lobe of thyroid gland
CleanExiHS_RASSF4
ExpressionAtlasiQ9H2L5 baseline and differential
GenevisibleiQ9H2L5 HS

Organism-specific databases

HPAiHPA038834

Interactioni

Subunit structurei

Interacts directly with activated KRAS in a GTP-dependent manner.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi123820, 3 interactors
IntActiQ9H2L5, 8 interactors
STRINGi9606.ENSP00000339692

Structurei

3D structure databases

ProteinModelPortaliQ9H2L5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini174 – 262Ras-associatingPROSITE-ProRule annotationAdd BLAST89
Domaini270 – 317SARAHPROSITE-ProRule annotationAdd BLAST48

Phylogenomic databases

eggNOGiKOG4239 Eukaryota
ENOG410Y2N1 LUCA
GeneTreeiENSGT00510000046426
HOGENOMiHOG000231738
HOVERGENiHBG054302
InParanoidiQ9H2L5
KOiK09851
OMAiMFLMEAD
OrthoDBiEOG091G0BOA
PhylomeDBiQ9H2L5
TreeFamiTF319243

Family and domain databases

InterProiView protein in InterPro
IPR033614 C-RASSF
IPR000159 RA_dom
IPR033622 RASSF4
IPR011524 SARAH_dom
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR22738 PTHR22738, 1 hit
PTHR22738:SF4 PTHR22738:SF4, 1 hit
PfamiView protein in Pfam
PF16517 Nore1-SARAH, 1 hit
PF00788 RA, 1 hit
SMARTiView protein in SMART
SM00314 RA, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50200 RA, 1 hit
PS50951 SARAH, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9H2L5-1) [UniParc]FASTAAdd to basket
Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKEDCLPSSH VPISDSKSIQ KSELLGLLKT YNCYHEGKSF QLRHREEEGT
60 70 80 90 100
LIIEGLLNIA WGLRRPIRLQ MQDDREQVHL PSTSWMPRRP SCPLKEPSPQ
110 120 130 140 150
NGNITAQGPS IQPVHKAESS TDSSGPLEEA EEAPQLMRTK SDASCMSQRR
160 170 180 190 200
PKCRAPGEAQ RIRRHRFSIN GHFYNHKTSV FTPAYGSVTN VRVNSTMTTL
210 220 230 240 250
QVLTLLLNKF RVEDGPSEFA LYIVHESGER TKLKDCEYPL ISRILHGPCE
260 270 280 290 300
KIARIFLMEA DLGVEVPHEV AQYIKFEMPV LDSFVEKLKE EEEREIIKLT
310 320
MKFQALRLTM LQRLEQLVEA K
Length:321
Mass (Da):36,748
Last modified:June 27, 2006 - v2
Checksum:i8EB5990165886D99
GO
Isoform 2 (identifier: Q9H2L5-2) [UniParc]FASTAAdd to basket
Also known as: C

The sequence of this isoform differs from the canonical sequence as follows:
     29-46: Missing.
     94-94: L → LGCWSLLLGLSSLSLPAAISALQLSVFR

Show »
Length:330
Mass (Da):37,255
Checksum:i1A4C69ED22754F32
GO
Isoform 3 (identifier: Q9H2L5-3) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.

Show »
Length:251
Mass (Da):28,689
Checksum:i29DEAD2F42FE7C1E
GO
Isoform 4 (identifier: Q9H2L5-4) [UniParc]FASTAAdd to basket
Also known as: D

The sequence of this isoform differs from the canonical sequence as follows:
     95-126: KEPSPQNGNITAQGPSIQPVHKAESSTDSSGP → APGGGRGGPPADADQERRQLHEPEEAQ
     127-321: Missing.

Show »
Length:121
Mass (Da):13,735
Checksum:i8F3E99BD8313440E
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
V9GYK1V9GYK1_HUMAN
Ras association domain-containing p...
RASSF4
84Annotation score:
Q5T737Q5T737_HUMAN
Ras association domain-containing p...
RASSF4
123Annotation score:
Q5T739Q5T739_HUMAN
Ras association domain-containing p...
RASSF4
151Annotation score:
R4GNA4R4GNA4_HUMAN
Ras association domain-containing p...
RASSF4
49Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti107Q → K in AAG44666 (Ref. 1) Curated1
Sequence conflicti107Q → K in AAO61138 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03443810H → Y. Corresponds to variant dbSNP:rs34692238Ensembl.1
Natural variantiVAR_03443988R → G. Corresponds to variant dbSNP:rs870957Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0193551 – 70Missing in isoform 3. 1 PublicationAdd BLAST70
Alternative sequenceiVSP_01935629 – 46Missing in isoform 2. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_01935794L → LGCWSLLLGLSSLSLPAAIS ALQLSVFR in isoform 2. 1 Publication1
Alternative sequenceiVSP_01935895 – 126KEPSP…DSSGP → APGGGRGGPPADADQERRQL HEPEEAQ in isoform 4. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_019359127 – 321Missing in isoform 4. 1 PublicationAdd BLAST195

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY216713 mRNA Translation: AAO61138.1
AY216714 mRNA Translation: AAO61139.1
AY216715 mRNA Translation: AAO61140.1
AY216716 mRNA Translation: AAO61141.1
AF260335 mRNA Translation: AAG44666.1
AL353801 Genomic DNA No translation available.
BC032593 mRNA Translation: AAH32593.1
AL713716 mRNA Translation: CAD28511.1
CCDSiCCDS7208.1 [Q9H2L5-1]
RefSeqiNP_114412.2, NM_032023.3 [Q9H2L5-1]
UniGeneiHs.522895

Genome annotation databases

EnsembliENST00000340258; ENSP00000339692; ENSG00000107551 [Q9H2L5-1]
GeneIDi83937
KEGGihsa:83937
UCSCiuc001jbo.4 human [Q9H2L5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY216713 mRNA Translation: AAO61138.1
AY216714 mRNA Translation: AAO61139.1
AY216715 mRNA Translation: AAO61140.1
AY216716 mRNA Translation: AAO61141.1
AF260335 mRNA Translation: AAG44666.1
AL353801 Genomic DNA No translation available.
BC032593 mRNA Translation: AAH32593.1
AL713716 mRNA Translation: CAD28511.1
CCDSiCCDS7208.1 [Q9H2L5-1]
RefSeqiNP_114412.2, NM_032023.3 [Q9H2L5-1]
UniGeneiHs.522895

3D structure databases

ProteinModelPortaliQ9H2L5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123820, 3 interactors
IntActiQ9H2L5, 8 interactors
STRINGi9606.ENSP00000339692

PTM databases

iPTMnetiQ9H2L5
PhosphoSitePlusiQ9H2L5

Polymorphism and mutation databases

BioMutaiRASSF4
DMDMi109892952

Proteomic databases

EPDiQ9H2L5
MaxQBiQ9H2L5
PaxDbiQ9H2L5
PeptideAtlasiQ9H2L5
PRIDEiQ9H2L5
ProteomicsDBi80562
80563 [Q9H2L5-2]
80564 [Q9H2L5-3]
80565 [Q9H2L5-4]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000340258; ENSP00000339692; ENSG00000107551 [Q9H2L5-1]
GeneIDi83937
KEGGihsa:83937
UCSCiuc001jbo.4 human [Q9H2L5-1]

Organism-specific databases

CTDi83937
DisGeNETi83937
EuPathDBiHostDB:ENSG00000107551.20
GeneCardsiRASSF4
HGNCiHGNC:20793 RASSF4
HPAiHPA038834
MIMi610559 gene
neXtProtiNX_Q9H2L5
OpenTargetsiENSG00000107551
PharmGKBiPA134951366
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4239 Eukaryota
ENOG410Y2N1 LUCA
GeneTreeiENSGT00510000046426
HOGENOMiHOG000231738
HOVERGENiHBG054302
InParanoidiQ9H2L5
KOiK09851
OMAiMFLMEAD
OrthoDBiEOG091G0BOA
PhylomeDBiQ9H2L5
TreeFamiTF319243

Miscellaneous databases

ChiTaRSiRASSF4 human
GenomeRNAii83937
PROiPR:Q9H2L5
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000107551 Expressed in 203 organ(s), highest expression level in left lobe of thyroid gland
CleanExiHS_RASSF4
ExpressionAtlasiQ9H2L5 baseline and differential
GenevisibleiQ9H2L5 HS

Family and domain databases

InterProiView protein in InterPro
IPR033614 C-RASSF
IPR000159 RA_dom
IPR033622 RASSF4
IPR011524 SARAH_dom
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR22738 PTHR22738, 1 hit
PTHR22738:SF4 PTHR22738:SF4, 1 hit
PfamiView protein in Pfam
PF16517 Nore1-SARAH, 1 hit
PF00788 RA, 1 hit
SMARTiView protein in SMART
SM00314 RA, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50200 RA, 1 hit
PS50951 SARAH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRASF4_HUMAN
AccessioniPrimary (citable) accession number: Q9H2L5
Secondary accession number(s): Q86WH5
, Q86WH6, Q86WH7, Q8N5A9, Q8TCK6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: October 10, 2018
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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