UniProtKB - Q9H2J4 (PDCL3_HUMAN)
Protein
Phosducin-like protein 3
Gene
PDCL3
Organism
Homo sapiens (Human)
Status
Functioni
Acts as a chaperone for the angiogenic VEGF receptor KDR/VEGFR2, increasing its abundance by inhibiting its ubiquitination and degradation (PubMed:23792958, PubMed:26059764). Inhibits the folding activity of the chaperonin-containing T-complex (CCT) which leads to inhibition of cytoskeletal actin folding (PubMed:17429077). Acts as a chaperone during heat shock alongside HSP90 and HSP40/70 chaperone complexes (By similarity). Modulates the activation of caspases during apoptosis (PubMed:15371430).By similarity4 Publications
GO - Molecular functioni
- protein folding chaperone Source: UniProtKB
- vascular endothelial growth factor receptor 2 binding Source: Ensembl
GO - Biological processi
- actin cytoskeleton organization Source: UniProtKB
- angiogenesis Source: UniProtKB
- apoptotic process Source: UniProtKB-KW
- cellular response to heat Source: Ensembl
- chaperone-mediated protein folding Source: UniProtKB
- negative regulation of chaperone-mediated protein folding Source: UniProtKB
- negative regulation of ubiquitin-dependent protein catabolic process Source: MGI
- positive regulation of angiogenesis Source: MGI
- positive regulation of endothelial cell proliferation Source: MGI
- positive regulation of gene expression Source: UniProtKB
- protein stabilization Source: UniProtKB
- regulation of peptidyl-tyrosine phosphorylation Source: MGI
Keywordsi
Molecular function | Chaperone |
Biological process | Angiogenesis, Apoptosis |
Enzyme and pathway databases
PathwayCommonsi | Q9H2J4 |
Names & Taxonomyi
Protein namesi | Recommended name: Phosducin-like protein 3Alternative name(s): HTPHLP PhPL3 Viral IAP-associated factor 11 Publication Short name: VIAF-11 Publication |
Gene namesi | Name:PDCL3 Synonyms:PhLP2A, VIAF1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28860, PDCL3 |
MIMi | 611678, gene |
neXtProti | NX_Q9H2J4 |
VEuPathDBi | HostDB:ENSG00000115539.13 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Other locations
- perinuclear region 1 Publication
Cytosol
- cytosol Source: HPA
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
Nucleus
- nucleoplasm Source: HPA
- perinucleolar compartment Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
- protein-containing complex Source: Ensembl
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulumPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1 | Missing : Loss of acetylation. Increases protein stability. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 79031 |
OpenTargetsi | ENSG00000115539 |
PharmGKBi | PA134979849 |
Miscellaneous databases
Pharosi | Q9H2J4, Tbio |
Genetic variation databases
BioMutai | PDCL3 |
DMDMi | 50401164 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000163758 | 1 – 239 | Phosducin-like protein 3Add BLAST | 239 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionine1 Publication | 1 | |
Modified residuei | 43 | PhosphoserineCombined sources | 1 | |
Modified residuei | 234 | PhosphoserineCombined sources | 1 | |
Modified residuei | 236 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
N-terminal methionine acetylation destabilizes the protein.1 Publication
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9H2J4 |
jPOSTi | Q9H2J4 |
MassIVEi | Q9H2J4 |
MaxQBi | Q9H2J4 |
PaxDbi | Q9H2J4 |
PeptideAtlasi | Q9H2J4 |
PRIDEi | Q9H2J4 |
ProteomicsDBi | 80555 |
TopDownProteomicsi | Q9H2J4 |
PTM databases
iPTMneti | Q9H2J4 |
MetOSitei | Q9H2J4 |
PhosphoSitePlusi | Q9H2J4 |
Expressioni
Tissue specificityi
Expressed in endothelial cells (at protein level) (PubMed:26059764). Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine and colon (PubMed:15371430).2 Publications
Gene expression databases
Bgeei | ENSG00000115539, Expressed in hypothalamus and 119 other tissues |
ExpressionAtlasi | Q9H2J4, baseline and differential |
Genevisiblei | Q9H2J4, HS |
Organism-specific databases
HPAi | ENSG00000115539, Low tissue specificity |
Interactioni
Subunit structurei
Interacts (via thioredoxin fold region) with KDR/VEGFR2 (via juxtamembrane domain) (PubMed:23792958, PubMed:26059764). Forms ternary complexes with the chaperonin CCT complex and actin substrate, leading to inhibition of actin folding (PubMed:17429077).
Interacts with XIAP (via BIR 3 and RING domain) (PubMed:19012568).
Interacts with HSP90AA1 and HSP90AB1 (By similarity).
By similarity4 PublicationsGO - Molecular functioni
- vascular endothelial growth factor receptor 2 binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 122497, 67 interactors |
IntActi | Q9H2J4, 20 interactors |
STRINGi | 9606.ENSP00000264254 |
Chemistry databases
BindingDBi | Q9H2J4 |
Miscellaneous databases
RNActi | Q9H2J4, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 91 – 239 | Thioredoxin foldBy similarityAdd BLAST | 149 | |
Regioni | 97 – 99 | Interaction with XIAP1 Publication | 3 | |
Regioni | 153 – 155 | Interaction with XIAP1 Publication | 3 |
Sequence similaritiesi
Belongs to the phosducin family.Curated
Phylogenomic databases
eggNOGi | KOG3170, Eukaryota |
GeneTreei | ENSGT00940000154295 |
HOGENOMi | CLU_072604_0_0_1 |
InParanoidi | Q9H2J4 |
OMAi | SGQDYVK |
OrthoDBi | 1247977at2759 |
PhylomeDBi | Q9H2J4 |
TreeFami | TF315179 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR024253, Phosducin_thioredoxin-like_dom IPR036249, Thioredoxin-like_sf |
Pfami | View protein in Pfam PF02114, Phosducin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9H2J4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQDPNADTEW NDILRKKGIL PPKESLKELE EEAEEEQRIL QQSVVKTYED
60 70 80 90 100
MTLEELEDHE DEFNEEDERA IEMYRRRRLA EWKATKLKNK FGEVLEISGK
110 120 130 140 150
DYVQEVTKAG EGLWVILHLY KQGIPLCALI NQHLSGLARK FPDVKFIKAI
160 170 180 190 200
STTCIPNYPD RNLPTIFVYL EGDIKAQFIG PLVFGGMNLT RDELEWKLSE
210 220 230
SGAIMTDLEE NPKKPIEDVL LSSVRRSVLM KRDSDSEGD
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JST4 | C9JST4_HUMAN | Phosducin-like protein 3 | PDCL3 | 87 | Annotation score: | ||
H7BZP2 | H7BZP2_HUMAN | Phosducin-like protein 3 | PDCL3 | 150 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF110511 mRNA Translation: AAG21887.1 AF267853 mRNA Translation: AAG44722.1 AK313985 mRNA Translation: BAG36697.1 AC068538 Genomic DNA Translation: AAX93227.1 CH471127 Genomic DNA Translation: EAX01838.1 BC001021 mRNA Translation: AAH01021.1 |
CCDSi | CCDS33261.1 |
RefSeqi | NP_076970.1, NM_024065.4 |
Genome annotation databases
Ensembli | ENST00000264254; ENSP00000264254; ENSG00000115539 |
GeneIDi | 79031 |
KEGGi | hsa:79031 |
UCSCi | uc002tao.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF110511 mRNA Translation: AAG21887.1 AF267853 mRNA Translation: AAG44722.1 AK313985 mRNA Translation: BAG36697.1 AC068538 Genomic DNA Translation: AAX93227.1 CH471127 Genomic DNA Translation: EAX01838.1 BC001021 mRNA Translation: AAH01021.1 |
CCDSi | CCDS33261.1 |
RefSeqi | NP_076970.1, NM_024065.4 |
3D structure databases
BMRBi | Q9H2J4 |
SMRi | Q9H2J4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 122497, 67 interactors |
IntActi | Q9H2J4, 20 interactors |
STRINGi | 9606.ENSP00000264254 |
Chemistry databases
BindingDBi | Q9H2J4 |
PTM databases
iPTMneti | Q9H2J4 |
MetOSitei | Q9H2J4 |
PhosphoSitePlusi | Q9H2J4 |
Genetic variation databases
BioMutai | PDCL3 |
DMDMi | 50401164 |
Proteomic databases
EPDi | Q9H2J4 |
jPOSTi | Q9H2J4 |
MassIVEi | Q9H2J4 |
MaxQBi | Q9H2J4 |
PaxDbi | Q9H2J4 |
PeptideAtlasi | Q9H2J4 |
PRIDEi | Q9H2J4 |
ProteomicsDBi | 80555 |
TopDownProteomicsi | Q9H2J4 |
Protocols and materials databases
Antibodypediai | 17722, 175 antibodies |
DNASUi | 79031 |
Genome annotation databases
Ensembli | ENST00000264254; ENSP00000264254; ENSG00000115539 |
GeneIDi | 79031 |
KEGGi | hsa:79031 |
UCSCi | uc002tao.3, human |
Organism-specific databases
CTDi | 79031 |
DisGeNETi | 79031 |
GeneCardsi | PDCL3 |
HGNCi | HGNC:28860, PDCL3 |
HPAi | ENSG00000115539, Low tissue specificity |
MIMi | 611678, gene |
neXtProti | NX_Q9H2J4 |
OpenTargetsi | ENSG00000115539 |
PharmGKBi | PA134979849 |
VEuPathDBi | HostDB:ENSG00000115539.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3170, Eukaryota |
GeneTreei | ENSGT00940000154295 |
HOGENOMi | CLU_072604_0_0_1 |
InParanoidi | Q9H2J4 |
OMAi | SGQDYVK |
OrthoDBi | 1247977at2759 |
PhylomeDBi | Q9H2J4 |
TreeFami | TF315179 |
Enzyme and pathway databases
PathwayCommonsi | Q9H2J4 |
Miscellaneous databases
BioGRID-ORCSi | 79031, 98 hits in 990 CRISPR screens |
ChiTaRSi | PDCL3, human |
GenomeRNAii | 79031 |
Pharosi | Q9H2J4, Tbio |
PROi | PR:Q9H2J4 |
RNActi | Q9H2J4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000115539, Expressed in hypothalamus and 119 other tissues |
ExpressionAtlasi | Q9H2J4, baseline and differential |
Genevisiblei | Q9H2J4, HS |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR024253, Phosducin_thioredoxin-like_dom IPR036249, Thioredoxin-like_sf |
Pfami | View protein in Pfam PF02114, Phosducin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PDCL3_HUMAN | |
Accessioni | Q9H2J4Primary (citable) accession number: Q9H2J4 Secondary accession number(s): B2RA00, Q53S68 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | March 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families